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basR basR eptA eptA pmrR pmrR surA surA lpxC lpxC dksA dksA lpxD lpxD fabZ fabZ lpxA lpxA lpxB lpxB prpB prpB lacZ lacZ phoB phoB lpxH lpxH pagP pagP clsB clsB opgE opgE lrp lrp ycaI ycaI msbA msbA lpxK lpxK csgD csgD clsC clsC lpxL lpxL phoP phoP oppA oppA yciS yciS yciM yciM lpxM lpxM arnB arnB arnT arnT lpxP lpxP rpoE rpoE ftsH ftsH eptB eptB hldD hldD waaF waaF waaC waaC waaL waaL waaZ waaZ waaP waaP waaG waaG waaQ waaQ basS basS
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query proteins and first shell of interactors
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second shell of interactors
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proteins of unknown 3D structure
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a 3D structure is known or predicted
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basRResponse regulator in two-component regulatory system with BasS; Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin (By similarity). (222 aa)
eptALipid A phosphoethanolamine transferase; Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin; Belongs to the phosphoethanolamine transferase family. EptA subfamily. (547 aa)
pmrRPutative membrane-bound BasS regulator; May bind to BasS and modulate its sensor kinase activity. (29 aa)
surAPeptidyl-prolyl cis-trans isomerase (PPIase); Chaperone involved in the correct folding and assembly of outer membrane proteins, such as OmpA, OmpF and LamB. Recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins. May act in both early periplasmic and late outer membrane- associated steps of protein maturation. Essential for the survival of E.coli in stationary phase. Required for pilus biogenesis. (428 aa)
lpxCUDP-3-O-acyl N-acetylglucosamine deacetylase; Catalyzes the hydrolysis of UDP-3-O-myristoyl-N- acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis. (305 aa)
dksATranscriptional regulator of rRNA transcription; Transcription factor that acts by binding directly to the RNA polymerase (RNAP). Required for negative regulation of rRNA expression and positive regulation of several amino acid biosynthesis promoters. Also required for regulation of fis expression. Binding to RNAP disrupts interaction of RNAP with DNA, inhibits formation of initiation complexes, and amplifies effects of ppGpp and the initiating NTP on rRNA transcription. Inhibits transcript elongation, exonucleolytic RNA cleavage and pyrophosphorolysis, and increases intrinsic terminat [...] (151 aa)
lpxDUDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase; Catalyzes the N-acylation of UDP-3-O- (hydroxytetradecanoyl)glucosamine using 3-hydroxytetradecanoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. Prefers (3R)-3-hydroxytetradecanoyl-ACP over (3R)-3-hydroxyhexadecanoyl-ACP as the acyl donor in vitro, which is consistent with the structure of E.coli lipid A that contains over 95% (R)-3-hydroxytetradecanoate at the 2 and 2' positions. Belongs to the transferase [...] (341 aa)
fabZ(3R)-hydroxymyristol acyl carrier protein dehydratase; Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs. (151 aa)
lpxAUDP-N-acetylglucosamine acetyltransferase; Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. (262 aa)
lpxBtetraacyldisaccharide-1-P synthase; Condensation of UDP-2,3-diacylglucosamine and 2,3- diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell; Belongs to the LpxB family. (382 aa)
prpB2-methylisocitrate lyase; Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2- methylisocitrate to yield pyruvate and succinate via an alpha-carboxy- carbanion intermediate; Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family. (296 aa)
lacZbeta-D-galactosidase; Protein involved in carbohydrate catabolic process; Belongs to the glycosyl hydrolase 2 family. (1024 aa)
phoBResponse regulator in two-component regulatory system with PhoR; This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited. (229 aa)
lpxHUDP-2,3-diacylglucosamine pyrophosphohydrolase; Hydrolyzes the pyrophosphate bond of UDP-2,3- diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP by catalyzing the attack of water at the alpha-P atom. Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. Is essential for E.coli growth. Does not cleave the unacylated UDP-GlcNAc, the mono-acylated UDP-3-O-(R)-3- hydroxymyristoyl-GlcNAc, and CDP-diacylglycerol. Belongs to the LpxH family. (240 aa)
pagPPhospholipid:lipid A palmitoyltransferase; Transfers a palmitate residue from the sn-1 position of a phospholipid to the N-linked hydroxymyristate on the proximal unit of lipid A or its precursors. Phosphatidylglycerol (PtdGro), phosphatidylethanolamine (PtdEtn), phosphatidylserine (PtdSer) and phosphatidic acid (Ptd-OH) are all effective acyl donors. (186 aa)
clsBCardiolipin synthase 2; Catalyzes the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. Can also catalyze phosphatidyl group transfer to water to form phosphatidate. Belongs to the phospholipase D family. Cardiolipin synthase subfamily. ClsB sub-subfamily. (413 aa)
opgEOPG biosynthetic transmembrane phosphoethanolamine transferase; Catalyzes the addition of a phosphoethanolamine moiety to the osmoregulated periplasmic glucan (OPG) backbone. (527 aa)
lrpLeucine-responsive global transcriptional regulator; Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes. (164 aa)
ycaIComEC family inner membrane protein. (754 aa)
msbALipid ABC transporter permease/ATPase; Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation. Belongs to the ABC transporter superfamily. Lipid exporter (TC 3.A.1.106) family. (582 aa)
lpxKLipid A 4'kinase; Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA). (328 aa)
csgDcsgBAC operon transcriptional regulator; The master regulator for adhesive curli fimbriae expression; necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. May have the capability to respond to starvation and/or high cell density by activating csgBA transcription. Low-level constitutive expression confers an adherent curli fimbriae- expressing phenotype, up-regulates 10 genes and down-regulates 14 others. (216 aa)
clsCStationary phase cardiolipin synthase 3; Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) from phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). (473 aa)
lpxLLauryl-acyl carrier protein (ACP)-dependent acyltransferase; Catalyzes the transfer of laurate from lauroyl-acyl carrier protein (ACP) to Kdo(2)-lipid IV(A) to form Kdo(2)-(lauroyl)-lipid IV(A). Has 10 fold selectivity for lauroyl-ACP over myristoyl-ACP. In vitro, can also catalyze a slow second acylation reaction leading to the formation of Kdo(2)-(dilauroyl)-lipid IV(A). (306 aa)
phoPResponse regulator in two-component regulatory system with PhoQ; Member of the two-component regulatory system PhoP/PhoQ involved in adaptation to low Mg(2+) environments and the control of acid resistance genes. In low periplasmic Mg(2+), PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG (By similarity). Mediates magnesium influx to the cytosol by activation of MgtA. Pro [...] (223 aa)
oppAOligopeptide ABC transporter periplasmic binding protein; This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity; Belongs to the bacterial solute-binding protein 5 family. (543 aa)
yciSDUF1049 family inner membrane protein, function unknown; Involved in the assembly of lipopolysaccharide (LPS). Belongs to the LapA family. (102 aa)
yciMLPS regulatory protein; Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. May act by modulating the proteolytic activity of FtsH towards LpxC. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane. (389 aa)
lpxMMyristoyl-acyl carrier protein (ACP)-dependent acyltransferase; Catalyzes the transfer of myristate from myristoyl-acyl carrier protein (ACP) to Kdo(2)-(lauroyl)-lipid IV(A) to form Kdo(2)- lipid A. Can probably also catalyze the transfer of myristate to Kdo(2)-(palmitoleoyl)-lipid IV(A) to form the cold-adapted Kdo(2)-lipid A. In vitro, can acylate Kdo(2)-lipid IV(A), but acylation of (KDO)2- (lauroyl)-lipid IV(A) is about 100 times faster. In vitro, can use lauroyl-ACP but displays a slight kinetic preference for myristoyl-ACP. (323 aa)
arnBUridine 5'-(beta-1-threo-pentapyranosyl-4-ulose diphosphate) aminotransferase, PLP-dependent; Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides. (379 aa)
arnT4-amino-4-deoxy-L-arabinose transferase; Catalyzes the transfer of the L-Ara4N moiety of the glycolipid undecaprenyl phosphate-alpha-L-Ara4N to lipid A. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides. (550 aa)
lpxPPalmitoleoyl-acyl carrier protein (ACP)-dependent acyltransferase; Catalyzes the transfer of palmitoleate from palmitoleoyl-acyl carrier protein (ACP) to Kdo(2)-lipid IV(A) to form Kdo(2)- (palmitoleoyl)-lipid IV(A). Required for the biosynthesis of a distinct molecular species of lipid A, which is present only in cells grown at low temperatures. It may confer a selective advantage to cells growing at lower temperatures by making the outer membrane a more effective barrier to harmful chemicals. (306 aa)
rpoERNA polymerase sigma E factor; Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma-E controls the envelope stress response, responding to periplasmic protein stress, increased levels of periplasmic lipopolysaccharide (LPS) as well as heat shock and oxidative stress; it controls protein processing in the extracytoplasmic compartment. The 90 member regulon consists of the genes necessary for the synthesis and maintenance of both proteins and LPS of the outer me [...] (191 aa)
ftsHProtease, ATP-dependent zinc-metallo; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins. Degrades a few membrane proteins that have not been assembled into complexes such as SecY, F(0) ATPase subunit a and YccA, and also cytoplasmic proteins sigma-32, LpxC, KdtA and phage lambda cII protein among others. Degrades membrane proteins in a processive manner starting at either the N- or C-terminus; recognition requires a cytoplasmic tail of about 20 residues with no apparent [...] (644 aa)
eptBKDO phosphoethanolamine transferase, Ca(2+)-inducible; Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (Kdo) residue of a Kdo(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases diacylglycerol; Belongs to the phosphoethanolamine transferase family. EptB subfamily. (563 aa)
hldDADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding; Catalyzes the interconversion between ADP-D-glycero-beta-D- manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose; Belongs to the NAD(P)-dependent epimerase/dehydratase family. HldD subfamily. (310 aa)
waaFADP-heptose--lps heptosyltransferase II; lipopolysaccharide core biosynthesis; Protein involved in cell surface antigen activity, host-interacting and lipopolysaccharide core region biosynthetic process. (348 aa)
waaCADP-heptose:LPS heptosyl transferase I; Heptose transfer to the lipopolysaccharide core. It transfers the innermost heptose to [4'-P](3-deoxy-D-manno-octulosonic acid)2-IVA; Belongs to the glycosyltransferase 9 family. (319 aa)
waaLO-antigen ligase; Adds the O-antigen on the glucose group of LPS. (419 aa)
waaZLipopolysaccharide KdoIII transferase; Lipopolysaccharide core biosynthesis; Protein involved in cell surface antigen activity, host-interacting and lipopolysaccharide core region biosynthetic process. (283 aa)
waaPKinase that phosphorylates core heptose of lipopolysaccharide; Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core. (265 aa)
waaGUDP-glucose:(heptosyl)lipopolysaccharide alpha-1,3-glucosyltransferase; Involved in the addition of the first glucose residue to the lipopolysaccharide core; Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. (374 aa)
waaQLipopolysaccharide core biosynthesis protein; Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core (By similarity); Belongs to the glycosyltransferase 9 family. (344 aa)
basSSensory histidine kinase in two-component regulatory system with BasR; Member of the two-component regulatory system BasS/BasR Autophosphorylates and activates BasR by phosphorylation. (363 aa)
Your Current Organism:
Escherichia coli K12
NCBI taxonomy Id: 511145
Other names: E. coli str. K-12 substr. MG1655, Escherichia coli MG1655, Escherichia coli str. K-12 substr. MG1655, Escherichia coli str. K12 substr. MG1655, Escherichia coli str. MG1655, Escherichia coli strain MG1655
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