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| | fadA3 | Rv1074c, (MTV017.27c), len: 405 aa. Probable fadA3,beta-ketoacyl CoA thiolase, highly similar to many involved in beta-oxidation e.g. CAB89028.1|AL353870 beta-ketoadipyl-CoA thiolase from Streptomyces coelicolor (395 aa); P77525|PAAJ_ECOLI probable beta-ketoadipyl CoA thiolase from Escherichia coli (401 aa), FASTA scores: opt: 1034, E(): 5.4e-56, (43.5% identity in 416 aa overlap) and X97452 acetyl-CoA acetyltransferase (thiolase) from Escherichia coli (401 aa), FASTA scores: opt: 1043, E(): 0,(43.4% identity in 415 aa overlap); Q43935|CATF_ACICA beta-ketoadipyl CoA thiolase from Acine [...] (405 aa) |
| | bioF2 | Putative 8-amino-7-oxononanoate synthase 2; Catalyzes the decarboxylative condensation of pimeloyl-[acyl- carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide. (771 aa) |
| | Rv0111 | Rv0111, (MTV031.05), len: 685 aa. Possible transmembrane acyltransferase, equivalent to AA22904.1|AL035300 putative acyltransferase from Mycobacterium leprae (696 aa). Also similar to others e.g. C69975 acyltransferase homolog yrhL from Bacillus subtilis (634 aa), FASTA scores: opt: 520, E(): 4e-22, (36.4% identity in 382 aa overlap). Very similar to Mycobacterium tuberculosis proteins Rv0228, Rv1254, Rv1565c, etc. (685 aa) |
| | ldtA | Probable L,D-transpeptidase LdtA; Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. Is thought to play a role in adaptation to the nonreplicative state of M.tuberculosis. (251 aa) |
| | fbpC | Diacylglycerol acyltransferase/mycolyltransferase Ag85C; The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria to fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha- trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehal [...] (340 aa) |
| | Rv0133 | GCN5-related N-acetyltransferase; Rv0133, (MTCI5.07), len: 201 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Highly similar to others e.g. PUAC_STRLP|P13249 puromycyn N-acetyltransferase (199 aa),FASTA scores: opt: 341, E(): 1.8e-16, (33.3% identity in 201 aa overlap). (201 aa) |
| | Rv0192 | Conserved hypothetical protein; Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. (366 aa) |
| | Rv0221 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (469 aa) |
| | Rv0228 | Rv0228, (MTCY08D5.23), len: 407 aa. Probable integral membrane acyltransferase, equivalent to 3063875|CAA18555.1|AL022486|T44870 acyltransferase from Mycobacterium leprae (384 aa), FASTA scores: opt: 2004,E(): 0, (79.3% identity in 381 aa overlap). Also similar to others e.g. Q11064 probable acyltransferase CY50.28C (383 aa), FASTA scores: opt: 372, E(): 2.6e-16, (35.9% identity in 359 aa overlap); Q00718|MDMB_STRMY acyltransferase. Very similar to Rv0111, Rv1254, etc from Mycobacterium tuberculosis. (407 aa) |
| | htdX | Probable 3-hydroxyacyl-thioester dehydratase HtdX; Shows trans-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydratase activity. Displays a broad chain length specificity, with a predilection for the C8 to C12 substrates. (280 aa) |
| | fadA2 | Rv0243, (MTV034.09), len: 440 aa. Probable fadA2,acetyl-CoA acyltransferase (3-acyl-CoA thiolase),equivalent, but shorter 17 aa, to AL022486|MLCB1883_14T44879 acetyltransferase from Mycobacterium leprae (457 aa), FASTA scores: opt: 250 7,E(): 0, (87.6% identity in 435 aa overlap). Also highly similar to many e.g. G83046|PA478 probable acyl-CoA thiolase from Pseudomonas aeruginosa (425 aa); AB77293.1|AL160312 putative ketoacyl CoA thiolase from Streptomyces coelicolor (428 aa); P76503|7449731|YFCY_ECOLI|D65007|B2342 probable 3-ketoacyl-CoA thiolase (acetyl-CoA acyltransferase) (beta-ket [...] (440 aa) |
| | aac | Aminoglycoside 2'-N-acetyltransferase Aac (Aac(2')-IC); May catalyze the coenzyme A-dependent acetylation of the 2' hydroxyl or amino group of a broad spectrum of aminoglycosides and confer resistance to aminoglycosides (By similarity). In vitro assays show no significant increase of resistance to aminoglycosides, possibly due to low expression in a heterologous system. Belongs to the AAC(2')-I acetyltransferase family. (181 aa) |
| | pks6 | Rv0405, (MTCY22G10.01), len: 1402 aa. Probable pks6,membrane-bound polyketide synthase (see citation below),highly similar to others e.g. CAC29643.1|AL583917 putative polyketide synthase from Mycobacterium leprae (2103 aa); Y06K_MYCTU|Q10977 probable polyketide synthase (1876 aa),FASTA scores: opt: 2303, E(): 0, (38.7% identity in 1232 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site, 2 x PS00017 ATP/GTP-binding site motif A (P-loop), and PS00012 Phosphopantetheine attachment site. (1402 aa) |
| | pta | Probable phosphate acetyltransferase Pta (phosphotransacetylase); Involved in acetate metabolism; In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family. (690 aa) |
| | lprQ | Probable conserved lipoprotein LprQ; Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. (451 aa) |
| | Rv0502 | Conserved protein; Rv0502, (MTCY20G9.29), len: 358 aa. Conserved protein, equivalent to P54878|Y502_MYCLE|ML2427 hypothetical 40.5 KDA protein from Mycobacterium leprae (367 aa), FASTA scores: opt: 2042, E(): 0, (84.1% identity in 365 aa overlap). Also similar to T36273|SCE68.23c hypothetical protein from Streptomyces coelicolor (355 aa). C-terminal similar to AL021529|SC10A5_4|T34572 hypothetical protein from Streptomyces coelicolor (295 aa), FASTA score: (57.8% identity in 263 aa overlap); and to hypothetical proteins from Mycobacterium tuberculosis Rv1920|G70808 (287 aa); and Rv1428 [...] (358 aa) |
| | Rv0517 | Rv0517, (MTCY20G10.07), len: 436 aa. Possible acyltransferase, integral membrane protein, equivalent (but longer 26 aa in N-terminus) to AAK44761.1|AE006954 putative acyltransferase from Mycobacterium tuberculosis strain CDC1551 (410 aa). Also similar to many acyltransferases e.g. MDMB_STRMY|Q00718 from Streptomyces mycarofaciens (387 aa), FASTA scores: opt: 200, E(): 1.1e-08, (28.2% identity in 394 aa overlap). And similar to Rv0111, Rv0228, Rv1254,Rv1565c from Mycobacterium tuberculosis. (436 aa) |
| | Rv0519c | Rv0519c, (MTCY20G10.09c), len: 300 aa. Possible conserved membrane protein, with hydrophobic region near N-terminus. Could be a lipase. Similar to Rv0774c|MTCY369.19c|A70708 from Mycobacterium tuberculosis (312 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00120 Lipases, serine active site. (300 aa) |
| | fabH | 3-oxoacyl-[acyl-carrier-protein] synthase III FabH (beta-ketoacyl-ACP synthase III) (KAS III); Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Has some substrate specificity for long chain acyl-CoA such as myristoyl-CoA. Does not use acyl-CoA as primer. Its substrate spec [...] (335 aa) |
| | fabD2 | Rv0649, (MTCY20H10.30), len: 224 aa. Possible fabD2,malonyl CoA-acyl carrier protein transacylase, similar to mtfabd|FABD_MYCTU|Q10501|Rv2243 malonyl CoA-acyl carrier protein transacylase from Mycobacterium tuberculosis (302 aa), FASTA scores: opt: 133, E(): 0.074, (31.3% identity in 147 aa overlap). (224 aa) |
| | Rv0730 | GCN5-related N-acetyltransferase; Rv0730, (MTV041.04), len: 242 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to Z98756|MLCB2492_26 hypothetical protein from Mycobacterium leprae (227 aa), FASTA scores: opt: 1180, E(): 0, (83.5% identity in 218 aa overlap). (242 aa) |
| | ggtA | Gamma-glutamyltranspeptidase / glutathione hydrolase; Rv0773c, (MTCY369.18), len: 512 aa. Probable ggtA,bifunctional acylase including cephalosporin acylase, and gamma-glutamyl transpeptidase; highly similar to others e.g. NP_295247.1|NC_001263 cephalosporin acylase from Deinococcus radiodurans (535 aa); NP_248854.1|NC_002516 probable gamma-glutamyltranspeptidase from Pseudomonas aeruginosa (538 aa); P15557|PAC1_PSES3 acylase ACY 1 [includes: cephalosporin acylase (GL-7ACA acylase); gamma-glutamyltranspeptidase (GGT)] from Pseudomonas sp. strain SE83 (558 aa), FASTA scores: opt: 784, E [...] (512 aa) |
| | Rv0774c | Rv0774c, (MTCY369.19c), len: 303 aa. Possible conserved exported protein with hydrophobic region near N-terminus, highly similar, except in N-terminus, to Rv0519c|Z97831|MTY20G10.09c|O33364 hypothetical protein from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature, and PS00120 Lipases, serine active site. So could be a lipase. Start changed since first submission (-9 aa). Predicted to be an outer membrane protein (See Song et al., 2008). (303 aa) |
| | Rv0802c | Possible succinyltransferase in the GCN5-related N-acetyltransferase family; May function as a succinyl-CoA transferase. (218 aa) |
| | mshD | GCN5-related N-acetyltransferase, MshD; Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol. Belongs to the acetyltransferase family. MshD subfamily. (315 aa) |
| | fadA | Rv0859, (MTV043.52), len: 403 aa. Possible fadA,acyl-CoA thiolase, equivalent to NP_302423.1|NC_002677 putative beta-ketoadipyl CoA thiolase from Mycobacterium leprae (403 aa). Also highly similar to acyl/acetyl-CoA thiolases and beta-ketoadipyl CoA thiolases, e.g. T35428 probable acetyl CoA acetyltransferase (thiolase) from Streptomyces coelicolor (404 aa); NP_250427.1|NC_002516 probable acyl-CoA thiolase from Pseudomonas aeruginosa (401 aa); NP_106253.1|NC_002678 probable acyl-CoA thiolase from Mesorhizobium loti (402 aa); NP_248919.1|NC_002516|PcaF beta-ketoadipyl CoA thiolase PcaF [...] (403 aa) |
| | citA | Probable citrate synthase II CitA; Rv0889c, (MTCY31.17c), len: 373 aa. Probable citA (alternate gene name: gltA), citrate synthase 2, highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30,(33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. (373 aa) |
| | Rv0895 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (505 aa) |
| | gltA2 | Rv0896, (MTCY31.24), len: 431 aa. Probable gltA2,citrate synthase 1, highly similar to O33066|NP_302405.1|NC_002677 citrate synthase 1 from Mycobacterium leprae (431 aa), FASTA scores: E(): 0, (91.0 identity in 431 aa overlap); and AAF04133.1|AF191033_1|AF191033 citrate synthase from Mycobacterium smegmatis (441 aa). Also highly similar to others e.g. AAF14286.1|AF181118_1|AF181118 citrate synthase from Streptomyces coelicolor (429 aa); P42457|CISY_CORGL citrate synthase from Corynebacterium glutamicum (437 aa),FASTA scores: opt: 1847, E(): 0, (63.0% identity in 433 aa overlap); etc. A [...] (431 aa) |
| | Rv0914c | Rv0914c, (MTCY21C12.08c), len: 412 aa. Possible lipid carrier protein or keto acyl-CoA thiolase, highly similar to NP_421905.1|NC_002696 thiolase family protein from Caulobacter crescentus (407 aa); and similar to others e.g. NP_107896.1|NC_002678 3-ketoacyl-CoA thiolase from Mesorhizobium loti (392 aa); NP_385796.1|NC_003047 putative 3-ketoacyl-CoA thiolase protein from Sinorhizobium meliloti (389 aa); NP_275932.1|NC_000916 lipid-transfer protein (sterol or nonspecific) from Methanothermobacter thermautotrophicus (383 aa); AB55378.1|AL117263 possible 3-ketoacyl-CoA thiolase from Leish [...] (412 aa) |
| | Rv0919 | GCN5-related N-acetyltransferase; Rv0919, (MTCY21C12.13), len: 166 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Some similarity to Q50115 hypothetical protein from Mycobacterium leprae (90 aa), FASTA scores: opt: 243, E(): 5.3e-11, (56.5% identity in 85 aa overlap). Alternative nucleotide at position 1025106 (T->C; F141F) has been observed. (166 aa) |
| | rimJ | [ribosomal protein S5]-alanine N-acetyltransferase; Rv0995, (MTCI237.09), len: 203 aa. RimJ,ribosomal-protein-alanine acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to AL035500|MLCL373_24 probable acyltransferase from Mycobacterium leprae (218 aa), FASTA scores: (86.0% identity in 200 aa overlap). Also similar to others and many acyltransferases e.g. BAB69252.1|AB070946 possible acyltransferase from Streptomyces avermitilis (156 aa); NP_385025.1|NC_003047 probable ribosomal-protein-alanine acetyltransferase from Sinorhizo [...] (203 aa) |
| | Rv0998 | Conserved hypothetical protein; Catalyzes specifically the acetylation of the epsilon-amino group of a highly conserved lysine residue in acetyl-CoA synthetase (ACS). This acetylation results in the inactivation of ACS activity and could be important for mycobacteria to adjust to environmental changes. (333 aa) |
| | glmU | Probable UDP-N-acetylglucosamine pyrophosphorylase GlmU; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain. (495 aa) |
| | Rv1125 | Rv1125, (MTCY22G8.14), len: 414 aa. Conserved hypothetical protein. Similar to AL133278|SCM11.13 hypothetical protein from Streptomyces coelicolor (446 aa),FASTA scores: opt: 182, E(): 0.0005, (28.1% identity in 437 aa overlap). (414 aa) |
| | prpC | Probable methylcitrate synthase PrpC; Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the Claisen condensation of propionyl-CoA and oxaloacetate (OAA) to yield 2-methylcitrate (2-MC) and CoA. Also catalyzes the condensation of oxaloacetate with acetyl-CoA. (393 aa) |
| | Rv1135A | Rv1135A, len: 80 aa. Possible acetyl-CoA acetyltransferase (possible gene fragment), highly similar to other acetyl-CoA acetyltransferases e.g. C-terminal part of Rv3556c|Z92774|MTCY6G11_2|MTCY06G11.03|fadA6 acetyl-CoA acetyltransferase from Mycobacterium tuberculosis (386 aa),FASTA scores: opt: 219, E(): 5.7e-09, (63.6% identity in 55 aa overlap). (80 aa) |
| | pks3 | Rv1180, (MTV005.16), len: 488 aa. Probable polyketide beta-ketoacyl synthase, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and similar to the N-terminus of many polyketide synthases e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 2115, E(): 0, (66.5% identity in 472 aa overlap). Also similar to, and same length as P96284|Z83858|MTCY24G1.02 M. tuberculosis (496 aa), FASTA scores: opt: 1424, E(): 0, (50.9% identity in 444 aa overlap). Contains possible signal sequence and PS00013 Pr [...] (488 aa) |
| | pks4 | Probable polyketide beta-ketoacyl synthase Pks4; Polyketide synthase involved in the biosynthesis of methyl- branched fatty acids such as mycolipanoic, mycolipenic (phthienoic) and mycolipodienoic acids required for the synthesis of a major class of polyacylated trehaloses. Catalyzes the elongation of CoA esters of long-chain fatty acids by incorporation of three methylmalonyl (but not malonyl) residues, to form trimethyl-branched fatty-acids. (1582 aa) |
| | papA3 | Probable conserved polyketide synthase associated protein PapA3; Involved in the biosynthesis of polyacyltrehalose (PAT) which could have a role in anchoring the bacterial capsule. In vitro catalyzes the sequential transfer of two palmitoyl groups onto a single glucose residue of trehalose generating the diacylated product 2,3- diacyltrehalose (trehalose dipalmitate). Although palmitoyl-CoA (PCoA) seems to be the physiological acyl donor, PapA3 can also use docosanoyl (22-carbon saturated fatty acid) coenzyme A as acyl donor. (472 aa) |
| | chp2 | Possible exported protein; Involved in the final steps of polyacyltrehalose (PAT) biosynthesis. Catalyzes the transfer of three mycolipenoyl groups onto diacyltrehalose (DAT) to form PAT. (359 aa) |
| | dapD | Tetrahydrodipicolinate N-succinyltransferase DapD; Catalyzes the conversion of the cyclic tetrahydrodipicolinate (THDP) into the acyclic N-succinyl-L-2-amino-6-oxopimelate using succinyl-CoA. (317 aa) |
| | kgd | Multifunctional alpha-ketoglutarate metabolic enzyme; Shows three enzymatic activities that share a first common step, the attack of thiamine-PP on 2-oxoglutarate (alpha-ketoglutarate, KG), leading to the formation of an enamine-thiamine-PP intermediate upon decarboxylation. Thus, displays KGD activity, catalyzing the decarboxylation from five-carbon 2-oxoglutarate to four-carbon succinate semialdehyde (SSA). Also catalyzes C-C bond formation between the activated aldehyde formed after decarboxylation of alpha- ketoglutarate and the carbonyl of glyoxylate (GLX), to yield 2-hydroxy- 3-o [...] (1231 aa) |
| | Rv1254 | Rv1254, (MTCY50.28c), len: 383 aa. Probable Acyltransferase, similar to G927228 midecamycin 4-0-propionyl transferase (fragment) (388 aa), FASTA scores, opt: 305, E(): 5.6e-14, (28.4% identity in 377 aa overlap). Also similar to other Mycobacterium tuberculosis acyltransferases e.g. Rv0111, Rv0228, etc. Contains PS00881 Protein splicing signature. (383 aa) |
| | Rv1288 | Conserved protein; Exhibits lipolytic activity with medium chain length esters as optimum substrates. In vitro, pNP-caprylate (C8) is the optimum substrate followed by pNP-capricate (C10). May modulate the cell wall lipids to favor the survival of bacteria under stress conditions. (456 aa) |
| | fadA4 | Rv1323, (MTCY130.08), len: 389 aa. Probable fadA4,acetyl-CoA acetyltransferase, equivalent to THIL_MYCLE|P46707 possible acetyl-CoA C-acetyltransferase from Mycobacterium leprae (393 aa), FASTA scores: opt: 2218, E(): 0, (87.0% identity in 392 aa overlap). Also highly similar to others e.g. CAB70629.1|AL137242 probable acetoacetyl-CoA thiolase from Streptomyces coelicolor (401 aa); T51772 acetyl-CoA C-acetyltransferase [validated] from Alcaligenes latus (392 aa); etc. Some homologies indicate ATA start codon. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiola [...] (389 aa) |
| | mbtK | Lysine N-acetyltransferase MbtK; Acyltransferase required for the direct transfer of medium- to long-chain fatty acyl moieties from a carrier protein (MbtL) on to the epsilon-amino group of lysine residue in the mycobactin core. (210 aa) |
| | pks18 | Conserved hypothetical protein; Involved in the biosynthesis of tri- and tetraketide alpha- pyrones. Pks18 catalyzes the extension of medium- and long-chain aliphatic acyl-CoA substrates by using malonyl-CoA as an extender molecule to synthesize polyketide products. Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family. (393 aa) |
| | Rv1425 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (459 aa) |
| | Rv1428c | Rv1428c, (MTCY493.26), len: 275 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0502|YV29_MYCTU|Q11167 (358 aa), FASTA scores: opt: 355,E(): 5e-16, (32.6% identity in 273 aa overlap); and Rv1920. (275 aa) |
| | Rv1433 | Possible conserved exported protein; Probable L,D-transpeptidase that may perform as-yet-unknown cross-linking reactions in M.tuberculosis. Is not able to generate 3->3 cross-links in peptidoglycan, using tetrapeptide stems as acyl donor substrates. May function in the anchoring of proteins to peptidoglycan. (271 aa) |
| | fabG1 | 3-oxoacyl-[acyl-carrier-protein] reductase FabG1; Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis. MabA preferentially metabolizes long-chain substrates (C8-C20) and has a poor affinity for the C4 substrate; Belongs to the short-chain dehydrogenases/reductases (SDR) family. (247 aa) |
| | inhA | NADH-dependent enoyl-[acyl-carrier-protein] reductase InhA (NADH-dependent enoyl-ACP reductase); Enoyl-ACP reductase of the type II fatty acid syntase (FAS- II) system, which is involved in the biosynthesis of mycolic acids, a major component of mycobacterial cell walls. Catalyzes the NADH-dependent reduction of the double bond of 2-trans- enoyl-[acyl-carrier protein], an essential step in the fatty acid elongation cycle of the FAS-II pathway. Shows preference for long-chain fatty acyl thioester substrates (>C16), and can also use 2-trans-enoyl-CoAs as alternative substrates. The mycob [...] (269 aa) |
| | pks5 | Probable polyketide synthase Pks5; Polyketide synthase likely involved in the biosynthesis of a polymethyl-branched fatty acid (PMB-FA) that might only be produced during host infection. Is required for the full virulence of M.tuberculosis during host infection. (2108 aa) |
| | Rv1533 | Conserved protein; Rv1533, (MTCY07A7A.02), len: 375 aa. Conserved protein. Similar to 2NPD_NEUCR|Q01284 2-nitropropane dioxygenase precursor (378 aa), fasta scores: opt: 279,E(): 9.1e-11, (31.3% identity in 256 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1894c, Rv0021c, Rv3553, Rv2781c. (375 aa) |
| | plsB1 | Rv1551, (MT1601, MTCY48.14c), len: 621 aa. Possible plsB1, acyltransferase, similar to PLSB_HAEIN|P44857 glycerol-3-phosphate acyltransferase from Haemophilus influenzae (810 aa), FASTA scores: opt: 434, E(): 6.2e-22,(27.6% identity in 395 aa overlap). Also similar to Rv2482c|plsB2 Probable glycerol-3-phosphate acyltransferase from Mycobacterium tuberculosis (789 aa). (621 aa) |
| | Rv1565c | Rv1565c, (MTCY336.38), len: 729 aa. Conserved hypothetical membrane protein, some similarity to O05402 hypothetical 72.2 kDa protein from Bacillus subtilis (634 aa), FASTA results: opt: 384, E(): 4.8e-17, (29.1% identity in 378 aa overlap); and to Y392_HAEIN|P43993 hypothetical protein hi0392 from H. influenzae (245 aa), FASTA results: opt: 265, E(): 5.5e-10, (28.3% identity in 247 aa overlap). C-terminal half equivalent to AL049478|MLCL458_19 (274 aa) (78.5% identity in 274 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0111,Rv0228, Rv1254, Rv0517. N-t [...] (729 aa) |
| | bioF1 | 8-amino-7-oxononanoate synthase 1; Catalyzes the decarboxylative condensation of pimeloyl-[acyl- carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide (By similarity). Can also use pimeloyl-CoA instead of pimeloyl-ACP as substrate. To a lesser extent, can also utilize D-alanine instead of L-alanine as substrate. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily. (386 aa) |
| | Rv1619 | Conserved membrane protein; Rv1619, (MTCY01B2.11), len: 484 aa. Conserved membrane protein. Some similarity to N-terminus of P94974|Rv1640c|MTCY06H11.04c probable lysyl-tRNA synthetase 2 from Mycobacterium tuberculosis (1172 aa), FASTA scores: E(): 1.4e-16, (28.0% identity in 410 aa overlap); and similar in part to O69916| SC3C8.03C Putative intergral membrane protein from Streptomyces coelicolor cosmid 3C8 (589 aa), FASTA scores: opt: 453 E(): 8.4e-22, (31.3% identity in 313 aa overlap). (484 aa) |
| | Rv1627c | Rv1627c, (MTCY01B2.19c), len: 402 aa. Probable nonspecific lipid-transfer protein, similar to many lipid carrier proteins e.g. Q51797 acetyl CoA synthase from Pyrococcus furiosus (388 aa), FASTA scores: opt: 400, E(): 3.2e-18, (34.4% identity in 407 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis proteins Rv3523,Rv3540c, Rv0244, Rv2790c, Rv1323, etc. (402 aa) |
| | lysX | Lysyl-tRNA synthetase 2 LysX; Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lys [...] (1172 aa) |
| | argJ | Probable glutamate N-acetyltransferase ArgJ; Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate. (404 aa) |
| | pks10 | Chalcone synthase Pks10; Could catalyze the elongation of hydroxybenzoyl-CoA as well as elongation of the aliphatic precursor involved in the synthesis of phthiocerol dimycocerosate (DIM); Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family. (353 aa) |
| | pks7 | Rv1661, (MTCY06H11.26), len: 2126 aa. Probable pks7,polyketide synthase, similar to many e.g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 (3567 aa), FASTA scores: E(): 0, (48.8% identity in 2131 aa overlap); also similar to Mycobacterium tuberculosis pks12. Contains PS00606 Beta-ketoacyl synthases active site, PS00012 Phosphopantetheine attachment site. (2126 aa) |
| | pks8 | Rv1662, (MTCY275.01-MTCY06H11.27), len: 1602 aa. Probable pks8, polyketide synthase, similar to many polyketide synthases e.g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 from Saccharopolyspora erythraea (Streptomyces erythraeus) (3567 aa), FASTA scores: opt: 3319, E(): 0, (45.8% identity in 1619 aa overlap). Also similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks7 and pks12. Contains PS00606 Beta-ketoacyl synthases active site and PS01162 Quinone oxidoreductase/zeta-crystallin signature. Note that the similarity extends into the downstream [...] (1602 aa) |
| | pks17 | Rv1663, (MTCY275.02), len: 502 aa. Probable pks17,polyketide synthase, similar to other polyketide synthases e g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 (3567 aa) from Saccharopolyspora erythraea (Streptomyces erythraeus), FASTA scores: opt: 1207, E(): 0,(43.9% identity in 531 aa overlap). Also similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks7 and pks1. Note that the similarity extends into the upstream ORF Rv1662 (MTCY275.01) and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy w [...] (502 aa) |
| | pks9 | Rv1664, (MTCY275.03), len: 1017 aa. Probable pks9,polyketide synthase, similar to OL56_STRAT|Q07017 oleandomycin polyketide synthase, modules 5 and 6 from Streptomyces antibioticus (3519 aa), FASTA scores: opt: 1767, E(): 0, (41.6% identity in 919 aa overlap). Similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks6, pks8, etc. Contains PS00012 Phosphopantetheine attachment site. (1017 aa) |
| | pks11 | Chalcone synthase Pks11; Involved in the biosynthesis of tri- and tetraketide alpha- pyrones. Pks11 catalyzes the extension of medium- and long-chain aliphatic acyl-CoA substrates by using malonyl-CoA as an extender molecule to synthesize polyketide products. (353 aa) |
| | Rv1734c | Rv1734c, (MTCY04C12.19c), len: 80 aa. Conserved hypothetical protein, similar to C-terminal region Q9Z8N2|CP0452|AE001615 Dihydrolipoamide Acetyltransferase from Chlamydia pneumoniae (429 aa), FASTA scores: opt: 138,E(): 0.0012, (26.9% identity in 78 aa overlap). (80 aa) |
| | Rv1760 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (502 aa) |
| | glcB | Malate synthase G GlcB; Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl- CoA) and glyoxylate to form malate and CoA. (741 aa) |
| | Rv1867 | Conserved protein; Rv1867, (MTCY359.06c), len: 494 aa. Conserved protein, some similarity to acetyl CoA synthase and to lipid carriers. FASTA best: E155295 acetyl CoA synthase (388 aa), opt: 213, E(): 4.5e-07, (23.2% identity in 423 aa overlap). (494 aa) |
| | fbpB | Diacylglycerol acyltransferase/mycolyltransferase Ag85B; The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha- trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-treha [...] (325 aa) |
| | Rv1894c | Rv1894c, (MTCY180.24), len: 376 aa. Conserved hypothetical protein, weak similarity to some oxidoreductases e.g. Q01284 2-nitropropane dioxygenase precursor (378 aa), FASTA results: opt: 204, E(): 5.8e-06,(34.3% identity in 140 aa overlap). Similar to hypothetical Mycobacterium tuberculosis proteins e.g. Rv3553|MTCY03C7.02c (355 aa), FASTA results: opt: 296, E(): 1.6e-10, (32.9% identity in 167 aa overlap); Rv1533 (375 aa) (48.1% identity in 376 aa overlap); Rv0021c, Rv2781c. (376 aa) |
| | Rv1920 | Probable membrane protein; Rv1920, (MTV050.04), len: 287 aa. Probable membrane protein, similar to AL0215|SC10A5.04 putative membrane protein from Streptomyces coelicolor cosmid 10A5 (295 aa),FASTA scores: opt: 292, E(): 3.6e-13, (31.3% identity in 243 aa overlap). Also weakly similar to several Mycobacterial putative proteins with unknown function e.g. Rv0502, Rv1428c, U00018_22 Mycobacterium leprae cosmid B2168. (287 aa) |
| | pks12 | Polyketide synthase Pks12; Rv2048c, (MTV018.35c), len: 4151 aa. Pks12,polyketide synthase similar to many. Contains 2x PS00012 Phosphopantetheine attachment site, 2x PS00606 Beta-ketoacyl synthases active site, and PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. Nucleotide position 2297976 in the genome sequence has been corrected, G:A resulting in S3004L. (4151 aa) |
| | ppm1 | Polyprenol-monophosphomannose synthase Ppm1; Catalyzes the phospholipid dependent N-acylation of the N- terminal cysteine of apolipoprotein, the last step in lipoprotein maturation; In the N-terminal section; belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily. (874 aa) |
| | Rv2170 | GCN5-related N-acetyltransferase; Rv2170, (MTV021.03), len: 206 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to hypothetical protein ML0903 (210 aa) from Mycobacterium leprae. FASTA scores: ML0903 conserved hypothetical protein (210 aa) opt: 1045, E(): 9.1e-57; 77.143% identity in 210 aa overlap. >emb|CAA18679.1| (AL022602) >gi|13092973|emb|CAC31284.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (206 aa) |
| | Rv2182c | Rv2182c, (MTV021.15c), len: 247 aa. Probable 1-acylglycerol-3-phosphate O-acyltransferase, similar to many e.g. in Streptomyces. Contains PS00017 ATP/GTP-binding site motif A (P-loop). FASTA scores: pir||T35503 1-acylglycerol-3-phosphate O-acyltransferase homolog SC6E10.16c - Streptomyces coelicolor >gi|5689932|emb|CAB51970.1| (AL109661) hypothetical protein [Streptomyces coelicolor A3(2)] Length = 262, Expect = 6e-61 (54% identity in 215 aa overlap). (247 aa) |
| | dlaT | DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase; Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). Appears to be essential for Mtb pathogenesis. (553 aa) |
| | lipB | Probable lipoate biosynthesis protein B LipB; Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate- dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate. Belongs to the LipB family. (230 aa) |
| | fabD | Rv2243, (MTCY427.24), len: 302 aa. FabD (alternate gene name: mtFabD), malonyl CoA-acyl carrier protein transacylase (see citations below), highly similar to e.g. A57356 acyl-CoA carrier protein malonyltransferase from Streptomyces coelicolor (316 aa), FASTA score: opt: 955,E(): 0, (52.6% identity in 304 aa overlap); FABD_HAEIN|P43712 malonyl CoA-acyl carrier protein transacylase from Haemophilus influenzae, FASTA score: (30.5% identity in 308 aa overlap); and FABD_ECOLI|P25715 from Escherichia coli, FASTA score: (31.4% identity in 309 aa overlap). Identified as a substrate for proteas [...] (302 aa) |
| | kasA | 3-oxoacyl-[acyl-carrier protein] synthase 1 KasA (beta-ketoacyl-ACP synthase) (KAS I); Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family. (416 aa) |
| | kasB | 3-oxoacyl-[acyl-carrier protein] synthase 2 KasB (beta-ketoacyl-ACP synthase) (KAS I); Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. (438 aa) |
| | Rv2262c | Conserved hypothetical protein; Catalyzes the phospholipid dependent N-acylation of the N- terminal cysteine of apolipoprotein, the last step in lipoprotein maturation. (360 aa) |
| | Rv2275 | Conserved hypothetical protein; Involved in the biosynthesis of mycocyclosin. It uses activated amino acids in the form of aminoacyl-tRNAs (aa-tRNAs) as substrates to catalyze the ATP-independent formation of cyclodipeptides which are intermediates in diketopiperazine (DKP) biosynthetic pathways. Catalyzes the formation of cyclo(L-Tyr-L-Tyr) (cYY) from L- tyrosyl-tRNA(Tyr). Can incorporate various nonpolar residues, such as L-phenylalanine, L-leucine, L-tyrosine and L-methionine, and to a much lesser extent L-alanine, into cyclodipeptides. Cyclodipeptides synthesized by Rv2275 always c [...] (289 aa) |
| | Rv2285 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (445 aa) |
| | cysE | Probable serine acetyltransferase CysE (sat); Catalyzes the acetylation of serine by acetyl-CoA to produce O-acetylserine (OAS); Belongs to the transferase hexapeptide repeat family. (229 aa) |
| | mbtC | Polyketide synthetase MbtC (polyketide synthase); Rv2382c, (MTCY22H8.03), len: 444 aa. MbtC,polyketide synthase (see citations below), similar in part to several synthases e.g. Q9F7T9 avermectin polyketide synthase (fragment) from Streptomyces avermitilis (3626 aa), FASTA scores: opt: 1458, E(): 7e-82, (50.65% identity in 446 aa overlap); AAG23264|SPNA polyketide synthase loading and extender module 1 from Saccharopolyspora spinosa (2595 aa) FASTA scores: opt: 1441, E(): 6e-81,(49.1% identity in 446 aa overlap); O33954|TYLG tylactone synthase starter module and modules 1 & 2 from Strep [...] (444 aa) |
| | eis | Enhanced intracellular survival protein Eis,GCN5-related N-acetyltransferase; Effector that is released into the host cell and affects host immune responses; it negatively modulates inflammation, macrophage autophagy, and cell death through redox-dependent signaling. Acts as an acetyltransferase. Acetylates 'Lys-55' of dual-specificity protein phosphatase 16 (DUSP16)/mitogen-activated protein kinase phosphatase-7 (MKP-7), a JNK- specific phosphatase; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced [...] (402 aa) |
| | octT | Unknown protein; Sugar octanoyltransferase likely involved in the biosynthesis of mycobacterial methylglucose lipopolysaccharide (MGLP). Catalyzes the transfer of an octanoyl group from octanoyl-CoA to the C6 OH of the second glucose in diglucosylglycerate (DGG). DGG is the preferred acceptor, but to a lesser extent, GG (glucosylglycerate) can also be used as substrate. DGG and GG are the two earliest intermediates in MGLP biosynthesis. (247 aa) |
| | plsB2 | Rv2482c, (MT2555, MTV008.38c), len: 789 aa. Probable plsB2, glycerol-3-phosphate acyltransferase, highly similar to Q9X7B0|PLSB_MYCLE probable glycerol-3-phosphate acyltransferase from Mycobacterium leprae (775 aa), FASTA scores: opt: 4210, E(): 0, (80.7% identity in 783 aa overlap). Also similar to others e.g. P00482|PLSB_ECOLI from Escherichia coli (806 aa), FASTA scores: opt: 521,E(): 3e-24, (24.35 identity in 612 aa overlap); Q9CLN7|PLSB_PASMU from Pasteurella multocida (809 aa),FASTA scores: opt: 529, E(): 9.7e-25, (27.05% identity in 540 aa overlap); Q9KVP8|PLSB_VIBCH from Vibrio [...] (789 aa) |
| | plsC | PlsC domain-containing protein; Rv2483c, (MTV008.39c), len: 580 aa. Possible plsC, a transmembrane phospholipid biosynthesis bifunctional enzyme, including L-3-phosphoserine phosphatase and 1-acyl-Sn-glycerol-3-phosphate acyltransferase, equivalent to Q9X7A9|PLSC|ML1245 putative acyltransferase from Mycobacterium leprae (579 aa), FASTA scores: opt: 2835,E(): 9.2e-153, (77.15% identity in 573 aa overlap). C-terminal end is similar to many 1-acyl-SN-glycerol-3-phosphate acyltransferases (lysophosphatidic acidacyltransferases) e.g. Q9SDQ2 from Limnanthes floccosa (281 aa), FASTA scores: o [...] (580 aa) |
| | Rv2484c | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity; Belongs to the long-chain O-acyltransferase family. (491 aa) |
| | bkdC | Probable branched-chain keto acid dehydrogenase E2 component BkdC; Component of the branched-chain alpha-ketoacid dehydrogenase (BCKADH) complex, that catalyzes the overall conversion of branched- chain alpha-ketoacids to acyl-CoA and CO(2). (393 aa) |
| | ldtB | Probable L,D-transpeptidase LdtB; Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. (408 aa) |
| | fas | Rv2524c, (MTCY159.32, MTV009.09c), len: 3069 aa. Probable fas, Fatty Acid Synthase, equivalent to Q9X7E2|fas|ML1191 putative type I fatty acid synthase from Mycobacterium leprae (3076 aa), FASTA scores: opt: 17484,E(): 0, (85.8% identity in 3081 aa overlap). Also similar to others e.g. Q04846|fas|Q59497 from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (3104 aa), FASTA scores: opt: 3981, E(): 5.5e-203, (49.8% identity in 3099 aa overlap); Q48926|fas from Mycobacterium bovis (2796 aa),FASTA scores: opt: 2098, E(): 3.9e-103, (59.7% identity in 2862 aa overlap) (see Fernande [...] (3069 aa) |
| | Rv2529 | Hypothetical protein; Rv2529, (MTCY159.27c), len: 463 aa. Hypothetical unknown protein. Note that C-terminal part is similar to short region of Q53609|MTS1_STRAL|SALIM modification methylase SALI from Streptomyces albus G (587 aa), FASTA scores: opt: 170, E(): 0.016, (59.45% identity in 37 aa overlap). (463 aa) |
| | Rv2611c | Probable acyltransferase; Catalyzes the acylation to the position 6 of the alpha-1,2- linked mannose residue of the phosphatidyl-myo-inositol dimannoside (PIM2) or monomannoside (PIM1). (316 aa) |
| | Rv2669 | GCN5-related N-acetyltransferase; Rv2669, (MTCY441.38), len: 156 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Similarity to several proteins e.g. Q9A6M0|CC2073 acetyltransferase (GNAT family) from Caulobacter crescentus (178 aa), FASTA scores: opt: 242, E(): 1.2e-09, (30.9% identity in 165 aa overlap); Q99RQ8|SA2159 hypothetical protein similar to transcription repressor of sporulation,septation and degradation paiA from Staphylococcus aureus subsp. aureus N315 (171 aa), FASTA scores: opt: 214, E(): 9.8e-08, (27.5% id [...] (156 aa) |
| | argA | Probable L-glutamate alpha-N-acetyltranferase ArgA (alpha-N-acetylglutamate synthase); Catalyzes the conversion of L-glutamate to alpha-N-acetyl-L- glutamate. L-glutamine is a significantly better substrate compared to L-glutamate; Belongs to the acetyltransferase family. (174 aa) |
| | Rv2775 | GCN5-related N-acetyltransferase; Rv2775, (MTV002.40), len: 153 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Showing weak similarity to other hypothetical proteins e.g. Q9ZBJ7|SC9C7.13c from Streptomyces coelicolor (179 aa),FASTA scores: opt: 167, E(): 0.00024, (29.05% identity in 148 aa overlap). Equivalent to AAK47164 from Mycobacterium tuberculosis strain CDC1551 (185 aa) but shorter 32 aa. (153 aa) |
| | ltp1 | Rv2790c, (MTV002.55c), len: 401 aa. Probable ltp1,lipid-transfer protein, highly similar to many eukaryotic sterol-carrier proteins/lipid-transfer protein precursors (see Ossendorp & Wirtz 1993) e.g. O62742|SCP2 sterol carrier protein X from Oryctolagus cuniculus (Rabbit) (547 aa), FASTA scores: opt: 1710, E(): 6e-102, (63.7% identity in 394 aa overlap); Q9QW19 3-oxoacyl-CoA thiolase homolog (fragment) from Rattus sp. (405 aa), FASTA scores: opt: 1696, E(): 3.8e-101, (63.2% identity in 394 aa overlap); P11915|NLTP_RAT|SCP2|SCP-2 nonspecific lipid-transfer protein precursor from Rattus [...] (401 aa) |
| | Rv2851c | GCN5-related N-acetyltransferase; Rv2851c, (MTCY24A1.06), len: 156 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Similar to others e.g. Q9KP14|VC2565 ELAA protein from Vibrio cholerae (149 aa), FASTA scores: opt: 360, E(): 1e-18, (46.05% identity in 139 aa overlap); Q9I717|PA0115 hypothetical protein from Pseudomonas aeruginosa (150 aa),FASTA scores: opt: 341, E(): 2.4e-17, (43.65% identity in 142 aa overlap); Q9K8M4|BH2982 hypothetical protein from Bacillus halodurans (155 aa), FASTA scores: opt: 320, E(): 8e-16, (40. [...] (156 aa) |
| | Rv2867c | GCN5-related N-acetyltransferase; Rv2867c, (MTV003.13c), len: 284 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Similar to others e.g. Q9KYR8|SC5H4.21 hypothetical 31.3 KDA protein from Streptomyces coelicolor (287 aa), FASTA scores: opt: 798, E(): 2.4e-45, (47.95% identity in 269 aa overlap). (284 aa) |
| | ppsA | Phenolpthiocerol synthesis type-I polyketide synthase PpsA; Involved in the elongation of either C22-24 fatty acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol derivatives. (1876 aa) |
| | ppsB | Phenolpthiocerol synthesis type-I polyketide synthase PpsB; Involved in the elongation of either C22-24 fatty acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol derivatives. (1538 aa) |
| | ppsC | Phenolpthiocerol synthesis type-I polyketide synthase PpsC; Involved in the elongation of either C22-24 fatty acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol derivatives. (2188 aa) |
| | ppsD | Phenolpthiocerol synthesis type-I polyketide synthase PpsD; Involved in the elongation of either C22-24 fatty acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol derivatives. (1827 aa) |
| | ppsE | Phenolpthiocerol synthesis type-I polyketide synthase PpsE; Involved in the elongation of either C22-24 fatty acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol derivatives. (1488 aa) |
| | papA5 | Possible conserved polyketide synthase associated protein PapA5; Catalyzes diesterification of phthiocerol, phthiodiolone, and phenolphthiocerol with mycocerosic acids, the final step in the phthiocerol, phthiodiolone and phenolphthiocerol dimycocerosate esters (PDIM) synthesis. Can directly transfer the mycocerosate bound to the mycocerosic acid synthase (mas) onto the substrate alcohols. Is also able to catalyze acyl transfer using various nucleophiles as acceptors and several acyl-CoA thioesters as donors in vitro; preference is observed for saturated medium chain alcohols and long [...] (422 aa) |
| | mas | Rv2940c, (MTCY24G1.09, MTCY19H9.08c), len: 2111 aa. Probable mas, mycocerosic acid synthase membrane associated, multifunctional enzyme (see citations below),almost identical to Q02251|MCAS_MYCBO|mas mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 13226, E(): 0, (95.8% identity in 2115 aa overlap) (see Mathur & Kolattukudy 1992); and equivalent to Q9CD78|mas|ML0139 putative mycocerosic synthase from Mycobacterium leprae (2116 aa), FASTA scores: opt: 12142,E(): 0, (87.95% identity in 2119 aa overlap); and Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable myc [...] (2111 aa) |
| | fadD28 | Fatty-acid-AMP ligase FadD28 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Catalyzes the activation of long-chain fatty acids (C22-24 fatty acids) as acyl-adenylates (acyl-AMP), which are then transferred to the multifunctional polyketide synthase Mas for further chain extension. Involved in the biosynthesis of mycoserates. Probably plays a role in host phagosome maturation arrest. Belongs to the ATP-dependent AMP-binding enzyme family. (580 aa) |
| | pks1 | Probable polyketide synthase Pks1; May play a role in phthiocerol biosynthesis. (1616 aa) |
| | pks15 | Rv2947c, (MTCY24G1.02), len: 496 aa. Probable pks15,polyketide synthase. Almost identical to G560508|Q50469 PKS002B protein from Mycobacterium tuberculosis (495 aa),FASTA scores: opt: 3270, E(): 0, (99.6% identity in 496 a a overlap). Similar to Mycobacterium tuberculosis proteins MTCY338.20|RV2931|PPSA_MYCTU ppsA phenolpthiocerol synthesis (1876 aa) (49.9% identity in 465 aa overlap); MTCY24G1.09|RV2940C|P96291 Putative mas, mycocerosic acid synthase (2111 aa) (50.2% identity in 454 aa overlap); and MTCY22H8.03|RV2382C|P71718 hypothetical protein (444 aa) (47.6% identity in 437 aa ove [...] (496 aa) |
| | Rv3026c | Rv3026c, (MTV012.41c), len: 304 aa. Conserved hypothetical protein, similar to Q9RCZ0|SCM10.08C putative acyltransferase from Streptomyces coelicolor (275 aa),FASTA scores: opt: 393, E(): 2.2e-17, (41.4% identity in 299 aa overlap). Similar in part to other hypothetical proteins and acyltransferases e.g. BAB51968|MLR5533 from Rhizobium loti (266 aa), FASTA scores: opt: 280, E(): 2.4e-10, (29.45% identity in 258 aa overlap); Q9KIH9 putative acyltransferase (putative acyltransferase transmembrane protein) from Rhizobium meliloti (Sinorhizobium meliloti) (292 aa), FASTA scores: opt: 252,E [...] (304 aa) |
| | Rv3034c | Possible transferase; Putative acetyltransferase that is probably involved in the biosynthesis of 6-O-methylglucosyl lipopolysaccharides (MGLP). (300 aa) |
| | Rv3087 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity. (472 aa) |
| | tgs4 | Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs4; Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmitoyl-CoA. (474 aa) |
| | fadD13 | Probable chain-fatty-acid-CoA ligase FadD13 (fatty-acyl-CoA synthetase); Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. Catalyzes the activation of long-chain fatty acids as acyl-coenzyme A (acyl-CoA), which are then transferred to the multifunctional polyketide synthase (PKS) type III for further chain extension. It has preference for the fatty acid with long chain length in the following order: hexacosanoic acid (C26), tetracosanoic acid (C24) and palmitic acid (C16); Belongs to the ATP-dependent AMP-bi [...] (503 aa) |
| | tgs1 | Triacylglycerol synthase (diacylglycerol acyltransferase) Tgs1; Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (Probable). (463 aa) |
| | Rv3225c | GCN5-related N-acetyltransferase, phosphorylase; Rv3225c, (MTCY07D11.01), len: 474 aa. Conserved hypothetical protein has GNAT (Gcn5-related N-acetyltransferase) domain in N-terminal part (see Vetting et al. 2005) and phosphotransferase domain in C-terminal part. C-terminal part shows similarity to various bacterial phosphotransferases e.g. BAB49093|MLL1809 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (298 aa),FASTA scores: opt: 557, E(): 2.8e-26, (34.55% identity in 295 aa overlap); P14509|KKA8_ECOLI|APHA aminoglycoside 3'-phosphotransferase from Escherichia coli (271 [...] (474 aa) |
| | Rv3233c | Rv3233c, (MTCY20B11.08c), len: 196 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to C-terminus of Q9RIU8|SCM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 308, E(): 1.2e-12, (32.0% identity in 200 aa overlap); and several hypothetical M. tuberculosis proteins e.g. O06343|YY80_MYCTU|Rv3480c|MTCY13E12.33c (497 aa),FASTA scores: opt: 248, E(): 9.8e-09, (27.5% identity in 200 aa overlap); MTCY28_26; MTCY493_29; MTCY31_25; MTCY31_25. (196 aa) |
| | tgs3 | Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs3; Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). Belongs to the long-chain O-acyltransferase family. (271 aa) |
| | metA | Homoserine O-acetyltransferase; Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine. (379 aa) |
| | Rv3371 | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (446 aa) |
| | gcp | Probable O-sialoglycoprotein endopeptidase Gcp (glycoprotease); Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. (344 aa) |
| | rimI | N-alpha-acetyltransferase RimI; N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues. Able to mediate acetylation of a wide variety of N-terminal residues, with preference for hydrophobic N- termini. Acetylates GroS/GroES and GroEL1. Able to acetylate the ribosomal protein S18, but it is unclear whether it acetylates its N- terminal alanine residue; Belongs to the acetyltransferase family. RimI subfamily. (158 aa) |
| | mhpE | Rv3469c, (MTCY13E12.22c), len: 336 aa. Probable mhpE, 4-hydroxy-2-oxovalerate aldolase, similar to others (principally from Pseudomonas species) e.g. Q99PZ1|SCP1.301|SCP1.53c from Streptomyces coelicolor (338 aa), FASTA scores: opt: 615, E(): 7.9e-31, (37.65% identity in 332 aa overlap); Q9X9Q0|NIKB NIKB protein (see Bruntner et al., 1999) from Streptomyces tendae (357 aa), FASTA scores: opt: 571, E(): 4.4e-28, (34.5% identity in 339 aa overlap); P51014|BPHF_PSES1 from Pseudomonas sp. strain KKS102 (352 aa), FASTA scores: opt: 549, E(): 9.9e-27,(31.2% identity in 314 aa overlap); Q5198 [...] (336 aa) |
| | Rv3480c | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Upon expression in E.coli has a weak triacylglycerol synthase function, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions weakly as a wax synthase, as it incorporates palmityl alcohol into wax esters in the presence of palmitoyl-CoA. (497 aa) |
| | ltp4 | Rv3522, (MTV023.29), len: 354 aa. Possible ltp4,lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30103|AF0134 3-ketoacyl-CoA thiolase (ACAB-4) from Archaeoglobus fulgidus (398 aa) FASTA scores: opt: 352, E(): 5.3e-15, (30.45% identity in 381 aa overlap); O29295|AF0967 3-ketoacyl-CoA thiolase (ACAB-9) from Archaeoglobus fulgidus (400 aa) FASTA scores: opt: 312,E(): 1.8e-12, (28.05% identity in 367 aa overlap); O29294|AF0968 3-ketoacyl-CoA thiolase (ACAB-10) from Archaeoglobus fulgidus (388 aa), FASTA scores: opt: 293,E(): 2.9e-11, (25.9% identity in 309 aa overl [...] (354 aa) |
| | ltp3 | Rv3523, (MTCY03C7.33c), len: 394 aa. Probable ltp3,lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30037|AF0202 3-ketoacyl-CoA thiolase (ACAB-6) from Archaeoglobus fulgidus (380 aa) FASTA scores: opt: 782, E(): 1.7e-40, (38.35% identity in 386 aa overlap); Q9Y9A1|APE2384 long hypothetical non specific lipid-transfer protein (acethyl CoA synthetase) from Aeropyrum pernix (394 aa), FASTA scores: opt: 626, E(): 5.9e-31, (35.75% identity in 386 aa overlap); BAB59210|TVG0067506 lipid transfer protein from Thermoplasma volcanium (390 aa), FASTA scores: opt: 591,E(): [...] (394 aa) |
| | hsaF | Probable 4-hydroxy-2-oxovalerate aldolase (HOA); Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta- cleavage pathway for the degradation of aromatic compounds. Belongs to the 4-hydroxy-2-oxovalerate aldolase family. (346 aa) |
| | ltp2 | Rv3540c, (MTCY03C7.16), len: 386 aa. Probable ltp2,lipid-transfer protein or keto acyl-CoA thiolase, similar to several e.g. Q9X4X2|DITF DITF protein (hypothetical protein, similar to non-specific lipid-transfer protein and 3-ketoacyl-CoA thiolase) from Pseudomonas abietaniphila (397 aa), FASTA scores: opt: 665, E(): 5.3e-34, (33.4% identity in 392 aa overlap); O30255|AF2416 3-ketoacyl-CoA thiolase (ACAB-12) from Archaeoglobus fulgidus (384 aa),FASTA scores: opt: 496, E(): 1.6e-23, (30.35% identity in 389 aa overlap); O28978|AF1291 3-ketoacyl-CoA thiolase (ACAB-11) from Archaeoglobus f [...] (386 aa) |
| | fadA5 | Probable acetyl-CoA acetyltransferase FadA5 (acetoacetyl-CoA thiolase); Involved in the beta-oxidation of the cholesterol side chain. It is important for utilization of cholesterol as a sole carbon source in vitro and for full virulence in the chronic stage of mouse lung infection. Catalyzes the thiolysis of 3,22-dioxochol-4-en-24-oyl-CoA to yield 3-oxo-4-pregnene-20-carboxyl- CoA (3-OPC-CoA) and acetyl-CoA. Also able to use acetoacetyl-CoA (AcAcCoA) as substrate. (391 aa) |
| | fadA6 | Rv3556c, (MTCY06G11.03), len: 386 aa. Probable fadA6, acetyl-CoA acetyltransferase, similar to many e.g. Q9K409|2SCG61.06c from Streptomyces coelicolor (389 aa),FASTA scores: opt: 1091, E(): 2.9e-58, (48.1% identity in 399 aa overlap); Q9AAT4|CC0510 from Caulobacter crescentus (391 aa), FASTA scores: opt: 902, E(): 6.6e-47, (40.25% identity in 395 aa overlap); P45359|THL_CLOAB from Clostridium acetobutylicum (392 aa), FASTA scores: opt: 872, E(): 4.2e-45, (37.9% identity in 396 aa overlap); Q9I2A8|ATOB|PA2001 from Pseudomonas aeruginosa (393 aa),FASTA scores: opt: 872, E(): 4.2e-45, (4 [...] (386 aa) |
| | nat | Arylamine N-acetyltransferase Nat (arylamine acetylase); Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines. Is able to utilize not only acetyl-CoA, but also n- propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate. As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species. (283 aa) |
| | lsr2 | Iron-regulated H-NS-like protein Lsr2; DNA-bridging protein that has both architectural and regulatory roles. Influences the organization of chromatin and gene expression by binding non-specifically to DNA, with a preference for AT-rich sequences, and bridging distant DNA segments. Binds in the minor groove of AT-rich DNA. Represses expression of multiple genes involved in a broad range of cellular processes, including major virulence factors or antibiotic-induced genes, such as iniBAC or efpA , and genes important for adaptation of changing O(2) levels. May also activate expression of [...] (112 aa) |
| | leuA | 2-isopropylmalate synthase LeuA (alpha-isopropylmalate synthase) (alpha-IPM synthetase) (IPMS); Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate). (644 aa) |
| | tgs2 | Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs2; Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (454 aa) |
| | Rv3740c | Possible triacylglycerol synthase (diacylglycerol acyltransferase); Upon expression in E.coli has a weak triacylglycerol synthase function, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions weakly as a wax synthase, as it incorporates palmityl alcohol into wax esters in the presence of palmitoyl-CoA. (448 aa) |
| | pks13 | Polyketide synthase Pks13; Rv3800c, (MTV026.05c), len: 1733 aa. Probable pks13,polyketide synthase, equivalent to Q9CDB1|PKS13|ML0101 polyketide synthase from Mycobacterium leprae (1784 aa),FASTA scores: opt: 7454, E(): 0, (83.6% identity in 1748 aa overlap); and similar to Q9Z5K6|ML2357|MLCB12.02c putative polyketide synthase from Mycobacterium leprae (1871 aa),FASTA scores: opt: 1682, E(): 1.2e-85, (38.3% identity in 1096 aa overlap). Also similar in part to many e.g. Q9ADL6|SORA soraphen polyketide synthase a from Polyangium cellulosum (6315 aa) FASTA scores: opt: 1422, E(): 1e-70,( [...] (1733 aa) |
| | fbpD | MPT51/MPB51 antigen; May have a role in host tissue attachment, whereby ligands may include the serum protein fibronectin and small sugars. (299 aa) |
| | fbpA | Diacylglycerol acyltransferase/mycolyltransferase Ag85A; The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha- trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-treh [...] (338 aa) |
| | Rv3814c | Rv3814c, (MTCY409.16), len: 261 aa. Possible acyltransferase, highly similar to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa), FASTA scores: opt: 753, E(): 7.7e-42, (46.75% identity in 246 aa overlap). Also highly similar to many acyltransferases and hypothetical proteins e.g. Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa),FASTA scores: opt: 587, E(): 4.6e-31, (41.95% identity in 243 aa overlap); Q9ZBS1|SC7A1.02 putative acyltransferase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 293, E(): 6.6e-12, (29.2% identit [...] (261 aa) |
| | Rv3815c | Rv3815c, (MTCY409.15), len: 251 aa. Possible acyltransferase, highly similar to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa), FASTA scores: opt: 845, E(): 2.7e-47, (53.25% identity in 246 aa overlap). Also highly similar to Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa),FASTA scores: opt: 656, E(): 3.7e-35, (47.85% identity in 234 aa overlap); and similar to many putative acyltransferases and hypothetical proteins e.g. P74498|SLL1848 hypothetical 24.3 KDA protein from Synechocystis sp. strain PCC 6803 (225 aa) FASTA scores: o [...] (251 aa) |
| | Rv3816c | Rv3816c, (MTCY409.14), len: 259 aa. Possible acyltransferase, equivalent to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa) FASTA scores: opt: 1401, E(): 1.5e-80, (81.9% identity in 254 aa overlap). Also highly similar to many putative acyltransferases and hypothetical proteins e.g. Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa), FASTA scores: opt: 758, E(): 2.4e-40,(51.7% identity in 234 aa overlap); Q9ZBS1|SC7A1.02 putative acyltransferase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 312, E(): 2e-12, (29.55% ident [...] (259 aa) |
| | papA2 | Possible conserved polyketide synthase associated protein PapA2; Catalyzes the acylation of trehalose-2-sulfate by adding the palmitoyl group at the 2'-position to yield the intermediate trehalose- 2-sulfate-2'-palmitate (SL659) during the cell wall sulfolipid-1 (SL-1) biosynthesis; Belongs to the PapA acyltransferase family. (468 aa) |
| | chp1 | Conserved hypothetical protein; Involved in the final steps of the cell wall sulfolipid-1 (SL-1) biosynthesis. Catalyzes two successive acylations of the precursor 2-palmitoyl-3-(C43)-phthioceranyl-alpha, alpha'-D-trehalose- 2'-sulfate (SL1278) to yield the tetraacylated sulfolipid SL-1. (404 aa) |
| | papA1 | Conserved polyketide synthase associated protein PapA1; Catalyzes the acylation of trehalose-2-sulfate-2'-palmitate (SL659) by adding the (hydroxy)phthioceranoyl group at the 3'-position to yield the diacylated intermediate 2-palmitoyl-3-(C43)-phthioceranyl- alpha, alpha'-D-trehalose-2'-sulfate (SL1278) during the cell wall sulfolipid-1 (SL-1) biosynthesis; Belongs to the PapA acyltransferase family. (511 aa) |
| | pks2 | Polyketide synthase Pks2; Catalyzes the synthesis of the hepta- and octamethyl phthioceranic acids and/or hydroxyphthioceranic acids that are the major acyl constituents of sulfolipids. (2126 aa) |
