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| | leuS | Probable leucyl-tRNA synthetase LeuS (leucine--tRNA ligase) (LEURS); Rv0041, (MTCY21D4.04), len: 969 aa. Probable leucyl-tRNA synthetase, similar to many. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. (969 aa) |
| | rpsF | 30S ribosomal protein S6 RpsF; Binds together with S18 to 16S ribosomal RNA. (96 aa) |
| | rpsR1 | 30S ribosomal protein S18-1 RpsR1; Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit; Belongs to the bacterial ribosomal protein bS18 family. (84 aa) |
| | rplI | 50S ribosomal protein L9 RplI; Binds to the 23S rRNA. (152 aa) |
| | dnaB | Probable replicative DNA helicase DnaB; Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity; Belongs to the helicase family. DnaB subfamily. (874 aa) |
| | rpmB1 | Rv0105c, (MTCY251.24c), len: 94 aa. rpmB1, 50S ribosomal protein L28-1, highly similar to others e.g. Q9X8K8|R28B_STRCO 50S ribosomal protein L28-2 from Streptomyces coelicolor (78 aa); RL28_ECOLI|P02428 50s ribosomal protein l28 from Escherichia coli (77 aa), FASTA scores: opt: 167, E(): 6.2e-06, (40.7% identity in 59 aa overlap); etc. Also similar to MTCY63A_2 from Mycobacterium tuberculosis. Belongs to the L28P family of ribosomal proteins. (94 aa) |
| | sigG | Probable alternative RNA polymerase sigma factor SigG (RNA polymerase ECF type sigma factor); Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by a cognate anti-sigma factor until released, although no anti-sigma factor is known for this protein. May be involved in host intracellular survival after infection (strains H37Rv and CDC 1551). A role in the SOS response is controversial; it has been seen in strain CDC 1551 but not i [...] (370 aa) |
| | zmp1 | Rv0198c, (MTV033.06c), len: 663 aa. Probable zmp1,zinc metalloprotease, equivalent to Z95398|MLCL622.12c from Mycobacterium leprae (667 aa), FASTA scores: opt: 3710,E(): 0, (80.8 % identity in 667 aa overlap). Also similar to many other metalloproteases e.g. members of the eukaryotic neprilysin family: P08473|NEP_HUMAN neprilysin (749 aa), FASTA scores: opt: 872, E(): 0, (31.1% identity in 692 aa overlap); Q07744|PEPO_LACLA neutral endopeptidase from Lactococcus lactis (626 aa), FASTA scores: opt: 862,E(): 0, (30.0% identity in 654 aa overlap). Contains PS00142 Neutral zinc metallopept [...] (663 aa) |
| | trmB | Hypothetical methlytransferase (methylase); Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. (263 aa) |
| | Rv0235c | Rv0235c, (MTCY08D5.31c), len: 482 aa. Probable conserved transmembrane protein, highly similar to AL133278|CAB61913.1|SCM11_2 putative integral membrane protein from Streptomyces coelicolor (470 aa), FASTA scores: opt: 2116, E(): 0, (61.8% identity in 474 aa overlap); and similar to hypothetical proteins from other organisms e.g. Q13392|384D8_7 hypothetical protein (579 aa), FASTA scores: opt: 355, E(): 6.9e-17, (28.5% identity in 569 aa overlap). (482 aa) |
| | mycP3 | Mycosin-3; Rv0291, (MTV035.19), len: 461 aa. Probable mycP3,membrane-anchored serine protease (mycosin) (see Brown et al., 2000), similar to several others in mycobacteria e.g. Z94121|MTY15F10_28|Rv1796 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 1168, E(): 0, (44.6% identity in 453 aa overlap); Rv3886c; Rv3883c; Rv3449; and Y14967|MLCB628_4|MLCB628.04 from Mycobacterium leprae (446 aa), FASTA scores: opt: 1159, E(): 0, (43.5 identity in 446 aa overlap). Has signal sequence and hydrophobic stretch at C-terminus, followed by short positively charged segment,that seems t [...] (461 aa) |
| | Rv0380c | Rv0380c, (MTV036.15c), len: 183 aa. Possible RNA methyltransferase, equivalent to CAC32002.1|AL583925 possible RNA methyltransferase from Mycobacterium leprae (182 aa). Also some similarity with others methyltransferases e.g. P19396|TRMH_ECOLI|78514|JV0043 tRNA (guanosine-2'-O-)-methyltransferase (tRNA methyltransferase) from Escherichia coli (229 aa), FASTA scores: opt: 227, E(): 1.4e-09, (28.9% identity in 166 aa overlap). Also similar to Rv0881, Rv3579c, Rv1644 from Mycobacterium tuberculosis. (183 aa) |
| | def | Probable polypeptide deformylase Def (PDF) (formylmethionine deformylase); Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity); Belongs to the polypeptide deformylase family. (197 aa) |
| | sigK | Alternative RNA polymerase sigma factor SigK; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis. Sigma-K controls genes such as mpt70 and mpt83. (187 aa) |
| | rpmG2 | Rv0634B, len: 55 aa. rpmG2, 50S ribosomal protein L33. Note that Mycobacterium tuberculosis has a second rpmG gene: P96925|R33H_MYCTU|Rv2057c|MTCY63A.03|rpmG1 putative 50S ribosomal protein L33 (55 aa), FASTA scores: opt: 391,E(): 2.9e-25, (100.0% identity in 55 aa overlap). Belongs to the L33P family of ribosomal proteins. (55 aa) |
| | nusG | Probable transcription antitermination protein NusG; Participates in transcription elongation, termination and antitermination; Belongs to the NusG family. (238 aa) |
| | rplK | 50S ribosomal protein L11 RplK; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. (142 aa) |
| | rplA | 50S ribosomal protein L1 RplA; Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release. (235 aa) |
| | rplJ | 50S ribosomal protein L10 RplJ; Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. (178 aa) |
| | rplL | 50S ribosomal protein L7/L12 RplL (SA1); Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation; Belongs to the bacterial ribosomal protein bL12 family. (130 aa) |
| | Rv0658c | Rv0658c, (MTCI376.18), len: 238 aa. Probable conserved integral membrane protein, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and showing some similarity with P33774|YPRB_ECOLI hypothetical 24.3 kDa protein from Escherichia coli (217 aa), FASTA scores: opt: 174, E(): 5.3e-05, (25.6% identity in 223 aa overlap). Also similar to Rv1863c and Rv0804 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (238 aa) |
| | rpoB | DNA-directed RNA polymerase subunit beta; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1172 aa) |
| | rpoC | DNA-directed RNA polymerase subunit beta; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1316 aa) |
| | Rv0674 | Rv0674, (MTV040.02), len: 240 aa. Conserved hypothetical protein, highly similar to AC13063.1|AL445503 conserved hypothetical protein from Streptomyces coelicolor (268 aa); and similar to NP_438100.1|NC_003078 putative regulator of phenylacetic acid degradation ArsR family protein from Sinorhizobium meliloti (306 aa) and other proteins e.g. AB011837|AB011837_13 hypothetical protein from Bacillus halodurans (298 aa), FASTA scores: opt: 148,E(): 0.0081, (25.1% identity in 235 aa overlap); etc. (240 aa) |
| | rpsL | 30S ribosomal protein S12 RpsL; With S4 and S5 plays an important role in translational accuracy. (124 aa) |
| | rpsG | 30S ribosomal protein S7 RpsG; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA; Belongs to the universal ribosomal protein uS7 family. (156 aa) |
| | fusA1 | Probable elongation factor G FusA1 (EF-G); Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity); Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor [...] (701 aa) |
| | tuf | Probable iron-regulated elongation factor TU Tuf (EF-TU); This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (396 aa) |
| | rpsJ | 30S ribosomal protein S10 RpsJ (transcription antitermination factor NusE); Involved in the binding of tRNA to the ribosomes. Belongs to the universal ribosomal protein uS10 family. (101 aa) |
| | rplC | 50S ribosomal protein L3 RplC; One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit; Belongs to the universal ribosomal protein uL3 family. (217 aa) |
| | rplD | 50S ribosomal protein L4 RplD; One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome. (223 aa) |
| | rplW | 50S ribosomal protein L23 RplW; One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome. (100 aa) |
| | rplB | 50S ribosomal protein L2 RplB; One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome. Belongs to the universal ribosomal protein uL2 family. (280 aa) |
| | rpsS | 30S ribosomal protein S19 RpsS; Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. (93 aa) |
| | rplV | 50S ribosomal protein L22 RplV; This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity). (197 aa) |
| | rpsC | 30S ribosomal protein S3 RpsC; Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation; Belongs to the universal ribosomal protein uS3 family. (274 aa) |
| | rplP | 50S ribosomal protein L16 RplP; Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs; Belongs to the universal ribosomal protein uL16 family. (138 aa) |
| | rpmC | Rv0709, (MTCY210.28), len: 77 aa. rpmC, 50S ribosomal protein L29, equivalent to O06050|RL29_MYCBO|MBS10OPER_10 50S ribosomal protein L29 from Mycobacterium bovis BCG (75 aa); and O32989|RL29_MYCLE|MLCB2492_10 50S ribosomal protein L29 from Mycobacterium leprae (80 aa). Also highly similar to others e.g. Q9L0D2|RL29_STRCO 50S ribosomal protein L29 from Streptomyces coelicolor (74 aa); P12873|RL29_BACSU 50s ribosomal protein l29 from Bacillus subtilis (66 aa), FASTA scores: opt: 225, E(): 8.3e-11, (58.6% identity in 58 aa overlap); etc. Belongs to the L29P family of ribosomal proteins. (77 aa) |
| | rpsQ | 30S ribosomal protein S17 RpsQ; One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. (136 aa) |
| | rplN | 50S ribosomal protein L14 RplN; Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome; Belongs to the universal ribosomal protein uL14 family. (122 aa) |
| | rplX | 50S ribosomal protein L24 RplX; One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. (105 aa) |
| | rplE | 50S ribosomal protein L5 RplE; This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. (187 aa) |
| | rpsN1 | 30S ribosomal protein S14 RpsN1; Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site. (61 aa) |
| | rpsH | 30S ribosomal protein S8 RpsH; One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; Belongs to the universal ribosomal protein uS8 family. (132 aa) |
| | rplF | 50S ribosomal protein L6 RplF; This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center; Belongs to the universal ribosomal protein uL6 family. (179 aa) |
| | rplR | 50S ribosomal protein L18 RplR; This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. (122 aa) |
| | rpsE | 30S ribosomal protein S5 RpsE; With S4 and S12 plays an important role in translational accuracy; Belongs to the universal ribosomal protein uS5 family. (220 aa) |
| | rpmD | Rv0722, (MTCY210.41), len: 65 aa. rpmD, 50S ribosomal protein L30, equivalent to O33001 ribosomal protein L30 from Mycobacterium leprae (71 aa). Also highly similar to others e.g. P46789|RL30_STRCO 50S ribosomal protein L30 from Streptomyces coelicolor (60 aa); P02430|RL30_ECOLI 50S ribosomal protein L30 from Escherichia coli (58 aa), FASTA scores: opt: 168, E(): 1.5e-13, (53.7% identity in 54 aa overlap); etc. Belongs to the L30P family of ribosomal proteins. (65 aa) |
| | rplO | 50S ribosomal protein L15 RplO; Binds to the 23S rRNA; Belongs to the universal ribosomal protein uL15 family. (146 aa) |
| | sppA | Rv0724, (MTCY210.43), len: 623 aa. Possible sppA,protease IV (endopeptidase IV), equivalent (but longer 23 aa) to MLCB2492_24|O33003 endopeptidase IV from Mycobacterium leprae (602 aa). Also similar to others e.g. NP_419743.1|NC_002696 signal peptide peptidase SppA from Caulobacter crescentus (594 aa); P08395|SPPA_ECOLI|B1766 protease IV (endopeptidase) from Escherichia coli strain K-12 (618 aa), FASTA scores: opt: 582, E(): 8.9e-27, (34.1% identity in 525 aa overlap); etc. Belongs to peptidase family S49. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; [...] (623 aa) |
| | mapA | Methionine aminopeptidase MapA (map) (peptidase M) (MetAP); Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed. (266 aa) |
| | sigL | Probable alternative RNA polymerase sigma factor SigL; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis. Over-expression of SigL induces 19-28 genes including polyketide synthases, secreted and membrane proteins. Might play a minor role in regulating SigB. (177 aa) |
| | Rv0802c | Possible succinyltransferase in the GCN5-related N-acetyltransferase family; May function as a succinyl-CoA transferase. (218 aa) |
| | Rv0804 | Rv0804, (MTCY07H7A.05c), len: 209 aa. Conserved hypothetical protein, showing similarity with C-terminus of Rv1863c|MTCY359.10 conserved hypothetical protein from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 199, E(): 1.2e-05, (33.2% identity in 220 aa overlap); and Rv0658c. Contains PS01151 Fimbrial biogenesis outer membrane usher protein signature. (209 aa) |
| | mshD | GCN5-related N-acetyltransferase, MshD; Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol. Belongs to the acetyltransferase family. MshD subfamily. (315 aa) |
| | dus | Possible transcriptional regulatory protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines; Belongs to the Dus family. (389 aa) |
| | Rv0828c | Possible deaminase; Rv0828c, (MTV043.20c), len: 140 aa. Possible deaminase, with its N-terminus highly similar to middle part of NP_302602.1|NC_002677 possible cytidine/deoxycytidylate deaminase from Mycobacterium leprae (171 aa). Also similar to other deaminases e.g. CAC18715.2|AL451182 putative deaminase from Streptomyces coelicolor (167 aa); NP_251189.1|NC_002516 probable deaminase from Pseudomonas aeruginosa (151 aa); NP_108387.1|NC_002678 nitrogen fixation protein gene from Mesorhizobium loti (149 aa); etc. Also similar to many conserved hypothetical proteins e.g. NP_389200.1|NC_0 [...] (140 aa) |
| | Rv0881 | Rv0881, (MTCY31.09), len: 288 aa. Possible rRNA methyltransferase, highly similar to others and hypothetical proteins e.g. CAB76071.1|AL157953 putative rRNA methylase from Streptomyces coelicolor (272 aa); NP_421117.1|NC_002696 spoU rRNA methylase family protein from Caulobacter crescentus (268 aa); D90913_93|P74261 rRNA methylase from Synechocystis sp. (274 aa), FASTA scores: E(): 1.1e-13, (26.3% identity in 278 aa overlap); P18644|TSNR_STRCN rRNA methyltransferase from Streptomyces cyaneus (Streptomyces curacoi) (269 aa), FASTA scores: E(): 3.7e-08, (23.9% identity in 268 aa overlap) [...] (288 aa) |
| | rpmF | Rv0979A, len: 57 aa. rpmF, 50S ribosomal protein L32, similar to others e.g. rpmF|Q9RL50 probable 50S ribosomal protein from Streptomyces coelicolor (56 aa),FASTA scores: E(): 5.1e-09, (63.45% identity in 52 aa overlap); etc. Belongs to the L32P family of ribosomal proteins. (57 aa) |
| | rsmI | Conserved protein; Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA; Belongs to the methyltransferase superfamily. RsmI family. (285 aa) |
| | metS | Methionyl-tRNA synthetase MetS (MetRS) (methionine--tRNA ligase); Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation; Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. (519 aa) |
| | ksgA | Ribosomal RNA small subunit methyltransferase A; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. (317 aa) |
| | pth | Probable peptidyl-tRNA hydrolase Pth; The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Belongs to the PTH family. (191 aa) |
| | rplY | 50S ribosomal protein L25 RplY; This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance. Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily. (215 aa) |
| | greA | Probable transcription elongation factor GreA (transcript cleavage factor GreA); Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. (164 aa) |
| | Rv1140 | Rv1140, (MTCI65.07), len: 282 aa. Probable integral membrane protein. Weak similarity in C-terminus to hypothetical Escherichia coli proteins YPRA and YPRB,possibly membrane-bound e.g. YPRA_ECOLI hypothetical 24.3 kDa protein (URF 1) (217 aa), FASTA scores: opt: 166, E(): 0.00062, (31.0% identity in 158 aa overlap). (282 aa) |
| | Rv1147 | Conserved protein; Rv1147, (MTCI65.14), len: 216 aa. Conserved protein,similar to many conserved hypothetical proteins, and some similarity to several methyltransferases e.g. Q05197|PMTA_RHOSH phosphatidylethanolamine N-methyltransferase from R. sphaeroides (203 aa), FASTA scores: opt: 156, E(): 0.00073, (27.6% identity in 156 aa overlap). (216 aa) |
| | typA | GTP-binding protein TypA/BipA; Rv1165, (MTV005.01-MTCI65.32), len: 628 aa. Possible typA (alternate gene name: bipA), GTP-binding translation elongation factor, similar to several e.g. P32132|TYPA_ECOLI|BIPA|B387 Escherichia coli (591 aa); YIHK_SYNY3|P72749 gtp-binding protein TYPA/BIPA homolog from synechocystis sp. (597 aa), FASTA scores: E(): 0,(46.9% identity in 610 aa overlap); and to elongation factor EF-G from many organims e.g. EFG_MICLU|P09952 micrococcus luteus (701 aa), FASTA scores: E(): 3e-24,(29.8% identity in 500 aa overlap). Belongs to the GTP-binding elongation factor [...] (628 aa) |
| | sigI | Possible alternative RNA polymerase sigma factor SigI; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by a cognate anti-sigma factor until released, although no anti-sigma factor is known for this protein (By similarity). (290 aa) |
| | sigE | Alternative RNA polymerase sigma factor SigE; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released. Responds to heat shock and surface stress (detergent exposure). When combined with isolated core RNA polymerase from M.smegmatis is able to guide initiation from the sigB promoter. Required for full expression of sigB, and for sigB induction after detergent exposure but not after heat shock. Con [...] (257 aa) |
| | argS | Rv1292, (MTCY373.12), len: 550 aa. Probable argS,Arginyl-tRNA synthetase, highly similar to SYR_MYCLE|P45840 Mycobacterium leprae (550 aa), FASTA scores: opt: 3115,E(): 0, (84.9% identity in 550 aa overlap). Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. (550 aa) |
| | rho | Probable transcription termination factor Rho homolog; Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA- dependent ATPase activity, and release of the mRNA from the DNA template. Shows poor RNA-dependent ATP hydrolysis and inefficient DNA- RNA unwinding activities, but exhibits robust and fast transcription termination, which suggests that the transcription termination function of M.tuberculosis Rho is not correlated with its helicase/translocase activities and that these functions may not be important for its RN [...] (602 aa) |
| | rpmE | 50S ribosomal protein L31 RpmE; Binds the 23S rRNA. (80 aa) |
| | prfA | Probable peptide chain release factor 1 PrfA (RF-1); Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA. (357 aa) |
| | Rv1301 | Conserved protein; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. (217 aa) |
| | Rv1339 | Conserved protein; Rv1339, (MTCY130.24), len: 273 aa. Conserved protein, highly similar to Y211_MYCLE|P50474 hypothetical protein b1549_c2_211 from Mycobacterium leprae (284 aa),FASTA scores: opt: 1672, E(): 0, (86.2% identity in 276 aa overlap). Also similar to AL096852|SCE19A.08 hypothetical protein from Streptomyces coelicolor (250 aa), FASTA scores: opt: 630, E(): 0, (42.2% identity in 256 aa overlap). Similar to M. tuberculosis hypothetical proteins Rv3796, Rv2407. Predicted to be an outer membrane protein (See Song et al., 2008). (273 aa) |
| | rphA | Probable ribonuclease RphA (RNase PH) (tRNA nucleotidyltransferase); Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. (259 aa) |
| | moeY | Rv1355c, (MTCY02B10.19c), len: 715 aa. Possible moeY, Molybdopterin biosynthesis protein, very weak similarity to MOEB_ECOLI|P12282 molybdopterin biosynthesis moeb protein (249 aa), FASTA scores, opt: 180, E(): 8.5e-05, (29.3% identity in 174 aa overlap). (715 aa) |
| | rpoZ | DNA-directed RNA polymerase subunit omega; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. (110 aa) |
| | fmt | Probable methionyl-tRNA formyltransferase Fmt; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. (312 aa) |
| | fmu | Probable Fmu protein (sun protein); May act as RNA methyltransferase. (457 aa) |
| | Rv1461 | Conserved protein; Rv1461, (MTV007.08), len: 846 aa. Conserved protein. Equivalent of spliced protein from Mycobacterium leprae MLCL536.28c len: 869. Residues 1-253 represent N-extein,and 613-846 the C-extein. The intein present from residues 254 - 612 is different in sequence and site of the insertion from the one present in MLCL536.28c. FASTA scores: Z99125|MLCL536_23 Mycobacterium leprae cosmid L536 (869 aa), opt: 1498 E(): 0, (54.1% identity in 917 aa overlap). The mature protein is similar to Z99120|BSUB0017_150 hypothetical Bacillus subtilis protein (465 aa), FASTA scores: opt:10 [...] (846 aa) |
| | ileS | Isoleucyl-tRNA synthetase IleS; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity). (1041 aa) |
| | Rv1540 | Rv1540, (MTCY48.25c), len: 308 aa. Member of the yabO/yceC/yfiI family of hypothetical proteins, similar to P44445|YFII_HAEIN hypothetical protein HI0176 from Haemophilus influenzae (324 aa), FASTA scores: opt: 437,E(): 1.2e-22, (33.2% identity in 322 aa overlap). Equivalent to AL049478|MLCL458_13 hypothetical protein from Mycobacterium leprae (308 aa), (89.3% identity in 307 aa overlap). Contains PS01129 hypothetical yabO/yceC/yfiI family signature. (308 aa) |
| | rpsA | 30S ribosomal protein S1 RpsA; Binds mRNA, facilitating recognition of most mRNAs by the 30S ribosomal subunit during translation initiation (By similarity). Probably plays a role in trans-translation; binds tmRNA (the product of the ssrA gene). In trans-translation Ala- aminoacylated transfer-messenger RNA (tmRNA, product of the ssrA gene; the 2 termini fold to resemble tRNA(Ala) while it encodes a short internal open reading frame (the tag peptide)) acts like a tRNA, entering the A-site of the ribosome and displacing the stalled mRNA (which is subsequently degraded). The ribosome the [...] (481 aa) |
| | lysX | Lysyl-tRNA synthetase 2 LysX; Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lys [...] (1172 aa) |
| | infC | Probable initiation factor if-3 InfC; IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. (201 aa) |
| | rpmI | Rv1642, (MTCY06H11.06), len: 64 aa. rpmI, 50S ribosomal protein L35, similar to several e.g. RL35_SYNY3|P48959 from Synechocystis sp. (67 aa), fasta scores: opt: 179, E(): 2.7e-08, (51.6% identity in 64 aa overlap). Belongs to the L35P family of ribosomal proteins. (64 aa) |
| | rplT | 50S ribosomal protein L20 RplT; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity). (129 aa) |
| | tsnR | Rv1644, (MTCY06H11.08), len: 260 aa. Possible tsnR,23S rRNA methyltransferase, similar to several e.g. TSNR_STRLU|P52393 from Streptomyces laurentii (270 aa),FASTA scores: opt: 276, E(): 3.6e-11, (27.6% identity in 261 aa overlap). Also similar to M. tuberculosis hypothetical proteins Rv0881, Rv3579c, and Rv0380c; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (260 aa) |
| | pheS | Rv1649, (MTCY06H11.14), len: 341 aa. Probable pheS,Phenylalanyl-tRNA synthetase alpha chain, similar to several e.g. SYFA_ECOLI|P08312 from Escherichia coli (327 aa), FASTA scores: opt: 978, E(): 0, (46.5% identity in 331 aa overlap). Homology suggests this start site, but there is a potential rbs upstream of a gtg 30 bp upstream; contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. PHE-tRNA synthetase alpha chain subfamily 1. (341 aa) |
| | pheT | Rv1650, (MTCY06H11.15), len: 831 aa. Probable pheT,Phenylalanyl-tRNA synthetase beta chain, similar to several e.g. SYFB_ECOLI|P07395 from Escherichia coli (795 aa),FASTA scores: opt: 995, E(): 0, (31.8% identity in 847 aa overlap). Belongs to the phenylalanyl-tRNA synthetase beta chain family - subfamily 1. (831 aa) |
| | tyrS | Probable tyrosyl-tRNA synthase TyrS (TYRRS); Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). (424 aa) |
| | tlyA | 2'-O-methyltransferase TlyA; Acts as a host evasion factor, that significantly contributes to the pathogenesis of M.tuberculosis by modulating adaptive immune responses by inhibiting host-protective Th1 and Th17 cytokine responses as well as autophagy. Catalyzes the 2'-O-methylation at nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA. Is likely involved in ribosomal biogenesis. Also exhibits hemolytic activity in vitro, by binding with and oligomerizing into host cell membranes. Belongs to the TlyA family. (268 aa) |
| | Rv1711 | Rv1711, (MTCI125.33), len: 254 aa. Conserved hypothetical protein, highly similar to a large family of hypothetical proteins e.g. P37765|YCIL_ECOLI from Escherichia coli (291 aa), FASTA scores: opt: 496, E(): 1.1e-29, (41.6% identity in 250 aa overlap); 9S232|SCI51.08C|AL109848 putative pseudouridine synthase from Streptomyces coelicolor (371 aa), FASTA scores: opt: 818, E(): 0, (53.1% identity in 245 aa overlap). Equivalent to O05668|MLCB1351.03C|Z95117 Hypothetical protein from Mycobacterium leprae (256 aa), (80.5% identity in 256 aa overlap). Contains PS01149 Hypothetical yciL/yejD/ [...] (254 aa) |
| | mycP5 | Mycosin-5; Rv1796, (MTV049.18), len: 585 aa. Probable mycP5,pro-rich membrane-anchored serine protease (mycosin) (see citations below). Member of family with four other Mycobacterium tuberculosis serine proteases: Rv3886c|O05458|MTCY15F10.26|Z94121 (550 aa), FASTA scores: opt: 1173, E(): 0, (47.9% identity in 578 aa overlap); Rv0291, Rv3883c, and Rv3449. Genes all linked to those of ESAT-6 family. Has possible N-terminal signal peptide and hydrophobic anchor-like stretch at C-terminus. Contains two serine protease, subtilase family active site motifs: a aspartic acid active site motif [...] (585 aa) |
| | gcvH | Probable glycine cleavage system H protein GcvH; The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein. (134 aa) |
| | Rv1863c | Rv1863c, (MTCY359.10), len: 256 aa. Probable conserved integral membrane protein, similar to Rv0804|Z95618|MTCY7H7A.05 Hypothetical protein from Mycobacterium tuberculosis (209 aa), FASTA scores: opt: 199, E(): 1e-06, (33.2% identity in 220 aa overlap); and Rv0658c. (256 aa) |
| | erm(37) | Rv1988, (MTCY39.31c), len: 179 aa. Probable erm(37),23S rRNA methyltransferase, similar to ERME_SACER|P07287 rrna adenine n-6-methyltransferase (370 aa), FASTA scores: opt: 259, E(): 2e-11, (35.1% identity in 171 aa overlap); contains PS00092 N-6 Adenine-specific DNA methylases signature. Also similar to Mycobacterium tuberculosis Rv1010 ksgA 16S rRNA dimethyltransferase. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007); Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. (179 aa) |
| | rpsR2 | 30S ribosomal protein S18 RpsR2; Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit; Belongs to the bacterial ribosomal protein bS18 family. (88 aa) |
| | rpsN2 | 30S ribosomal protein S14 RpsN2; Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site; Belongs to the universal ribosomal protein uS14 family. (101 aa) |
| | rpmG1 | Rv2057c, (MTCY63A.03), len: 54 aa. rpmG1, 50S ribosomal protein L33, similar to many. Note that previously known as rpmG; Belongs to the bacterial ribosomal protein bL33 family. (54 aa) |
| | rpmB2 | Rv2058c, (MTCY63A.02), len: 78 aa. rpmB2, 50S ribosomal protein L28, very similar to rL28 of M. tuberculosis. Also similar to rpmB (Rv0105c) of Mycobacterium tuberculosis; Belongs to the bacterial ribosomal protein bL28 family. (78 aa) |
| | Rv2067c | Conserved protein; Rv2067c, (MTCY49.06c), len: 407 aa. Conserved protein, similar to many. (407 aa) |
| | sigC | RNA polymerase sigma factor, ECF subfamily, SigC; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are usually held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis; this sigma factor does not seem to have a cognate anti-sigma factor however. It has been suggested that the sigma domains 2 and 4 may interact via polar residues in this protein to autoregulate. Positively regulates expression of a small regulon [...] (185 aa) |
| | trmI | RNA methyltransferase; Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. (280 aa) |
| | mshC | Cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase MshC; Catalyzes the ATP-dependent condensation of GlcN-Ins and L- cysteine to form L-Cys-GlcN-Ins; Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily. (414 aa) |
| | rsmH | Conserved protein; Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA; Belongs to the methyltransferase superfamily. RsmH family. (396 aa) |
| | Rv2204c | Conserved protein; Rv2204c, (MTCY190.15c), len: 118 aa. Conserved protein. Similar to conserved hypothetical proteins in Actinomycetes and equivalent to Mycobacterium leprae ML0871|ML0871 conserved hypothetical protein (118 aa) and to sp|P45344|YADR_HAEIN hypothetical protein HI1723 (114 aa). FASTA score: ML0871 opt: 720, E(): 8.4e-45; 92.373% identity in 118 aa overlapCAC31252.1| (AL583920); and P45344 opt: 346, E(): 1.8e-18; 45.6% identity in 103 aa overlap. Contains PS01152 Hypothetical hesB/y yadR/yfhF family signature; Belongs to the HesB/IscA family. (118 aa) |
| | lipB | Probable lipoate biosynthesis protein B LipB; Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate- dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate. Belongs to the LipB family. (230 aa) |
| | lipA | Probable lipoate biosynthesis protein A LipA; Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives; Belongs to the radical SAM superfamily. Lipoyl synthase family. (311 aa) |
| | moeW | Rv2338c, (MTCY98.07c), len: 318 aa. Possible moeW,molybdoptenum biosynthesis protein, showing some similarity to several molybdopterin biosynthesis proteins e.g. O27613|MTH1571 molybdopterin biosynthesis protein MOEB homolog from Methanobacterium thermoautotrophicum (251 aa),FASTA scores: opt: 309, E(): 4.7e-14; (30.7% identity in 254 aa overlap); Q9KPQ5|VC2311 HESA/MOEB/THIF family protein from Vibrio cholerae (273 aa), FASTA scores: opt: 255, E(): 4e-09, (36.25% identity in 149 aa overlap); Q9PD34|XF1545 molybdopterin biosynthesis protein from Xylella fastidiosa (276 aa), FASTA score [...] (318 aa) |
| | glyS | Probable glycyl-tRNA synthetase GlyS (glycine--tRNA ligase) (GLYRS); Catalyzes the attachment of glycine to tRNA(Gly). Belongs to the class-II aminoacyl-tRNA synthetase family. (463 aa) |
| | ybeY | Conserved hypothetical protein; Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. (182 aa) |
| | rsmE | Conserved hypothetical protein; Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity); Belongs to the RNA methyltransferase RsmE family. (262 aa) |
| | lepA | Probable GTP-binding protein LepA (GTP-binding elongation factor); Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP- dependent manner. (653 aa) |
| | rnz | Conserved hypothetical protein; Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA; Belongs to the RNase Z family. (273 aa) |
| | rpsT | 30S ribosomal protein S20 RpsT; Binds directly to 16S ribosomal RNA; Belongs to the bacterial ribosomal protein bS20 family. (86 aa) |
| | rpmA | Rv2441c, (MTCY428.05), len: 86 aa. rpmA, 50S ribosomal proteins L27, equivalent to Q9CBZ3|RL27_MYCLE from Mycobacterium leprae (88 aa), FASTA scores: opt: 504,E(): 7.6e-28, (93.2% identity in 81 aa overlap). Also highly similar to others e.g. P95757|RL27_STRGR from Streptomyces griseus (85 aa), FASTA scores: opt: 442, E(): 1.2e-23, (81.5% identity in 81 aa overlap); etc. Contains PS00831 Ribosomal protein L27 signature. Belongs to the L27P family of ribosomal proteins. (86 aa) |
| | rplU | 50S ribosomal protein L21 RplU; This protein binds to 23S rRNA in the presence of protein L20; Belongs to the bacterial ribosomal protein bL21 family. (104 aa) |
| | rne | Rv2444c, (MTCY428.02), len: 953 aa. Possible rne,ribonuclease E, highly similar to others e.g. Q9CBZ1|ML1468 possible ribonuclease from Mycobacterium leprae (924 aa),FASTA scores: opt: 3713, E(): 2.4e-174, (74.2% identity in 966 aa overlap); Q9SI08|AT2G04270 putative ribonuclease E from Arabidopsis thaliana (502 aa), FASTA scores: opt: 674,E(): 7.5e-26, (31.2% identity in 410 aa overlap); etc. Similar at C-terminal end to P21513|RNE_ECOLI|ams|HMP1|B1084 ribonuclease E (RNASE E) from Escherichia coli strain K12 (1061 aa), FASTA scores: opt: 554, E(): 9.9e-20, (37.8% identity in 386 aa o [...] (953 aa) |
| | valS | Valine--tRNA ligase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (876 aa) |
| | ettA | Probable macrolide-transport ATP-binding protein ABC transporter; A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. ATP hydrolysis probably frees it from the ribosome, which can enter the elongation phase. (558 aa) |
| | nusB | N utilization substance protein NusB (NusB protein); Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons. (156 aa) |
| | efp | Probable elongation factor P Efp; Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase (By similarity). (187 aa) |
| | Rv2551c | Rv2551c, (MTCY159.05), len: 139 aa. Conserved hypothetical protein, similar to the second part of Q9XAP1|SC10A7.34c putative type IV peptidase from Streptomyces coelicolor (259 aa), FASTA scores: opt: 243,E(): 7.4e-08, (40.95% identity in 144 aa overlap). Also some similarity with other proteins e.g. AAK58497|GSPO GSPO protein from Acetobacter diazotrophicus (261 aa), FASTA scores: opt: 152, E(): 0.025, (33.35% identity in 135 aa overlap). (139 aa) |
| | Rv2554c | Conserved protein; Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA. (170 aa) |
| | alaS | Probable alanyl-tRNA synthetase AlaS (alanine--tRNA ligase) (alanine translase) (ALARS); Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain; Belongs to the class-II aminoacyl-tRNA synthetase family. (904 aa) |
| | aspS | Probable aspartyl-tRNA synthetase AspS (aspartate--tRNA ligase) (ASPRS) (aspartic acid translase); Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn); Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (596 aa) |
| | hisS | Rv2580c, (MT2657, MTCY227.21), len: 423 aa. Probable hisS, histidyl-tRNA synthetase, equivalent to P46696|SYH_MYCLE|hiss|ML0494|MLCB1259.12|B1177_C3_248 histidyl-tRNA synthetase from Mycobacterium leprae (427 aa), FASTA scores: opt: 2380, E(): 2.1e-131, (85.85% identity in 417 aa overlap). Also highly similar to many e.g. Q9KXP2|hiss from Streptomyces coelicolor (425 aa),FASTA scores: opt: 1542, E(): 1.4e-82, (56.0% identity in 418 aa overlap); O32422|SYH_STAAU|hiss from Staphylococcus aureus (420 aa), FASTA scores: opt: 1135, E(): 7.4e-59,(44.9% identity in 412 aa overlap); P04804|SYH [...] (423 aa) |
| | thrS | Probable threonyl-tRNA synthetase ThrS (threonine-tRNA synthetase)(ThrRS) (threonine-tRNA ligase); Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (692 aa) |
| | Rv2630 | Hypothetical protein; Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently (By similarity); Belongs to the archease family. (179 aa) |
| | rtcB | Conserved hypothetical protein; GTP-dependent RNA ligase that is involved in tRNA splicing and RNA repair. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends. (432 aa) |
| | Rv2689c | Conserved alanine and valine and glycine rich protein; Rv2689c, (MTCY05A6.10c), len: 405 aa (other less probable starts possible). Conserved ala-, val-, gly-rich protein, similar to O54099|SC10A5.06 hypothetical 49.5 KDA protein from Streptomyces coelicolor (458 aa), FASTA scores: opt: 455, E(): 2.7e-20, (38.35% identity in 417 aa overlap); and shows weak similarity in part with several methyltransferases e.g. Q9X0H9|TM1094 putative RNA methyltransferase from Thermotoga maritima (439 aa), FASTA scores: opt: 306, E(): 3e-11, (25.9% identity in 436 aa overlap); AK79403|CAC1435 S-adenosyl [...] (405 aa) |
| | sigA | RNA polymerase sigma factor SigA (sigma-A); Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth (Probable); Belongs to the sigma-70 factor family. RpoD/SigA subfamily. (528 aa) |
| | sigB | RNA polymerase sigma factor SigB; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. A non-essential principal sigma factor that responds to cell envelope stress and hypoxia. Controls a regulon of about 40 genes, with another 100 genes expression being altered during SDS stress and about 50 gene being altered during diamide (oxidative) stress. (323 aa) |
| | miaA | tRNA dimethylallyltransferase; Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A). (314 aa) |
| | miaB | Conserved hypothetical alanine, arginine-rich protein; Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6- (dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine. (512 aa) |
| | recA | RecA protein (recombinase A) [contains: endonuclease PI-MTUI (MTU RecA intein)]; Can catalyze the hydrolysis of ATP in the presence of single- stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage. (790 aa) |
| | rnj | Conserved hypothetical protein; An RNase that has 5'-3' exonuclease and possible endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay (By similarity). Has both beta-lactamase and RNase activity, but the physiological relevance of the beta- lactamase activity, i.e. whether it confers antibiotic resistance, has not been shown ; Belongs to the metallo-beta-lactamase superfamily. RNA- metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily. (558 aa) |
| | gpsI | Polyribonucleotide nucleotidyltransferase; Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction. (752 aa) |
| | rpsO | 30S ribosomal protein S15 RpsO; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA. (89 aa) |
| | truB | tRNA pseudouridine synthase B; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (298 aa) |
| | rbfA | Probable ribosome-binding factor a RbfA (P15B protein); One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA. (183 aa) |
| | infB | Probable translation initiation factor if-2 InfB; One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity); Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. (900 aa) |
| | nusA | Probable N utilization substance protein A NusA; Participates in both transcription termination and antitermination; Belongs to the NusA family. (347 aa) |
| | rimP | Conserved protein; Required for maturation of 30S ribosomal subunits. (183 aa) |
| | proS | Proline--tRNA ligase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacy [...] (582 aa) |
| | mapB | Methionine aminopeptidase MapB (map) (peptidase M); Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed. (285 aa) |
| | rlmN | Conserved hypothetical protein; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; Belongs to the radical SAM superfamily. RlmN family. (189 aa) |
| | Rv2880c | Rv2880c, (MTCY274.11c), len: 275 aa. Conserved hypothetical protein, highly similar in N-terminus to others e.g. O86754|SC6A9.22c hypothetical 40.4 KDA protein from Streptomyces coelicolor (368 aa), FASTA scores: opt: 663, E(): 2.6e-33, (52.6% identity in 213 aa overlap); Q55880|Y098_SYNY3|SLL0098 hypothetical 38.9 KDA protein from Synechocystis sp. strain PCC 6803 (350 aa), FASTA scores: opt: 362, E(): 7.3e-15, (38.9% identity in 162 aa overlap); O66732|AQ_416 hypothetical 40.2 KDA protein from Aquifex aeolicus (348 aa), FASTA scores: opt: 321, E(): 2.4e-12, (39.75% identity in 146 aa [...] (275 aa) |
| | frr | Ribosome recycling factor Frr (ribosome releasing factor) (RRF); Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another; Belongs to the RRF family. (185 aa) |
| | tsf | Probable elongation factor Tsf (EF-ts); Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity). (271 aa) |
| | rpsB | Rv2890c, (MTCY274.21c), len: 287 aa. rpsB, 30s ribosomal protein s2, equivalent to O33038|RS2_MYCLE|RPSB|ML1598|MLCB250.63 30S ribosomal protein S2 from Mycobacterium leprae (277 aa), FASTA scores: opt: 1593, E(): 2.3e-93, (91.5% identity in 270 aa overlap). Also highly similar to others e.g. O31212|RS2_STRCO|RPSB|SC2E1.41 from Streptomyces coelicolor (310 aa), FASTA scores: opt: 1302, E(): 6.1e-75, (70.6% identity in 289 aa overlap); Q9KA63|RPSB|BH2427 from Bacillus halodurans (244 aa), FASTA scores: opt: 991, E(): 2.3e-55, (59.6% identity in 255 aa overlap); P21464|RS2_BACSU|RPSB fro [...] (287 aa) |
| | lepB | Rv2903c, (MTCY274.34c), len: 294 aa. Probable lepB,signal peptidase I (type II membrane protein) (see Braunstein & Belisle 2000), equivalent to O33021|LEP_MYCLE|ML1612|MLCB250.39 probable signal peptidase I from Mycobacterium leprae (289 aa), FASTA scores: opt: 1335, E(): 1.8e-77, (69.75% identity in 301 aa overlap). Also similar to many e.g. O86869|SIPX signal peptidase I from Streptomyces lividans (320 aa), FASTA scores: opt: 474, E(): 1e-22, (43.55% identity in 248 aa overlap); O69884|SIP1|SIPW putative signal peptidase I from Streptomyces coelicolor and Streptomyces lividans (259 a [...] (294 aa) |
| | rplS | 50S ribosomal protein L19 RplS; This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. (113 aa) |
| | trmD | tRNA (guanine-N(1)-)-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. (230 aa) |
| | rimM | Probable 16S rRNA processing protein RimM; An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes; Belongs to the RimM family. (176 aa) |
| | rpsP | Rv2909c, (MTCY274.41c), len: 162 aa. rpsP, 30S ribosomal protein S16, equivalent to O33014|RS16_MYCLE 30S ribosomal protein S16 from Mycobacterium leprae (160 aa),FASTA scores: opt: 828, E(): 1.6e-39, (82.5% identity in 160 aa overlap). Also highly similar to others e.g. O69879|RS16_STRCO 30S ribosomal protein S16 from Streptomyces coelicolor (139 aa), FASTA scores: opt: 486,E(): 1.9e-20, (56.95% identity in 144 aa overlap); P80379|RS16_THETH 30S ribosomal protein S16 from Thermus Thermophilus (88 aa), FASTA scores: opt: 280, E(): 4.8e-09,(53.25% identity in 77 aa overlap) (C-terminus [...] (162 aa) |
| | rnc | Probable ribonuclease III Rnc (RNase III); Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism (By similarity). Belongs to the ribonuclease III family. (240 aa) |
| | Rv2966c | Rv2966c, (MTCY349.21), len: 188 aa. Possible methyltransferase, equivalent (but shorter 36 aa) to O69465|MLCB1243.09 hypothetical 23.0 KDA protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 872, E(): 9.1e-50, (74.2% identity in 182 aa overlap). Also similar to others e.g. Q9ZBR2|SC7A1.11 putative methylase from Streptomyces coelicolor (195 aa), FASTA scores: opt: 510,E(): 3.7e-26, (47.5% identity in 179 aa overlap); Q9F842 hypothetical methyltransferase (fragment) from Mycobacterium smegmatis (80 aa), FASTA scores: opt: 386,E(): 2.5e-18, (75.0% identity in 80 aa overlap); P [...] (188 aa) |
| | gltS | Glutamyl-tRNA synthetase GltS (glutamate--tRNA ligase) (glutamyl-tRNA synthase) (GLURS); Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). (490 aa) |
| | gatB | Probable glutamyl-tRNA(GLN) amidotransferase (subunit B) GatB (Glu-ADT subunit B); Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity); Belongs to the GatB/GatE family. GatB subfamily. (509 aa) |
| | gatA | Probable glutamyl-tRNA(GLN) amidotransferase (subunit A) GatA (Glu-ADT subunit A); Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln) (By similarity). (494 aa) |
| | gatC | Probable glutamyl-tRNA(GLN) amidotransferase (subunit C) GatC (Glu-ADT subunit C); Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity); Belongs to the GatC family. (99 aa) |
| | trmU | Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. (367 aa) |
| | Rv3030 | Conserved protein; Probable S-adenosylmethionine-dependent methyltransferase required for the 6-O-methylation of the polysaccharide backbone of 6-O- methylglucosyl lipopolysaccharides (MGLP). (274 aa) |
| | smpB | Probable SSRA-binding protein SmpB; Required for rescue of stalled ribosomes mediated by trans- translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome th [...] (160 aa) |
| | prfB | Probable peptide chain release factor 2 PrfB (RF-2); Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. (378 aa) |
| | Rv3120 | Rv3120, (MTCY164.30), len: 200 aa. Conserved hypothetical protein, with weak similarity to several hypothetical proteins and many N-methyl transferases e.g. Q9X9V1|ORF8 putative methyltransferase from Streptomyces coelicolor A3(2) (208 aa), FASTA scores: opt: 177, E(): 0.00011, (34.6% identity in 130 aa overlap); Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa), FASTA scores: opt: 147,E(): 0.011, (31.3% identity in 166 aa overlap); BAB52127|MLL5735 probable methyltransferase from Rhizobium loti (Mesorhizobium loti) (247 aa), FASTA scores: opt: 133,E(): [...] (200 aa) |
| | sigH | Alternative RNA polymerase sigma-E factor (sigma-24) SigH (RPOE); Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by a cognate anti-sigma factor (RshA) until released. This sigma factor is involved in heat shock and oxidative stress responses; it positively regulates the expression of itself, sigE, sigB and a number of transcriptional regulators as well as other effectors of heat and oxidative stress, leading to direct and ind [...] (216 aa) |
| | sigF | Alternative RNA polymerase sigma factor SigF; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Held in an inactive form by a cognate anti-sigma factor RsbW (UsfX) until released. Increased expression decreases growth rate, and after 3 days increases the expression of 51 loci encoding 33 protein- coding genes as well as some non-coding RNA. (261 aa) |
| | Rv3300c | Rv3300c, (MTCI418A.02c), len: 305 aa. Conserved hypothetical protein, similar to various proteins (notably pseudoridine synthase family proteins) e.g. Q9RJ76|SCI41.08 putative ribosomal pseudouridine synthase from Streptomyces coelicolor (324 aa), FASTA scores: opt: 876, E(): 4.5e-48,(52.1% identity in 313 aa overlap); Q9I272|PA2043 hypothetical protein from Pseudomonas aeruginosa (300 aa),FASTA scores: opt: 676, E(): 1.8e-35, (42.55% identity in 268 aa overlap); Q9JZW8|NMB0867 YABO/YCEC/SFHB family protein from Neisseria meningitidis (serogroup B) (307 aa),FASTA scores: opt: 597, E(): [...] (305 aa) |
| | sigJ | Probable alternative RNA polymerase sigma factor (fragment) SigJ; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released, although no anti- sigma factor is known for this protein (By similarity). Regulates the promoter of SigI, may not be autoregulated. (312 aa) |
| | trpS | Probable tryptophanyl-tRNA synthetase TrpS (tryptophan--tRNA ligase) (TRPRS) (tryptophan translase); Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (336 aa) |
| | spoU | Probable tRNA/rRNA methylase SpoU (tRNA/rRNA methyltransferase); Could methylate the ribose at the nucleotide 34 wobble position in tRNA; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily. (154 aa) |
| | Rv3404c | Conserved hypothetical protein; Sugar N-formyltransferase that catalyzes the conversion of dTDP-4-amino-4,6-dideoxyglucose into dTDP-4-formamido-4,6- dideoxyglucose using N(10)-formyltetrahydrofolate as the carbon source. Plays a role in virulence. Has no activity on dTDP-3-amino-3,6-dideoxyglucose, dTDP-3-amino-3,6- dideoxygalactose, UDP-4-amino-4-deoxyarabinose, and GDP-4-amino-4,6- dideoxymannose. (234 aa) |
| | sigD | Probable alternative RNA polymerase sigma-D factor SigD; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis. (212 aa) |
| | gcp | Probable O-sialoglycoprotein endopeptidase Gcp (glycoprotease); Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. (344 aa) |
| | rimI | N-alpha-acetyltransferase RimI; N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues. Able to mediate acetylation of a wide variety of N-terminal residues, with preference for hydrophobic N- termini. Acetylates GroS/GroES and GroEL1. Able to acetylate the ribosomal protein S18, but it is unclear whether it acetylates its N- terminal alanine residue; Belongs to the acetyltransferase family. RimI subfamily. (158 aa) |
| | Rv3421c | Rv3421c, (MTCY78.08), len: 211 aa. Conserved hypothetical protein, equivalent to Q49857|YY21_MYCLE|ML0378|B229_C1_170 hypothetical 38.0 KDA protein from Mycobacterium leprae (359 aa), FASTA scores: opt: 1000, E(): 1.8e-50, (75.6% identity in 205 aa overlap). Also similar to other hypothetical bacterial proteins e.g. O86791|SC6G4.28 from Streptomyces coelicolor (217 aa), FASTA scores: opt: 453, E(): 3.3e-19, (48.1% identity in 212 aa overlap); Q9AC10|CC0059 (glycoprotease family protein) from Caulobacter crescentus (211 aa), FASTA scores: opt: 248, E(): 2e-07, (34.3% identity in 210 aa [...] (211 aa) |
| | tsaE | Conserved hypothetical protein; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD (By similarity). (168 aa) |
| | rpsI | Rv3442c, (MTCY77.14c), len: 151 aa. rpsI, 30S ribosomal protein S9, equivalent to P40828|RS9_MYCLE|ML0365|B229_C2_191 30S ribosomal protein S9 (153 aa), FASTA scores: opt: 800, E(): 2.1e-42, (83.85% identity in 155 aa overlap). Also highly similar to others e.g. Q53875|RS9_STRCO|SC6G4.13 from Streptomyces coelicolor (170 aa), FASTA scores: opt: 533, E(): 5.7e-26, (60.75% identity in 135 aa overlap); Q9KGD4|RPSI|BH0169 (BS10) from Bacillus halodurans (130 aa), FASTA scores: opt: 469, E(): 3.8e-22, (58.65% identity in 121 aa overlap); Q9CDG7|RPSI from Lactococcus lactis (subsp. lactis) ( [...] (151 aa) |
| | rplM | 50S ribosomal protein L13 RplM; This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. (147 aa) |
| | mycP4 | Mycosin-4; Rv3449, (MTCY13E12.02), len: 455 aa. Probable mycP4,membrane-anchored serine protease (mycosin) (see citation below), similar to hypothetical unknowns or proteases from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK48366|MT3998 subtilase family protein from Mycobacterium tuberculosis strain CDC1551 (411 aa), FASTA scores: opt: 747, E(): 3.5e-33, (45.65% identity in 416 aa overlap); O05461|Rv3883c|MTCY15F10.29 membrane-anchored mycosin MYCP1 (446 aa), FASTA scores: opt: 747, E(): 3.8e-33, (45.45% identity in 451 aa overlap); O53695|Rv0291|MTV035.19 probable mem [...] (455 aa) |
| | truA | tRNA pseudouridine synthase A; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. (256 aa) |
| | rplQ | Rv3456c, (MTCY13E12.09c), len: 180 aa. rplQ, 50S ribosomal protein L17, equivalent to Q9X797|RL17_MYCLE|ML1956|MLCB1222.26c 50S ribosomal protein L17 from Mycobacterium leprae (170 aa), FASTA scores: opt: 874, E(): 9.5e-45, (81.85% identity in 171 aa overlap). Also highly similar to other e.g. O86775|RL17_STRCO|SC6G4.08 from Streptomyces coelicolor (168 aa), FASTA scores: opt: 609, E(): 3.7e-29, (60.0% identity in 170 aa overlap); BAB47931|MLR0326 from Rhizobium loti (Mesorhizobium loti) (143 aa), FASTA scores: opt: 404, E(): 3.7e-17, (49.65% identity in 139 aa overlap); Q9Z9H5|RL17_TH [...] (180 aa) |
| | rpoA | DNA-directed RNA polymerase subunit alpha; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (347 aa) |
| | rpsD | 30S ribosomal protein S4 RpsD; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. Belongs to the universal ribosomal protein uS4 family. (201 aa) |
| | rpsK | 30S ribosomal protein S11 RpsK; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (139 aa) |
| | rpsM | 30S ribosomal protein S13 RpsM; Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites. Belongs to the universal ribosomal protein uS13 family. (124 aa) |
| | rpmJ | Rv3461c, (MTCY13E12.14c), len: 37 aa. rpmJ, 50S ribosomal protein L36, equivalent to P45810|RL36_MYCBO|RPMJ from Mycobacterium bovis (37 aa); and Q9X7A2|RL36_MYCLE|RPMJ|ML1961|MLCB1222.31c 50S ribosomal protein L36 from Mycobacterium leprae (37 aa), FASTA scores: opt: 241, E(): 9.7e-14, (86.5% identity in 37 aa overlap). Also highly similar to others e.g. O86772|RL36_STRCO|SC6G4.04 from Streptomyces coelicolor (37 aa), FASTA scores: opt: 233, E(): 4.5e-13, (83.8% identity in 37 aa overlap); P07841|RL36_BACST|RPMJ from Bacillus stearothermophilus (37 aa), FASTA scores: opt: 214, E(): 1. [...] (37 aa) |
| | infA | Probable translation initiation factor if-1 InfA; One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. (73 aa) |
| | Rv3579c | Rv3579c, (MTCY06G11.26c), len: 322 aa. Possible tRNA/rRNA methyltransferase, equivalent, but longer 31 aa,to Q9CCW4|ML0324 putative methyltransferase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1517,E(): 3.4e-79, (83.75% identity in 277 aa overlap). Also highly similar to Q9L0Q5|SCD8A.09 from Streptomyces coelicolor (314 aa), FASTA scores: opt: 937, E(): 3.4e-46,(56.75% identity in 319 aa overlap); and similar to others e.g. Q06753|YACO_BACSU from Bacillus subtilis (249 aa),FASTA scores: opt: 616, E(): 4.9e-28, (41.05% identity in 246 aa overlap); Q9KGF2|BH0113 from Bacillus [...] (322 aa) |
| | cysS1 | Cysteinyl-tRNA synthetase 1 CysS1 (cysteine--tRNA ligase 1) (CYSRS 1) (cysteine translase); Rv3580c, (MTCY06G11.27c), len: 469 aa. Probable cysS1, cysteinyl-tRNA synthetase, equivalent to P57990|SYC1_MYCLE|CYSS1|CYSS|ML0323 cysteinyl-tRNA synthetase 1 from Mycobacterium leprae (473 aa) FASTA scores: opt: 2825, E(): 3.4e-172, (86.5% identity in 467 aa overlap). Also similar to many e.g. Q9L0Q6|SCD8A.08 from Streptomyces coelicolor (613 aa), FASTA scores: opt: 1834,E(): 4.7e-109, (57.5% identity in 461 aa overlap); Q9I2U7|CYSS|PA1795 from Pseudomonas aeruginosa (460 aa) FASTA scores: opt [...] (469 aa) |
| | carD | Possible transcription factor; Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice; Belongs to the CarD family. (162 aa) |
| | lysS | Lysyl-tRNA synthetase 1 LysS (lysine--tRNA ligase 1) (LysRS 1) (lysine translase); Rv3598c, (MTCY07H7B.24), len: 505 aa. Probable lysS,lysyl-tRNA synthetase 1, equivalent to P46861|SYK_MYCLE|LYSS|ML0233 lysyl-tRNA synthetase from Mycobacterium leprae (507 aa), FASTA scores: opt: 2835,E(): 4.5e-172, (85.45% identity in 501 aa overlap); and similar with C-terminal part of Q9CC23|LYSX|ML1393 C-term lysyl-tRNA synthase from Mycobacterium leprae (1039 aa) FASTA scores: opt: 1257, E(): 7.6e-72, (44.55% identity in 505 aa overlap). Also similar to others e.g. P37477|SYK_BACSU|LYSS from Bacill [...] (505 aa) |
| | mesJ | Possible cell cycle protein MesJ; Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. Belongs to the tRNA(Ile)-lysidine synthase family. (323 aa) |
| | tadA | Possible cytidine/deoxycytidylate deaminase; Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2); Belongs to the cytidine and deoxycytidylate deaminase family. (152 aa) |
| | Rv3788 | Hypothetical protein; Rv3788, (MTCY13D12.22), len: 161 aa. Hypothetical unknown protein. (161 aa) |
| | Rv3796 | Conserved protein; Rv3796, (MTV026.01-MTCY13D12.30), len: 375 aa. Conserved protein. C-terminal end similar in part to Q983J3|MLR8305 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (227 aa), FASTA scores: opt: 288, E(): 4e-09, (38.95% identity in 154 aa overlap). Similar to P54548|YQJK_BACSU hypothetical protein (belongs to the ATSA/ELAC family) from Bacillus subtilis (307 aa) FASTA scores: opt: 263, E(): 1.3e-07, (26.1% identity in 295 aa overlap); and some similarity to other proteins e.g. AAK46775|MT2479 putative arylsulfatase from Mycobacterium tuberculosis strain CD [...] (375 aa) |
| | serS | SERYL-tRNA synthetase SerS (serine--tRNA ligase) (SERRS) (serine translase); Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (419 aa) |
| | mycP1 | Mycosin-1; May play a dual role in regulation of ESX-1 secretion and virulence. Acts as a protease that cleaves EspB. Essential for ESX-1 function, required for early replication in macrophages and full virulence in mice; Belongs to the peptidase S8 family. (446 aa) |
| | mycP2 | Mycosin-2; Rv3886c, (MTCY15F10.26), len: 550 aa. Probable mycP2, ala-, pro-rich membrane-anchored serine protease (mycosin) (see citation below), highly similar to Q9CBV3|ML1538 possible protease from Mycobacterium leprae (567 aa), FASTA scores: opt: 1034, E(): 3.9e-32, (43.5% identity in 575 aa overlap); and highly similar, but with gaps, to several putative proteases from Mycobacterium leprae; O33076|ML0041|MLCB628.04 (446 aa), FASTA scores: opt: 860, E(): 1.1e-25, (38.65% identity in 538 aa overlap); Q9CD36|ML2528 (475 aa) (475 aa), FASTA scores: opt: 413, E(): 7.1e-09, (37.7% ident [...] (550 aa) |
| | pcnA | Poly(A) polymerase PcnA; Rv3907c, (MTCY15F10.04), len: 480 aa. Probable pcnA,polynucleotide polymerase, equivalent to Q9CCY1|PCNA|ML2697 PCNA protein from Mycobacterium leprae (486 aa), FASTA scores: opt: 2713, E(): 4.3e-160, (84.1% identity in 478 aa overlap); and Q59534|PCNB POLYA polymerase from Mycobacterium leprae (411 aa) FASTA scores: opt: 2077, E(): 7.1e-121, (82.55% identity in 373 aa overlap). Also highly similar to many e.g. Q9X8T2|SCH24.18 putative RNA nucleotidyltransferase from Streptomyces coelicolor (483 aa), FASTA scores: opt: 1856, E(): 3.7e-107, (61.55% identity in 4 [...] (480 aa) |
| | sigM | Possible alternative RNA polymerase sigma factor SigM; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor (RsaM, AC L7N5D7) until released by regulated intramembrane proteolysis (Probable). This sigma factor is required for the synthesis of surface or secreted molecules. (222 aa) |
| | gid | Probable glucose-inhibited division protein B Gid; Specifically methylates the N7 position of guanine in position 518 of 16S rRNA. (224 aa) |
| | rnpA | Ribonuclease P protein component RnpA (RNaseP protein) (RNase P protein) (protein C5); RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. Belongs to the RnpA family. (125 aa) |
| | rpmH | Rv3924c, (MTV028.15), len: 47 aa. rpmH, 50s ribosomal protein l34 (see citations below), equivalent to many mycobacterial 50S ribosomal protein L34 e.g. P46386|RL34_MYCLE|RPMH|ML2713 from Mycobacterium leprae (47 aa), FASTA scores: opt: 287, E(): 8.5e-17, (91.5% identity in 47 aa overlap); and Q9L7L8|RL34_MYCPA|RPMH from Mycobacterium paratuberculosis (47 aa), FASTA scores: opt: 281, E(): 2.6e-16, (89.35% identity in 47 aa overlap). Also highly similar to other ribosomal proteins e.g. P27901|RL34_STRCO|RPMH|STH24.02 from Streptomyces coelicolor (45 aa), FASTA scores: opt: 234, E(): 1.4 [...] (47 aa) |
