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ligM | vanillate/3-O-methylgallate O-demethylase; Involved in the catabolism of vanillate and syringate. Catalyzes the transfer of a methyl moiety from vanillate or 3-O- methylgallate (3MGA) to tetrahydrofolate, forming protocatechuate (PCA) or gallate, respectively, and methyl-tetrahydrofolate. Has similar activities with both substrates. Cannot use syringate. Uses an ordered, sequential kinetic mechanism. (471 aa) | ||||
folD | Methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. (298 aa) | ||||
desB | Gallate dioxygenase. (418 aa) | ||||
ligC | 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase; Involved in the degradation of protocatechuate (PCA) via the PCA 4,5-cleavage pathway. Catalyzes the oxidation of the hemiacetal form of 4-carboxy-2-hydroxymuconate-6-semialdehyde (CHMS) to produce 2- pyrone-4,6-dicarboxylate (PDC). LigC has 10-times-higher affinity to NADP than to NAD. (315 aa) | ||||
ligB | Protocatechuate 4,5-dioxygenase beta chain; Responsible for the aromatic ring fission of protocatechuate. (302 aa) | ||||
ligJ | 4-oxalomesaconate hydratase; Contributes to the degradation of lignin at the level of the protocatechuate 4,5-cleavage pathway. Catalyzes the hydration of the double bond of (3Z)-2-keto-4-carboxy-3-hexenedioate (KCH) to (4S)-4- carboxy-4-hydroxy-2-oxoadipate (CHA, also named (2S)-2-hydroxy-4- oxobutane-1,2,4-tricarboxylate). Is involved in the catabolism of both vanillate and syringate. (341 aa) | ||||
ligR | LysR family transcriptional regulator; Belongs to the LysR transcriptional regulatory family. (393 aa) | ||||
ligK | 4-hydroxy-4-methyl-2-oxoadipate aldolase; Contributes to the degradation of lignin, being involved in the final step of the protocatechuate 4,5-cleavage pathway. Catalyzes the conversion of 4-carboxy-4-hydroxy-2-oxoadipate (CHA, also named 2- hydroxy-4-oxobutane-1,2,4-tricarboxylate) to pyruvate and oxaloacetate but also the decarboxylation of oxaloacetate to pyruvate and CO2. Is essential for catabolism and utilization of vanillate and syringate. Is also able to catalyze the formation of 4-hydroxy- 4-methyl-2-oxoglutarate (HMG) from two molecules of pyruvate ; Belongs to the LigK/PcmE [...] (228 aa) | ||||
ligI | 2-pyrone-4,6-dicarboxylic acid hydrolase; Contributes to the degradation of lignin at the level of the protocatechuate 4,5-cleavage pathway. Catalyzes the hydrolysis of 2-pyrone-4,6-dicarboxylate (PDC) to (4E)-oxalomesaconate (OMA). The keto form of OMA can tautomerize into the enol form, 4-carboxy-2-hydroxymuconate (CHM), under certain pH conditions. Also catalyzes the reverse reaction. Is essential for the growth of Sphingobium sp. SYK-6 on vanillate but is not responsible for the growth of this strain on syringate. Belongs to the metallo-dependent hydrolases superfamily. PDC hydrola [...] (293 aa) | ||||
metF | 5,10-methylenetetrahydrofolate reductase; Probably catalyzes the conversion of methyl-tetrahydrofolate to methylene-tetrahydrofolate. Required for tetrahydrofolate-dependent vanillate and syringate O-demethylation; Belongs to the methylenetetrahydrofolate reductase family. (288 aa) | ||||
ligH | 10-formyltetrahydrofolate synthetase. (558 aa) | ||||
SLG_12860 | Conserved hypothetical protein. (489 aa) | ||||
SLG_12870 | MarR family transcriptional regulator. (172 aa) | ||||
SLG_14170 | LysR family transcriptional regulator; Belongs to the LysR transcriptional regulatory family. (295 aa) | ||||
desZ | 3-O-methylgallate 3,4-dioxygenase. (330 aa) | ||||
folD-2 | Methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. (307 aa) | ||||
tig | Trigger factor; Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase; Belongs to the FKBP-type PPIase family. Tig subfamily. (532 aa) | ||||
desA | Syringate O-demethylase; Involved in the catabolism of syringate. Catalyzes the conversion of syringate to 3-O-methylgallate (3MGA) in the presence of tetrahydrofolate. Has weak activity with vanillate and 3-O- methylgallate; Belongs to the GcvT family. (462 aa) | ||||
glyA | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. (439 aa) |