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| | hsdM | Rv2756c, (MTV002.21c), len: 540 aa. Possible hsdM,type I restriction/modification system DNA methylase (M protein), highly similar to others e.g. Q9P9X8|XF2742 from Xylella fastidiosa (519 aa), FASTA scores: opt: 1613, E(): 1.9e-96, (52.3% identity in 543 aa overlap); O34139|HSDM from Klebsiella pneumoniae (539 aa), FASTA scores: opt: 1267, E(): 4.4e-74, (45.9% identity in 549 aa overlap); P72418|sty|SBLI|HSDM from Salmonella typhimurium (539 aa),FASTA scores: opt: 1263, E(): 8e-74, (45.7% identity in 549 aa overlap); etc. Possible alternative start site (GTG) overlapping with terminat [...] (540 aa) |
| | thyX | Probable thymidylate synthase ThyX (ts) (TSase); Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant. Is essential for growth of the pathogen on solid media in vitro; the essential function is something other than dTMP synthase. (250 aa) |
| | Rv2751 | Conserved protein; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (296 aa) |
| | Rv2689c | Conserved alanine and valine and glycine rich protein; Rv2689c, (MTCY05A6.10c), len: 405 aa (other less probable starts possible). Conserved ala-, val-, gly-rich protein, similar to O54099|SC10A5.06 hypothetical 49.5 KDA protein from Streptomyces coelicolor (458 aa), FASTA scores: opt: 455, E(): 2.7e-20, (38.35% identity in 417 aa overlap); and shows weak similarity in part with several methyltransferases e.g. Q9X0H9|TM1094 putative RNA methyltransferase from Thermotoga maritima (439 aa), FASTA scores: opt: 306, E(): 3e-11, (25.9% identity in 436 aa overlap); AK79403|CAC1435 S-adenosyl [...] (405 aa) |
| | Rv2622 | Rv2622, (MTCY01A10.10c), len: 273 aa. Possible methyltransferase, similar in part to others e.g. AAK75664|SP1578 putative methyltransferase from Streptococcus pneumoniae (252 aa), FASTA scores: opt: 406,E(): 6.6e-18, (32.65% identity in 251 aa overlap); Q9F8B8 methyltransferase from Streptococcus agalactiae (254 aa),FASTA scores: opt: 381, E(): 2.3e-16, (31.75% identity in 252 aa overlap); Q9RJB6|SCF91.08 putative methyltransferase from Streptomyces coelicolor (231 aa), FASTA scores: opt: 159, E(): 0.0091, (33.1% identity in 151 aa overlap); etc. Also similar in part to several hypothe [...] (273 aa) |
| | mmuM | Rv2458, (MTV008.14), len: 302 aa. Probable mmuM,homocysteine S-methyltransferase, equivalent to Q9CBY5|ML1478 possible transferase from Mycobacterium leprae (293 aa), FASTA scores: opt: 1507, E(): 2.7e-86,(78.85% identity in 293 aa overlap). Also similar to others e.g. Q47690|MMUM_ECOLI|B0261 homocysteine S-methyltransferase from Escherichia coli strain K12 (310 aa), FASTA scores: opt: 863, E(): 2.4e-46, (47.65% identity in 298 aa overlap); Q9FUM7 homocysteine S-methyltransferase-4 from Zea mays (Maize) (342 aa), FASTA scores: opt: 324, E(): 6.8e-13, (44.45% identity in 306 aa overlap) [...] (302 aa) |
| | rsmE | Conserved hypothetical protein; Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity); Belongs to the RNA methyltransferase RsmE family. (262 aa) |
| | Rv2258c | Rv2258c, (MTV022.08c), len: 353 aa. Possible transcriptional regulatory protein, similar to several hypothetical proteins from C. elegans. FASTA scores: sptr|O01593|O01593 coded for by C. elegans CDNA YK102 F (365 aa) opt: 577, E(): 6.4e-31; (30.5% identity in 341 aa overlap). Contains possible helix-turn helix motif at aa 47-68 (+3.65 SD). (353 aa) |
| | rsmH | Conserved protein; Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA; Belongs to the methyltransferase superfamily. RsmH family. (396 aa) |
| | metH | Methionine synthase; Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate (By similarity); Belongs to the vitamin-B12 dependent methionine synthase family. (1192 aa) |
| | trmI | RNA methyltransferase; Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. (280 aa) |
| | cobL | Precorrin-6Y C(5,15)-methyltransferase (decarboxylating) CobL; Catalyzes the methylation of both C-5 and C-15 in precorrin- 6Y to form precorrin-8X. (390 aa) |
| | cobM | Precorrin-3 methylase CobM (precorrin-4 C11-methyltransferase); Catalyzes the methylation of C-11 in precorrin-4 to form precorrin-5. (251 aa) |
| | Rv2067c | Conserved protein; Rv2067c, (MTCY49.06c), len: 407 aa. Conserved protein, similar to many. (407 aa) |
| | cobI | Cobalamin biosynthesis protein CobIJ; Methylates precorrin-2 at the C-20 position to produce precorrin-3A. (508 aa) |
| | erm(37) | Rv1988, (MTCY39.31c), len: 179 aa. Probable erm(37),23S rRNA methyltransferase, similar to ERME_SACER|P07287 rrna adenine n-6-methyltransferase (370 aa), FASTA scores: opt: 259, E(): 2e-11, (35.1% identity in 171 aa overlap); contains PS00092 N-6 Adenine-specific DNA methylases signature. Also similar to Mycobacterium tuberculosis Rv1010 ksgA 16S rRNA dimethyltransferase. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007); Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. (179 aa) |
| | Rv1896c | Conserved hypothetical protein; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (303 aa) |
| | Rv1889c | Rv1889c, (MTCY180.29), len: 118 aa. Conserved hypothetical protein. Part of large family of Mycobacterium tuberculosis proteins with conserved N-terminal domain of ~120 aa. Includes: Rv3399|Q50726|MTCY78.29C conserved hypothetical protein (348 aa), FASTA results: opt: 504,E(): 7.3e-29, (64.2% identity in 120 aa overlap); Rv0726c|P95074; Rv0731c; etc. Rv1888A possibly continuation of this CDS. (118 aa) |
| | Rv1888A | Rv1888A, len: 57 aa. Conserved hypothetical protein. Possibly continuation of Rv1889c, part of large family of Mycobacterium tuberculosis proteins with conserved N-terminal domain of ~ 120 aa. Includes: C-terminus of Rv0726c|P95074 conserved hypothetical protein (367 aa),FASTA scores: opt: 295, E(): 3.1e-15, (73.684% identity in 57 aa overlap); C-terminus of Rv3399|Q50726|MTCY78.29c conserved hypothetical protein (348 aa), FASTA scores: opt: 504, E(): 7.3e-29, (64.2% identity in 120 aa overlap); C-terminus of Rv0731c; etc. (57 aa) |
| | Rv1729c | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (312 aa) |
| | Rv1703c | Rv1703c, (MTCI125.25c), len: 196 aa. Probable catechol-o-methyltransferase, most similar to COMT_HUMAN|P21964 soluble form of mammalian catechol o-methyltransferase (271 aa), FASTA scores: opt: 405, E(): 7.8e-29, (38.9% identity in 190 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical methyltransferases Rv0187, Rv1220c. (196 aa) |
| | tlyA | 2'-O-methyltransferase TlyA; Acts as a host evasion factor, that significantly contributes to the pathogenesis of M.tuberculosis by modulating adaptive immune responses by inhibiting host-protective Th1 and Th17 cytokine responses as well as autophagy. Catalyzes the 2'-O-methylation at nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA. Is likely involved in ribosomal biogenesis. Also exhibits hemolytic activity in vitro, by binding with and oligomerizing into host cell membranes. Belongs to the TlyA family. (268 aa) |
| | tsnR | Rv1644, (MTCY06H11.08), len: 260 aa. Possible tsnR,23S rRNA methyltransferase, similar to several e.g. TSNR_STRLU|P52393 from Streptomyces laurentii (270 aa),FASTA scores: opt: 276, E(): 3.6e-11, (27.6% identity in 261 aa overlap). Also similar to M. tuberculosis hypothetical proteins Rv0881, Rv3579c, and Rv0380c; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (260 aa) |
| | Rv1523 | Probable methyltransferase; Rv1523, (MTCY19G5.05c), len: 347 aa (start uncertain). Probable methyltransferase, similar to G560513|U0002O Mycobacterium leprae (270 aa), FASTA scores: opt: 965, E(): 0, (60.3% identity in 247 aa overlap). Also similar to many e.g. Q54303|X86780 methyltransferase RAPM from Streptomyces hygroscopicus (317 aa), FASTA scores: opt: 323, E(): 1e-15, (41.2% identity in 136 aa overlap). And similar to M. tuberculosis hypothetical proteins Rv2952, Rv1405c, Rv1403c, Rv0839. (347 aa) |
| | Rv1506c | Hypothetical protein; Probably plays a role in host phagosome maturation arrest, as well as a role in the synthesis of acyltrehalose-containing glycolipids ; Belongs to the methyltransferase superfamily. (166 aa) |
| | Rv1498c | Rv1498c, (MTCY277.20c), len: 205 aa. Probable methyltransferase. Similar to G2792343|AF040571 methyltransferase from amycolatopsis mediterranei (272 aa),FASTA scores: E(): 5.1e-11, (32.3% identity in 124 aa overlap). Contains PS00017 ATP/GTP-binding site motif A. (205 aa) |
| | fmu | Probable Fmu protein (sun protein); May act as RNA methyltransferase. (457 aa) |
| | alkA | Probable bifunctional transcriptional activator/DNA repair enzyme AlkA; Is involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs the Sp diastereomer of DNA methylphosphotriester lesions by a direct and irreversible transfer of the methyl group to one of its own cysteine residues. Also catalyzes the hydrolysis of the deoxyribose N-glycosidic bond to excise 3- methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions (By similarity); In the C-terminal sect [...] (496 aa) |
| | ogt | Methylated-DNA--protein-cysteine methyltransferase; Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. (165 aa) |
| | hemK | Probable HemK protein homolog HemK; Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif. (325 aa) |
| | Rv1220c | Probable methyltransferase; Rv1220c, (MTCI61.03c), len: 215 aa. Possible methyltransferase, some similarity to MDMC_STRMY|Q00719 o-methyltransferase from Streptomyces mycarofaciens (221 aa), FASTA scores; opt: 289, E(): 1.3e-07, (30.0% identity in 203 aa overlap). Also similar to Mycobacterium tuberculosis methyltransferases Rv0187|MTCI28.26 (32.9% identity in 222 aa overlap) and Rv1703c. Start site chosen by homology; other possible start sites exist upstream. (215 aa) |
| | omt | Rv1153c, (MTCI65.20c), len: 282 aa. Probable omt,O-methyltransferase, similar to TCMP_STRGA|P39887 Tetracenomycin polyketide synthesis O-methyltransferase tcmP from Streptomyces glaucescens (270 aa), FASTA scores: opt: 368, E(): 1.7e-17, (31.3% identity in 233 aa overlap). (282 aa) |
| | Rv1147 | Conserved protein; Rv1147, (MTCI65.14), len: 216 aa. Conserved protein,similar to many conserved hypothetical proteins, and some similarity to several methyltransferases e.g. Q05197|PMTA_RHOSH phosphatidylethanolamine N-methyltransferase from R. sphaeroides (203 aa), FASTA scores: opt: 156, E(): 0.00073, (27.6% identity in 156 aa overlap). (216 aa) |
| | Rv1139c | Rv1139c, (MTCI65.06c), len: 166 aa. Conserved hypothetical membrane protein. Highly similar to P54158|YBPQ_BACSU hypothetical Bacillus subtilis protein,YBPQ (168 aa), FASTA scores: opt: 446, E(): 2.2e-26, (38.4% identity in 164 aa overlap). Some similarity to Mycobacterium tuberculosis hypothetical proteins, Rv0740,Rv0750. (166 aa) |
| | metE | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. (759 aa) |
| | ksgA | Ribosomal RNA small subunit methyltransferase A; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. (317 aa) |
| | rsmI | Conserved protein; Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA; Belongs to the methyltransferase superfamily. RsmI family. (285 aa) |
| | Rv0893c | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (325 aa) |
| | Rv0881 | Rv0881, (MTCY31.09), len: 288 aa. Possible rRNA methyltransferase, highly similar to others and hypothetical proteins e.g. CAB76071.1|AL157953 putative rRNA methylase from Streptomyces coelicolor (272 aa); NP_421117.1|NC_002696 spoU rRNA methylase family protein from Caulobacter crescentus (268 aa); D90913_93|P74261 rRNA methylase from Synechocystis sp. (274 aa), FASTA scores: E(): 1.1e-13, (26.3% identity in 278 aa overlap); P18644|TSNR_STRCN rRNA methyltransferase from Streptomyces cyaneus (Streptomyces curacoi) (269 aa), FASTA scores: E(): 3.7e-08, (23.9% identity in 268 aa overlap) [...] (288 aa) |
| | Rv0830 | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (301 aa) |
| | Rv0731c | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (318 aa) |
| | Rv0726c | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (367 aa) |
| | Rv0725c | Conserved hypothetical protein; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (301 aa) |
| | mmaA1 | Mycolic acid methyltransferase MmaA1; Involved in the conversion of a cis-olefin into a trans- olefin with concomitant introduction of an allylic methyl branch at the proximal position of the precursor to both the methoxy and ketomycolic acids. It directly affects the cis- to trans ratio and indirectly affects the keto to methoxy ratio; Belongs to the CFA/CMAS family. (286 aa) |
| | mmaA2 | Cyclopropane mycolic acid synthase MmaA2; Catalyzes the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. MmaA2 also catalyzes the biosynthesis of the cis-cyclopropanated methoxymycolates. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence. (287 aa) |
| | mmaA3 | Methoxy mycolic acid synthase MmaA3; Involved in the biosynthesis of methoxymycolic acid. It catalyzes the O-methylation of the hydroxy group of the hydroxymycolate to form a methyl ether; Belongs to the CFA/CMAS family. (293 aa) |
| | mmaA4 | Hydroxymycolate synthase MmaA4; Involved in the biosynthesis of hydroxymycolate, a common precursor of oxygenated mycolic acids (methoxy-mycolate and keto- mycolate). Probably transfers a methyl group from the S- adenosylmethionine (SAM) cofactor and, subsequently or simultaneously, a water molecule onto the double bound of ethylene substrates, leading to the formation of the hydroxylated product at the distal position. Involved in the activation of the antitubercular drug thiacetazone (TAC). (301 aa) |
| | Rv0567 | Rv0567, (MTV039.05), len: 339 aa. Probable methyltransferase, similar to several e.g. P39896|TCMO_STRGA tetracenomycin polyketide synthesis 8-O-methyltransferase from Streptomyces glaucescens (339 aa), FASTA scores: opt: 685, E(): 0, (35.8% identity in 335 aa overlap); P10950|HIOM_BOVIN hydroxyindole O-methyltransferase from Bos taurus (345 aa), FASTA scores: opt: 509, E(): 3.4e-27, (30.7% identity in 332 aa overlap) etc. (339 aa) |
| | Rv0560c | Rv0560c, (MTCY25D10.39c), len: 241 aa. Possible benzoquinone methyltransferase (see citation below),similar to other hypothetical proteins and methyltransferases e.g. Q54300 methyltransferase (211 aa),FASTA scores: opt: 203, E(): 4.8e-07, (30.9% identity in 136 aa overlap). Similar to Rv3699, Rv1377c, Rv2675c, etc from Mycobacterium tuberculosis. Rv0560c can be induced by salicylate and para-amino-salicylate (pas). (241 aa) |
| | menH | Demethylmenaquinone methyltransferase; Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2); Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family. (234 aa) |
| | Rv0521 | Possible methyltransferase/methylase (fragment); Rv0521, (replaces MTCY20G10.11), len: 101 aa. Possible fragment of methyltransferase (possibly second part), highly similar to C-terminus of several methyltransferases e.g. AAF87203.1|AF216282 sarcosine-dimethylglycine methyltransferase from Halorhodospira halochloris (279 aa). Possibly continuation of Rv0520 but we can find no frameshift to account for this. (101 aa) |
| | Rv0520 | Possible methyltransferase/methylase (fragment); Rv0520, (MTCY20G10.10), len: 116 aa. Possible fragment of methyltransferase (possibly first part), highly similar to part of several methyltransferases e.g. Q43445|U43683 S-adenosyl-L-methionine:DELTA24-sterol-C-methyltransferase from Glycine max (Soybean)(367 aa), FASTA scores: opt: 190,E(): 2.3e-12, (39.2% identity in 74 aa overlap). Also some similarity to MTCY19G5_5 from Mycobacterium tuberculosis. Possibly continues as Rv0521 but we can find no frameshift to account for this. (116 aa) |
| | Rv0146 | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (310 aa) |
| | Rv0187 | Probable O-methyltransferase; Catechol O-methyltransferase that can use various catechol- like compounds such as gallic acid (GA), 3,4-dihydroxy-5-methoxy- benzoic acid (5OMeBA), protocatechuic acid (PCA), 3,4-dihydroxy- benzaldehyde (DHA), dopamine, caffeic acid (CA), luteolin, quercetin, and 5-hydroxyuridine; Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family. (220 aa) |
| | trmB | Hypothetical methlytransferase (methylase); Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. (263 aa) |
| | Rv0213c | Rv0213c, (MTCY08D5.08c), len: 437 aa. Possible methyltransferase, weakly similar to others methyltransferases e.g. AF127374_30|LINA from Streptomyces lavendulae (611 aa), FASTA scores: opt: 400, E(): 8.1e-19,(27.3% identity in 388 aa overlap); Q50258 fortimicin kl1 methyltransferase (553 aa), FASTA scores: opt: 267, E(): 1.2e-13, (29.3% identity in 351 aa overlap). (437 aa) |
| | Rv0224c | Rv0224c, (MTCY08D5.19c), len: 254 aa. Possible methyltransferase, showing weak similarity with other methyltransferases e.g. P74388 sterol-C-methyltransferase (318 aa), FASTA scores: opt: 190, E(): 3.6e-05, (33.3% identity in 114 aa overlap). Equivalent to AL022486|MLCB1883_1 from Mycobacterium leprae (269 aa),FASTA scores: opt: 1456, E(): 0, (82.9% identity in 252 aa overlap). Also some similarity with MTCY21B4.22c from Mycobacterium tuberculosis FASTA score: (30.1% identity in 136 aa overlap). (254 aa) |
| | Rv0281 | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (302 aa) |
| | tam | Probable trans-aconitate methyltransferase Tam; Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate. (261 aa) |
| | Rv0380c | Rv0380c, (MTV036.15c), len: 183 aa. Possible RNA methyltransferase, equivalent to CAC32002.1|AL583925 possible RNA methyltransferase from Mycobacterium leprae (182 aa). Also some similarity with others methyltransferases e.g. P19396|TRMH_ECOLI|78514|JV0043 tRNA (guanosine-2'-O-)-methyltransferase (tRNA methyltransferase) from Escherichia coli (229 aa), FASTA scores: opt: 227, E(): 1.4e-09, (28.9% identity in 166 aa overlap). Also similar to Rv0881, Rv3579c, Rv1644 from Mycobacterium tuberculosis. (183 aa) |
| | ufaA1 | Tuberculostearic acid methyltransferase UfaA1; Involved in the biosynthesis of the tuberculostearic acid (10-methylstearic-acid or TSA), a constituent lipid of the mycobacterial cell wall. Catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (SAM) to the double bond of oleic acid in phosphatidylethanolamine or phosphatidylcholine to produce TSA. Belongs to the CFA/CMAS family. (427 aa) |
| | umaA | Possible mycolic acid synthase UmaA; Methyltransferase that modifies short-chain fatty acids. In vitro, catalyzes the transfer of the methyl group from S-adenosyl-L- methionine (SAM) to the double bond of phospholipid-linked oleic acid to produce tuberculostearic acid (10-methylstearic-acid or TSA). Belongs to the CFA/CMAS family. (286 aa) |
| | pcaA | Mycolic acid synthase PcaA (cyclopropane synthase); Involved in the phagosome maturation block (PMB). Catalyzes the conversion of a double bond to a cyclopropane ring at the proximal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. It can use cis, cis 11,14-eicosadienoic acid and linoelaidic acid as substrate. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence. (287 aa) |
| | cmaA2 | Cyclopropane mycolic acid synthase 2; Catalyzes the formation of trans cyclopropanated ketomycolate or methoxymycolate through the conversion of a double bond to a cyclopropane ring at the proximal position of an oxygenated mycolic acid via the transfer of a methylene group from S-adenosyl-L- methionine. In the absence of MmaA2, CmaA2 has a non-specific cis- cyclopropanating activity and is able to catalyze the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid. Cyclopropanated mycolic acids are key factors participating in cell envel [...] (302 aa) |
| | hemD | Uroporphyrinogen III methyltransferase / synthase; Rv0511, (MTCY21C8.02), len: 565 aa. Probable hemD (alternate gene name: cysG), uroporphyrin-III C-methyltransferase, highly similar to others e.g. CAC31936.1|AL583925 possible uroporphyrin-III C-methyltransferase from Mycobacterium leprae (563 aa); and S72909|CYSG from Mycobacterium leprae (472 aa), FASTA scores: opt: 1946, E(): 0, (83.3% identity in 472 aa overlap); T36265|5123662|CAB45351.1|AL079345 probable uroporphyrin-III C-methyltransferase from Streptomyces coelicolor (565 aa); and similar to others e.g. AAK00606.1|AF221100_3|AF [...] (565 aa) |
| | Rv0089 | Rv0089, (MTCY251.07), len: 197 aa. Possible methyltransferase, showing some weak similarity to others. Also some similarity with many biotin biosynthesis proteins. Belongs to the methyltransferase superfamily. (197 aa) |
| | Rv0145 | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (317 aa) |
| | gid | Probable glucose-inhibited division protein B Gid; Specifically methylates the N7 position of guanine in position 518 of 16S rRNA. (224 aa) |
| | Rv3787c | Conserved hypothetical protein; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (308 aa) |
| | Rv3767c | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (314 aa) |
| | Rv3720 | Possible fatty acid synthase; May be a S-adenosylmethionine-dependent methyltransferase involved in fatty acid metabolism. (420 aa) |
| | egtD | Conserved hypothetical protein; Catalyzes the SAM-dependent triple methylation of the alpha- amino group of histidine to form hercynine, a step in the biosynthesis pathway of ergothioneine; Belongs to the methyltransferase superfamily. EgtD family. (321 aa) |
| | Rv3579c | Rv3579c, (MTCY06G11.26c), len: 322 aa. Possible tRNA/rRNA methyltransferase, equivalent, but longer 31 aa,to Q9CCW4|ML0324 putative methyltransferase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1517,E(): 3.4e-79, (83.75% identity in 277 aa overlap). Also highly similar to Q9L0Q5|SCD8A.09 from Streptomyces coelicolor (314 aa), FASTA scores: opt: 937, E(): 3.4e-46,(56.75% identity in 319 aa overlap); and similar to others e.g. Q06753|YACO_BACSU from Bacillus subtilis (249 aa),FASTA scores: opt: 616, E(): 4.9e-28, (41.05% identity in 246 aa overlap); Q9KGF2|BH0113 from Bacillus [...] (322 aa) |
| | Rv3399 | Possible S-adenosylmethionine-dependent methyltransferase; Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. (348 aa) |
| | cmaA1 | Cyclopropane mycolic acid synthase 1; Catalyzes the conversion of a double bond to a cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence. (287 aa) |
| | spoU | Probable tRNA/rRNA methylase SpoU (tRNA/rRNA methyltransferase); Could methylate the ribose at the nucleotide 34 wobble position in tRNA; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily. (154 aa) |
| | Rv3342 | Rv3342, (MTV016.42), len: 243 aa. Possible methyltransferase, similar to various proteins e.g. Q9I5X8|PA0558 hypothetical protein from Pseudomonas aeruginosa (255 aa), FASTA scores: opt: 496, E(): 4.4e-24,(39.85% identity in 236 aa overlap); Q9XBC9|CZA382.22c putative rRNA methylase from Amycolatopsis orientalis (259 aa), FASTA scores: opt: 473, E(): 1.2e-22, (42.45% identity in 245 aa overlap); Q9UTA8|SPAC25B8.10 putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (256 aa), FASTA scores: opt: 470, E(): 1.9e-22,(35.7% identity in 238 aa overlap); and Q9UTA9|SPAC25 [...] (243 aa) |
| | Rv3322c | Possible methyltransferase; Rv3322c, (MTV016.22c), len: 204 aa. Conserved hypothetical protein, showing weak similarity to proteins including several methyltransferases e.g. Q9X9V1|ORF8 putative methyltransferase from Streptomyces coelicolor (208 aa), FASTA scores: opt: 193, E(): 1e-05, (36.35% identity in 132 aa overlap); and Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa),FASTA scores: opt: 161, E(): 0.0014, (32.05% identity in 131 aa overlap); P74712|SLR1183 hypothetical 21.3 KDA protein from Synechocystis sp. strain PCC 6803 (194 aa),FASTA scores: [...] (204 aa) |
| | Rv3263 | Rv3263, (MTCY71.03), len: 553 aa. Probable DNA methylase, equivalent to Q9CCK4|ML0756 probable DNA methylase from Mycobacterium leprae (555 aa), FASTA scores: opt: 2980, E(): 2.1e-184, (81.9% identity in 541 aa overlap). Also similar to others e.g. P25240|MT57_ECOLI|ECO57IM modification methylase from Escherichia coli (544 aa), FASTA scores: opt: 595, E(): 1e-30, (30.35% identity in 507 aa overlap); P25201|MTA1_ACICA|ACCIM modification methylase ACCI from Acinetobacter calcoaceticus (540 aa), FASTA scores: opt: 366, E(): 5.7e-16, (23.35% identity in 467 aa overlap); Q56752|M-ACCI ACCI [...] (553 aa) |
| | mddA | Probable conserved integral membrane protein; Catalyzes the methylation of methanethiol (MeSH) to yield dimethylsulphide (DMS). (244 aa) |
| | Rv3204 | Possible DNA-methyltransferase (modification methylase); Rv3204, (MTCY07D11.22c), len: 101 aa. Possible DNA methyltransferase, similar to many hypothetical bacteriel proteins and methyltransferases e.g. Q9KT40|VC1065 methylated-DNA--protein-cysteine methyltransferase-related protein from Vibrio cholerae (100 aa), FASTA scores: opt: 170, E(): 2.8e-05, (34.35% identity in 99 aa overlap); Q9UTN9|SPAC1250.04c putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (108 aa), FASTA scores: opt: 161, E(): 0.00013, (36.65% identity in 101 aa overlap); Q9YDF4|APE0959 175 AA lo [...] (101 aa) |
| | Rv3120 | Rv3120, (MTCY164.30), len: 200 aa. Conserved hypothetical protein, with weak similarity to several hypothetical proteins and many N-methyl transferases e.g. Q9X9V1|ORF8 putative methyltransferase from Streptomyces coelicolor A3(2) (208 aa), FASTA scores: opt: 177, E(): 0.00011, (34.6% identity in 130 aa overlap); Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa), FASTA scores: opt: 147,E(): 0.011, (31.3% identity in 166 aa overlap); BAB52127|MLL5735 probable methyltransferase from Rhizobium loti (Mesorhizobium loti) (247 aa), FASTA scores: opt: 133,E(): [...] (200 aa) |
| | Rv3037c | Rv3037c, (MTV012.52c), len: 358 aa. Conserved hypothetical protein, similar in part to others e.g. O86799|SC6G4.36c from Streptomyces coelicolor (426 aa),FASTA scores: opt: 545, E(): 5.5e-27, (36.15% identity in 354 aa overlap); Q9UZW6|PAB0687 from Pyrococcus abyssi (386 aa), FASTA scores: opt: 262, E(): 3.5e-09, (31.0% identity in 200 aa overlap); Belongs to the methyltransferase superfamily. (358 aa) |
| | Rv3030 | Conserved protein; Probable S-adenosylmethionine-dependent methyltransferase required for the 6-O-methylation of the polysaccharide backbone of 6-O- methylglucosyl lipopolysaccharides (MGLP). (274 aa) |
| | Rv2966c | Rv2966c, (MTCY349.21), len: 188 aa. Possible methyltransferase, equivalent (but shorter 36 aa) to O69465|MLCB1243.09 hypothetical 23.0 KDA protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 872, E(): 9.1e-50, (74.2% identity in 182 aa overlap). Also similar to others e.g. Q9ZBR2|SC7A1.11 putative methylase from Streptomyces coelicolor (195 aa), FASTA scores: opt: 510,E(): 3.7e-26, (47.5% identity in 179 aa overlap); Q9F842 hypothetical methyltransferase (fragment) from Mycobacterium smegmatis (80 aa), FASTA scores: opt: 386,E(): 2.5e-18, (75.0% identity in 80 aa overlap); P [...] (188 aa) |
| | Rv2959c | Possible methyltransferase (methylase); Catalyzes the O-methylation of the hydroxyl group located on C-2 of the first rhamnosyl residue linked to the phenolic group of glycosylated phenolphthiocerol dimycocerosates (PGL) and p- hydroxybenzoic acid derivatives (p-HBAD). (245 aa) |
| | Rv2952 | Possible methyltransferase (methylase); Catalyzes the methylation of the lipid moiety of the intermediate compounds phthiotriol and glycosylated phenolphthiotriol dimycoserosates to form phthiocerol dimycocerosates (DIM A) and glycosylated phenolphthiocerol dimycocerosates (PGL). Belongs to the methyltransferase superfamily. Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase family. (270 aa) |
| | trmD | tRNA (guanine-N(1)-)-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. (230 aa) |
| | Rv2880c | Rv2880c, (MTCY274.11c), len: 275 aa. Conserved hypothetical protein, highly similar in N-terminus to others e.g. O86754|SC6A9.22c hypothetical 40.4 KDA protein from Streptomyces coelicolor (368 aa), FASTA scores: opt: 663, E(): 2.6e-33, (52.6% identity in 213 aa overlap); Q55880|Y098_SYNY3|SLL0098 hypothetical 38.9 KDA protein from Synechocystis sp. strain PCC 6803 (350 aa), FASTA scores: opt: 362, E(): 7.3e-15, (38.9% identity in 162 aa overlap); O66732|AQ_416 hypothetical 40.2 KDA protein from Aquifex aeolicus (348 aa), FASTA scores: opt: 321, E(): 2.4e-12, (39.75% identity in 146 aa [...] (275 aa) |
| | rlmN | Conserved hypothetical protein; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; Belongs to the radical SAM superfamily. RlmN family. (189 aa) |
| | cysG | Rv2847c, (MTCY24A1.10), len: 405 aa. Possible cysG,multifunctional enzyme, siroheme synthase containing uroporphyrin-III c-methyltransferase, precorrin-2 oxidase and ferrochelatase. C-terminus highly similar to many uroporphyrin-III c-methyltransferases e.g. Q51720|COBA uroporphyrinogen III methyltransferase from Propionibacterium freudenreichii (257 aa), FASTA scores: opt: 776, E(): 1.5e-39, (48.95% identity in 243 aa overlap); Q9HMY4|UROM|VNG2331G S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Halobacterium sp. strain NRC-1 (246 aa), FASTA scores: opt: 704, E(): [...] (405 aa) |
| | thyA | Probable thymidylate synthase ThyA (ts) (TSASE); Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. (263 aa) |
