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| | glpD2 | Rv3302c, (MTCI418A.04c, MTV016.01c), len: 585 aa. Probable glpd2, glycerol-3-phosphate dehydrogenase,equivalent to P53435|GLPD_MYCLE|ML0713|L308_C1_179 glycerol-3-phosphate dehydrogenase from Mycobacterium leprae (585 aa), FASTA scores: opt: 3489, E(): 2.2e-198,(90.75% identity in 584 aa overlap). Also highly similar to many e.g. Q9L0I3|SCD63.06 from Streptomyces coelicolor (568 aa), FASTA scores: opt: 2203, E(): 1.6e-122, (59.95% identity in 564 aa overlap); Q9RVK8|DR1019 from Deinococcus radiodurans (522 aa), FASTA scores: opt: 949, E(): 1.4e-48,(37.0% identity in 538 aa overlap); BA [...] (585 aa) |
| | glpQ1 | Probable glycerophosphodiester phosphodiesterase 1; Glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols. (274 aa) |
| | glf | UDP-galactopyranose mutase; Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF) which is a key building block for cell wall construction in Mycobacterium tuberculosis. (399 aa) |
| | glfT2 | Bifunctional UDP-galactofuranosyl transferase GlfT2; Involved in the galactan polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacteria cell wall. Thus, successively transfers approximately 28 galactofuranosyl (Galf) residues from UDP-galactofuranose (UDP-Galf) onto the galactofuranosyl- galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha- GlcNAc-PP-C50) acceptor produced by GlfT1, with alternating 1->5 and 1->6 links, forming a galactan domain with approximately 30 galactof [...] (637 aa) |
| | Rv3807c | Possible conserved transmembrane protein; Involved in the biosynthesis of decaprenylphosphoryl arabinose (DPA) a precursor for arabinan synthesis in mycobacterial cell wall biosynthesis. Could be involved in the dephosphorylation of decaprenylphosphoryl-5-phosphoribose (DPPR) to decaprenyl-phospho- ribose (DPR) (By similarity). (165 aa) |
| | ubiA | Decaprenyl-phosphate phosphoribosyltransferase; Involved in the biosynthesis of decaprenylphosphoryl arabinose (DPA) a precursor for arabinan synthesis in mycobacterial cell wall biosynthesis. Catalyzes the transfer of a 5-phosphoribosyl residue from phosphoribose diphosphate (pRpp) to decaprenyl phosphate (DP) to form decaprenylphosphoryl-5-phosphoribose (DPPR). The enzyme favors polyprenyl phosphate with 50-60 carbon atoms uses C-75 polyprenyl phosphate less efficiently than C-50 or C-60. Belongs to the UbiA prenyltransferase family. (302 aa) |
| | aftB | Possible arabinofuranosyltransferase AftB; Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the transfer of arabinofuranosyl (Araf) residues from the sugar donor decaprenyl-phospho-arabinose (DPA) to the arabinan domain to form terminal beta-(1->2)-linked Araf residues, which marks the end point for AG arabinan biosynthesis before decoration with mycolic acids. (627 aa) |
| | aftA | Arabinofuranosyltransferase AftA; Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the addition of the first key arabinofuranosyl (Araf) residue from the sugar donor decaprenyl-phospho-arabinose (DPA) on the C-5 of a 6-linked galactofuranosyl (Galf) of the galactan domain, thus 'priming' the galactan for further elaboration by other arabinofuranosyltransferases. It is not able to add an Araf residue to a terminal Galf. Belongs to the glycosyltransf [...] (643 aa) |
| | dprE2 | Decaprenylphosphoryl-D-2-keto erythro pentose reductase; Component of the DprE1-DprE2 complex that catalyzes the 2- step epimerization of decaprenyl-phospho-ribose (DPR) to decaprenyl- phospho-arabinose (DPA), a key precursor that serves as the arabinose donor required for the synthesis of cell-wall arabinans. DprE1 catalyzes the first step of epimerization, namely FAD-dependent oxidation of the C2' hydroxyl of DPR to yield the keto intermediate decaprenyl-phospho-2'-keto-D- arabinose (DPX). The intermediate DPX is then transferred to DprE2 subunit of the epimerase complex, most probab [...] (254 aa) |
| | dprE1 | Decaprenylphosphoryl-beta-D-ribose 2'-oxidase; Component of the DprE1-DprE2 complex that catalyzes the 2- step epimerization of decaprenyl-phospho-ribose (DPR) to decaprenyl- phospho-arabinose (DPA), a key precursor that serves as the arabinose donor required for the synthesis of cell-wall arabinans. DprE1 catalyzes the first step of epimerization, namely FAD-dependent oxidation of the C2' hydroxyl of DPR to yield the keto intermediate decaprenyl-phospho-2'-keto-D- arabinose (DPX). The intermediate DPX is then transferred to DprE2 subunit of the epimerase complex, most probably through [...] (461 aa) |
| | Rv3789 | GTRA family protein; Required for arabinosylation of arabinogalactan (AG), an essential component of the mycobacterial cell wall. Probably acts as an anchor protein recruiting AftA, the first arabinosyl transferase involved in AG biosynthesis; Belongs to the GtrA family. (121 aa) |
| | glfT1 | UDP-galactofuranosyl transferase GlfT1; Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the transfer of the first two galactofuranosyl (Galf) units from UDP- galactofuranose (UDP-Galf) onto the rhamnosyl-GlcNAc-diphospho- decaprenol (Rha-GlcNAc-PP-C50) acceptor, yielding galactofuranosyl- galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha- GlcNAc-PP-C50). Thus, GlfT1 is the initiator of galactan synthesis, while GlfT2 continues [...] (304 aa) |
| | Rv3740c | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Upon expression in E.coli has a weak triacylglycerol synthase function, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions weakly as a wax synthase, as it incorporates palmityl alcohol into wax esters in the presence of palmitoyl-CoA. (448 aa) |
| | tgs2 | Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs2; Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (454 aa) |
| | glpK | Probable glycerol kinase GlpK (ATP:glycerol 3-phosphotransferase) (glycerokinase) (GK); Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn- glycerol 3-phosphate; Belongs to the FGGY kinase family. (517 aa) |
| | otsB2 | Trehalose 6-phosphate phosphatase OtsB2 (trehalose-phosphatase) (TPP); Removes the phosphate from trehalose 6-phosphate to produce free trehalose. (391 aa) |
| | Rv3401 | Conserved protein; Rv3401, (MTCY78.27c), len: 786 aa. Conserved protein, may be an hydrolase or a transferase, equivalent to Q49736|ML0392|B1620_F1_30 hypothetical 88.1 KDA protein from Mycobacterium leprae (792 aa), FASTA scores: opt: 4820, E(): 0, (91.45% identity in 782 aa overlap). Also highly similar to Q9L2I8|SCF42.31c putative glycosyl transferase from Streptomyces coelicolor (792 aa), FASTA scores: opt: 3060, E(): 2.9e-179, (59.25% identity in 785 aa overlap); and similar to others e.g. Q9K109|NMB0390 maltose phosphorylase from Neisseria meningitidis (serogroup B) (752 aa), FAS [...] (786 aa) |
| | Rv3404c | Conserved hypothetical protein; Sugar N-formyltransferase that catalyzes the conversion of dTDP-4-amino-4,6-dideoxyglucose into dTDP-4-formamido-4,6- dideoxyglucose using N(10)-formyltetrahydrofolate as the carbon source. Plays a role in virulence. Has no activity on dTDP-3-amino-3,6-dideoxyglucose, dTDP-3-amino-3,6- dideoxygalactose, UDP-4-amino-4-deoxyarabinose, and GDP-4-amino-4,6- dideoxymannose. (234 aa) |
| | glmS | Glutamine--fructose-6-phosphate aminotransferase [isomerizing]; Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source. (624 aa) |
| | mrsA | Probable phospho-sugar mutase / MrsA protein homolog; Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate. (448 aa) |
| | rmlB | dTDP-glucose 4,6-dehydratase RmlB; Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6- deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D- N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage. (331 aa) |
| | rmlC | dTDP-4-dehydrorhamnose 3,5-epimerase; Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage. (202 aa) |
| | Rv3480c | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Upon expression in E.coli has a weak triacylglycerol synthase function, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions weakly as a wax synthase, as it incorporates palmityl alcohol into wax esters in the presence of palmitoyl-CoA. (497 aa) |
| | otsA | Trehalose-6-phosphate synthase; Involved in the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1- phosphate (M1P), and probably in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to D-glucose 6- phosphate (Glc-6-P) to form trehalose-6-phosphate. Is specific for the glucosyl acceptor (Glc-6-P cannot be replaced by either mannose-6-P, fructose-6-P or glucosamine-6-P), but any of the glucose sugar nucleotides can be used as glucosyl donors. It is more active with th [...] (500 aa) |
| | galE1 | UDP-glucose 4-epimerase; Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD (By similarity). (314 aa) |
| | Rv3683 | Conserved protein; Rv3683, (MTV025.031), len: 319 aa. Conserved protein, equivalent to Q9CB84|ML2309 hypothetical protein from Mycobacterium leprae (330 aa) FASTA scores: opt: 1791,E(): 9e-107, (85.45% identity in 296 aa overlap). Also similar to Q9X935|SCH66.03 conserved hypothetical protein from Streptomyces coelicolor (309 aa) FASTA scores: opt: 610, E(): 1.4e-31, (51.45% identity in 307 aa overlap); and Q9RRY7|YN45_DEIRA|DR2345 hypothetical protein from Deinococcus radiodurans (305 aa) FASTA scores: opt: 243,E(): 3.2e-08, (31.1% identity in 315 aa overlap) and some similarity to ot [...] (319 aa) |
| | Rv3371 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (446 aa) |
| | icd1 | Isocitrate dehydrogenase [NADP]; Rv3339c, (MTV016.39c), len: 409 aa. Probable icd1,isocitrate dehydrogenase NADP-dependent, highly similar to many e.g. Q9A5C8|CC2522 from Caulobacter crescentus (403 aa), FASTA scores: opt: 1972, E(): 4.6e-115, (72.45% identity in 403 aa overlap); AAF73472|ICD from Rhizobium meliloti (404 aa), FASTA scores: opt: 1968, E(): 8.1e-115,(73.2% identity in 403 aa overlap); P50215|IDH_SPHYA from Sphingomonas yanoikuyae (406 aa), FASTA scores: opt: 1964,E(): 1.4e-114, (71.45% identity in 403 aa overlap); etc. Contains PS00470 Isocitrate and isopropylmalate dehy [...] (409 aa) |
| | nagA | Rv3332, (MTV016.32), len: 383 aa. Probable nagA,N-acetylglucosamine-6-phosphate deacetylase, similar to many e.g. Q9KXV7|SCD95A.17c putative deacetylase from Streptomyces coelicolor (381 aa), FASTA scores: opt: 1090,E(): 1.6e-55, (47.8% identity in 385 aa overlap); Q9PDB4|XF1465 N-acetylglucosamine-6-phosphate deacetylase from Xylella fastidiosa (386 aa), FASTA scores: opt: 667,E(): 3.5e-31, (38.3% identity in 394 aa overlap); Q9AAZ9|CC0443 N-acetylglucosamine-6-phosphate deacetylase from Caulobacter crescentus (378 aa), FASTA scores: opt: 661, E(): 7.5e-31, (38.9% identity in 383 aa o [...] (383 aa) |
| | pmmB | Rv3308, (MTV016.07), len: 534 aa. Probable pmmB,phosphomannomutase, equivalent to Q9CCL7|PMMB|ML0706 putative phospho-sugar mutase from Mycobacterium leprae (538 aa), FASTA scores: opt: 2681, E(): 1.4e-150, (76.95% identity in 538 aa overlap). Also similar to others e.g. Q9AD82|SCK13.08c from Streptomyces coelicolor (549 aa),FASTA scores: opt: 1378, E(): 8.9e-74, (46.7% identity in 529 aa overlap); Q9ZHL4|PMM (fragment so no homology at N-terminus for this one) from Haemophilus ducreyi (443 aa),FASTA scores: opt: 935, E(): 9.6e-48, (39.4% identity in 449 aa overlap); P18159|YHXB_BACSU [...] (534 aa) |
| | acpA | Probable acyl carrier protein AcpA (ACP); Rv0033, (MTCY10H4.33), len: 87 aa. Probable acpA (alternate gene name: acpP), acyl carrier protein, similar to others. Also similar to proteins of Mycobacterium tuberculosis Rv1344 and Rv2244 (31.5% identity in 73 aa overlap). (87 aa) |
| | ino1 | Inositol-3-phosphate synthase; Catalyzes the conversion of glucose 6-phosphate to 1D-myo- inositol 3-phosphate; Belongs to the myo-inositol 1-phosphate synthase family. (367 aa) |
| | celA1 | Glucanase; Rv0062, (MTV030.05), len: 380 aa. Possible celA1,cellulase, similar to many. Seems to belong to cellulase family B (family 6 of glycosyl hydrolases). Note that previously known as celA. (380 aa) |
| | icd2 | Isocitrate dehydrogenase [NADP]; Rv0066c, (MTV030.09c), len: 745 aa. Probable icd2,isocitrate dehydrogenase NADP-dependent. Belongs to the monomeric-type family of IDH. Note that in H37Rv, Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2. (745 aa) |
| | sdaA | Rv0069c, (MTV030.12c), len: 461 aa. Probable sdaA,L-serine dehydratase. Belongs to the iron-sulfur dependent L-serine dehydratase family. Cofactor: iron-sulfur (4FE-4S) (probable). (461 aa) |
| | Rv0111 | Rv0111, (MTV031.05), len: 685 aa. Possible transmembrane acyltransferase, equivalent to AA22904.1|AL035300 putative acyltransferase from Mycobacterium leprae (696 aa). Also similar to others e.g. C69975 acyltransferase homolog yrhL from Bacillus subtilis (634 aa), FASTA scores: opt: 520, E(): 4e-22, (36.4% identity in 382 aa overlap). Very similar to Mycobacterium tuberculosis proteins Rv0228, Rv1254, Rv1565c, etc. (685 aa) |
| | gmhA | Probable sedoheptulose-7-phosphate isomerase GmhA (phosphoheptose isomerase); Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate; Belongs to the SIS family. GmhA subfamily. (196 aa) |
| | gmhB | D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase; Converts the D-glycero-alpha-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-alpha-D-manno-heptose 1-phosphate by removing the phosphate group at the C-7 position. (190 aa) |
| | hddA | Rv0115, (MTV031.09), len: 386 aa. Possible hddA,D-alpha-D-heptose-7-phosphate kinase (see citation below),similar to several hypothetical proteins and sugar kinases e.g. AAK27850.1|AF324836_3 D-glycero-D-manno-heptose 7-phosphate kinase from Aneurinibacillus thermoaerophilus (341 aa); AAK80995.1|AE007802_11 Sugar kinase from Clostridium acetobutylicum (364 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (386 aa) |
| | treS | Trehalose synthase TreS; Catalyzes the reversible interconversion of maltose and trehalose by transglucosylation. Also displays amylase activity, catalyzing the endohydrolysis of (1->4)-alpha-D- glucosidic linkages in glycogen and maltooligosaccharides such as maltoheptaose, to produce maltose which then can be converted to trehalose. TreS plays a key role in the utilization of trehalose for the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P). Might also function as a sensor and/or regulator of trehalose levels [...] (601 aa) |
| | mak | Maltokinase Mak; Catalyzes the ATP-dependent phosphorylation of maltose to maltose 1-phosphate (By similarity). Is involved in a branched alpha- glucan biosynthetic pathway from trehalose, together with TreS, GlgE and GlgB; Belongs to the aminoglycoside phosphotransferase family. (455 aa) |
| | bglS | Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase); Rv0186, (MTCI28.25b), len: 691 aa. Probable bglS,beta-glucosidase, highly similar to many e.g. BGLS_AGRTU|P27034 beta-glucosidase from Agrobacterium tumefaciens (818 aa), FASTA scores: opt: 643, E(): 0,(32.5% identity in 842 aa overlap). Seems to belong to family 3 of glycosyl hydrolases. (691 aa) |
| | pckA | Phosphoenolpyruvate carboxykinase [GTP]; Involved in the gluconeogenesis, in growth on fatty acids and is important for initiation of infection in the macrophages. Catalyzes the GTP-dependent conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle; Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. (606 aa) |
| | Rv0221 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (469 aa) |
| | Rv0228 | Rv0228, (MTCY08D5.23), len: 407 aa. Probable integral membrane acyltransferase, equivalent to 3063875|CAA18555.1|AL022486|T44870 acyltransferase from Mycobacterium leprae (384 aa), FASTA scores: opt: 2004,E(): 0, (79.3% identity in 381 aa overlap). Also similar to others e.g. Q11064 probable acyltransferase CY50.28C (383 aa), FASTA scores: opt: 372, E(): 2.6e-16, (35.9% identity in 359 aa overlap); Q00718|MDMB_STRMY acyltransferase. Very similar to Rv0111, Rv1254, etc from Mycobacterium tuberculosis. (407 aa) |
| | aftD | Possible arabinofuranosyltransferase AftD; Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the addition of an arabinofuranosyl (Araf) residue from the sugar donor decaprenyl-phospho-arabinose (DPA) on the C-3 of an alpha-(1->5)-linked Araf from the arabinan backbone of AG. (1400 aa) |
| | lpqI | Probable conserved lipoprotein LpqI; Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptidoglycan fragments. Acts as a regulator for GlcNAc-MurNAc levels by cleaving disaccharides and allowing the breakdown of MurNAc. (388 aa) |
| | stf0 | Conserved protein; Catalyzes the sulfuryl group transfer from 3'- phosphoadenosine-5'-phosphosulfate (PAPS) to trehalose, leading to trehalose-2-sulfate (T2S). The sulfation of trehalose is the first step in the biosynthesis of sulfolipid-1 (SL-1), a major cell wall glycolipid and the most abundant sulfated metabolite found in Mycobacterium tuberculosis, that is a potential virulence factor thought to mediate host-pathogen interactions. (267 aa) |
| | Rv0315 | Rv0315, (MTCY63.20), len: 294 aa. Possible beta-1,3-glucanase precursor (has hydrophobic stretch in its N-terminal part), similar to others e.g. Q51333|AAC44371.1 beta-1,3-glucanase II a from Oerskovia xanthineolytica (306 aa), FASTA scores: opt: 76, E(): 3e-14, (34.1% identity in 302 aa overlap); and AAC38290.1|AF052745 beta-1,3-glucanase II from Oerskovia xanthineolytica (435 aa). Contains glycosyl hydrolases family 16 active site signature (PS01034). (294 aa) |
| | udgA | Probable UDP-glucose 6-dehydrogenase UdgA (UDP-GLC dehydrogenase) (UDP-GLCDH) (UDPGDH); Rv0322, (MTCY63.27), len: 443 aa. Probable udg (alternate gene name: rkpK), UDP-glucose 6-dehydrogenase, highly similar to others e.g. CAC44517.1|AL596138 putative UDP-glucose 6-dehydrogenase from Streptomyces coelicolor (447 aa); Q56812 UDP-glucose dehydrogenase from Xanthomonas campestris (445 aa), FASTA scores: opt: 713, E(): 0, (41.9% identity in 351 aa overlap); etc. Also similar to several GDP-mannose 6-dehydrogenase. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UDP-g [...] (443 aa) |
| | rmlA | Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage; Belongs to the glucose-1-phosphate thymidylyltransferase family. (288 aa) |
| | fba | Probable fructose-bisphosphate aldolase Fba; Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. (344 aa) |
| | Rv0365c | Conserved protein; Rv0365c, (MTCY13E10.27c), len: 376 aa (start uncertain). Conserved protein (see citation below), very similar to G388212|CAA35191.1, a truncated ORF immediately upstream of the Corynebacterium glutamicum fda gene encoding fructose-1,6-biphosphate aldolase (304 aa), FASTA scores: E(): 7.1e-19, (42.2% identity in 296 aa overlap). (376 aa) |
| | fgd1 | F420-dependent glucose-6-phosphate dehydrogenase Fgd1; Catalyzes the coenzyme F420-dependent oxidation of glucose 6- phosphate to 6-phosphogluconolactone. Appears to have a role in resistance to oxidative stress, via its consumption of G6P that serves as a source of reducing power to combat oxidative stress in mycobacteria. More precisely, is likely involved in a F420-dependent anti-oxidant mechanism that protects M.tuberculosis against oxidative stress and bactericidal agents. (336 aa) |
| | lpdC | Dihydrolipoyl dehydrogenase; Lipoamide dehydrogenase is an essential component of the alpha-ketoacid dehydrogenase complexes, namely the pyruvate dehydrogenase (PDH) complex, the branched-chain alpha-ketoacid dehydrogenase (BCKADH) complex, and likely also the 2-oxoglutarate dehydrogenase (ODH) complex. Catalyzes the reoxidation of dihydrolipoyl groups which are covalently attached to the lipoate acyltransferase components (E2) of the complexes. Is also able to catalyze the transhydrogenation of NADH and thio-NAD(+) in the absence of D,L- lipoamide, and the NADH-dependent reduction of [...] (464 aa) |
| | icl1 | Isocitrate lyase Icl (isocitrase) (isocitratase); Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It could also catalyze the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route) (By similarity). (428 aa) |
| | deoC | Probable deoxyribose-phosphate aldolase DeoC (phosphodeoxyriboaldolase) (deoxyriboaldolase); Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5- phosphate. (224 aa) |
| | gpm1 | Probable phosphoglycerate mutase 1 Gpm1 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM); Catalyzes the interconversion of 2-phosphoglycerate and 3- phosphoglycerate; Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. (249 aa) |
| | Rv0517 | Rv0517, (MTCY20G10.07), len: 436 aa. Possible acyltransferase, integral membrane protein, equivalent (but longer 26 aa in N-terminus) to AAK44761.1|AE006954 putative acyltransferase from Mycobacterium tuberculosis strain CDC1551 (410 aa). Also similar to many acyltransferases e.g. MDMB_STRMY|Q00718 from Streptomyces mycarofaciens (387 aa), FASTA scores: opt: 200, E(): 1.1e-08, (28.2% identity in 394 aa overlap). And similar to Rv0111, Rv0228, Rv1254,Rv1565c from Mycobacterium tuberculosis. (436 aa) |
| | gpdA1 | Probable glycerol-3-phosphate dehydrogenase 2 [NAD(P)+]; Rv0564c, (MTV039.02c), len: 341 aa. Possible gpdA1(alternate gene names: gpsA, glyC),glycerol-3-phosphate dehydrogenase [NAD(P)+] dependent,similar to many other glycerol-3-phosphate dehydrogenases e.g. P46919|GPDA_BACSU from Bacillus subtilis (345 aa),FASTA scores: opt: 731, E(): 0, (37.3% identity in 332 aa overlap); etc. Also similar to Rv2982c|gpdA2|MTCY349.05|Z83018|MTCY349_5 from Mycobacterium tuberculosis (334 aa), FASTA scores: opt: 740, E(): 0, (40.4% identity in 322 aa overlap). Contains PS00017 ATP/GTP-binding site mot [...] (341 aa) |
| | Rv0584 | Rv0584, (MTV039.22), len: 877 aa. Possible conserved exported protein, similar to other hypothetical proteins which are not necessarily secreted e.g. CAB61925.1|AL133278 putative secreted protein from Streptomyces coelicolor (772 aa); AAD51075.1|AF175722_1|AF175722 immunoreactive 89kD antigen PG87 from Porphyromonas gingivalis (781 aa), FASTA scores: opt: 637, E(): 2.1e-30, (29.1% identity in 794 aa overlap); etc. Contains PS00699 Nitrogenases component 1 alpha and beta subunits signature 1. Has potential N-terminal signal peptide. Predicted to be an outer membrane protein (See Song et [...] (877 aa) |
| | galTa | Rv0618, (MTCY19H5.03c), len: 231 aa (probable partial CDS). Probable galTa, first part of galactose-1-phosphate uridylyltransferase, highly similar to N-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 296, E(): 1.4e-11, (50.8% identity in 177 aa overlap); etc. Also highly similar to N-terminal half of some UDP glucose--hexose-1-phosphate uridylyltransferases. N-terminal 28 aa similar to MTCY20H11.08|Rv0627|MTCY20H11.08 conserved hypothetical protein from Mycobacterium tuberculo [...] (231 aa) |
| | galTb | Rv0619, (MTCY19H5.02c), len: 181 aa (probable partial CDS). Probable galTb, second part of galactose-1-phosphate uridylyltransferase, highly similar to C-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 416, E(): 5.2e-22, (43.0% identity in 186 aa overlap), etc. Cosmid sequence is correct but there may be a frameshift mutation in this region which would allow the two ORFS to be joined. Belongs to the galactose-1-phosphate uridylyltransferase family 1. Note that previously known [...] (181 aa) |
| | galK | Probable galactokinase GalK (galactose kinase); Catalyzes the transfer of the gamma-phosphate of ATP to D- galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). (363 aa) |
| | Rv0648 | Alpha-mannosidase; Rv0648, (MTCY20H10.29), len: 1215 aa. Alpha-mannosidase (see citation below), showing some similarity to hypothetical proteins and various sugar hydrolases e.g. SYCSLRA_6|Q55528 hypothetical 1 20.4 kDa protein from Synechocystis (1042 aa), FASTA scores: opt: 260, E(): 3.6e-08, (23.4% identity in 602 aa overlap); etc. Contains PS00659 Glycosyl hydrolases family 5 signature. (1215 aa) |
| | fucA | Rv0727c, (MTV41.01c, MTCY210.46c), len: 218 aa. Possible fucA, L-fuculose-1-phosphate aldolase, similar to many e.g. NP_386339.1|NC_003047 putative L-fuculose phosphate aldolase protein from Sinorhizobium meliloti (222 aa); P11550|FUCA_ECOLI L-fuculose phosphate aldolase from Escherichia strain K12 (215 aa), FASTA scores: opt: 372,E(): 4.1e-19, (34.6% identity in 185 aa overlap); etc. Belongs to the aldolase class II family, ARAD/FUCA subfamily. Cofactor: binds one zinc ion per molecule. (218 aa) |
| | xylB | Rv0729, (MTV041.03), len: 448 aa. Possible xylB,D-xylulose-kinase (xylulokinase). C-terminus highly similar to AAD09880.1|U77912 unknown protein from Mycobacterium bovis (102 aa); and N-terminus highly similar to T45387|Z98756|MLCB2492_25 hypothetical protein from Mycobacterium leprae (110 aa), FASTA scores: opt: 427, E(): 1.1e-19, (60.9% identity in 110 aa overlap). Also similar to xylA/xylB genes from various bacterial species e.g. AAC26499.1|AF045245 D-xylulose-kinase from Klebsiella pneumoniae (487 aa); NP_418021.1|NC_000913 xylulokinase from Escherichia coli strain K12 (484 aa), F [...] (448 aa) |
| | PE_PGRS11 | PE-PGRS family protein PE_PGRS11; Induces maturation and activation of human dendritic cells (DCs), via TLR2-dependent activation of ERK1/2, p38 MAPK, and NF-kappa- B signaling pathways, and enhances the ability of DCs to stimulate CD4(+) T cells. By activating DCs, could potentially contribute to the initiation of innate immune responses during tuberculosis infection and hence regulate the clinical course of tuberculosis. Involved in resistance to oxidative stress, via TLR2-dependent activation of the PI3K-ERK1/2-NF-kappa-B signaling pathway and expression of COX-2 and Bcl2. Also abol [...] (584 aa) |
| | Rv0784 | Rv0784, (MTC369.28), len: 228 aa. Conserved hypothetical protein, with some similarity to MLCB5_20|O05752 hypothetical protein from Mycobacterium leprae (193 aa), FASTA scores: opt: 141, E(): 0.0022,(36.0% identity in 114 aa overlap). Also similar to N-terminus of NP_253002.1|NC_002516 conserved hypothetical protein from Pseudomonas aeruginosa (253 aa). (228 aa) |
| | cpsY | Exopolysaccharide phosphotransferase CpsY; Rv0806c, (MTCY07H7A.03), len: 532 aa. Possible cpsY,UDP-glucose-4-epimerase, equivalent to Q50025|CPSY probable UDP-glucose-4-epimerase from Mycobacterium leprae (542 aa),FASTA scores: opt: 2964, E(): 0, (82.3% identity in 530 aa overlap). Also similar to AAC38286.1|AF019760|SACB CpsY homolog (involved in meningococcal capsule biosynthesis) from Neisseria meningitidis serogroup a (545 aa); Q51151 capsule gene complex UPD-glucose-4-epimerase (gale) from Neisseria meningitidis (373 aa), FASTA scores: opt: 496,E(): 9.5e-27, (29.3% identity in 358 [...] (532 aa) |
| | citA | Probable citrate synthase II CitA; Rv0889c, (MTCY31.17c), len: 373 aa. Probable citA (alternate gene name: gltA), citrate synthase 2, highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30,(33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. (373 aa) |
| | Rv0895 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (505 aa) |
| | pgi | Glucose-6-phosphate isomerase; Rv0946c, (MTCY10D7.28), len: 553 aa. Probable pgi,glucose-6-phosphate isomerase, equivalent to NP_301236.1|NC_002677 glucose-6-phosphate isomerase from Mycobacterium leprae (554 aa); and P96803|G6PI_MYCSM glucose-6-phosphate isomerase from Mycobacterium smegmatis (442 aa). Also highly similar to others e.g. T36015 glucose-6-phosphate isomerase from Streptomyces coelicolor (551 aa); P11537|G6PI_ECOLI|GPI glucose-6-phosphate isomerase from Escherichia coli strains K12 and O157:H7 (549 aa), FASTA scores: opt: 1779, E(): 0, (51.4% identity in 554 aa overlap); [...] (553 aa) |
| | prsA | Ribose-phosphate pyrophosphokinase; Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) and of the decaprenylphosphoryl-arabinose (DPA), an essential precursor for the mycobacterial cell wall biosynthesis. Catalyzes the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P) to yield phosphoribosyl diphosphate (PRPP) and AMP. It can also use GTP, CTP and UTP as diphosphoryl donors. (326 aa) |
| | glmU | Probable UDP-N-acetylglucosamine pyrophosphorylase GlmU; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain. (495 aa) |
| | eno | Probable enolase Eno; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis; Belongs to the enolase family. (429 aa) |
| | Rv1084 | Conserved protein; Rv1084, (MTV017.37), len: 673 aa. Conserved protein,similar to P37512|YYAL_BACSU hypothetical protein from Bacillus subtilis (689 aa), FASTA scores: opt: 1063, E() : 0, (36.5% identity in 696 aa overlap); AE0009|AE000983_10 Archaeoglobus fulgidus section 1 (642 aa), FASTA scores: opt: 1018, E(): 0, (37.2% identity in 600 aa overlap). Also similar to AE001938|AE001938_9 Deinococcus radiodurans (690 aa), FASTA scores: opt: 1097, E(): 0, (41.6% identity in 694 aa overlap). (673 aa) |
| | celA2b | Rv1090, (MTV017.43), len: 151 aa. Probable celA2b,second part of cellulase (endoglucanase), similar to C-terminus of others e.g. O08468 cellulase CEL2 from Streptomyces halstedi (377 aa), FASTA scores: opt: 554,E(): 1.2e-30, (52.0% identity in 152 aa overlap); etc. Gene appears to have been inactivated by frameshift mutations but no errors could be found that would account for this. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007); Belongs to the glycosyl hydrolase 12 (cellulase H) family. (151 aa) |
| | Rv1096 | Rv1096, (MTV017.49), len: 291 aa. Possible glycosyl hydrolase, possibly deacetylase or esterase. Equivalent to AL049491|MLCB1222_13 Mycobacterium leprae (291 aa) (81.3% identity in 289 aa overlap). Similar at the C-terminus to enzymes involved in carbohydrate degradation including Z99110|BSUB0007_92 endo-1,4-beta-xylanase homolog yjeA from Bacillus subtilis (467 aa), FASTA scores: opt: 418, E(): 2.6e-17, (38.6% identity in 184 aa overlap); M64552|STMXLNB_2 acetyl-xylan esterase from Streptomyces lividans (335 aa), FASTA scores: opt: 371, E(): 1.1e-14,(31.6% identity in 237 aa overlap); [...] (291 aa) |
| | glpX | Fructose 1,6-bisphosphatase GlpX; Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate. Seems to be the major FBPase of M.tuberculosis and to play a key role in gluconeogenesis for conversion of lipid carbon into cell wall glycans. Does not display activity against inositol 1-phosphate. (362 aa) |
| | zwf1 | Probable glucose-6-phosphate 1-dehydrogenase Zwf1 (G6PD); Catalyzes the oxidation of glucose 6-phosphate to 6- phosphogluconolactone. (466 aa) |
| | gnd2 | Rv1122, (MTCY22G8.11), len: 340 aa. Probable gnd2,6-phosphogluconate dehydrogenase, decarboxylating, highly similar to Q53917 6-phosphogluconate dehydrogenase from Streptomyces coelicolor (291 aa), fasta scores: opt: 431,E(): 2.2e-20, (44.5% identity in 335 aa overlap). Also similar to Rv1844c|MTCY359.29|gnd1 probable 6-phosphogluconate dehydrogenase from Mycobacterium tuberculosis (485 aa), FASTA score: (33.0% identity in 351 aa overlap). Note that Rv1844c|MTCY359.29|gnd1 is most similar to gnd's from Gram negative organisms, while gnd2 is most similar to gnd's from Gram positive orga [...] (340 aa) |
| | prpC | Probable methylcitrate synthase PrpC; Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the Claisen condensation of propionyl-CoA and oxaloacetate (OAA) to yield 2-methylcitrate (2-MC) and CoA. Also catalyzes the condensation of oxaloacetate with acetyl-CoA. (393 aa) |
| | papA3 | Probable conserved polyketide synthase associated protein PapA3; Involved in the biosynthesis of polyacyltrehalose (PAT) which could have a role in anchoring the bacterial capsule. In vitro catalyzes the sequential transfer of two palmitoyl groups onto a single glucose residue of trehalose generating the diacylated product 2,3- diacyltrehalose (trehalose dipalmitate). Although palmitoyl-CoA (PCoA) seems to be the physiological acyl donor, PapA3 can also use docosanoyl (22-carbon saturated fatty acid) coenzyme A as acyl donor. (472 aa) |
| | glgA | Putative glycosyl transferase GlgA; Involved in the biosynthesis of the maltose-1-phosphate (M1P) building block required for alpha-glucan production by the key enzyme GlgE. Catalyzes the formation of an alpha-1,4 linkage between glucose from ADP-glucose and glucose 1- phosphate (G1P) to yield maltose-1-phosphate (M1P). Also able to catalyze the elongation of the non-reducing ends of glycogen, maltodextrin and maltoheptaose using ADP-glucose as sugar donor, however the rate of the reaction appears to be too low to be physiologically relevant. GlgM is also able to use UDP-glucose as sug [...] (387 aa) |
| | glgC | Glucose-1-phosphate adenylyltransferase GlgC (ADP-glucose synthase) (ADP-glucose pyrophosphorylase); Involved in the biosynthesis of ADP-glucose building block required in the biosynthesis of maltose-1-phosphate (M1P) and in the elongation reactions to produce linear alpha-1,4-glucans. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc; Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family. (404 aa) |
| | mdh | Probable malate dehydrogenase Mdh; Catalyzes the reversible oxidation of malate to oxaloacetate. Belongs to the LDH/MDH superfamily. MDH type 2 family. (329 aa) |
| | Rv1254 | Rv1254, (MTCY50.28c), len: 383 aa. Probable Acyltransferase, similar to G927228 midecamycin 4-0-propionyl transferase (fragment) (388 aa), FASTA scores, opt: 305, E(): 5.6e-14, (28.4% identity in 377 aa overlap). Also similar to other Mycobacterium tuberculosis acyltransferases e.g. Rv0111, Rv0228, etc. Contains PS00881 Protein splicing signature. (383 aa) |
| | rfe | Decaprenyl-phosphate N-acetylglucosaminephosphotransferase; Involved in the biosynthesis of the disaccharide D-N- acetylglucosamine-L-rhamnose which plays an important role in the mycobacterial cell wall as a linker connecting arabinogalactan and peptidoglycan via a phosphodiester linkage. Catalyzes the transfer of the N-acetylglucosamine-1-phosphate (GlcNAc-1P) moiety from UDP-GlcNAc onto the carrier lipid decaprenyl phosphate (C50-P), yielding GlcNAc- pyrophosphoryl-decaprenyl (GlcNAc-PP-C50). (404 aa) |
| | glgB | 1,4-alpha-glucan branching enzyme GlgB (glycogen branching enzyme); Essential enzyme that catalyzes the formation of the alpha- 1,6-glucosidic linkages in glucan chains by scission of a 1,4-alpha- linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. Is involved in the biosynthesis of both glycogen and capsular alpha-D- glucan. (731 aa) |
| | glgE | Probable glucanase GlgE; Essential maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)- glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP5 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Exhibits an alpha-retaining catalytic mechanism. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen in the presence [...] (701 aa) |
| | glgP | Probable glycogen phosphorylase GlgP; Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity). (863 aa) |
| | rpe | Probable ribulose-phosphate 3-epimerase Rpe (PPE) (R5P3E) (pentose-5-phosphate 3-epimerase); Catalyzes the reversible epimerization of D-ribulose 5- phosphate to D-xylulose 5-phosphate; Belongs to the ribulose-phosphate 3-epimerase family. (232 aa) |
| | Rv1425 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (459 aa) |
| | gap | Probable glyceraldehyde 3-phosphate dehydrogenase Gap (GAPDH); Catalyzes the oxidative phosphorylation of glyceraldehyde 3- phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG; Belongs to the glyceraldehyde-3-ph [...] (339 aa) |
| | pgk | Rv1437, (MTCY493.17c), len: 412 aa. Probable pgk,Phosphoglycerate kinase, highly similar to many e.g. PGK_MYCLE|P46712 Mycobacterium leprae (416 aa), FASTA scores: opt: 2153, E(): 0, (80.4% identity in 414 aa overlap). Contains PS00111 Phosphoglycerate kinase signature. Belongs to the phosphoglycerate kinase family. (412 aa) |
| | tpi | Probable triosephosphate isomerase Tpi (TIM); Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D- glyceraldehyde-3-phosphate (G3P); Belongs to the triosephosphate isomerase family. (261 aa) |
| | devB | Probable 6-phosphogluconolactonase DevB (6PGL); Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate. (247 aa) |
| | zwf2 | Probable glucose-6-phosphate 1-dehydrogenase Zwf2 (G6PD); Catalyzes the oxidation of glucose 6-phosphate to 6- phosphogluconolactone. (514 aa) |
| | tal | Probable transaldolase Tal; Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway; Belongs to the transaldolase family. Type 2 subfamily. (373 aa) |
| | Rv1503c | Rv1503c, (MTCY277.25c), len: 182 aa. Conserved hypothetical protein, similar to C-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 565,E(): 0, (49.4% identity in 170 aa overlap); Rv1503c and Rv1504c are both similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene. (182 aa) |
| | Rv1504c | Rv1504c, (MTCY277.26c), len: 199 aa. Conserved hypothetical protein, similar to N-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 863,E(): 0, (68.0% identity in 194 aa overlap); Rv1503c and Rv1504c are similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene. (199 aa) |
| | Rv1524 | Rv1524, (MTCY19G5.04c), len: 414 aa. Probable glycosyltransferase, similar to many e.g. P96559|U84349 glycosyltransferase GTFB from Amycolatopsis orientalis (407 aa), FASTA scores: opt: 363, E(): 6.2e-23, (28.8% identity in 430 aa overlap); also high similarity to Rv1526c|MTCY19G5.02 Mycobacterium tuberculosis hypothetical protein (58.7% identity in 416 aa overlap); and AF143772|AF143772_15 glycosyltransferase gtfB from Mycobacterium avium strain 215 (418 aa), FASTA scores: opt: 1801, E(): 0, (65.2% identity in 417 aa overlap). (414 aa) |
| | Rv1526c | Rv1526c, (MTCY19G5.02), len: 426 aa. Probable glycosyltransferase, highly similar to G467196 Protein L518_C2_147 from Mycobacterium leprae (421 aa), FASTA scores, opt: 1497, E(): 0, (55.0% identity in 424 aa overlap); similar to G452504 rhamnosyltransferase (24.7% identity in 433 aa overlap); and P96565|U84350 glycosyltransferase GTFE from Amycolatopsis orientalis (408 aa), E(): 3.4e-24, (28.4% identity in 429 aa overlap), also high similarity to Rv1524|MTCY19G5.04c (58.7 % identity in 416 aa overlap). (426 aa) |
| | treZ | Maltooligosyltrehalose trehalohydrolase TreZ; Is involved in the biosynthesis of trehalose but not in that of capsular glucan and glycogen. (580 aa) |
| | treY | Maltooligosyltrehalose synthase TreY; Catalyzes the conversion of maltooligosaccharide into the non-reducing saccharide, maltooligosyl trehalose (alpha-maltooligosyl alpha-D-glucoside) by intramolecular transglycosylation. (765 aa) |
| | treX | Rv1564c, (MTCY48.01), len: 721 aa. Probable treX (previously called glgX), Maltooligosyltrehalose synthase. Strong similarity to D83245|g1890053 treX, glycogen debranching enzyme (glgX) from Sulfolobus acidocaldarius (713 aa), FASTA score: opt: 2396, E(): 0, (48.4% identity in 709 aa overlap); similar to GLGX_HAEIN|P45178 glycogen operon protein glgx (659 aa), FASTA scores: opt: 1512, E(): 0, (42.3% identity in 645 aa overlap); Belongs to the glycosyl hydrolase 13 family. (721 aa) |
| | Rv1565c | Rv1565c, (MTCY336.38), len: 729 aa. Conserved hypothetical membrane protein, some similarity to O05402 hypothetical 72.2 kDa protein from Bacillus subtilis (634 aa), FASTA results: opt: 384, E(): 4.8e-17, (29.1% identity in 378 aa overlap); and to Y392_HAEIN|P43993 hypothetical protein hi0392 from H. influenzae (245 aa), FASTA results: opt: 265, E(): 5.5e-10, (28.3% identity in 247 aa overlap). C-terminal half equivalent to AL049478|MLCL458_19 (274 aa) (78.5% identity in 274 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0111,Rv0228, Rv1254, Rv0517. N-t [...] (729 aa) |
| | impA | Probable inositol-monophosphatase ImpA (imp); Catalyzes the dephosphorylation of inositol 1-phosphate (I-1- P) to yield free myo-inositol, a key metabolite in mycobacteria. (270 aa) |
| | pykA | Rv1617, (MTCY01B2.09), len: 472 aa. Probable pykA,pyruvate kinase. FASTA best: Q46078 pyruvate kinase from corynebacterium glutamicum (475 aa), opt: 2221, E(): 0,(72.2% identity in 468 aa overlap). Belongs to the pyruvate kinase family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). (472 aa) |
| | Rv1692 | Probable phosphatase; Glycerol-phosphate phosphatase with a preference for D- glycerol 3-phosphate (sn-glycerol 1-phosphate) over L-glycerol 3- phosphate (sn-glycerol 3-phosphate). Is the final enzyme involved in the recycling/catabolism of glycerophospholipid polar heads. To a lesser extent, is also able to act on glycerol 2-phosphate and D- ribulose 5-phosphate, but cannot use D-glyceraldehyde 3-phosphate, dihydroxyacetone-phosphate, UMP or GMP as substrates. Belongs to the HAD-like hydrolase superfamily. (353 aa) |
| | Rv1760 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (502 aa) |
| | Rv1771 | L-gulono-1,4-lactone dehydrogenase; Oxidizes L-gulono-1,4-lactone to L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate. Can use both cytochrome c and phenazine methosulfate as exogenous electron acceptors, but molecular oxygen does not serve as a substrate. Is very specific for the L- gulono-1,4-lactone substrate, since it can not oxidize L-galactono-1,4- lactone, D-glucurono-3,6-lactone, D-glucuronate, D-arabinose, or D- xylose; Belongs to the oxygen-dependent FAD-linked oxidoreductase family. (428 aa) |
| | malQ | Probable 4-alpha-glucanotransferase MalQ (amylomaltase) (disproportionating enzyme) (D-enzyme); Rv1781c, (MTV049.03c), len: 724 aa. Probable malQ,4-alpha-glucanotransferase, similar to many, e.g. P15977|MALQ_ECOLI 4-alpha-glucanotransferase (694 aa),FASTA scores: opt: 964, E(): 0, (31.8% identity in 694 aa overlap). Belongs to the disproportionating enzyme family. (724 aa) |
| | glcB | Malate synthase G GlcB; Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl- CoA) and glyoxylate to form malate and CoA. (741 aa) |
| | gnd1 | Probable 6-phosphogluconate dehydrogenase Gnd1; Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH. (485 aa) |
| | aceAa | Probable isocitrate lyase AceAa [first part] (isocitrase) (isocitratase) (Icl); Together with AceAb, they could catalyze the formation of succinate and glyoxylate from isocitrate. (367 aa) |
| | Rv1987 | Possible chitinase; Rv1987, (MTCY39.32c), len: 142 aa. Possible chitinase, similar to several e.g. P36909|CHIT_STRLI chitinase c precursor (619 aa) FASTA scores, opt: 324, E(): 1.2e-14, (39.5% identity in 129 aa overlap). (142 aa) |
| | otsB1 | Rv2006, (MTCY39.11c), len: 1327 aa. OtsB1,trehalose-6-phosphate phosphatase (see citations below). Belongs to Glycosyl hydrolases family 65. Note that previously known as otsB. Predicted possible vaccine candidate (See Zvi et al., 2008). (1327 aa) |
| | pfkB | 6-phosphofructokinase PfkB (phosphohexokinase) (phosphofructokinase); Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. (339 aa) |
| | cysQ | Monophosphatase CysQ; Phosphatase with a broad specificity. Its primary physiological function is to dephosphorylate 3'-phosphoadenosine 5'- phosphate (PAP) and 3'-phosphoadenosine 5'-phosphosulfate (PAPS). Thus, plays a role in mycobacterial sulfur metabolism, since it can serve as a key regulator of the sulfate assimilation pathway by controlling the pools of PAP and PAPS in the cell. To a lesser extent, is also able to hydrolyze inositol 1-phosphate (I-1-P), fructose 1,6-bisphosphate (FBP) (to fructose 6-phosphate (F-6-P)) and AMP in vitro, but this might not be significant in vivo. [...] (267 aa) |
| | murG | Undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase; Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II). (410 aa) |
| | dlaT | DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase; Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). Appears to be essential for Mtb pathogenesis. (553 aa) |
| | aceE | Pyruvate dehydrogenase E1 component; Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). AceE has reductase activity with pyruvate but does not react with 2- oxoglutarate. (901 aa) |
| | acpM | Meromycolate extension acyl carrier protein AcpM; Acyl carrier protein involved in meromycolate extension. Belongs to the acyl carrier protein (ACP) family. (115 aa) |
| | glpD1 | Rv2249c, (MTCY427.31c), len: 516 aa. Probable glpD1,glycerol-3-phosphate dehydrogenase, similar to SW:GLPD_ECOLI P13035 aerobic glycerol-3-phosphate dehydrogenase (30.0% identity in 486 aa overlap) and SW:GLPA_ECOLI P13032 anaerobic glycerol-3-phosphate dehydrogenase (28.2% identity in 504 aa overlap). Also similar to Rv3302c|glpD2 glycerol-3-phosphate dehydrogenase. Cofactor: FAD (by similarity). Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. (516 aa) |
| | Rv2285 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (445 aa) |
| | Rv2402 | Conserved protein; Catalyzes the hydrolysis of alpha,alpha-trehalose into two molecules of D-glucose. (642 aa) |
| | rbsK | Ribokinase RbsK; Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway. (304 aa) |
| | rpiB | Ribose-5-phosphate isomerase; Catalyzes the interconversion of ribulose-5-P and ribose-5-P. It has not isomerase activity towards D-allose 6-phosphate. (162 aa) |
| | aglA | Alpha-glucosidase AglA; Rv2471, (MTV008.27), len: 546 aa. Probable aglA,maltase (alpha-glucosidase), highly similar or similar to several e.g. Q60027|AGLA from Thermomonospora curvata (544 aa), FASTA scores: opt: 2071, E(): 4e-116, (57.7% identity in 525 aa overlap); Q9KZE3|AGLAE from Streptomyces coelicolor (534 aa), FASTA scores: opt: 1475, E(): 1.5e-80,(50.1% identity in 537 aa overlap); O86874|AGLA from Streptomyces lividans (534 aa), FASTA scores: opt: 1473,E(): 2e-80, (50.1% identity in 537 aa overlap); etc. Seems to belong to family 13 of glycosyl hydrolases, also known as the a [...] (546 aa) |
| | Rv2484c | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity; Belongs to the long-chain O-acyltransferase family. (491 aa) |
| | aftC | Possible arabinofuranosyltransferase AftC; Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the addition of an arabinofuranosyl (Araf) residue from the sugar donor decaprenyl-phospho-arabinose (DPA) on the C-3 of an alpha-(1->5)-linked Araf from the arabinan backbone of AG. It can also use (Z,Z)- farnesylphosphoryl D-arabinose (Z-FPA), and to a lesser extent (E,E,Z,Z,Z,Z)-heptaprenylphosphoryl D-arabinose (Z-HPA) and (Z)- nerylphosphoryl D-arabinos [...] (433 aa) |
| | suhB | Inositol-1-monophosphatase SuhB; Catalyzes the dephosphorylation of inositol 1-phosphate (I-1- P) to yield free myo-inositol, a key metabolite in mycobacteria. Is also able to hydrolyze a variety of polyol phosphates such as glucitol- 6-phosphate, inositol 2-phosphate (I-2-P), glycerol-2-phosphate, and 2'-AMP, albeit with reduced efficiency. (290 aa) |
| | ppgK | Polyphosphate glucokinase PpgK (polyphosphate-glucose phosphotransferase); Catalyzes the phosphorylation of glucose using polyphosphate or ATP as the phosphoryl donor. Polyphosphate, rather than ATP, seems to be the major phosphate donor for the enzyme in M.tuberculosis (By similarity); Belongs to the ROK (NagC/XylR) family. (265 aa) |
| | pca | Probable pyruvate carboxylase Pca (pyruvic carboxylase); Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. (1127 aa) |
| | Rv2974c | Rv2974c, (MTCY349.13), len: 470 aa. Conserved hypothetical ala-rich protein, highly similar to others e.g. C-terminus of Q9ZBR4|SC7A1.09 hypothetical 59.5 KDA protein from Streptomyces coelicolor (589 aa), FASTA scores: opt: 774, E(): 1.3e-36, (41.0% identity in 495 aa overlap); Q9K9Z6|BH2498 hypothetical protein from Bacillus halodurans (557 aa), FASTA scores: opt: 268, E(): 8e-08,(27.7% identity in 502 aa overlap) (N-terminus longer 76 aa); Q9X293 conserved hypothetical protein from Thermotoga maritima (497 aa), FASTA scores: opt: 265, E(): 1.1e-07,(24.9% identity in 470 aa overlap) [...] (470 aa) |
| | Rv2975c | Rv2975c, (MTCY349.12), len: 84 aa. Conserved hypothetical protein, similar to N-terminus of others e.g. Q9ZBR4|SC7A1.09 hypothetical 59.5 KDA protein from Streptomyces coelicolor (589 aa), FASTA scores: opt: 141,E(): 0.0019, (41.25% identity in 80 aa overlap); Q98R49|MYPU_1610 hypothetical protein from Mycoplasma pulmonis (545 aa), FASTA scores: opt: 127, E(): 0.023,(48.0% identity in 50 aa overlap); Q9K9Z6|BH2498 hypothetical protein from Bacillus halodurans (557 aa),FASTA scores: opt: 126, E(): 0.028, (34.55% identity in 81 aa overlap); etc. Also some similarity with N-terminus of P4 [...] (84 aa) |
| | gpdA2 | Glycerol-3-phosphate dehydrogenase [NAD(P)+]; Rv2982c, (MTCY349.05), len: 334 aa. Probable gpdA2 (alternate gene name: gpsA), glycerol-3-phosphate dehydrogenase [NAD(P)+], equivalent to Q9CBR9|GPDA_MYCLE glycerol-3-phosphate dehydrogenase [NAD(P)+] from Mycobacterium leprae (349 aa), FASTA scores: opt: 1686,E(): 1.7e-95, (77.95% identity in 349 aa overlap). Also highly similar to others e.g. Q9ZBS0|GPDA_STRCO from Streptomyces coelicolor (336 aa), FASTA scores: opt: 1165,E(): 9.8e-64, (56.25% identity in 327 aa overlap); P46919|GPDA_BACSU from Bacillus subtilis (345 aa), FASTA scores: [...] (334 aa) |
| | fbiD | Conserved hypothetical alanine rich protein; Guanylyltransferase that catalyzes the activation of phosphoenolpyruvate (PEP) as enolpyruvoyl-2-diphospho-5'-guanosine, via the condensation of PEP with GTP. It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor. (214 aa) |
| | pfkA | Probable 6-phosphofructokinase PfkA (phosphohexokinase) (phosphofructokinase); Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis; Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily. (343 aa) |
| | Rv3031 | Conserved protein; Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position (Probable). Is probably involved in the biosynthesis of 6-O-methylglucosyl lipopolysaccharides (MGLP); Belongs to the glycosyl hydrolase 57 family. (526 aa) |
| | Rv3032 | Alpha (1->4) glucosyltransferase; Glucosyltransferase that uses UDP-glucose as the sugar donor to elongate alpha-(1->4)-glucans. Is involved in the biosynthesis of both 6-O-methylglucosyl lipopolysaccharides (MGLP) and glycogen. May also use ADP-glucose as substrate. (414 aa) |
| | pgmA | Rv3068c, (MTCY22D7.13), len: 547 aa. Probable pgmA,phosphoglucomutase, highly similar to other phosphoglucomutases e.g. Q9L117|PGM from Streptomyces coelicolor (546 aa), FASTA scores: opt: 2569, E(): 2.8e-149, (71.4% identity in 545 aa overlap); Q9ABY5|CC0085 from Caulobacter crescentus (545 aa), FASTA scores: opt: 2465, E(): 6.2e-143, (70.4% identity in 541 aa overlap); P38569|PGMU_ACEXY|CELB from Acetobacter xylinum (555 aa),FASTA scores: opt: 2206, E(): 4e-127, (62.25% identity in 543 aa overlap); P74643|PGM|SLL0726 from Synechocystis sp. strain PCC 6803 (567 aa), FASTA scores: opt: [...] (547 aa) |
| | Rv3087 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity. (472 aa) |
| | tgs4 | Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs4; Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmitoyl-CoA. (474 aa) |
| | tgs1 | Triacylglycerol synthase (diacylglycerol acyltransferase) Tgs1; Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (Probable). (463 aa) |
| | hisN | Probable monophosphatase; Catalyzes the dephosphorylation of histidinol-phosphate to histidinol, the direct precursor of histidine; Belongs to the inositol monophosphatase superfamily. (260 aa) |
| | gpm2 | Possible phosphoglycerate mutase Gpm2 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM); Phosphatase with a broad specificity. Can dephosphorylate a variety of substrates including phosphorylated sugars like fructose-6- phosphate (F6P). Is able to function in vivo as a fructose-1,6-bisphosphatase (FBPase) and to maintain gluconeogenesis when the classical FBPase GlpX is absent. Shows negligible phosphoglycerate mutase activity. Has no phosphatase activity against 3-phosphoglycerate, 2,3- bisphosphoglycerate, or hydrophobic substrates such as alpha-napthyl phosphate. (203 aa) |
| | tgs3 | Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs3; Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). Belongs to the long-chain O-acyltransferase family. (271 aa) |
| | manA | Mannose-6-phosphate isomerase ManA; Rv3255c, (MTCY20B11.30c), len: 408 aa. Probable manA, mannose-6-phosphate isomerase, equivalent to Q9CCJ5|MANA|ML0765 putative mannose-6-phosphate isomerase from Mycobacterium leprae (410 aa), FASTA scores: opt: 2271, E(): 1.6e-133, (84.45% identity in 411 aa overlap). Also similar to many others e.g. Q9KZL9|MANA from Streptomyces coelicolor (383 aa), FASTA scores: opt: 946,E(): 2.4e-51, (44.4% identity in 403 aa overlap); Q9KV87|VC0269 from Vibrio cholerae (399 aa), FASTA scores: opt: 726, E(): 1.1e-37, (34.15% identity in 404 aa overlap); Q9CMJ5|PM [...] (408 aa) |
| | pmmA | Rv3257c, (MTV015.02c), len: 465 aa. Probable pmmA,phosphomannomutase, equivalent to Q9CCJ7|PMMA|ML0763 phosphomannomutase from Mycobacterium leprae (468 aa),FASTA scores: opt: 2533, E(): 2e-145, (83.1% identity in 468 aa overlap). Also similar to many e.g. Q9KZL6|MANB from Streptomyces coelicolor (454 aa), FASTA scores: opt: 1820,E(): 2e-102, (63.2% identity in 459 aa overlap); Q9PGN8|XF0260 from Xylella fastidiosa (500 aa), FASTA scores: opt: 1085, E(): 4.7e-58, (40.7% identity in 462 aa overlap); Q9EY19|MANB from Salmonella enterica subsp. arizonae (456 aa), FASTA scores: opt: 988, E [...] (465 aa) |
| | fbiA | Probable F420 biosynthesis protein FbiA; Catalyzes the transfer of the phosphoenolpyruvate moiety from enoylpyruvoyl-2-diphospho-5'-guanosine (EPPG) to 7,8-didemethyl-8- hydroxy-5-deazariboflavin (FO) with the formation of dehydro coenzyme F420-0 and GMP; Belongs to the CofD family. (331 aa) |
| | fbiB | Probable F420 biosynthesis protein FbiB; Bifunctional enzyme that catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L- lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives, and the FMNH2- dependent reduction of dehydro-F420-0 to form F420-0. (448 aa) |
| | wbbL1 | N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase; Involved in the biosynthesis of the mycolylarabinogalactan- peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the transfer of the rhamnosyl moiety from dTDP-rhamnosyl (dTDP-Rha) onto the decaprenyl-pyrophosphoryl- GlcNAc (C50-PP-GlcNAc), yielding rhamnosyl-decaprenyl-pyrophosphoryl- GlcNAc (Rha-C50-PP-GlcNAc); Belongs to the glycosyltransferase 2 family. (301 aa) |
| | rmlD | dTDP-4-dehydrorhamnose reductase; Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage. Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L- rhamnose (By similarity). (304 aa) |
| | Rv3277 | Rv3277, (MTCY71.17), len: 272 aa. Probable conserved transmembrane protein, equivalent, but longer 49 aa, to Q49673|B1308_C1_121|ML0734 putative membrane protein from Mycobacterium leprae (228 aa), FASTA scores: opt: 1266,E(): 6.1e-78, (84.2% identity in 228 aa overlap). Also similar to various proteins (principally unknowns) e.g. Q9KZ84|SCE25.02 putative integral membrane protein from Streptomyces coelicolor (190 aa), FASTA scores: opt: 197,E(): 3.6e-06, (32.0% identity in 150 aa overlap); BAB50058|MLL3086 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (136 aa), FASTA s [...] (272 aa) |
