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| | thiD | Probable phosphomethylpyrimidine kinase ThiD (HMP-phosphate kinase) (HMP-P kinase); Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Belongs to the ThiD family. (265 aa) |
| | pknG | Serine/threonine-protein kinase PknG (protein kinase G) (STPK G); Phosphorylates GarA. May play a role in metabolic regulation via control of the phosphorylation status of GarA. Plays a crucial role in the survival of mycobacteria within host macrophages, by blocking the intracellular degradation of mycobacteria in lysosomes. Required for intrinsic antibiotic resistance. (750 aa) |
| | ackA | Probable acetate kinase AckA (acetokinase); Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction; Belongs to the acetokinase family. (385 aa) |
| | fba | Probable fructose-bisphosphate aldolase Fba; Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. (344 aa) |
| | dnaK | Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70); Acts as a chaperone; Belongs to the heat shock protein 70 family. (625 aa) |
| | cobU | Rv0254c, (MTV034.20), len: 174 aa. Probable cobU,cobalamin biosynthesis protein including a cobinamide kinase and cobinamide phosphate guanylyltransferase. Highly similar to many e.g. Q05599|COBU_SALTY cobinamide kinase / cobinamide phosphate guanylyltransferase from Salmonella typhimurium (181 aa), FASTA scores: opt: 308, E(): 1.1e-14,(38.7% identity in 181 aa overlap); P46886|COBU_ECOLI|B1993|Z3153|ECS2788 Bifunctional cobalamin biosynthesis protein cobU from Escherichia coli strains K12 and O157:H7 (181 aa); part of AL096872|SC5F7_10 from Streptomyces coelicolor (397 aa), FASTA scor [...] (174 aa) |
| | mak | Maltokinase Mak; Catalyzes the ATP-dependent phosphorylation of maltose to maltose 1-phosphate (By similarity). Is involved in a branched alpha- glucan biosynthetic pathway from trehalose, together with TreS, GlgE and GlgB; Belongs to the aminoglycoside phosphotransferase family. (455 aa) |
| | hddA | Rv0115, (MTV031.09), len: 386 aa. Possible hddA,D-alpha-D-heptose-7-phosphate kinase (see citation below),similar to several hypothetical proteins and sugar kinases e.g. AAK27850.1|AF324836_3 D-glycero-D-manno-heptose 7-phosphate kinase from Aneurinibacillus thermoaerophilus (341 aa); AAK80995.1|AE007802_11 Sugar kinase from Clostridium acetobutylicum (364 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (386 aa) |
| | pknA | Transmembrane serine/threonine-protein kinase A PknA (protein kinase A) (STPK A); Protein kinase that regulates many aspects of mycobacterial physiology, and is critical for growth in vitro and survival of the pathogen in the host. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins such as FtsZ, Wag31, GlmU, FhaB, PstP, EmbR and Rv1422. Also catalyzes the phosphorylation of the proteasome alpha-subunit (PrcA) and unprocessed proteasome beta-subunit (pre-PrcB), which results in the inhibitio [...] (431 aa) |
| | pknB | Transmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B); Protein kinase that regulates many aspects of mycobacterial physiology, and is critical for growth in vitro and survival of the pathogen in the host. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins such as GarA, GlmU, PapA5, PbpA, FhaB (Rv0019c), FhaA (Rv0020c), MviN, PstP, EmbR, Rv1422, Rv1747 and RseA. Also catalyzes the phosphorylation of the core proteasome alpha-subunit (PrcA), and thereby regulates th [...] (626 aa) |
| | xylB | Rv0729, (MTV041.03), len: 448 aa. Possible xylB,D-xylulose-kinase (xylulokinase). C-terminus highly similar to AAD09880.1|U77912 unknown protein from Mycobacterium bovis (102 aa); and N-terminus highly similar to T45387|Z98756|MLCB2492_25 hypothetical protein from Mycobacterium leprae (110 aa), FASTA scores: opt: 427, E(): 1.1e-19, (60.9% identity in 110 aa overlap). Also similar to xylA/xylB genes from various bacterial species e.g. AAC26499.1|AF045245 D-xylulose-kinase from Klebsiella pneumoniae (487 aa); NP_418021.1|NC_000913 xylulokinase from Escherichia coli strain K12 (484 aa), F [...] (448 aa) |
| | Rv0650 | Possible sugar kinase; Rv0650, (MTCY20H10.31), len: 302 aa. Possible sugar kinase, highly similar to others e.g. CAB95296.1|AL359779 putative sugar kinase from Streptomyces coelicolor (317 aa); NP_406512.1|NC_003143 putative sugar kinase from Yersinia pestis (290 aa); NP_229269.1|NC_000853 glucokinase from Thermotoga maritima (317 aa); etc.Contains PS01125 ROK family signature. Belongs to the ROK (NAGC/XYLR) family. (302 aa) |
| | Rv0647c | Conserved protein; Rv0647c, (MTCY20H10.28c), len: 488 aa. Conserved protein, equivalent to NP_302277.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (448 aa). Also showing similarity to a variety of hypothetical ABC1-like proteins or conserved hypothetical proteins e.g. D90908_28|P73627 ABC1-like protein from Synechocystis (585 aa), FASTA scores: E(): 1.8e-31, (29.1% identity in 474 aa overlap); Q55884 HYPOTHETICAL6 5.0 KD protein (567 aa),FASTA scores: opt: 583, E(): 5.7e-30, (28.1% identity in 416 aa overlap); etc. Also similar to Rv3197 conserved hypothetical pr [...] (488 aa) |
| | galK | Probable galactokinase GalK (galactose kinase); Catalyzes the transfer of the gamma-phosphate of ATP to D- galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). (363 aa) |
| | adk | Adenylate kinase Adk (ATP-AMP transphosphorylase); Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Has a broad specificity for nucleoside triphosphates, being highly active with ATP or dATP as phosphate donors, and less active with GTP or UTP. (181 aa) |
| | Rv0601c | Two component sensor kinase [first part]; Member of the three-protein two-component system HK1/HK2/TcrA. HK2 transfers its phosphoryl group to TcrA. (156 aa) |
| | Rv0600c | Two component sensor kinase [second part]; Member of the three-protein two-component system HK1/HK2/TcrA. Kinase that binds ATP and catalyzes the transfer of a phosphoryl group from ATP to HK2. (168 aa) |
| | senX3 | Putative two component sensor histidine kinase SenX3; Probably forms part of a two-component regulatory system SenX3/RegX3. Phosphorylates RegX3 (Probable). (410 aa) |
| | gpm1 | Probable phosphoglycerate mutase 1 Gpm1 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM); Catalyzes the interconversion of 2-phosphoglycerate and 3- phosphoglycerate; Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. (249 aa) |
| | lpdC | Dihydrolipoyl dehydrogenase; Lipoamide dehydrogenase is an essential component of the alpha-ketoacid dehydrogenase complexes, namely the pyruvate dehydrogenase (PDH) complex, the branched-chain alpha-ketoacid dehydrogenase (BCKADH) complex, and likely also the 2-oxoglutarate dehydrogenase (ODH) complex. Catalyzes the reoxidation of dihydrolipoyl groups which are covalently attached to the lipoate acyltransferase components (E2) of the complexes. Is also able to catalyze the transhydrogenation of NADH and thio-NAD(+) in the absence of D,L- lipoamide, and the NADH-dependent reduction of [...] (464 aa) |
| | Rv3817 | Rv3817, (MTCY409.13c), len: 251 aa. Possible phosphotransferase, similar to many phosphotransferases e.g. O53023 kanamycin marker from Escherichia coli (264 aa), FASTA scores: opt: 232, E(): 7.5e-08, (32.4% identity in 247 aa overlap); BAA78209|NEO neomycine phosphotransferase from Drosophila melanogaster (Fruit fly) (264 aa), FASTA scores: opt: 227, E(): 1.6e-07, (32.0% identity in 247 aa overlap); AAG09774 aminoglycoside 3'-phosphotransferase from Vibrio cholerae (264 aa), FASTA scores: opt: 227, E(): 1.6e-07, (32.0% identity in 247 aa overlap); P00552|KKA2_KLEPN|NEO|KAN aminoglycosi [...] (251 aa) |
| | tcrY | Possible two component sensor kinase TcrY; Member of the two-component regulatory system TcrY/TcrX. Activates TcrX by phosphorylation. (475 aa) |
| | ask | Aspartokinase Ask (aspartate kinase) [contains: aspartokinase alpha subunit (Ask-alpha); Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids lysine, threonine, isoleucine and methionine; Belongs to the aspartokinase family. (421 aa) |
| | Rv3705c | Conserved protein; Rv3705c, (MTV025.053c), len: 214 aa. Conserved protein, equivalent to Q9CB80|ML2320 hypothetical protein from Mycobacterium leprae (215 aa) FASTA scores: opt: 1145,E(): 5.9e-68, (79.45% identity in 214 aa overlap). Some similarity to the C-terminal end of Q11053|PKNH_MYCTU|Rv1266c|MT1304|MTCY50.16 probable serine/threonine-protein from Mycobacterium tuberculosis (626 aa), FASTA scores: opt: 175, E(): 0.0005, (24.9% identity in 201 aa overlap); and to the N-terminal end of P23903|E13B_BACCI|GLCA glucan endo-1,3-beta-glucosidase A1 precursor from Bacillus circulans (68 [...] (214 aa) |
| | glpK | Probable glycerol kinase GlpK (ATP:glycerol 3-phosphotransferase) (glycerokinase) (GK); Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn- glycerol 3-phosphate; Belongs to the FGGY kinase family. (517 aa) |
| | folK | Rv3606c, (MTCY07H7B.16), len: 188 aa. Probable folK,2-amino-4-hydroxy-6-hydroxymethyldihydropterine pyrophosphokinase, equivalent to O69528|HPPK_MYCLE|folk|ML0226MLCB2548.05c 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase from Mycobacterium leprae (191 aa) FASTA scores: opt: 772, E(): 1.2e-44, (63.15% identity in 190 aa overlap). Also similar to many e.g. P71512|HPPK_METEX|folk|FOLA from Methylobacterium extorquens (158 aa), FASTA scores: opt: 292, E(): 1.4e-12,(36.85% identity in 171 aa overlap); O33726|HPPK_STRPY|folk|SPY1100 from Streptococcus pyogenes (166 aa) [...] (188 aa) |
| | coaX | Conserved protein; Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis; Belongs to the type III pantothenate kinase family. (272 aa) |
| | lppH | Rv3576, (MTCY06G11.23), len: 237 aa. Possible lppH,conserved lipoprotein, similar in part with proteins from Mycobacterium tuberculosis; C-terminus of Q11053|PKNH_MYCTU|PKNH|Rv1266c|MT1304|MTCY50.16 probable serine/threonine-protein kinase (626 aa) FASTA scores: opt: 396, E(): 6.5e-19, (36.0% identity in 200 aa overlap); and with P71740|LPPR|Rv2403c|MTCY253.17 probable lipoprotein protein (251 aa), FASTA scores: opt: 134, E(): 0.087,(22.7% identity in 207 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as pknM. (237 aa) |
| | Rv3365c | Conserved protein; Rv3365c, (MTV004.22c), len: 876 aa. Conserved protein, similar to various proteins from Streptomyces coelicolor e.g. O86525|SC1C2.25c hypothetical 139.7 KDA protein (similar to other prokaryotic sensory transduction histidine kinases) (1329 aa), FASTA scores: opt: 879, E(): 5.4e-32, (29.9% identity in 924 aa overlap) (similarity in N-terminal part for this one); O86522|SC1C2.21c hypothetical 119.9 KDA protein (similar to other prokaryotic sensory transduction histidine kinases) (1111 aa), FASTA scores: opt: 855, E(): 5.6e-31, (28.9% identity in 892 aa overlap) (simil [...] (876 aa) |
| | tmk | Thymidylate kinase Tmk (dTMP kinase) (thymidylic acid kinase) (TMPK); Catalyzes the reversible phosphorylation of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP), using ATP as its preferred phosphoryl donor. Situated at the junction of both de novo and salvage pathways of deoxythymidine triphosphate (dTTP) synthesis, is essential for DNA synthesis and cellular growth. Has a broad specificity for nucleoside triphosphates, being highly active with ATP or dATP as phosphate donors, and less active with ITP, GTP, CTP and UTP; Belongs to the thymidylate kinase family. (214 aa) |
| | mtrA | Two component sensory transduction transcriptional regulatory protein MtrA; Member of the two-component regulatory system MtrA/MtrB. Binds direct repeat motifs of sequence 5'-GTCACAGCG-3', phosphorylation confers higher affinity. Overexpression decreases bacteria viability upon infection of human THP-1 macrophage cell line, due at least in part to impaired blockage of phagosome-lysosome fusion (upon infection bacteria usually remain in phagosomes). Infecting C57BL/6 mice with an overexpressing strain leads to an attentuated infection in both spleen and lungs. The level of dnaA mRNA inc [...] (228 aa) |
| | mtrB | Two component sensory transduction histidine kinase MtrB; Member of the two-component regulatory system MtrA/MtrB. Probably functions as a membrane-associated protein kinase that phosphorylates MtrA in response to environmental signals. Autophosphorylates and transfers phosphate to MtrA in vitro. Overexpression of MtrA alone decreases bacterial virulence in mouse infection; co-expression of MtrA and MtrB restores normal bacterial growth, suggesting that bacterial growth in macrophages requires an optimal ratio of MtrB to MtrA. Probably plays a role in cell division. (567 aa) |
| | ppk2 | Polyphosphate kinase Ppk2 (polyphosphoric acid kinase); Uses inorganic polyphosphate (polyP) as a donor to convert GDP to GTP. In addition, modulates nucleotide triphosphate synthesis catalyzed by the nucleoside diphosphate kinase (Ndk) in favor of GTP production over CTP or UTP. Plays an important role in survival of M.tuberculosis in macrophages. (295 aa) |
| | pdtaS | Probable two component sensor kinase; Member of the two-component regulatory system PdtaR/PdtaS. Autophosphorylates, probably on a histidine residue, and transfers its phosphate group to PdtaR. (501 aa) |
| | Rv3168 | Putative aminoglycoside phosphotransferase; Might catalyze the phosphorylation of aminoglycosides and confer aminoglycoside antibiotics resistance. (378 aa) |
| | devS | Two component sensor histidine kinase DevS; Member of the two-component regulatory system DevR/DevS (DosR/DosS) involved in onset of the dormancy response. Regulates an approximately 48-member regulon. Required for full induction of the DevR (DosR) regulon; acts later than DosT to positively regulate expression of the DevR regulon during adaptation to anaerobiosis. Characterized as an oxygen sensor; O(2) acts as a switch, with O(2)-bound Fe(2+) protein inactive in autophosphorylation. Has also been suggested to act as a redox sensor, or perhaps as a dual oxygen/redox sensor. Autophosph [...] (578 aa) |
| | pknK | Serine/threonine-protein kinase transcriptional regulatory protein PknK (protein kinase K) (STPK K); Key microbial factor involved in regulation of early and late events in tuberculosis infection, and in host-pathogen interactions. Modulates host immunity during early infection. Slows mycobacterial growth during chronic infection in host and during a variety of stress conditions in vitro. Regulates the expression of a large subset of tRNA genes as a means to facilitate adaptation to changing growth environments. In vitro, directs the inhibition of transcription and translation processe [...] (1110 aa) |
| | lpqA | Rv3016, (MTV012.30), len: 209 aa. Probable lpqA,lipoprotein. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. (209 aa) |
| | pfkA | Probable 6-phosphofructokinase PfkA (phosphohexokinase) (phosphofructokinase); Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis; Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily. (343 aa) |
| | Rv2998A | Rv2998A, len: 67 aa. Probable conserved hypothetical protein, (possibly gene fragment), highly similar to central part of two-component sensor proteins e.g. O07777|Rv0601c|MTCY19H5.21 two component sensor (fragment) from Mycobacterium tuberculosis (156 aa), FASTA scores: opt: 212, E(): 3.7e-09, (58.2% identity in 67 aa overlap); Q9L2B6|SC8F4.08 probable two-component sensor kinase from Streptomyces coelicolor (478 aa), FASTA scores: opt: 193,E(): 2.6e-07, (47.05% identity in 68 aa overlap); etc. (67 aa) |
| | ppk1 | Polyphosphate kinase PPK (polyphosphoric acid kinase) (ATP-polyphosphate phosphotransferase); Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP); Belongs to the polyphosphate kinase 1 (PPK1) family. (742 aa) |
| | thiL | Probable thiamine-monophosphate kinase ThiL (thiamine-phosphate kinase); Catalyzes the ATP-dependent phosphorylation of thiamine- monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1. (333 aa) |
| | pknI | Serine/threonine-protein kinase PknI; Plays an important role in slowing down the growth of mycobacteria within the infected host; Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. (585 aa) |
| | pyrH | Probable uridylate kinase PyrH (UK) (uridine monophosphate kinase) (UMP kinase); Catalyzes the reversible phosphorylation of UMP to UDP. (261 aa) |
| | ribF | Riboflavin biosynthesis protein; Rv2786c, (MTV002.51c), len: 331 aa. Probable ribF,FAD synthetase/riboflavin biosynthesis protein,bifunctional enzyme, equivalent to O32968|RIBF|ML0852 riboflavin kinase from Mycobacterium leprae (331 aa), FASTA scores: opt: 1923, E(): 2.3e-115, (87.45% identity in 327 aa overlap). Also highly similar to many e.g. Q59263|RIBF_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (338 aa), FASTA scores: opt: 899, E(): 5.7e-50, (45.8% identity in 321 aa overlap); Q9Z530|SC9F2.05c from Streptomyces coelicolor (318 aa),FASTA scores: opt: 862, [...] (331 aa) |
| | ppgK | Polyphosphate glucokinase PpgK (polyphosphate-glucose phosphotransferase); Catalyzes the phosphorylation of glucose using polyphosphate or ATP as the phosphoryl donor. Polyphosphate, rather than ATP, seems to be the major phosphate donor for the enzyme in M.tuberculosis (By similarity); Belongs to the ROK (NagC/XylR) family. (265 aa) |
| | relA | Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase; In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the formation of pppGpp, which is then hydrolyzed to form ppGpp, as well as the hydrolysis of ppGpp. RelA is probably a key factor in the pathogenesis of M.tuberculosis as it regulates the intracellular concentrations of (p)ppGpp. (790 aa) |
| | aroK | Shikimate kinase AroK (SK); Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. Belongs to the shikimate kinase family. (176 aa) |
| | ndkA | Probable nucleoside diphosphate kinase NdkA (NDK) (NDP kinase) (nucleoside-2-P kinase); Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate; Belongs to the NDK family. (136 aa) |
| | obg | Probable GTP1/Obg-family GTP-binding protein Obg; An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control (By similarity). GTPase activity is not inhibited by ATP or GDP. Overexpression decreases cell growth starting in late log phase and continuing into stationary phase ; Belongs to the TRAFAC class OBG-HflX-like GTPase superfa [...] (479 aa) |
| | proB | Probable glutamate 5-kinase protein ProB (gamma-glutamyl kinase) (GK); Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate. (376 aa) |
| | rbsK | Ribokinase RbsK; Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway. (304 aa) |
| | lppR | Rv2403c, (MTCY253.17), len: 251 aa. Probable lppR,conserved lipoprotein, with weak similarity with mycobacterial serine/threonine protein kinases e.g. AAK45563|MT1304 from Mycobacterium tuberculosis strain CDC1551 (626 aa), FASTA scores: opt: 186, E(): 0.00023,(24.4% identity in 238 aa overlap), and the C-terminal part of Q11053|Rv1266c|MTCY50.16|PKNH_MYCTU from Mycobacterium tuberculosis (626 aa), FASTA scores: opt: 185, E()= 0.00027, (24.35% identity in 238 aa overlap). Has signal peptide and appropriate positioned prokaryotic lipoprotein attachment site (PS00013). Could belong to th [...] (251 aa) |
| | dagK | Diacylglycerol kinase; Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derived from phosphatidic acid (By similarity); Belongs to the diacylglycerol/lipid kinase family. (309 aa) |
| | aceE | Pyruvate dehydrogenase E1 component; Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). AceE has reductase activity with pyruvate but does not react with 2- oxoglutarate. (901 aa) |
| | ptkA | Rv2232, (MTCY427.13), len: 291 aa. PtkA, protein tyrosine kinase, similar to members of haloacid dehalogenase-like family from several bacteria and to putative phosphatases e.g. Q9I767 and AAK78398. Contains N-terminal extension. FASTA scores: Q9I767 hypothetical protein PA0065 (221 aa) opt: 439 E(): 3.2e-18; 38.679% identity (40.196% ungapped) in 212 aa overlap; >>tr|AAK78398 Predicted phosphatase, had family (216 aa) opt: 427, E(): 1.5e-17; 34.762% identity (35.437% ungapped) in 210 aa overlap. Replaces previous Rv2232 and Rv2233. Predicted to be an outer membrane protein (See Song e [...] (291 aa) |
| | dlaT | DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase; Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). Appears to be essential for Mtb pathogenesis. (553 aa) |
| | Rv2205c | Rv2205c, (MTCY190.16c), len: 358 aa. Conserved hypothetical protein. Very similar to YHAD_ECOLI|P23524 hypothetical protein (YHAD (E.coli) / YXAA (S14A) (B.subtilis) family) (41.6% identity in 154 aa overlap),and to other members of the glycerate kinase family. Start changed since first submission; protein now 122 aa shorter,owing to extension of Rv2206. Nucleotide position 2470149 in the genome sequence has been corrected, T:C resulting in E105E. (358 aa) |
| | adoK | Adenosine kinase; Catalyzes the phosphorylation of adenosine to adenosine monophosphate (AMP). Can also catalyze the phosphorylation of the adenosine analog 2-methyladenosine (methyl-Ado) to methyl-AMP, the first step in the metabolism of this compound to an active form that displays antitubercular activity. Is not active on guanosine, inosine, deoxyadenosine, cytidine, uridine, or thymidine. Prefers dGTP and GTP to ATP as phosphate donors in vitro. (324 aa) |
| | pknL | Probable transmembrane serine/threonine-protein kinase L PknL (protein kinase L) (STPK L); Phosphorylates the DNA-binding protein Rv2175c. May be involved in the regulation of cell division and cell envelope biosynthesis. (399 aa) |
| | Rv2175c | Conserved regulatory protein; Binds DNA at low salt concentrations. (146 aa) |
| | pknJ | Transmembrane serine/threonine-protein kinase J PknJ (protein kinase J) (STPK J); In vitro, phosphorylates various substrates such as EmbR, PepE, MmaA4, Pyk, LldD and GroEL2. (589 aa) |
| | Rv2047c | Rv2047c, (MTV018.34c), len: 854 aa. Conserved hypothetical protein, similar to many. Contains IPR016040 NAD(P)-binding domain at N-terminus; Belongs to the PEP-utilizing enzyme family. (854 aa) |
| | hspX | Heat shock protein HspX (alpha-crystallin homolog) (14 kDa antigen) (HSP16.3); Acts as a chaperone, as it has a significant ability to suppress the thermal denaturation of alcohol dehydrogenase. Cells overexpressing this gene grow more slowly than wild-type cells, and are less susceptible to autolysis following saturation of the culture in vitro, suggesting this protein may slow down the growth rate of M.tuberculosis in culture and by extension during macrophage infection. Belongs to the small heat shock protein (HSP20) family. (144 aa) |
| | pfkB | 6-phosphofructokinase PfkB (phosphohexokinase) (phosphofructokinase); Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. (339 aa) |
| | dosT | Two component sensor histidine kinase DosT; Interacts with the two-component regulatory system DevR/DevS (DosR/DosS) involved in onset of the dormancy response. Required for full induction of the DevR (DosR) regulon; required during early adaptation to anaerobiosis, to start induction of the DevR regulon. May act as a direct hypoxia/oxygen sensor. O(2) acts as a switch, with the Fe(2+)-O(2)-bound protein inactive in autophosphorylation. Autophosphorylates under anaerobic but not aerobic conditions, binding of NO or CO has no effect on autophosphorylation. Binds a number of gases; O(2), [...] (573 aa) |
| | garA | Conserved protein with FHA domain, GarA; Involved in regulation of glutamate metabolism. Acts as a phosphorylation-dependent molecular switch that modulates the activities of Kgd, Gdh and GltB. (162 aa) |
| | pknF | Anchored-membrane serine/threonine-protein kinase PknF (protein kinase F) (STPK F); Phosphorylates the FHA domains of the ABC transporter Rv1747, the heat-shock protein GroEL 1, and Rv0020c. May play a role in the regulation of glucose transport, cell growth and septum formation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. (476 aa) |
| | pknE | Probable transmembrane serine/threonine-protein kinase E PknE (protein kinase E) (STPK E); Important for survival of the bacterium in the host during infection. Promotes the survival of infected macrophages by activating multiple signaling responses and suppressing apoptosis of macrophages during nitrate stress. May contribute to the adaptation of M.tuberculosis during stress conditions by maintaining the cellular integrity. Can phosphorylate the FHA domain of Rv1747. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. (566 aa) |
| | cmk | Cytidylate kinase Cmk (CMP kinase) (cytidine monophosphate kinase) (ck); Rv1712, (MTCI125.34), len: 230 aa. cmk, cytidylate kinase, highly similar to many e.g. KCY_ECOLI|P23863 cytidylate kinase from Escherichia coli (227 aa), FASTA scores: opt: 534, E (): 0, (40.3% identity in 221 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Equivalent to Z95117|MLCB1351_2 from Mycobacterium leprae (223 aa) (73.5% identity in 226 aa overlap). Belongs to the cytidylate kinase family,subfamily 1. (230 aa) |
| | Rv1697 | Rv1697, (MTCI125.19), len: 393 aa. Conserved hypothetical protein, highly similar to Q49895|MLC1351.11C|U00021 Hypothetical protein of Mycobacterium leprae from cosmid L247 (430 aa), FASTA scores: opt: 2345, E(): 0, (90.6% identity in 393 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (393 aa) |
| | ppnK | Inorganic polyphosphate/ATP-NAD kinase PpnK (poly(P)/ATP NAD kinase); Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. It can use ATP and other nucleoside triphosphates as well as inorganic polyphosphate (poly(P)) as a source of phosphorus. (307 aa) |
| | argB | Probable acetylglutamate kinase ArgB; Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate. (294 aa) |
| | coaE | Probable dephospho-CoA kinase CoaE (dephosphocoenzyme a kinase); Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A. Can also use dATP, with lower efficiency, but cannot use GTP, dGTP or CTP ; In the C-terminal section; belongs to the UPF0157 (GrpB) family. (407 aa) |
| | pykA | Rv1617, (MTCY01B2.09), len: 472 aa. Probable pykA,pyruvate kinase. FASTA best: Q46078 pyruvate kinase from corynebacterium glutamicum (475 aa), opt: 2221, E(): 0,(72.2% identity in 468 aa overlap). Belongs to the pyruvate kinase family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). (472 aa) |
| | tpi | Probable triosephosphate isomerase Tpi (TIM); Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D- glyceraldehyde-3-phosphate (G3P); Belongs to the triosephosphate isomerase family. (261 aa) |
| | pgk | Rv1437, (MTCY493.17c), len: 412 aa. Probable pgk,Phosphoglycerate kinase, highly similar to many e.g. PGK_MYCLE|P46712 Mycobacterium leprae (416 aa), FASTA scores: opt: 2153, E(): 0, (80.4% identity in 414 aa overlap). Contains PS00111 Phosphoglycerate kinase signature. Belongs to the phosphoglycerate kinase family. (412 aa) |
| | gap | Probable glyceraldehyde 3-phosphate dehydrogenase Gap (GAPDH); Catalyzes the oxidative phosphorylation of glyceraldehyde 3- phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG; Belongs to the glyceraldehyde-3-ph [...] (339 aa) |
| | gmk | Probable guanylate kinase Gmk; Essential for recycling GMP and indirectly, cGMP. (208 aa) |
| | Rv1364c | Rv1364c, (MTCY02B10.28c), len: 653 aa. Possible sigma factor regulatory protein, some similarity to RSBU_BACSU|P40399 sigma factor sibg regulation protein from Bacillus subtilis (335 aa), FASTA scores: opt: 224, E(): 2e-07, (25.8% identity in 244 aa overlap). Also known as mursiF. (653 aa) |
| | thrB | Probable homoserine kinase ThrB; Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate. (316 aa) |
| | cysN | Sulfate adenylyltransferase subunit 1; ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity; In the C-terminal section; belongs to the APS kinase family. (614 aa) |
| | pknH | Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H); May regulate bacterial growth in response to external signals to facilitate adaptation to the host environment. In vitro, phosphorylates several substrates such as EmbR, DevR (DosR), DacB1 and Rv0681. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. (626 aa) |
| | ppdK | Rv1127c, (MTCY22G8.16c), len: 490 aa. Probable ppdK,Pyruvate, phosphate dikinase. Equivalent (but shorter) to Z94723|MLCB33_16 ppdK from Mycobacterium leprae (601 aa) (71.8% identity in 478 aa overlap). Highly similar to N-terminus of PODK_CLOSY|P22983 pyruvate, phosphate dikinase from Clostridium symbiosum (873 aa), FASTA scores: opt: 786, E(): 0, (37.4% identity in 514 aa overlap). (490 aa) |
| | coaA | Rv1092c, (MTV017.45c), len: 312 aa. Probable coaA,pantothenate kinase, similar to many e.g. P15044|COAA_ECOLI Escherichia coli (316 aa), FASTA scores :opt: 1079, E(): 0,(52.7% identity in 311 aa overlap). Equivalent to AL049491|MLCB1222_17 Mycobacterium leprae (312 aa) (93.6% identity in 312 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the pantothenate kinase family. (312 aa) |
| | trcS | Rv1032c, (MTCY10G2.17), len: 509 aa. TrcS, two component sensor histidine kinase protein (see citations below), similar to YV16_MYCLE|P54883 probable sensor-like histidine kinase from Mycobacterium leprae (443 aa), FASTA scores: opt: 392, E(): 3.8e-18, (31.7% identity in 334 aa overlap). Note that in vitro autophosphorylation of TrcS requires the presence of Mn2+ or Ca2+ as a divalent cation cofactor and subsequent transphosphorylation of TrcR is evident in the presence of TrcS-phosphate and Ca2+. (509 aa) |
| | kdpD | Probable sensor protein KdpD; Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of KdpE in response to environmental signals such as low levels of potassium ion, osmotic imbalance, acid and nutrient stresses. In turn, KdpE binds to the upstream promoter regions of target genes to positively regulate their expression. (860 aa) |
| | eno | Probable enolase Eno; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis; Belongs to the enolase family. (429 aa) |
| | prsA | Ribose-phosphate pyrophosphokinase; Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) and of the decaprenylphosphoryl-arabinose (DPA), an essential precursor for the mycobacterial cell wall biosynthesis. Catalyzes the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P) to yield phosphoribosyl diphosphate (PRPP) and AMP. It can also use GTP, CTP and UTP as diphosphoryl donors. (326 aa) |
| | ispE | 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol; Belongs to the GHMP kinase family. IspE subfamily. (306 aa) |
| | mprB | Two component sensor kinase MprB; Member of the two-component regulatory system MprB/MprA which contributes to maintaining a balance among several systems involved in stress resistance and is required for establishment and maintenance of persistent infection in the host. In response to environmental signals MprB acts as both a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to MprA, and a protein phosphatase that dephosphorylates phospho-MprA. MprB/MprA is involved in regulation of numerous stress-responsive genes, includin [...] (504 aa) |
| | pgi | Glucose-6-phosphate isomerase; Rv0946c, (MTCY10D7.28), len: 553 aa. Probable pgi,glucose-6-phosphate isomerase, equivalent to NP_301236.1|NC_002677 glucose-6-phosphate isomerase from Mycobacterium leprae (554 aa); and P96803|G6PI_MYCSM glucose-6-phosphate isomerase from Mycobacterium smegmatis (442 aa). Also highly similar to others e.g. T36015 glucose-6-phosphate isomerase from Streptomyces coelicolor (551 aa); P11537|G6PI_ECOLI|GPI glucose-6-phosphate isomerase from Escherichia coli strains K12 and O157:H7 (549 aa), FASTA scores: opt: 1779, E(): 0, (51.4% identity in 554 aa overlap); [...] (553 aa) |
| | pknD | Transmembrane serine/threonine-protein kinase D PknD (protein kinase D) (STPK D); Key microbial factor required for central nervous system tuberculosis. Required for invasion of host brain endothelia, but not macrophages, lung epithelia or other endothelia. Phosphorylates the anti-anti-sigma factor homolog Rv0516c, which inhibits binding of Rv0516c to Rv2638, another anti-anti-sigma factor. Can also phosphorylate the FHA domain of Rv1747. (664 aa) |
| | prrB | Two component sensor histidine kinase PrrB; Member of the two-component regulatory system PrrB/PrrA that is involved specifically in early intracellular multiplication of Mycobacterium and is essential for its viability. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of PrrA. In turn, PrrA binds to the upstream promoter regions of target genes including itself to positively regulate their expression. (446 aa) |
| | narS | Possible two component sensor kinase; Member of the two-component regulatory system NarS/NarL involved in gene expression during aerobic nitrate metabolism. Plays therefore a crucial role in anaerobic survival of mycobacteria in host. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of NarL. In turn, NarL binds to the upstream promoter regions of target genes to regulate their expression during aerobic nitrate metabolism. (425 aa) |
| | lpqQ | Rv0835, (MTV043.27), len: 214 aa. Possible lpqQ,lipoprotein. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. (214 aa) |
| | phoR | Rv0758, (MTCY369.03), len: 485 aa. Possible phoR,two component system response phosphate sensor kinase membrane-associated, highly similar to various sensor kinases e.g. CAC32361.1|AL583945 putative two component system histidine kinase from Streptomyces coelicolor (524 aa); NP_349365.1|NC_003030 Membrane-associated sensory histidine kinase with HAMP domain from Clostridium acetobutylicum (482 aa); and similar to phoP proteins e.g. NP_372216.1|NC_002758 alkaline phosphatase synthesis sensor protein from Staphylococcus aureus (554 aa); P23545|PHOR_BACSU alkaline phosphatase synthesis se [...] (485 aa) |
| | PE_PGRS11 | PE-PGRS family protein PE_PGRS11; Induces maturation and activation of human dendritic cells (DCs), via TLR2-dependent activation of ERK1/2, p38 MAPK, and NF-kappa- B signaling pathways, and enhances the ability of DCs to stimulate CD4(+) T cells. By activating DCs, could potentially contribute to the initiation of innate immune responses during tuberculosis infection and hence regulate the clinical course of tuberculosis. Involved in resistance to oxidative stress, via TLR2-dependent activation of the PI3K-ERK1/2-NF-kappa-B signaling pathway and expression of COX-2 and Bcl2. Also abol [...] (584 aa) |
