STRINGSTRING
Rv2177c Rv2177c Rv0052 Rv0052 celA1 celA1 PE_PGRS1 PE_PGRS1 ldtA ldtA pepA pepA fbpC fbpC Rv0157A Rv0157A PE3 PE3 PE4 PE4 Rv0178 Rv0178 Rv0192 Rv0192 Rv0200 Rv0200 Rv0227c Rv0227c vapC51 vapC51 vapC24 vapC24 vapC25 vapC25 PE_PGRS3 PE_PGRS3 PE_PGRS4 PE_PGRS4 esxG esxG vapC2 vapC2 PPE5 PPE5 PPE6 PPE6 Rv0312 Rv0312 Rv0320 Rv0320 dnaK dnaK PPE7 PPE7 PPE8 PPE8 Rv0361 Rv0361 fba fba lpqK lpqK mmpS1 mmpS1 lpqL lpqL Rv0433 Rv0433 mazF1 mazF1 lpdC lpdC icl1 icl1 fadB2 fadB2 lprQ lprQ Rv0518 Rv0518 Rv0519c Rv0519c PE_PGRS7 PE_PGRS7 vapC4 vapC4 vapC27 vapC27 Rv0614 Rv0614 vapC29 vapC29 Rv0625c Rv0625c vapC5 vapC5 vapC6 vapC6 Rv0658c Rv0658c vapC7 vapC7 vapC8 vapC8 lpqP lpqP tuf tuf Rv0739 Rv0739 PE_PGRS8 PE_PGRS8 PE_PGRS9 PE_PGRS9 PE_PGRS10 PE_PGRS10 vapC31 vapC31 Rv0774c Rv0774c Rv0797 Rv0797 Rv0804 Rv0804 PE_PGRS13 PE_PGRS13 lpqQ lpqQ pdc pdc rpfA rpfA Rv0883c Rv0883c echA6 echA6 PPE14 PPE14 PE_PGRS16 PE_PGRS16 pepD pepD rpfB rpfB lpqU lpqU Rv1041c Rv1041c Rv1042c Rv1042c Rv1084 Rv1084 celA2b celA2b PE_PGRS22 PE_PGRS22 Rv1096 Rv1096 Rv1104 Rv1104 Rv1105 Rv1105 vapC32 vapC32 metE metE Rv1140 Rv1140 echA11 echA11 echA10 echA10 Rv1149 Rv1149 Rv1150 Rv1150 PE14 PE14 vapC33 vapC33 Rv1251c Rv1251c Rv1288 Rv1288 vapC10 vapC10 lprG lprG Rv1419 Rv1419 PE16 PE16 PE_PGRS27 PE_PGRS27 trxA trxA trxB1 trxB1 echA12 echA12 Rv1481 Rv1481 Rv1491c Rv1491c lprI lprI vapC11 vapC11 treX treX Rv1592c Rv1592c Rv1639c Rv1639c dsbF dsbF Rv1691 Rv1691 fadB3 fadB3 plcD plcD cut1 cut1 wag22 wag22 PE_PGRS31 PE_PGRS31 Rv1770 Rv1770 PPE31 PPE31 Rv1836c Rv1836c vapC13 vapC13 apa apa Rv1863c Rv1863c rpfC rpfC Rv1885c Rv1885c fbpB fbpB Rv1906c Rv1906c aceAa aceAa aceAb aceAb PPE34 PPE34 mpt63 mpt63 Rv1930c Rv1930c Rv1931c Rv1931c Rv1949c Rv1949c vapC14 vapC14 vapC35 vapC35 Rv1975 Rv1975 mpt64 mpt64 Rv1987 Rv1987 mazF6 mazF6 vapC15 vapC15 Rv2013 Rv2013 Rv2014 Rv2014 Rv2033c Rv2033c lipT lipT Rv2054 Rv2054 mazF7 mazF7 blaC blaC Rv2077c Rv2077c Rv2082 Rv2082 Rv2083 Rv2083 vapC37 vapC37 prcB prcB Rv2141c Rv2141c murC murC PE_PGRS38 PE_PGRS38 Rv2190c Rv2190c glnA1 glnA1 vapC16 vapC16 ptpA ptpA Rv2264c Rv2264c cut2 cut2 Rv2306A Rv2306A Rv2306B Rv2306B plcC plcC plcB plcB plcA plcA glyS glyS cfp2 cfp2 rpfD rpfD PE_PGRS41 PE_PGRS41 eis eis Rv2422 Rv2422 Rv2424c Rv2424c rpfE rpfE echA14 echA14 vapC38 vapC38 Rv2514c Rv2514c ldtB ldtB PE26 PE26 vapC17 vapC17 vapC39 vapC39 vapC18 vapC18 vapC19 vapC19 vapC40 vapC40 vapC41 vapC41 chiZ chiZ Rv2721c Rv2721c vapC21 vapC21 Rv2765 Rv2765 ald ald mazF9 mazF9 vapC22 vapC22 echA16 echA16 infB infB cysG cysG Rv2850c Rv2850c vapC23 vapC23 vapC43 vapC43 mpt83 mpt83 mpt70 mpt70 mpt53 mpt53 lppW lppW Rv2972c Rv2972c cfp6 cfp6 Rv3096 Rv3096 Rv3103c Rv3103c devR devR PPE50 PPE50 PPE51 PPE51 vapC49 vapC49 sahH sahH lpqC lpqC sapM sapM vapC44 vapC44 Rv3327 Rv3327 PPE54 PPE54 PPE55 PPE55 Rv3348 Rv3348 PPE56 PPE56 echA18 echA18 echA18.1 echA18.1 vapC47 vapC47 Rv3438 Rv3438 esxU esxU Rv3446c Rv3446c cut3 cut3 cut4 cut4 Rv3483c Rv3483c PE_PGRS54 PE_PGRS54 PE_PGRS56 PE_PGRS56 lpqF lpqF Rv3604c Rv3604c espA espA PPE65 PPE65 Rv3630 Rv3630 Rv3668c Rv3668c vapC48 vapC48 cut5a cut5a cut5b cut5b lpqH lpqH Rv3766 Rv3766 fbpD fbpD fbpA fbpA pirG pirG Rv3844 Rv3844 Rv3845 Rv3845 sodA sodA espR espR espE espE esxB esxB espJ espJ espB espB Rv3897c Rv3897c Rv3898c Rv3898c Rv3899c Rv3899c
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query proteins and first shell of interactors
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second shell of interactors
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proteins of unknown 3D structure
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a 3D structure is known or predicted
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Known Interactions
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gene neighborhood
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Rv2177cPossible transposase; Rv2177c, (MTV021.10c), len: 221 aa. Possible IS1558 transposase (see citation below), similar to several is element proteins and transposases but nearly identical to last 221 residues of MTCY428_23 (333 aa). FASTA scores: Z81451|MTCY428_23 Mycobacterium tuberculosis cosmid (333 aa) opt: 1491, E() : 0; 98.6% identity in 221 aa overlap. (221 aa)
Rv0052Conserved protein; Rv0052, (MTCY21D4.15), len: 187 aa. Conserved protein, similar to others including Rv1930c from Mycobacterium tuberculosis (174 aa). May be a membrane protein. (187 aa)
celA1Glucanase; Rv0062, (MTV030.05), len: 380 aa. Possible celA1,cellulase, similar to many. Seems to belong to cellulase family B (family 6 of glycosyl hydrolases). Note that previously known as celA. (380 aa)
PE_PGRS1PE-PGRS family protein PE_PGRS1; Rv0109, (MTV031.03c), len: 496 aa. PE_PGRS1, Member of the M. tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Q50615|Y0DP_MYCTU hypothetical glycine-rich 40.8 kDa protein from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1772, E(): 0,(57.3% identity in 513 aa overlap); etc. (496 aa)
ldtAProbable L,D-transpeptidase LdtA; Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. Is thought to play a role in adaptation to the nonreplicative state of M.tuberculosis. (251 aa)
pepARv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa),FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa protein precursor from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. Belongs to the s [...] (355 aa)
fbpCDiacylglycerol acyltransferase/mycolyltransferase Ag85C; The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria to fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha- trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehal [...] (340 aa)
Rv0157ARv0157A, len: 42 aa. Conserved protein, showing similarity to C-terminal part (aa 186-220) of O53976|Rv1975|MTV051.13 conserved hypothetical protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 173, E(): 3e-06, (62.5% identity in 40 aa overlap). (42 aa)
PE3PE family protein PE3; Plays significant roles in mycobacterial persistence during infection and modulates host immune response. (468 aa)
PE4Rv0160c, (MTV032.03c), len: 502 aa. PE4, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. Z92770|MTCI5_26c from Mycobacterium tuberculosis (525 aa),FASTA scores: opt: 816, E(): 0, (41.4% identity in 367 aa overlap); C-terminal region of O06801|RV1768|MTCY28.34 from Mycobacterium tuberculosis (618 aa), FASTA scores: opt: 417, E(): 6.7e-18, (53.5% identity in 142 aa overlap). Also highly similar to downstream ORF MTV032_2. (502 aa)
Rv0178Rv0178, (MTCI28.18), len: 244 aa. Probable conserved Mce-associated membrane protein, highly similar in C-terminus to CAC32130.1|AL583926 putative secreted protein from Mycobacterium leprae (184 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv1973, etc. Note that there is a 10 aa overlap with the upstream ORF. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (244 aa)
Rv0192Conserved hypothetical protein; Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. (366 aa)
Rv0200Rv0200, (MTV033.08), len: 229 aa. Possible conserved transmembrane protein, equivalent to Z95398|MLCL622.14 from Mycobacterium leprae (229 aa), FASTA scores: opt: 1147,E(): 0, (74.7% identity in 229 aa overlap). Also some similarity to Rv1973 from Mycobacterium tuberculosis (160 aa); and Rv1362c|Z75555|MTCY02B10_26 (220 aa), FASTA scores: opt: 134, E(): 0.063, (25.8% identity in 159 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (229 aa)
Rv0227cRv0227c, (MTCY08D5.22c), len: 421 aa. Possible conserved membrane protein, equivalent to AL022486|MLCB1883_4 from Mycobacterium leprae (448 aa),FASTA scores: opt: 2148, E(): 0, (76.6% identity in 423 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (421 aa)
vapC51Possible conserved membrane protein with PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB51; Belongs to the PINc/VapC protein family. (226 aa)
vapC24Possible toxin VapC24. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB24 (By similarity). Belongs to the PINc/VapC protein family. (145 aa)
vapC25Possible toxin VapC25. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB25.; Belongs to the PINc/VapC protein family. (142 aa)
PE_PGRS3PE-PGRS family protein PE_PGRS3; Rv0278c, (MTV035.06c), len: 957 aa. PE_PGRS3, Member of the Mycobacterium tuberculosis PE family (see citation below), PGRS subfamily of gly-rich proteins, similar to many e.g. Z95890|MTCY28_25|Rv1759c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 3849, E(): 0,(67.8% identity in 903 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1. (957 aa)
PE_PGRS4PE-PGRS family protein PE_PGRS4; Rv0279c, (MTV035.07c), len: 837 aa. PE_PGRS4, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002),similar to many e.g. Z95890|MTCY28_25|Rv0278c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 2677, E(): 0, (64.5% identity in 926 aa overlap). (837 aa)
esxGESAT-6 like protein EsxG (conserved protein TB9.8); EsxG, in complex with EsxH, disrupts ESCRT function and impairs host phagosome maturation, thereby promoting intracellular bacterial growth. The complex acts by interacting, via EsxH, with the host hepatocyte growth factor-regulated tyrosine kinase substrate (HGS/HRS), a component of the ESCRT machinery. EsxG stabilizes EsxH in the host cytosol. (97 aa)
vapC2Possible toxin VapC2; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an RNase. Upon expression in M.smegmatis inhibits translation, growth and colony formation. All its toxic effects are neutralized by coexpression with cognate antitoxin VapB2. (141 aa)
PPE5Rv0304c, (MTCY63.9c), len: 2204 aa. PPE5, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR),similar to others e.g. Z95324|MTY13E10_16 from Mycobacterium tuberculosis (1443 aa), FASTA scores: E(): 0,(50.6% identity in 1403 aa overlap); Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 989, E(): 0, (42.3% identity in 522 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (2204 aa)
PPE6Rv0305c, (MTCY63.10c), len: 963 aa. PPE6, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR),similar to others e.g. Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 1340, E(): 0,(40.9% identity in 815 aa overlap). (963 aa)
Rv0312Rv0312, (MTCY63.17), len: 620 aa. Conserved hypothetical protein with highly Pro-, Thr-rich C-terminus. Similar to Pro-,Thr-rich region in Rv2264c|AL021925|MTV022_14 from Mycobacterium tuberculosis (592 aa), FASTA scores: opt: 1075, E(): 0, (38.9% identity in 627 aa overlap). Also some similarity with Rv0350|dnaK from Mycobacterium tuberculosis. Possibly membrane protein; has hydrophobic stetch in its middle part. (620 aa)
Rv0320Rv0320, (MTCY63.25), len: 220 aa. Possible conserved exported protein, similar to some hypothetical proteins and to the middle part of a peptidase: NP_066789.1|10657900|AAG21739.1|AF116907 putative peptidase from Rhodococcus equi (546 aa). Also similar to Rv1728c|MTCY04C12.13c from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 497, E(): 1.2e-26, (41.8% identity in 225 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). (220 aa)
dnaKProbable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70); Acts as a chaperone; Belongs to the heat shock protein 70 family. (625 aa)
PPE7Rv0354c, (MTCY13E10.14c), len: 141 aa. PPE7, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. MTCY63_9 from Mycobacterium tuberculosis (2411 aa), FASTA scores: E(): 3.6e-11, (47.6% identity in 103 aa overlap). Possible continuation of ORF upstream, but no sequence error apparent. (141 aa)
PPE8PPE family protein PPE8; Rv0355c, (MTCY13E10.15c, MTCY13E10.16c,MTCY13E10.17c), len: 3300 aa. PPE8, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL009198|MTV004_5 from Mycobacterium tuberculosis (3716 aa), FASTA scores: opt: 2906, E(): 0, (40.9% identity in 3833 aa overlap); MTV004_3 FASTA scores: (39.0% identity in 3531 aa overlap); etc. Gene contains large number of clustered Major Polymorphic Tandem Repeats (MPTR). Related to MTCY13E10.16c, E(): 0; MTCY13E10.17c, E(): 0; MTCY48.17,E(): 0; MTCY98.0034c, E(): 0; MTCY03C7.23 E(): 0; MTCY98.0031c, E(): 0; [...] (3300 aa)
Rv0361Rv0361, (MTCY13E10.23), len: 275 aa. Probable conserved membrane protein (has hydrophobic stretch from residues 132-156), equivalent to AL023514|MLCB4_17|AA18949.1|AL023514 putative membrane protein from Mycobacterium leprae (292 aa), FASTA scores: opt: 1044, E(): 0, (58.6% identity in 292 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (275 aa)
fbaProbable fructose-bisphosphate aldolase Fba; Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. (344 aa)
lpqKRv0399c, (MTCY04D9.11c), len: 409 aa. Possible lpqK,conserved lipoprotein, showing some similarity to penicillin binding proteins and various peptidases e.g. DAC_STRSQ|P15555 d-alanyl-d-alanine carboxypeptidase protein (406 aa), FASTA scores: opt: 348, E(): 5.6e-16,(29.2% identity in 301 aa overlap). Also similar to other Mycobacterium tuberculosis PBPs and esterases. Has possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). (409 aa)
mmpS1Rv0403c, (MTCY04D9.16c), len: 142 aa. Probable mmpS1, conserved membrane protein (see citation below),highly similar to other Mycobacterial proteins e.g. YV33_MYCLE|P54880 hypothetical 16.9 kDa protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 458, E(): 1.6e-26, (46.9% identity in 143 aa overlap); YV33_MYCTU|Q11170 hypothetical 15.9 kDa protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 362, E(): 1.1e-19, (42.1% identity in 140 aa overlap); etc. Also similar to other MmpS proteins from Mycobacterium tuberculosis e.g. Rv0677c, Rv0451c, etc. Belongs to the [...] (142 aa)
lpqLProbable lipoprotein aminopeptidase LpqL; An aminopeptidase; acts on free N-terminal amino groups with a very strong preference for Leu in the first position. Belongs to the peptidase M28 family. M28A subfamily. (500 aa)
Rv0433Conserved hypothetical protein; ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity; Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily. (376 aa)
mazF1Possible toxin MazF1; Toxic component of a type II toxin-antitoxin (TA) system, its cognate antitoxin is MazE1 (Probable). Probably an endoribonuclease (By similarity); Belongs to the PemK/MazF family. (93 aa)
lpdCDihydrolipoyl dehydrogenase; Lipoamide dehydrogenase is an essential component of the alpha-ketoacid dehydrogenase complexes, namely the pyruvate dehydrogenase (PDH) complex, the branched-chain alpha-ketoacid dehydrogenase (BCKADH) complex, and likely also the 2-oxoglutarate dehydrogenase (ODH) complex. Catalyzes the reoxidation of dihydrolipoyl groups which are covalently attached to the lipoate acyltransferase components (E2) of the complexes. Is also able to catalyze the transhydrogenation of NADH and thio-NAD(+) in the absence of D,L- lipoamide, and the NADH-dependent reduction of [...] (464 aa)
icl1Isocitrate lyase Icl (isocitrase) (isocitratase); Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It could also catalyze the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route) (By similarity). (428 aa)
fadB23-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD); Catalyzes the NAD-dependent oxidation of beta-hydroxybutyryl- CoA to acetoacetyl-CoA in vitro at pH 10. Also catalyzes the reverse reaction albeit in a lower pH range of 5.5-6.5. The reverse reaction is able to use NADPH as well as NADH; Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. (286 aa)
lprQProbable conserved lipoprotein LprQ; Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. (451 aa)
Rv0518Possible exported protein; GDSL lipase that catalyzes the hydrolysis of p-nitrophenyl (pNP) esters. pNP-decanoate (C10) is the preferred substrate. It can also use pNP-octanoate (C8), pNP-dodecanoate (C12) and pNP- tetradecanoate (C14). Has lower activity with pNP-butyrate (C4), pNP- palmitate (C16) and pNP-stearate (C18). Does not show phospholipase A1 activity. Might help bacteria to utilize available lipids for its growth as well as provide resistance to various intracellular stresses by cell wall modulation resulting in enhanced intracellular survival. (231 aa)
Rv0519cRv0519c, (MTCY20G10.09c), len: 300 aa. Possible conserved membrane protein, with hydrophobic region near N-terminus. Could be a lipase. Similar to Rv0774c|MTCY369.19c|A70708 from Mycobacterium tuberculosis (312 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00120 Lipases, serine active site. (300 aa)
PE_PGRS7PE-PGRS family protein PE_PGRS7; Rv0578c, (MTV039.16c), len: 1306 aa. PE_PGRS7,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many other PGRS proteins e.g. MTCY493.04|Z95844 from Mycobacterium tuberculosis (1329 aa), FASTA scores: opt: 3994, E(): 0, (54.6% identity in 1375 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures possibly fortuitously. (1306 aa)
vapC4Possible toxin VapC4; Toxic component of a type II toxin-antitoxin (TA) system. Probably exerts its toxic effect by binding to mRNA, inhibiting translation. Binds to, recognizes and cleaves ssRNA at ACGC and AC(A/U)GC sequences, usually between the G and C; cleavage is not very efficient, nor is cleavage required to inhibit protein synthesis. Upon expression in situ, in M.smegmatis or E.coli inhibits cell growth and colony formation; in at least E.coli also causes increased levels of cellular RNA. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB4. (130 aa)
vapC27Possible toxin VapC27. Contains PIN domain; Probably the toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Its cognate antitoxin is VapB27. Belongs to the PINc/VapC protein family. (137 aa)
Rv0614Rv0614, (MTCY19H5.07c), len: 330 aa. Conserved hypothetical protein, similar in part to Mycobacterium tuberculosis hypothetical proteins e.g. YY16_MYCTU|Q10685|Rv2077c|MT2137|MTCY49.16c conserved hypothetical protein (323 aa), FASTA scores: opt: 200, E(): 0.00016, (28.3% identity in 269 aa overlap); MTCY9F9_15 FASTA score: (40.3% identity in 144 aa overlap), Rv1949c,Rv2542, etc. Several start sites are possible; first start has been chosen. Note that this ORF overlaps with the upstream ORF. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT- [...] (330 aa)
vapC29Possible toxin VapC29. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. Its cognate antitoxin is VapB29 (By similarity). Has ribonuclease activity. (133 aa)
Rv0625cRv0625c, (MTCY20H10.06c), len: 246 aa. Probable conserved transmembrane protein, showing similarity with others e.g. CAB61866.1|AL133252 putative integral membrane protein from Streptomyces coelicolor (249 aa). Also similar to Rv1491c|MTCY277_13 from Mycobacterium tuberculosis. Contains potential membrane spanning regions. (246 aa)
vapC5Possible toxin VapC5; Probable toxic component of a type II toxin-antitoxin (TA) system. The cognate antitoxin is VapB5. Has limited RNase activity on substrates; activity is seen with a VapC5-VapB5 complex. (135 aa)
vapC6Possible toxin VapC6; Toxic component of a type II type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB6 (By similarity). Belongs to the PINc/VapC protein family. (127 aa)
Rv0658cRv0658c, (MTCI376.18), len: 238 aa. Probable conserved integral membrane protein, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and showing some similarity with P33774|YPRB_ECOLI hypothetical 24.3 kDa protein from Escherichia coli (217 aa), FASTA scores: opt: 174, E(): 5.3e-05, (25.6% identity in 223 aa overlap). Also similar to Rv1863c and Rv0804 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (238 aa)
vapC7Possible toxin VapC7; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB7 (By similarity). Belongs to the PINc/VapC protein family. (145 aa)
vapC8Possible toxin VapC8; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB8 (By similarity); Belongs to the PINc/VapC protein family. (112 aa)
lpqPRv0671, (MTCI376.03c), len: 280 aa. Possible lpqP,conserved lipoprotein, similar to U00012|B1308_F2_43|Q49658 from Mycobacterium leprae (302 aa), FASTA scores: opt: 449,E(): 2.4e-22, (37.6% identity in 242 aa overlap). Also highly similar to lpqC|Rv3298c|MTCY71.38c putative lipoprotein from Mycobacterium tuberculosis (304 aa). Also similar to a large variety of proteins including various esterases and poly(3-hydroxyalkanoate) depolymerases, e.g. NP_249234.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (322 aa); C-terminus of AAD45376.1|AF164516_1|AF164516 cinnamoyl ester [...] (280 aa)
tufProbable iron-regulated elongation factor TU Tuf (EF-TU); This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (396 aa)
Rv0739Rv0739, (MTV041.13), len: 268 aa. Conserved hypothetical protein, showing some similarity to Mycobacterium tuberculosis proteins Rv0026 (448 aa), FASTA score: (37.6% identity in 101 aa overlap)and Rv0025 (120 aa), FASTA score: (32.4% identity in 142 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (268 aa)
PE_PGRS8PE-PGRS family protein PE_PGRS8; Rv0742, (MTV041.16), len: 175 aa. PE_PGRS8, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to many Mycobacterium tuberculosis PGRS-type proteins e.g. Z78020|MTCY1A11_25 (498 aa), FASTA scores: opt: 766, E(): 6.1e-25, (73.6% identity in 178 aa overlap). Similarity suggests ORF starts with ATA start codon. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (175 aa)
PE_PGRS9PE-PGRS family protein PE_PGRS9; Rv0746, (MTV041.20), len: 783 aa. PE_PGRS9, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2429, E(): 0,(56.9% identity in 873 aa overlap). Also similar to other PE-PGRS family proteins e.g. AL0212|MTV008_46 FASTA score: (48.8% identity in 887 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (783 aa)
PE_PGRS10PE-PGRS family protein PE_PGRS10; Rv0747, (MTV041.21), len: 801 aa. PE_PGRS10, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2772, E(): 0,(60.9% identity in 941 aa overlap). Also similar to other PE-PGRS family proteins e.g. Z95844|MTCY493_2 FASTA score: (50.2% identity in 815 aa overlap). Contains PS00012 Phosphopantetheine attachment site. This region is a possible MT-complex-specific genomic island (See Be [...] (801 aa)
vapC31Possible toxin VapC31. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB31 (By similarity). (142 aa)
Rv0774cRv0774c, (MTCY369.19c), len: 303 aa. Possible conserved exported protein with hydrophobic region near N-terminus, highly similar, except in N-terminus, to Rv0519c|Z97831|MTY20G10.09c|O33364 hypothetical protein from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature, and PS00120 Lipases, serine active site. So could be a lipase. Start changed since first submission (-9 aa). Predicted to be an outer membrane protein (See Song et al., 2008). (303 aa)
Rv0797Rv0797, (MTCI249B.03c, MTV042.07), len: 364 aa. Putative transposase for IS1547; almost identical to (but 20 aa shorter than) Y13470|MTY13470_2 from Mycobacterium tuberculosis (383 aa). Also similar to other transposases e.g. MAIS1110A _1|Q48909 transposase from Mycobacterium avium (464 aa), FASTA scores: opt: 226, E(): 2.4e-08,(30.7% identity in 199 aa overlap). Also slight similarity to Rv2014|MTCY39.03c from Mycobacterium tuberculosis (222 aa), FASTA score: (24.8% identity in 141 aa overlap). (364 aa)
Rv0804Rv0804, (MTCY07H7A.05c), len: 209 aa. Conserved hypothetical protein, showing similarity with C-terminus of Rv1863c|MTCY359.10 conserved hypothetical protein from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 199, E(): 1.2e-05, (33.2% identity in 220 aa overlap); and Rv0658c. Contains PS01151 Fimbrial biogenesis outer membrane usher protein signature. (209 aa)
PE_PGRS13PE-PGRS family protein PE_PGRS13; Rv0833, (MTV043.25), len: 749 aa. PE_PGRS13, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), but lacking N-terminal domain (present in preceding ORF),possibly due to frameshift. Similar in part to many others e.g. MTCY28_25|Z95890 (914 aa), FASTA scores: opt: 2726,E(): 0, (60.1% identity in 776 aa overlap); etc. (749 aa)
lpqQRv0835, (MTV043.27), len: 214 aa. Possible lpqQ,lipoprotein. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. (214 aa)
pdcProbable pyruvate or indole-3-pyruvate decarboxylase Pdc; Decarboxylates branched-chain and aromatic alpha-keto acids to aldehydes; Belongs to the TPP enzyme family. (560 aa)
rpfAPossible resuscitation-promoting factor RpfA; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Stimulates growth of stationary phase M.bovis (a slow-growing Mycobacterium), reduces the lag phase of diluted fast-growers M.smegmatis and Micrococcus [...] (407 aa)
Rv0883cRv0883c, (MTCY31.11c), len: 253 aa. Conserved hypothetical protein, equivalent to O3306|MLCB57_16 conserved hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: E(): 0, (79.4% identity in 253 aa overlap). Also highly similar to N_terminus of AL009204|SC9B10_22 hypothetical protein from Streptomyces coelicolor (352 aa), FASTA scores: E(): 6.1e-20, (35.0% identity in 246 aa overlap). (253 aa)
echA6Possible enoyl-CoA hydratase EchA6 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Could possibly oxidize fatty acids using specific components. (243 aa)
PPE14Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. (423 aa)
PE_PGRS16PE-PGRS family protein PE_PGRS16; Rv0977, (MTV044.05), len: 923 aa. PE_PGRS16, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to other PGRS-type sequences e.g. AL0091|MTV004_1 from Mycobacterium tuberculosis (1125 aa), FASTA score: (45.4% identity in 959 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (51.5% identity in 750 aa overlap); etc. (923 aa)
pepDRv0983, (MTV044.11), len: 464 aa. Probable pepD (alternate gene name: mtb32b), secreted or membrane serine protease (see citation below), equivalent (but longer 18 aa in N-terminus) to AL035500|MLCL373_17|T45448 probable serine proteinase from Mycobacterium leprae (452 aa), FASTA score: (74.2% identity in 466 aa overlap); and highly similar to others from Mycobacterium leprae. Also highly similar (except in N-terminus) to other proteases e.g. CAC01350.1|AL390975 putative protease from Streptomyces coelicolor (542 aa); NP_440705.1|NC_000911|HtrA serine protease from Synechocystis sp. (4 [...] (464 aa)
rpfBProbable resuscitation-promoting factor RpfB; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity; Belongs to the transglycosylase family. Rpf subfamily. (362 aa)
lpqURv1022, (MTCY10G2.27c), len: 243 aa. Probable lpqU conserved lipoprotein. Similar to Mycobacterium tuberculosis hypothetical protein Rv1230c|MTV006.02C, FASTA scores: E(): 2.8e-18, (37.9% identity in 240 aa overlap). Similar to AL133423|SC4A7.37 hypothetical protein from Streptomyces coelicolor (421 aa), FASTA scores: opt: 474,E(): 2.7e-21, (42.2% identity in 211 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. (243 aa)
Rv1041cRv1041c, (MTCY10G2.08), len: 287 aa. Probable is like-2 transposase, overlaps MTCY10G2.07. Similar to Q00430|X53945 insertion element IS869 hypothetical protein from Agrobacterium tumefaciens (186 aa), FASTA scores: opt: 173, E(): 0.00016, (40.9% identity in 176 aa overlap). Similar to Rv1150, C-terminal part of transposase of putative Mycobacterium tuberculosis is like-1. MTCY10G2.07 and MTCY10G2.08 are frameshifted with respect to Mycobacterium tuberculosis Q50761 transposase, the 10G2 cosmid sequence appears to be correct. This region is a possible MT-complex-specific genomic island [...] (287 aa)
Rv1042cRv1042c, (MTCY10G2.07), len: 135 aa. Probable is like-2 transposase, similar to Q50761 transposase from Mycobacterium tuberculosis (308 aa), FASTA scores: opt: 823, E(): 0, (99.1% identity in 117 aa overlap). Second copy is Rv1149. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007). (135 aa)
Rv1084Conserved protein; Rv1084, (MTV017.37), len: 673 aa. Conserved protein,similar to P37512|YYAL_BACSU hypothetical protein from Bacillus subtilis (689 aa), FASTA scores: opt: 1063, E() : 0, (36.5% identity in 696 aa overlap); AE0009|AE000983_10 Archaeoglobus fulgidus section 1 (642 aa), FASTA scores: opt: 1018, E(): 0, (37.2% identity in 600 aa overlap). Also similar to AE001938|AE001938_9 Deinococcus radiodurans (690 aa), FASTA scores: opt: 1097, E(): 0, (41.6% identity in 694 aa overlap). (673 aa)
celA2bRv1090, (MTV017.43), len: 151 aa. Probable celA2b,second part of cellulase (endoglucanase), similar to C-terminus of others e.g. O08468 cellulase CEL2 from Streptomyces halstedi (377 aa), FASTA scores: opt: 554,E(): 1.2e-30, (52.0% identity in 152 aa overlap); etc. Gene appears to have been inactivated by frameshift mutations but no errors could be found that would account for this. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007); Belongs to the glycosyl hydrolase 12 (cellulase H) family. (151 aa)
PE_PGRS22PE-PGRS family protein PE_PGRS22; Rv1091, (MTV017.44), len: 853 aa. PE_PGRS22, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to Rv1087|AL021897|MTV017_39 Mycobacterium tuberculosis H37Rv (767 aa), FASTA scores: opt: 2819, E(): 0, (60.0% identity in 860 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007). (853 aa)
Rv1096Rv1096, (MTV017.49), len: 291 aa. Possible glycosyl hydrolase, possibly deacetylase or esterase. Equivalent to AL049491|MLCB1222_13 Mycobacterium leprae (291 aa) (81.3% identity in 289 aa overlap). Similar at the C-terminus to enzymes involved in carbohydrate degradation including Z99110|BSUB0007_92 endo-1,4-beta-xylanase homolog yjeA from Bacillus subtilis (467 aa), FASTA scores: opt: 418, E(): 2.6e-17, (38.6% identity in 184 aa overlap); M64552|STMXLNB_2 acetyl-xylan esterase from Streptomyces lividans (335 aa), FASTA scores: opt: 371, E(): 1.1e-14,(31.6% identity in 237 aa overlap); [...] (291 aa)
Rv1104Rv1104, (MTV017.57), len: 229 aa. Possible para-nitrobenzyl esterase (fragment; possibly first part). Similar to the N-terminal domain of many e.g. P37967|PNBA_BACSU Bacillus subtilis (489 aa), FASTA scores: opt: 715, E(): 0, (53.4% identity in 191 aa overlap). Gene may be inactivated as a frameshift is required to obtain a product continuing in MTV017.58|Rv1105. (229 aa)
Rv1105Rv1105, (MTV017.58), len: 171 aa. Possible para-nitrobenzyl esterase (fragment; possibly second part). Similar to C-terminal domain of many e.g. P71048 para-nitrobenzyl esterase from Bacillus subtilis (489 aa),FASTA scores: opt: 248, E(): 2.7e-10, (32.3% identity in 167 aa overlap). Gene may be inactivated as a frameshift is required to obtain a product continuing from MTV017.57|Rv1104. Start changed since first submission. (171 aa)
vapC32Possible toxin VapC32. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB32.; Belongs to the PINc/VapC protein family. (124 aa)
metE5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. (759 aa)
Rv1140Rv1140, (MTCI65.07), len: 282 aa. Probable integral membrane protein. Weak similarity in C-terminus to hypothetical Escherichia coli proteins YPRA and YPRB,possibly membrane-bound e.g. YPRA_ECOLI hypothetical 24.3 kDa protein (URF 1) (217 aa), FASTA scores: opt: 166, E(): 0.00062, (31.0% identity in 158 aa overlap). (282 aa)
echA11Probable enoyl-CoA hydratase EchA11 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv1141c, (MTCI65.08c), len: 268 aa. Probable echA11,enoyl-CoA hydratase, similar to others e.g. P24162|ECHH_RHOCA probable enoyl-CoA hydratase from Rhodobacter capsulatus(257 aa); CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA scores: opt: 513, E():1e-25, (36.1% identity in 249 aa overlap); etc. Also similarity with naphthoate synthases. Also highly similar to downstream ORF Rv1142c|MTCI65.09|echA10 probable enoyl-CoA hydratase from Mycobacterium tuberculosis ( [...] (268 aa)
echA10Probable enoyl-CoA hydratase EchA10 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv1142c, (MTCI65.09c), len: 268 aa. Probable echA10,enoyl-CoA hydratase, similar to others e.g. CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA scores: opt: 525, E(): 1.3e-26, (35.1% identity in 251 aa overlap); NP_420658.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (267 aa); NP_438092.1|NC_003078 putative enoyl-CoA hydratase protein from Sinorhizobium meliloti (263 aa); etc. Also similarity with naphthoate synthases. Also [...] (268 aa)
Rv1149Possible transposase; Rv1149, (MTCI65.16), len: 135 aa. Possible transposase. Identical to 117 aa N-terminal region of S21394|X65618 transposase of Mycobacterium tuberculosis (308 aa), FASTA scores: opt: 823, E(): 0, (99.1% identity in 117 aa overlap). Second copy is Rv1042c|MTCY10G2.07. (135 aa)
Rv1150Possible transposase (fragment); Rv1150, (MTCI65.17), len: 183 aa. Possible fragment of transposase (pseudogene). Identical to C-terminal part of S21394 transposase of putative Mycobacterium tuberculosis is element (308 aa), FASTA scores: opt: 959,E(): 0, (99.3% identity in 145 aa overlap). The transposase described here may be made by a -1 frame shifting mechanism during translation that fuses Rv1149|MTCI65.16 and Rv1150|MTCI65.17. No evidence found to account for discrepancy with previously published sequence. Second copy is Rv1041c|MTCY10G2.08. (183 aa)
PE14Rv1214c, (MTCI364.26c), len: 110 aa. PE14, Member of Mycobacterium tuberculosis PE family (see citation below),appears to be frameshifted but sequence appears to be correct. The 5'-end is atypical as first 9 aa appear to be missing. (110 aa)
vapC33Possible toxin VapC33. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB33.; Belongs to the PINc/VapC protein family. (143 aa)
Rv1251cRv1251c, (MTV006.23c), len: 1139 aa. Conserved hypothetical protein, showing some similarity in C-terminal region with other proteins from eukaryotes and bacteria e.g. NP_142121.1 hypothetical protein from Pyrococcus horikoshii (1188 aa); and some similarity to GTP-binding proteins e.g. P23249|MV10_MOUSE putative GTP-binding protein (1004 aa), FASTA scores: opt: 228, E(): 1.7e-06,(27.7% identity in 560 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (1139 aa)
Rv1288Conserved protein; Exhibits lipolytic activity with medium chain length esters as optimum substrates. In vitro, pNP-caprylate (C8) is the optimum substrate followed by pNP-capricate (C10). May modulate the cell wall lipids to favor the survival of bacteria under stress conditions. (456 aa)
vapC10Possible toxin VapC10; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB10 (By similarity). Belongs to the PINc/VapC protein family. (133 aa)
lprGConserved lipoprotein LprG; Probably helps membrane protein Rv1410c (P55) transport triacylglycerides (TAG) across the inner cell membrane into the periplasm; TAG probably regulates lipid metabolism and growth regulation. Binds TAG and transfers it between lipid bilayers, probably to the outer membrane in vivo. Binds di- and triacylated phosphatidyl-myo-inositol mannosides (PIMs), and glycolipid lipoglycan modulins lipoarabinomannan (LAM) and lipomannan (LM), facilitating their recognition by TLR2. Binds LM > PIM6 > ManLAM > PI-LAM > PIM2 (mannose-capped LAM and phospho-myo-inositol-ca [...] (236 aa)
Rv1419Unknown protein; Rv1419, (MTCY21B4.37), len: 157 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al.,2008). (157 aa)
PE16PE family protein PE16; Esterase that hydrolyzes short to medium chain fatty acid esters with the highest specific activity for p-nitrophenyl caproate (pNPC6). Has lower activity with p-nitrophenyl caprylate (pNPC8) and p- nitrophenyl butyrate (pNPC4). Has weak activity with p-nitrophenyl caprate (pNPC10) and p-nitrophenyl laurate (pNPC12). Does not possess lipolytic activity and cutinase activity. (528 aa)
PE_PGRS27PE-PGRS family protein PE_PGRS27; Rv1450c, (MTCY493.04), len: 1329 aa. PE_PGRS27,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu,2002), similar to Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), fasta scores: opt: 2112, E(): 0, (56.5% identity in 630 aa overlap). (1329 aa)
trxARv1470, (MTV007.17), len: 124 aa. Probable trxA,thioredoxin, similar to many e.g. P12243|THI1_SYNP7 thioredoxin 1 from Synechococcus sp. (106 aa), FASTA scores: opt: 201, E(): 9.2e-08, (35.4% identity in 99 aa overlap); etc. Highly similar to downstream ORF Rv1471|trxB1 probable thioredoxin from Mycobacterium tuberculosis (123 aa), FASTA scores: opt: 402, E(): 0,(54.4% identity in 114 aa overlap). Warning: note that Rv3914|MT4033|MTV028.05|trxC can be alternatively named trxA. (124 aa)
trxB1Rv1471, (MTV007.18), len: 123 aa. Probable trxB1,thioredoxin, similar to many bacterial thioredoxins e.g. P33636|THI2_ECOLI from Escherichia coli (139 aa), FASTA scores: opt: 290, E(): 1.8e-13, (44.3% identity in 97 aa overlap); etc. Highly similar to Rv1470|TrxA probable thioredoxin from Mycobacterium tuberculosis (124 aa), FASTA scores: opt: 402, E(): 1.2e-32, (54.4% identity in 114 aa overlap). Contains PS00194 Thioredoxin family active site. Belongs to the thioredoxin family. Note that previously known as trxB. (123 aa)
echA12Possible enoyl-CoA hydratase EchA12 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Could possibly oxidize fatty acids using specific components. (285 aa)
Rv1481Probable membrane protein; Rv1481, (MTCY277.02), len: 335 aa. Probable membrane protein, highly similar to YS02_MYCAV|O07395 hypothetical 36.1 kDa protein mav335 from Mycobacterium avium (335 aa),FASTA scores: opt: 1904, E(): 0, (89.0% identity in 337 aa overlap). Similar to AF116251|AF116251_1 BatA protein from Bacteroides fragilis (327 aa), FASTA scores: opt: 317, E(): 2e-12, (26.5% identity in 340 aa overlap). (335 aa)
Rv1491cConserved membrane protein; Rv1491c, (MTCY277.13c), len: 252 aa. Conserved membrane protein. Similar to hypothetical proteins from many organisms e.g. YDJZ_ECOLI|P76221 Escherichia coli (235 aa), FASTA scores: opt: 223, E():6.7 e-07, (31.7% identity in 145 aa overlap); AL133252|SCE46.15 Streptomyces coelicolor (249 aa), FASTA scores: opt: 378, E(): 1.5e-17,(39.1% identity in 169 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical protein Rv0625c. (252 aa)
lprIPossible lipoprotein LprI; Strongly binds and inhibits lysozyme, may help bacteria survive in lysozyme-producing host cells. When overexpressed in M.tuberculosis or M.smegmatis increases resistance to hen egg white lysozyme. M.smegmatis overexpressing LprI survive better during intracellular infection of peritoneal and monocyte-derived macrophages, both of which produce lysozyme during infection; M.smegmatis does not encode this protein. Somewhat better survival is seen in human cell lines when M.smegmatis cells express both proteins from this operon, i.e. GlbN (HbN) and LprI; In the C [...] (197 aa)
vapC11Possible toxin VapC11; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an RNase. Upon expression in E.coli and M.smegmatis inhibits translation, cell growth and colony formation. Its toxic effects on cell growth and colony formation are neutralized by coexpression with cognate antitoxin VapB11; the effect on translation has not been tested but is probably also neutralized. (134 aa)
treXRv1564c, (MTCY48.01), len: 721 aa. Probable treX (previously called glgX), Maltooligosyltrehalose synthase. Strong similarity to D83245|g1890053 treX, glycogen debranching enzyme (glgX) from Sulfolobus acidocaldarius (713 aa), FASTA score: opt: 2396, E(): 0, (48.4% identity in 709 aa overlap); similar to GLGX_HAEIN|P45178 glycogen operon protein glgx (659 aa), FASTA scores: opt: 1512, E(): 0, (42.3% identity in 645 aa overlap); Belongs to the glycosyl hydrolase 13 family. (721 aa)
Rv1592cRv1592c, (MTCY336.12), len: 446 aa. Conserved hypothetical protein, some similarity to Q49629|B1170_F1_46 from Mycobacterium leprae (132 aa), FASTA results: opt: 332, E(): 4.5e-14, (56.3% identity in 87 aa overlap). Nearly identical to truncated Mycobacterium bovis BCG protein (148 aa) AF041819|AF041819_11; Belongs to the AB hydrolase superfamily. Lipase family. (446 aa)
Rv1639cRv1639c, (MTCY06H11.03c), len: 489 aa. Conserved hypothetical membrane protein. Some similarity to P35866|YLI2_CORGL Hypothetical 45.7 kDa protein from Corynebacterium glutamicum (426 aa), FASTA scores: opt: 511, E(): 2.4e-23, (28.9% identity in 370 aa overlap). Contains PS00904 protein phenyltransferases alpha subunit repeat signature. (489 aa)
dsbFRv1677, (MTV047.12), len: 182 aa. Probable dsbF,conserved lipoprotein possibly involved in thiol:disulfide interchange. Highly similar to C-terminus of Z74024|MTCY274.09 mpt53 soluble secreted antigen precursor from Mycobacterium tuberculosis (173 aa), FASTA scores: opt: 482, E(): 3.6e-23, (52.8% identity in 142 aa overlap). Also some similarity to P52237|TIPB_PSEFL thiol:disulfide interchange protein TIPB precursor from Pseudomonas fluorescens (178 aa), FASTA scores: opt: 190, E(): 4.4e-05,(28.5% identity in 151 aa overlap); and P33926|DSBE_ECOLI thiol:disulfide interchange protein fr [...] (182 aa)
Rv1691Rv1691, MTCI125.13, len: 250 aa. Conserved hypothetical protein, similar to Q9S210|SCI51.30C|AL109848 Hypothetical protein from Streptomyces coelicolor (210 aa),FASTA score: opt: 556, E(): 6.4e-27, (50.6% identity in 180 aa overlap). (250 aa)
fadB3Rv1715, (MTV048.02), len: 304 aa. Probable fadB3,3-hydroxybutyryl-CoA dehydrogenase, highly similar to many e.g. NP_107236.1|NC_002678 3-hydroxybutyryl-CoA dehydrogenase from Mesorhizobium loti (309 aa); NP_250319.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (509 aa); P45856|HBD_BACSU probable 3-hydroxybutyryl-CoA dehydrogenase from Bacillus subtilis (287 aa), FASTA scores: opt: 488, E(): 1.5e-24, (38.7% identity in 279 aa overlap); etc. Could belong to the 3-hydroxyacyl-CoA dehydrogenase family. (304 aa)
plcDRv1755c, (MT1799, MTCY28.21c), len: 280 aa. Probable plcD, phospholipase C 4 (fragment) (see citations below),highly similar to C-terminus of other phospholipases e.g. CQ50771|Rv2351c|PLCA|MTP40|MT2416|MTCY98.20c phospholipase C 1 from Mycobacterium tuberculosis (512 aa), FASTA score: (71.1% identity in 284 aa overlap); etc. Note that this ORF has been interrupted by insertion of IS6110 element. Belongs to the bacterial phospholipase C family. (280 aa)
cut1Probable cutinase Cut1; Rv1758, (MTCY28.24), len: 174 aa. Probable cut1,serine esterase, cutinase family, similar to Rv2301|CUT2_MYCTU|Q50664 probable cutinase cy339.08c precursor from Mycobacterium tuberculosis (219 aa), FASTA scores: opt: 369, E(): 1. 1e-16, (39.1% identity in 179 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical cutinases Rv3452, Rv1984c, Rv3451 and Rv3724. CDS has been interrupted by IS6110 insertion element and 5'-end deleted. Belongs to the cutinase family. (174 aa)
wag22PE-PGRS family protein Wag22; Rv1759c, (MT1807, MTCY28.25c), len: 914 aa. Wag22,antigen member (see citations below) of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, highly similar to others e.g. MT1367|Q10637 hypothetical glycine-rich 49.6 kDa protein from Mycobacterium tuberculosis (603 aa), FASTA scores: opt: 2010, E(): 0, (53.0% identity in 724 aa overlap); etc. (914 aa)
PE_PGRS31PE-PGRS family protein PE_PGRS31; Rv1768, (MTCY28.34), len: 618 aa. PE_PGRS31, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to Q50615 hypothetical 40.8 kDa protein (498 aa),FASTA scores: opt: 1703, E(): 0, (57.4% identity in 566 aa overlap). (618 aa)
Rv1770Conserved protein; Rv1770, (MTCY28.36), len: 428 aa. Conserved protein,highly similar in N-terminus to Q49882 Hypothetical protein from Mycobacterium leprae from cosmid L247 (83 aa), FASTA scores: opt: 301, E(): 1e-12, (56.5% identity in 85 aa overlap). (428 aa)
PPE31Rv1807, (MTV049.29), len: 399 aa. PPE31, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1789|MTV049.11|AL022021 (393 aa), FASTA scores: opt: 1169, E(): 0, (49.5% identity in 412 aa overlap). (399 aa)
Rv1836cConserved protein; Rv1836c, (MTCY1A11.07), len: 677 aa. Conserved protein. Equivalent to MLCB1788.28|AL008609 hypothetical protein from Mycobacterium leprae (710 aa), FASTA scores: opt: 2938, E(): 0, (66.0% identity in 714 aa overlap). Contains PS00036 bZIP transcription factors basic domain signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (677 aa)
vapC13Possible toxin VapC13; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB13 (By similarity). (131 aa)
apaAlanine and proline-rich secreted protein Apa; A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals. Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells. Functions as an adhesin, binds to mouse macrophages via mannose residues. Might interact via host CD209. Belongs to the Apa family. (325 aa)
Rv1863cRv1863c, (MTCY359.10), len: 256 aa. Probable conserved integral membrane protein, similar to Rv0804|Z95618|MTCY7H7A.05 Hypothetical protein from Mycobacterium tuberculosis (209 aa), FASTA scores: opt: 199, E(): 1e-06, (33.2% identity in 220 aa overlap); and Rv0658c. (256 aa)
rpfCProbable resuscitation-promoting factor RpfC; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Belongs to the transglycosylase family. Rpf subfamily. (176 aa)
Rv1885cChorismate mutase; Catalyzes the Claisen rearrangement of chorismate to prephenate. May play some role in the pathogenicity. (199 aa)
fbpBDiacylglycerol acyltransferase/mycolyltransferase Ag85B; The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha- trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-treha [...] (325 aa)
Rv1906cConserved protein; Rv1906c, (MTCY180.12), len: 156 aa. Conserved protein, possibly exported protein, equivalent to Mycobacterium leprae AJ000521|MLCOSL672.01 (153 aa), FASTA scores: opt: 637, E(): 2.6e-28, (63.2% identity in 155 aa overlap). Also similar to M. tuberculosis hypothetical exported protein, Rv1352. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004). Predicted to be an outer membrane protein (See Song et al., 2008). (156 aa)
aceAaProbable isocitrate lyase AceAa [first part] (isocitrase) (isocitratase) (Icl); Together with AceAb, they could catalyze the formation of succinate and glyoxylate from isocitrate. (367 aa)
aceAbProbable isocitrate lyase AceAb [second part] (isocitrase) (isocitratase) (Icl); Together with AceAa, they could catalyze the formation of succinate and glyoxylate from isocitrate. (398 aa)
PPE34PPE family protein PPE34; Facilitates a shift in the ensuing immunity toward the Th2 phenotype and could aid in immune evasion by mycobacteria. Interacts with human Toll-like receptor 2 (TLR2) and triggers functional maturation of human dendritic cells (DCs), leading to secretion of IL- 4, IL-5 and IL-10 from CD4(+) T cells and induction of Th2 immune response. Maturation of DCs involves PI3K, ERK1/2, p38 MAPK and NF- kappa-B signaling pathways. (1459 aa)
mpt63Immunogenic protein Mpt63 (antigen Mpt63/MPB63) (16 kDa immunoprotective extracellular protein); Rv1926c, (MT1977, MTCY09F9.38), len: 159 aa. Mpt63 (alternate gene name: mpb63), immunogenic protein (see citations below), identical to MPT63|MPB63 from Mycobacterium bovis (159 aa). Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al., 2008). (159 aa)
Rv1930cRv1930c, MTCY09F9.34, len: 174 aa. Conserved hypothetical protein, similar to SC5F2A.30|AL049587 hypothetical protein from Streptomyces coelicolor (211 aa),FASTA scores: opt: 307, E(): 2.8e-13, (54.8% identity in 84 aa overlap). Some similarity to M. tuber culosis hypothetical protein Rv0052|MTCY21D4.15 (43% identity in 93 aa overlap). (174 aa)
Rv1931cProbable transcriptional regulatory protein; Controls the expression of genes important for virulence. (259 aa)
Rv1949cRv1949c, (MTCY09F9.15), len: 319 aa. Conserved hypothetical protein, partial ORF. Rv1949c and Rv1950c|MTCY09F9.14 are similar but frameshifted with respect to Rv2077c|MTCY49.16C|Q10685 hypothetical 33.3 kd protein (323 aa), FASTA scores: opt: 459, E(): 2.8e-16,(54.8% identity in 157 aa overlap). Cosmid sequence appears to be correct, genomic sequence is also frameshifted in Mycobacterium bovis strain AF2122/97. Similar to Mycobacterium tuberculosis hypothetical proteins: Rv2542,Rv2077c, Rv2797c, Rv0963c, etc. (319 aa)
vapC14Possible toxin VapC14; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB14 (By similarity); Belongs to the PINc/VapC protein family. (103 aa)
vapC35Possible toxin VapC35. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB35.; Belongs to the PINc/VapC protein family. (135 aa)
Rv1975Rv1975, (MTV051.13), len: 221 aa. Conserved hypothetical protein, showing some similarity to AJ251435 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (193 aa). Predicted to be an outer membrane protein (See Song et al., 2008). (221 aa)
mpt64Immunogenic protein Mpt64 (antigen Mpt64/MPB64); Rv1980c, (MT2032, MTCY39.39), len: 228 aa. Mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below),identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al.,2008). (228 aa)
Rv1987Possible chitinase; Rv1987, (MTCY39.32c), len: 142 aa. Possible chitinase, similar to several e.g. P36909|CHIT_STRLI chitinase c precursor (619 aa) FASTA scores, opt: 324, E(): 1.2e-14, (39.5% identity in 129 aa overlap). (142 aa)
mazF6Toxin MazF6; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis partially inhibits cell growth and colony formation; its toxic effect is neutralized by coexpression with cognate antitoxin MazE6. Acts as an mRNA interferase on ssRNA, cleaving between the second and third bases in the sequences CUCCU and UUCCU. Further experiments demonstrate that it digests between the first and second bases of UCCUU, yielding a 5'- hydroxyl end; digests M.tuberculosis mRNA (in coding as well as the 5'- and 3'-UTR regions) and 23S rRNA, digests E.coli [...] (114 aa)
vapC15Toxin VapC15; Toxic component of a type II toxin-antitoxin (TA) system. Degrades total E.coli RNA, which is partially inhibited by cognate antitoxin VapB15. Upon expression in M.smegmatis inhibits colony formation, which is neutralized by coexpression with VapB15. (132 aa)
Rv2013Transposase; Rv2013, (MTCY39.04c), len: 159 aa. Transposase,shows similarity to N-terminal part of transposase and insertion element hypothetical proteins. Length changed since first submission (no clear start apparent). (159 aa)
Rv2014Transposase; Rv2014, (MTCY39.03c), len: 196 aa. Transposase,similar to insertion elements; possibly made by frameshifting with respect to Rv2013. Length changed since first submission. (196 aa)
Rv2033cRv2033c, (MTV018.20), len: 280 aa. Conserved hypothetical protein, similar to many. (280 aa)
lipTRv2045c, (MTV018.32c), len: 511 aa. LipT,carboxylesterase, similar to many. Contains PS00941 Carboxylesterases type-B signature 2. Contains PS00122 Carboxylesterases type-B serine active site; Belongs to the type-B carboxylesterase/lipase family. (511 aa)
Rv2054Conserved protein; Rv2054, (MTCY63A.06c), len: 237 aa. Conserved protein, similar to many. Contains IPR002925 Dienelactone hydrolase domain. (237 aa)
mazF7Possible toxin MazF7; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE7. Probably an endoribonuclease (By similarity); Belongs to the PemK/MazF family. (136 aa)
blaCClass a beta-lactamase BlaC; Extended spectrum beta-lactamase (ESBL) that inactivates beta-lactam antibiotics by hydrolyzing the amide group of the beta- lactam ring. Displays high levels of penicillinase and cephalosporinase activity as well as measurable activity with carbapenems, including imipenem and meropenem. Plays a primary role in the intrinsic resistance of M.tuberculosis to beta-lactam antibiotics. (307 aa)
Rv2077cRv2077c, (MTCY49.16c), len: 323 aa. Possible conserved transmembrane protein. Part of Mycobacterium tuberculosis protein family with Rv2542, Rv2079, Rv2797c,Rv0963c, Rv1949c. Hydrophobic stretches at C-terminus. (323 aa)
Rv2082Rv2082, (MTCY49.21), len: 721 aa. Conserved hypothetical protein. Similar to Mycobacterium tuberculosis Rv0029, and to Rv3899c and Rv3900c which may be frameshifted. (721 aa)
Rv2083Rv2083, (MTCY49.22), len: 314 aa. Conserved hypothetical protein. Similar to many e.g. Mycobacterium tuberculosis Rv3898c (110 aa) and Rv3897c (210 aa). (314 aa)
vapC37Possible toxin VapC37. Contains PIN domain; Probable toxic component of a type II toxin-antitoxin (TA) system. An RNase. Upon expression in M.smegmatis inhibits colony formation. The putative cognate antitoxin is VapB37. (144 aa)
prcBProteasome beta subunit PrcB; Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.tuberculosis proteasome is able to cleave oligopeptides not only after hydrophobic but also after basic, acidic and small neutral residues. In complex with the ATPase Mpa, degrades protein targets conjugated to a prokaryotic ubiquitin-like protein (Pup). Among the identified substrates of the M.tuberculosis proteasome are the pupylated FabD, PanB and Mpa proteins. One function of the proteasome is to contribute to M.tuberculosis ability [...] (291 aa)
Rv2141cConserved protein; Rv2141c, (MTCY270.27), len: 448 aa. Conserved protein. Shows some similarity to conserved hypothetical proteins and to acetylornithine deacetylase and succinyl-diaminopimelate desuccinylase and contains ArgE/dapE/ACY1/CPG2/yscS family signature 1 (PS00758). FASTA best: CBPS_YEAST P27614 carboxypeptidases precursor (576 aa) opt: 234, E(): 4.3e-08; (24.3% identity in 412 aa overlap). Previously named dapE2. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guima [...] (448 aa)
murCProbable UDP-N-acetylmuramate-alanine ligase MurC; Cell wall formation; Belongs to the MurCDEF family. (494 aa)
PE_PGRS38PE-PGRS family protein PE_PGRS38; Rv2162c, (MTCY270.06), len: 532 aa. PE_PGRS38,Member of M. tuberculosis PE_PGRS family (see citations below). FASTA score: Y03A_MYCTU Q 10637 hypothetical glycine-rich 49.6 kDa protein (603 aa) op t: 1798 z-score: 1220.0 E(): 0; (55.4% identity in 590 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (532 aa)
Rv2190cConserved hypothetical protein; Rv2190c, (MTV021.23c, MTCY190.01c), len: 385 aa. Conserved hypothetical protein; similar to other hypothetical mycobacterial proteins, including Rv1477,Rv1478, Rv1566c, Rv0024, that are similar to protein p60 precursors from Listeria e.g. Q018 38|P60_LISSE protein p60 precursor (invasion-associated protein) (524 aa). FASTA scores: gp|Z80233|MTCY10H4_25 (281 aa) opt: 290, E(): 6.9e-05; 37.0% identity in 127 aa overlap and sp|Q01838|P60_LISSE protein P60 precursor (523 aa) opt: 268, E(): 0.00071; 38.5% identity in 104 aa overlap; Belongs to the peptidase C [...] (385 aa)
glnA1Glutamine synthetase GlnA1 (glutamine synthase) (GS-I); Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Also able to use GTP. D-glutamate is a poor substrate, and DL-glutamate shows about 50% of the standard specific activity. Also plays a key role in controlling the ammonia levels within infected host cells and so contributes to the pathogens capacity to inhibit phagosome acidification and phagosome-lysosome fusion. Involved in cell wall biosynthesis via the production of the major component p [...] (478 aa)
vapC16Possible toxin VapC16; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB16 (By similarity). Belongs to the PINc/VapC protein family. (141 aa)
ptpAPhosphotyrosine protein phosphatase PtpA (protein-tyrosine-phosphatase) (PTPase) (LMW phosphatase); Mediates host-pathogen interaction and interferes with vesicular trafficking in the infected macrophage. Inhibits host phagolysosomal fusion in M.tuberculosis-infected macrophages to promote bacteria survival. Dephosphorylates host VPS33B protein, which induces a block of the host phagosome maturation within macrophage cells. Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates; Belongs to the low molecular weight phosphotyrosine prot [...] (163 aa)
Rv2264cRv2264c, (MTV022.14c), len: 592 aa. Conserved hypothetical Pro-rich protein, similar to hypothetical proteins Rv0312 (MTCY63.17, 620 aa and Rv0350) that has highly Pro-, Thr-rich C-terminus. Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. FASTA scores: Z96800|MTCY63_17 Mycobacterium tuberculosis cosmid (620 aa) opt: 1075, E(): 8.8e-24; (38.9% identity in 627 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). (592 aa)
cut2Probable cutinase Cut2; Rv2301, (MTCY339.08c), len: 230 aa. Probable cut2 (alternate gene name: cfp25), cutinase, highly similar to others from Mycobacteria tuberculosis e.g. MTCY13E12.04|Rv3451|O06318|CUT3_MYCTU (247 aa), FASTA scores: opt: 569, E(): 2.3e-27, (45.3% identity in 223 aa overlap); MT2037|MTCY39.35|RV1984C|Q10837|CUT1_MYCTU (217 aa), FASTA scores: opt: 383, E(): 3.4e-16 (42.9% identity in 217 aa overlap); O69691|Rv3724|MTV025.072 putative cutinase precursor (187 aa), FASTA scores: opt: 248, E(): 4.3e-08, (41.85% identity in 172 aa overlap); etc. Also similar to few others [...] (230 aa)
Rv2306ARv2306A, len: 197 aa. Possible conserved membrane protein, similar to several hypothetical membrane proteins from Mycobacterium tuberculosis and Streptomyces coelicolor, e.g. Rv0625c|P96915|Y625_MYCTU hypothetical 25.2 KDA protein from Mycobacterium tuberculosis (246 aa),FASTA scores: opt: 410, E(): 2.7e-17, (53.25% identity in 139 aa overlap). First 140 aa show high similarity, this then decreases but continues in next ORF Rv2306B,suggesting a frameshift near nt 2577473. However the sequence has been checked and no error found. The sequence is identical in CDC1551 and Mycobacterium bo [...] (197 aa)
Rv2306BRv2306B, len: 144 aa. Possible conserved membrane protein, similar to C-terminal part of several hypothetical membrane proteins from Mycobacterium tuberculosis and Streptomyces coelicolor e.g. P96915|Y625_MYCTU|RV0625c hypothetical 25.2 KDA protein from Mycobacterium tuberculosis (246 aa), FASTA scores: opt: 480, E(): 5e-24,(77.15% identity in 92 aa overlap). Could be a continuation of Rv2306A suggesting there may be a frameshift near nt 2577473. The C-terminal part is longer than Rv0625c and the 3'-end of gene overlaps Rv2307c, so maybe a further framehift. However, sequence has been [...] (144 aa)
plcCRv2349c, (MT2414, MTCY98.18c), len: 508 aa. Probable plcC, phospolipase C 3 (see citations below), similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 1013, E(): 9.3e-54, (38.85% identity in 525 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 630, E(): 1.5e-30, (35.15% identity in 535 aa overlap); Q9S816|T12J13.18|T21P5.4 putative phospholipase from Arabidopsis thaliana (Mouse-ear cr [...] (508 aa)
plcBMembrane-associated phospholipase C 2 PlcB; Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity. (512 aa)
plcAMembrane-associated phospholipase C 1 PlcA (MTP40 antigen); Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity; Belongs to the bacterial phospholipase C family. (512 aa)
glySProbable glycyl-tRNA synthetase GlyS (glycine--tRNA ligase) (GLYRS); Catalyzes the attachment of glycine to tRNA(Gly). Belongs to the class-II aminoacyl-tRNA synthetase family. (463 aa)
cfp2Low molecular weight antigen CFP2 (low molecular weight protein antigen 2) (CFP-2); May play a role in the development of protective immune responses; Belongs to the MTB12 family. (168 aa)
rpfDProbable resuscitation-promoting factor RpfD; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Belongs to the transglycosylase family. Rpf subfamily. (154 aa)
PE_PGRS41PE-PGRS family protein PE_PGRS41; Rv2396, (MTCY253.25c), len: 361 aa. PE_PGRS41,member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Also known as aprC, acid and phagosome regulated protein C,restricted to M. tuberculosis complex (See Abramovitch et al., 2011). Contains PS00583 pfkB family of carbohydrate kinases signature 1. Predicted to be an outer membrane protein (See Song et al., 2008). (361 aa)
eisEnhanced intracellular survival protein Eis,GCN5-related N-acetyltransferase; Effector that is released into the host cell and affects host immune responses; it negatively modulates inflammation, macrophage autophagy, and cell death through redox-dependent signaling. Acts as an acetyltransferase. Acetylates 'Lys-55' of dual-specificity protein phosphatase 16 (DUSP16)/mitogen-activated protein kinase phosphatase-7 (MKP-7), a JNK- specific phosphatase; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced [...] (402 aa)
Rv2422Hypothetical protein; Rv2422, (MTCY428.25c), len: 90 aa. Hypothetical unknown protein. (90 aa)
Rv2424cProbable transposase; Rv2424c, (MTCY428.23), len: 333 aa. Probable transposase for IS1558, similar to is element proteins e.g. AL021957|Rv2177c|MTV021_10 from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 1491, E(): 6.2e-87, (98.6% identity in 221 aa overlap); P19780|YIS1_STRCO hypothetical insertion element IS110 from Streptomyces coelicolor (45 aa), FASTA scores: opt: 203, E(): 1.7e-05; (27.3% identity in 238 aa overlap); etc. Contains PS01159 WW/rsp5/WWP domain signature. (333 aa)
rpfEProbable resuscitation-promoting factor RpfE; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Belongs to the transglycosylase family. Rpf subfamily. (172 aa)
echA14Probable enoyl-CoA hydratase EchA14 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Could possibly oxidize fatty acids using specific components. (256 aa)
vapC38Possible toxin VapC38. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB38 (By similarity). (141 aa)
Rv2514cRv2514c, (MTCY07A7.20c), len: 153 aa. Conserved hypothetical protein, showing some similarity to Q9PG05|XF0497 hypothetical protein from Xylella fastidiosa (155 aa), FASTA scores: opt: 215, E(): 1.4e-07, (30.6% identity in 160 aa overlap). (153 aa)
ldtBProbable L,D-transpeptidase LdtB; Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. (408 aa)
PE26Rv2519, (MTV009.04), len: 492 aa. PE26, Member of the M. tuberculosis PE family (see citation below), highly similar to many e.g. Q50630|YP91_MYCTU|Rv2591|MT2668.1|MTCY227.10c (543 aa),FASTA scores: opt: 848, E(): 3e-30, (39.55% identity in 445 aa overlap). (492 aa)
vapC17Possible toxin VapC17; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB17 (By similarity). Belongs to the PINc/VapC protein family. (133 aa)
vapC39Possible toxin VapC39. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB39. (139 aa)
vapC18Possible toxin VapC18; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB18 (By similarity). Belongs to the PINc/VapC protein family. (137 aa)
vapC19Possible toxin VapC19; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB19; Belongs to the PINc/VapC protein family. (125 aa)
vapC40Possible toxin VapC40. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Its cognate antitoxin is VapB40. (134 aa)
vapC41Possible toxin VapC41. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB41. (146 aa)
chiZPossible conserved membrane protein; Cell wall hydrolase that modulates cell division process. Probably acts by modulating FtsZ ring assembly. Murein hydrolase activity is targeted to sites of nascent peptidoglycan (PG) synthesis. Overproduction compromises midcell localization of FtsZ rings, but has no effect on the intracellular levels of FtsZ. (165 aa)
Rv2721cRv2721c, (MTCY05A6.42c, MTCY154.01c), len: 699 aa. Possible conserved transmembrane ala-, gly-rich protein,equivalent to Q49837|ML1002|U2235I possible conserved membrane protein from Mycobacterium leprae (687 aa), FASTA scores: opt: 2703, E(): 6.6e-135, (60.3% identity in 713 aa overlap). Shows some similaity to Q01377|CSP1 PS1 protein precursor (secreted protein) from Corynebacterium glutamicum (Brevibacterium flavum) (657 aa), FASTA scores: opt: 276, E(): 3.8e-07, (29.4% identity in 272 aa overlap); and Q9KIJ0 Rv2721c-like protein from Mycobacterium paratuberculosis (246 aa), FASTA s [...] (699 aa)
vapC21Possible toxin VapC21; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB21; Belongs to the PINc/VapC protein family. (138 aa)
Rv2765Rv2765, (MTV002.30), len: 245 aa. Probable ala-rich hydrolase, similar to various hydrolases or hypothetical proteins e.g. Q9KYM6|SC9H11.13c putative hydrolase from Streptomyces coelicolor (251 aa), FASTA scores: opt: 630,E(): 1.4e-33, (43.1% identity in 246 aa overlap); Q9A5T9|CC2358 dienelactone hydrolase family protein from Caulobacter crescentus (286 aa), FASTA scores: opt: 592,E(): 4.5e-31, (38.45% identity in 242 aa overlap); Q9FCF1|2SCD46.33 putative hydrolase (dienelactone hydrolase family) from Streptomyces coelicolor (254 aa), FASTA scores: opt: 500, E(): 3.9e-25, (37.7% iden [...] (245 aa)
aldSecreted L-alanine dehydrogenase Ald (40 kDa antigen) (TB43); Catalyzes the reversible reductive amination of pyruvate to L-alanine. However, since the physiological environment of M.tuberculosis has a neutral pH, it can be assumed that the enzyme catalyzes exclusively the formation of L-alanine. May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer; Belongs to the AlaDH/PNT family. (371 aa)
mazF9Toxin MazF9; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE9. Acts as an mRNA interferase, specifically cleaving between U and C in UAC sequences. May cleave its cognate antitoxin's gene. In E.coli expression with non- cognate antitoxins VapB27 and VapB40 partially neutralizes the toxin. Belongs to the PemK/MazF family. (118 aa)
vapC22Possible toxin VapC22; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits translation and colony formation. Its toxic effect on colony formation is neutralized by coexpression with cognate antitoxin VapB22; the effect on translation has not been tested but is probably neutralized also. (130 aa)
echA16Probable enoyl-CoA hydratase EchA16 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv2831, (MTCY16B7.11c), len: 249 aa. Probable echA16, enoyl-CoA hydratase, similar to others e.g. O23468|AT4G16210 from Arabidopsis thaliana (Mouse-ear cress) (244 aa), FASTA scores: opt: 491, E(): 7.3e-25,(42.1% identity in 190 aa overlap); Q98LI4|MLL1009 from Rhizobium loti (Mesorhizobium loti) (258 aa), FASTA scores: opt: 491, E(): 7.6e-25, (40.75% identity in 248 aa overlap); O07137|ECH8_MYCLE|ML2402|MLCB1306.05c from Mycobacterium leprae (257 aa), FASTA scores: opt: 478, E(): 5.3e-24, [...] (249 aa)
infBProbable translation initiation factor if-2 InfB; One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity); Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. (900 aa)
cysGRv2847c, (MTCY24A1.10), len: 405 aa. Possible cysG,multifunctional enzyme, siroheme synthase containing uroporphyrin-III c-methyltransferase, precorrin-2 oxidase and ferrochelatase. C-terminus highly similar to many uroporphyrin-III c-methyltransferases e.g. Q51720|COBA uroporphyrinogen III methyltransferase from Propionibacterium freudenreichii (257 aa), FASTA scores: opt: 776, E(): 1.5e-39, (48.95% identity in 243 aa overlap); Q9HMY4|UROM|VNG2331G S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Halobacterium sp. strain NRC-1 (246 aa), FASTA scores: opt: 704, E(): [...] (405 aa)
Rv2850cRv2850c, (MTCY24A1.07), len: 629 aa. Possible magnesium-chelatase, highly similar (but with gaps) to magnesium-chelatases from notably photosynthetic organisms involved in chlorophyll biosynthesis e.g. Q9RJ18|SCI8.35c putative chelatase from Streptomyces coelicolor (672 aa),FASTA scores: opt: 1941, E(): 2.1e-85, (54.65% identity in 675 aa overlap); Q9HZQ5|PA2942 probable magnesium chelatase from Pseudomonas aeruginosa (338 aa), FASTA scores: opt: 991, E(): 2.7e-40, (49.45% identity in 368 aa overlap); O33549|BCHI mg protoporphyrin IX chelatase subunit from Rhodobacter sphaeroides (Rhod [...] (629 aa)
vapC23Possible toxin VapC23; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB23 (By similarity). Belongs to the PINc/VapC protein family. (126 aa)
vapC43Possible toxin VapC43. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB43.; Belongs to the PINc/VapC protein family. (147 aa)
mpt83Cell surface lipoprotein Mpt83 (lipoprotein P23); Recombinant, non-modified protein stimulates secretion of cytokines (TNF-alpha, IL-6 and IL-12p40) by mouse macrophage cell lines in a TLR2-dependent fashion, which leads to increased host innate immunity responses against the bacterium. Serves as a strong human and mouse antigen T cell antigen during M.tuberculosis infection, inducing strong IFN-gamma expression. (220 aa)
mpt70Major secreted immunogenic protein Mpt70; Rv2875, (MTCY274.06), len: 193 aa. Mpt70 (alternate gene name: mpb70), major secreted immunogenic protein MPT70 precursor (see citations below). Also similar to downstream ORF Q10790|MP83_MYCTU|MPT83|MPB83|Rv2873|MT2940|MTCY274.04 cell surface lipoprotein MPT83 precursor (lipoprotein P23) (220 aa), FASTA scores: opt: 806, E(): 1.2e-40, (70.25% identity in 185 aa overlap). Belongs to the MPT70 / MPT83 family. Generally found as a monomer; homodimer in culture fluids. (193 aa)
mpt53Soluble secreted antigen Mpt53 precursor; Disulfide oxidoreductase that catalyzes the oxidation of reduced, unfolded secreted proteins to form disulfide bonds. Despite a weak homology to thioredoxin this cannot serve as a substrate for thioredoxin reductase. (173 aa)
lppWRv2905, (MTCY274.36), len: 314 aa. Probable lppW,conserved ala-rich lipoprotein, with slight similarity to beta-lactamases and hypothetical proteins e.g. Q9S1P7|SCJ9A.23 hypothetical 36.3 KDA protein from Streptomyces coelicolor (336 aa), FASTA scores: opt: 222,E(): 2.8e-06, (25.5% identity in 298 aa overlap); O69914|SC3C8.01 putative secreted protein from Streptomyces coelicolor (302 aa), FASTA scores: opt: 201, E(): 5.1e-05,(24.9% identity in 257 aa overlap); P14559|BLAC_STRAL beta-lactamase precursor from Streptomyces albus G (314 aa), FASTA scores: opt: 113, E(): 3.3, (25.2% identi [...] (314 aa)
Rv2972cRv2972c, (MTCY349.15), len: 237 aa. Possible conserved membrane or exported protein, equivalent (but longer 52 aa) to O69461|MLCB1243.02 hypothetical 20.5 KDA protein from Mycobacterium leprae (180 aa), FASTA scores: opt: 581, E(): 8.2e-32, (55.75% identity in 174 aa overlap). Also similar to membrane or exported proteins e.g. Q9F2P3|SCE41.16C putative lipoprotein from Streptomyces coelicolor (258 aa), FASTA scores: opt: 498,E(): 4.1e-26, (44.08% identity in 186 aa overlap); Q99QB5|SCP1.323C putative secreted protein from Streptomyces coelicolor (219 aa), FASTA scores: opt: 329,E(): 8. [...] (237 aa)
cfp6Rv3004, (MT3084.1, MTV012.18), len: 112 aa. Cfp6,low molecular weight protein antigen 6 (CFP-6) (See Bhaskar et al., 2000). Weak homology with Q9RKZ5|SC6D7.02 putative membrane protein from Streptomyces coelicolor (156 aa),FASTA scores: opt: 109, E(): 0.78, (39.4% identity in 122 aa overlap). Caution: the initiator methionine may be further upstream making the sequence a precursor. Predicted to be an outer membrane protein (See Song et al., 2008). (112 aa)
Rv3096Rv3096, (MTCY164.07), len: 379 aa. Hypothetical protein, with slight similarity to several proteins e.g. Q09671|OYEB_SCHPO|SPAC5H10.10 putative NADPH dehydrogenase C5H10.10 (old yellow enzyme homolog) from Schizosaccharomyces pombe (Fission yeast) (392 aa), FASTA scores: opt: 125, E(): 1.1, (25.45% identity in 165 aa overlap); and Q12603|XYNA_DICTH beta-1,4-xylanase (endo-1,4-beta-xylanase) from Dictyoglomus thermophilum (352 aa), FASTA scores: opt: 124, E(): 1.2, (25.65% identity in 195 aa overlap); etc. Contains glycosyl hydrolases family 5 signature (PS00659). Predicted to be an out [...] (379 aa)
Rv3103cHypothetical proline-rich protein; Rv3103c, (MTCY164.13c), len: 145 aa. Hypothetical unknown pro-rich protein, with some similarity to Proline-rich proteins e.g. Q39789 proline-rich cell wall protein from Gossypium hirsutum (Upland cotton) (214 aa),FASTA scores: opt: 267, E(): 0.00014, (40% identity in 110 aa overlap). Equivalent to AAK47525 from M. tuberculosis strain CDC1551 (158 aa) but shorter 13 aa. (145 aa)
devRTwo component transcriptional regulatory protein DevR (probably LuxR/UhpA-family); Member of the two-component regulatory system DevR/DevS (also called DosR/DosS) involved in onset of the dormancy response. Regulates an approximately 48-member regulon. When phosphorylated binds and activates the promoter of DevR regulon genes in response to hypoxia. The presence of target DNA increases stability of phospho-DevR in vitro. Activates its own transcription under hypoxic but not aerobic conditions, probably binds as a dimer to tandem binding sites within the devR and hspX promoters. Accepts [...] (217 aa)
PPE50Rv3135, (MTCY03A2.23c), len: 132 aa. PPE50, Member of the Mycobacterium tuberculosis Ala-, Gly-rich PPE family, similar to P95190|Rv3136|MTCY03A2.22c (380 aa),FASTA scores: opt: 494, E(): 6.7e-25, (57.25% identity in 131 aa overlap) (next ORF downstream), MTY21C12_9,MTCY3C7_24, MTCI125_27, MTV049_12, MTV049_9, MTV049_11,MTCY274_24 etc. (132 aa)
PPE51Rv3136, (MTCY03A2.22c), len: 380 aa. PPE51, Member of the Mycobacterium tuberculosis Ala-, Gly-rich PPE family, similar to Q9AGF0|Ov2770c Rv2770c-like protein from M. microti (397 aa), FASTA scores: opt: 917, E(): 9e-41,(46.15% identity in 388 aa overlap); O33312|Rv2770c|MTV002.35c, MTV002_36, MTCI125_26,MTCY10G2_10, MTCI364_8, MTV049_28, MTV049_29, etc. (380 aa)
vapC49Hypothetical alanine rich protein; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB49 (By similarity). (144 aa)
sahHProbable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase); May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine. (495 aa)
lpqCRv3298c, (MTCY71.38c), len: 304 aa. Possible lpqC,esterase lipoprotein, equivalent to Q9CCL5|LPQC|ML0715 putative secreted hydrolase from Mycobacterium leprae (304 aa), FASTA scores: opt: 1543, E(): 1.3e-87, (71.6% identity in 303 aa overlap); and Q49658|B1308_F2_43 tubulin family protein from Mycobacterium leprae (302 aa), FASTA scores: opt: 1541, E(): 1.7e-87, (72.0% identity in 300 aa overlap). Also similar to Q9I5Z3|PA0543 hypothetical protein from Pseudomonas aeruginosa (322 aa), FASTA scores: opt: 439, E(): 8.9e-20, (32.3% identity in 319 aa overlap); Q9F2K9|SCH63.19c putative se [...] (304 aa)
sapMAcid phosphatase (acid phosphomonoesterase) (phosphomonoesterase) (glycerophosphatase); Virulence factor that plays an important role in blocking phagosome-lysosome fusion and thus participates in the intracellular survival of the pathogen. Acts as a phosphatase that dephosphorylates phosphatidylinositol 3-phosphate (PI3P), a membrane trafficking regulatory lipid essential for phagosomal acquisition of lysosomal constituents. Therefore, SapM eliminates PI3P from the phagosomal membrane by catalyzing its hydrolysis, and thus contributes to inhibition of phagosome maturation. Also interf [...] (299 aa)
vapC44Possible toxin VapC44. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB44 (By similarity). (142 aa)
Rv3327Rv3327, (MTV016.27), len: 570 aa. Probable fusion protein. Indeed, N-terminal part corresponds to entire O07269 transposase of IS1547 (383 aa), and C-terminal part identical to MTCI249B.03c (210 aa). N-terminal part is identical to MTV042_7 (188 aa); C-terminal part (aa 378-570) is similar to hypothetical 20.5 kDa protein from Escherichia coli P76222|YNJA_ECOLI (182 aa), FASTA scores: opt: 292, E(): 5.3e-11, (32.6% identity in 181 aa overlap). (570 aa)
PPE54PPE family protein PPE54; Probably plays a role in host phagosome maturation arrest. (2523 aa)
PPE55Rv3347c, (MTV004.03c), len: 3157 aa. PPE55, Member of the Mycobacterium tuberculosis PPE family, Gly-, Ala-,Asn-rich protein. Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551, e.g. O50379|Rv3350c|MTV004.07c (3716 aa), FASTA scores: opt: 6497, E(): 0, (61.65% identity in 3756 aa overlap); and other upstream ORFs MTV004_5, MTY13E10_15, MTCY28_16,MTCY63_9, MTY13E10_17, MTCY180_1; etc. Predicted possible vaccine candidate (See Zvi et al., 2008). (3157 aa)
Rv3348Probable transposase; Rv3348, (MTV004.04), len: 163 aa. Probable transposase, partially similar to several insertion elements e.g. P19834|YI11_STRCL insertion element IS116 hypothetical 44.8 KDA protein (similar to IS900 of Mycobacterium paratuberculosis) from Streptomyces clavuligerus (399 aa), FASTA scores: opt: 146, E(): 0.016,(29.1% identity in 158 aa overlap). (163 aa)
PPE56Rv3350c, (MTV004.07c), len: 3716 aa. PPE56, Member of the Mycobacterium tuberculosis PPE family of Gly-, Ala-,Asn-rich proteins, similar to many Mycobacterium tuberculosis proteins from strains H37Rv and CDC1551, e.g. O50378|Rv3347c|MTV004.03c (3157 aa), FASTA scores: opt: 6497, E(): 0, (61.65% identity in 3756 aa overlap); MTCY28_16, MTV050_2, MTY13E10_17, MTCY63_10, MTCY180_1,MTCY63_9, MTV050_1, MTV014_3, MTY13E10_15; etc. (3716 aa)
echA18Probable enoyl-CoA hydratase EchA18 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv3373, (MTV004.31), len: 213 aa. Probable echA18,enoyl-CoA hydratase, similar to others e.g. P97087|CRT from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (259 aa), FASTA scores: opt: 423,E(): 3.4e-20, (37.95% identity in 174 aa overlap); Q9X7Q4|SC5F2A.31c from Streptomyces coelicolor (257 aa),FASTA scores: opt: 399, E(): 1.2e-18, (45.05% identity in 171 aa overlap); BAB52005|MLL5584 from Rhizobium loti (Mesorhizobium loti) (257 aa), FASTA scores: opt: 38 [...] (213 aa)
echA18.1Probable enoyl-CoA hydratase EchA18.1 (Enoyl hydrase) (Unsaturated acyl-CoA hydratase) (Crotonase); Rv3374, (MTV004.32), len: 82 aa. Probable echA18.1,enoyl-CoA hydratase C-terminus, similar to the C-terminus of several enoyl-CoA hydratases e.g. Q9I5I4|PA0745 from Pseudomonas aeruginosa (272 aa), FASTA scores: opt: 123,E(): 0.13, (34.55% identity in 81 aa overlap); P97087|CRT from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (259 aa),FASTA scores: opt: 115, E(): 0.45, (32.95% identity in 82 aa overlap); Q9I002|PA2841 from Pseudomonas aeruginosa (263 a [...] (82 aa)
vapC47Possible toxin VapC47. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits translation and colony formation. Its toxic effect on colony formation is neutralized by coexpression with cognate antitoxin VapB47; the effect on translation has not been tested but is probably neutralized also.; Belongs to the PINc/VapC protein family. (136 aa)
Rv3438Conserved protein; Rv3438, (MTCY77.10), len: 280 aa. Conserved protein,equivalent to Q9CCV6|ML0370 hypothetical protein from Mycobacterium leprae (289 aa), FASTA scores: opt: 1491,E(): 9.2e-81, (79.85% identity in 283 aa overlap); and highly similar (but shorter 41 aa) to Q49872|B229_F1_20 hypothetical 34.0 KDA protein from Mycobacterium leprae (324 aa), FASTA scores: opt: 1491, E(): 1e-80, (79.85% identity in 283 aa overlap). Shows some similarity to Q9KIU3|LIPA lipase from plasmid pAH114 uncultured bacterium (281 aa), FASTA scores: opt: 168, E(): 0.0081, (29.3% identity in 140 aa ove [...] (280 aa)
esxURv3445c, (MTCY77.17c), len: 105 aa. EsxU, ESAT-6 like protein (see citations below), showing weak similarity to O30373|VCD|PA2257 pyoverdine biosynthesis protein from Pseudomonas aeruginosa (215 aa), FASTA scores: opt: 103,E(): 5.6, (32.35% identity in 133 aa overlap). Seems to belong to the ESAT6 family. Start changed since first submission (-20 aa); Belongs to the WXG100 family. CFP-10 subfamily. (105 aa)
Rv3446cRv3446c, (MTCY77.18c), len: 404 aa. Hypothetical unknown ala-, val-rich protein. (404 aa)
cut3Rv3451, (MTCY13E12.04), len: 262 aa. Probable cut3,cutinase precursor, similar to others e.g. Q9KK87 from Mycobacterium avium (220 aa), FASTA scores: opt: 540, E(): 3.5e-24, (43.4% identity in 219 aa overlap); Q00298|CUTI_BOTCI|CUTA from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 214, E(): 2e-05,(31.45% identity in 210 aa overlap); Q9Y7G8 from Pyrenopeziza brassicae (203 aa), FASTA scores: opt: 203,E(): 8.5e-05, (31.05% identity in 190 aa overlap); P29292|CUTI_ASCRA from Ascochyta rabiei (223 aa), FASTA scores: opt: 155, E(): 0.054, (31.65% identity in 120 a [...] (262 aa)
cut4Rv3452, (MTCY13E12.05), len: 226 aa. Probable cut4,cutinase precursor, similar to other e.g. Q9KK87 from Mycobacterium avium (220 aa), FASTA scores: opt: 522, E(): 7.3e-24, (46.6% identity in 221 aa overlap); P30272|CUTI_MAGGR|CUT1 from Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) (228 aa), FASTA scores: opt: 205, E(): 3.8e-05, (29.25% identity in 164 aa overlap); Q00298|CUTI_BOTCI|CUTA from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 204, E(): 3.9e-05,(33.5% identity in 209 aa overlap); etc. Similar to other proteins from Mycobacterium tubercu [...] (226 aa)
Rv3483cPossible exported protein; Rv3483c, (MTCY13E12.36c), len: 220 aa. Possible exported protein, similar to Q9CC94|ML1099 putative lipoprotein from Mycobacterium leprae (202 aa), FASTA scores: opt: 276, E(): 1.4e-08, (33.1% identity in 148 aa overlap). Also showing similarity with Mycobacterium tuberculosis proteins Q11065|LPRE_MYCTU|LPRE|Rv1252c|MT1291|MTCY50.30. putative lipoprotein precursor (202 aa), FASTA scores: opt: 276,E(): 1.4e-08, (29.5% identity in 200 aa overlap); O53445|Rv1097c|MTV017.50c hypothetical 29.9 KDA protein (293 aa), FASTA scores: opt: 161, E(): 0.047, (25.4% identi [...] (220 aa)
PE_PGRS54PE-PGRS family protein PE_PGRS54; Rv3508, (MTV023.15), len: 1901 aa. PE_PGRS54, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. downstream O53559|Rv3514|MTV023.21 (1489 aa),FASTA scores: opt: 6598, E(): 0, (71.05% identity in 1533 aa overlap). Equivalent to AAK47971 from Mycobacterium tuberculosis strain CDC1551 (1384 aa) but shorter 13 aa and with some minor differences between the proteins. Contains five PS00583 pfkB family of carbohydr [...] (1901 aa)
PE_PGRS56PE-PGRS family protein PE_PGRS56; Rv3512, (MTV023.19), len: 1079 aa. PE_PGRS56, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK47974|MT3615.3 (1217 aa) FASTA scores: opt: 3688, E(): 4.5e-130, (53.95% identity in 1136 aa overlap); and downstream O53559|Rv3514|MTV023.21 (1489 aa), FASTA scores: opt: 3611, E(): 3.6e-127, (53.15% identity in 1195 aa overlap); etc. Frameshifted PGRS protein, could be continuation of upstream MTV023.18, but no err [...] (1079 aa)
lpqFRv3593, (MTCY6F7.01c), len: 452 aa. Probable lpqF,conserved lipoprotein, equivalent to Q9CBI7|MPQF|ML1923 probale secreted protein from Mycobacterium leprae (454 aa), FASTA scores: opt: 2465, E(): 5.7e-144, (79.15% identity in 451 aa overlap). Also similar to Q9KJ91 hypothetical 47.1 KDA protein from Streptomyces clavuligerus (430 aa), FASTA scores: opt: 609, E(): 5.2e-30, (30.3% identity in 350 aa overlap); and some similarity with putative beta-lactamases e.g. Q9RYR7|DRA0241 beta lactamase-related protein from Deinococcus radiodurans (499 aa), FASTA scores: opt: 322,E(): 2.5e-12, (28 [...] (452 aa)
Rv3604cRv3604c, (MTCY07H7B.18), len: 397 aa. Probable conserved ala-, arg-, pro-rich transmembrane protein,equivalent to O69526|MLCB2548.03c|ML0228 putative membrane protein from Mycobacterium leprae (432 aa), FASTA scores: opt: 869, E(): 2.9e-31, (59.7% identity in 432 aa overlap). Contains two possible membrane-spanning domains. N-terminus shortened since first submission (previously 462 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (397 aa)
espAESX-1 secretion-associated protein A, EspA; Required for secretion of EsxA (ESAT-6) and EsxB (CFP-10) and for virulence. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. (392 aa)
PPE65PPE family protein PPE65; Rv3621c, (MTCY15C10.31, MTCY07H7B.01), len: 413 aa. PPE65, Member of the Mycobacterium tuberculosis PPE family,ala-, gly-rich proteins, similar to many e.g. Q10813|YS92_MYCTU|Rv2892c|MT2959|MTCY274.23c (408 aa) FASTA scores: opt: 955, E(): 1.8e-42, (44.45% identity in 423 aa overlap). (413 aa)
Rv3630Rv3630, (MTCY15C10.22c), len: 431 aa. Probable conserved integral membrane, highly similar to P71789|YF10_MYCTU|Rv1510|MTCY277.32 hypothetical 44.3 KDA protein from Mycobacterium tuberculosis (432 aa) FASTA scores: opt: 1940, E(): 2.3e-103, (70.75% identity in 424 aa overlap). Note that N-terminal end is highly similar to AAK45825|MT1558 hypothetical 18.1 KDA protein from Mycobacterium tuberculosis strain CDC1551 (172 aa) FASTA scores: opt: 649, E(): 4.2e-30, (61.65% identity in 167 aa overlap); and C-terminal end is highly similar to AAK45826|MT1560 hypothetical 25.8 KDA protein from [...] (431 aa)
Rv3668cPossible protease; Rv3668c, (MTV025.016c), len: 232 aa. Possible protease (and more specifically a putative alkaline serine protease, equivalent to Q9CB98|ML2295 hypothetical protein from Mycobacterium leprae (234 aa), FASTA scores: opt: 1249, E(): 7.4e-66, (77.5% identity in 231 aa overlap). Also similar at C-terminal end with many proteases e.g. O86984 alkaline serine protease precursor from Thermomonospora fusca (368 aa), FASTA scores: opt: 190,E(): 0.00056, (28.9% identity in 173 aa overlap); Q55353|SAPII alkaline serine protease II from Streptomyces sp (382 aa), FASTA scores: opt: [...] (232 aa)
vapC48Possible toxin VapC48. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB48.; Belongs to the PINc/VapC protein family. (145 aa)
cut5aRv3724A, (MTV025.072), len: 80 aa. Probable cut5a,truncated cutinase precursor, similar to N-terminal end of others e.g. Q9KK87 serine esterase cutinase from Mycobacterium avium (220 aa), FASTA scores: opt: 202, E(): 1.5e-06, (56.45% identity in 62 aa overlap); Q9XB09|RVD2-RV1758 protein (fragment) from Mycobacterium bovis BCG (143 aa), FASTA scores: opt: 200, E(): 1.5e-06,(61.4% identity in 57 aa overlap); and Q00298|CUTI_BOTCI|CUTA cutinase precursor from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 108, E(): 2.2, (40.4% identity in 52 aa overlap). Also high [...] (80 aa)
cut5bRv3724B, (MTV025.072), len: 187 aa. Probable cut5b,truncated cutinase, similar to C-terminal end of others e.g. Q9XB09|RVD2-RV1758 protein (fragment) from Mycobacterium bovis BCG (143 aa) FASTA scores: opt: 335,E(): 3.4e-12, (53.25% identity in 92 aa overlap); Q9KK87 serine esterase cutinase from Mycobacterium avium (220 aa),FASTA scores: opt: 251, E(): 2.5e-07, (44.05% identity in 168 aa overlap). Also similar to proteins from Mycobacterium tuberculosis e.g. O06793|Rv1758|MTCY28.24 hypothetical 17.9 KDA protein (174 aa), FASTA scores: opt: 641, E(): 2.5e-29, (57.25% identity in 166 aa [...] (187 aa)
lpqH19 kDa lipoprotein antigen precursor LpqH; Based on its structure might be involved in ligand transport (By similarity); Belongs to the mycobacterial 19 kDa antigen family. (159 aa)
Rv3766Hypothetical protein; Rv3766, (MTV025.114), len: 229 aa. Hypothetical unknown protein. Segment 183 to 229 highly similar to C-terminal part of O06288|Rv3594|MTCY07H7B.28c conserved hypothetical protein from Mycobacterium tuberculosis (275 aa), FASTA scores: opt: 128, E(): 0.92, (46.8% identity in 47 aa overlap). (229 aa)
fbpDMPT51/MPB51 antigen; May have a role in host tissue attachment, whereby ligands may include the serum protein fibronectin and small sugars. (299 aa)
fbpADiacylglycerol acyltransferase/mycolyltransferase Ag85A; The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha- trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-treh [...] (338 aa)
pirGExported repetitive protein precursor PirG (cell surface protein) (EXP53); Surface-exposed protein required for multiplication and intracellular growth; To M.leprae 28 kDa antigen. (284 aa)
Rv3844Possible transposase; Rv3844, (MTCY01A6.25), len: 163 aa. Possible transposase, identical to P96234|Rv3348|MTV004.04 putative transposase from Mycobacterium tuberculosis. Also some similarity with others e.g. N-terminal part of P19834|YI11_STRCL insertion element IS116 hypothetical 44.8 KDA protein from Streptomyces clavuligerus (399 aa) FASTA scores: opt: 146, E(): 0.017, (29.1% identity in 158 aa overlap). (163 aa)
Rv3845Hypothetical protein; Rv3845, (MTCY01A6.24c), len: 119 aa. Hypothetical unknown protein. Contains PS01137 Hypothetical YBL055c/yjjV family signature 1. (119 aa)
sodASuperoxide dismutase [FE] SodA; Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. (207 aa)
espRESX-1 transcriptional regulatory protein EspR; Virulence regulator that has both architectural and regulatory roles. Impacts cell wall functions and pathogenesis through regulation of multiple genes, including the espACD operon, which is a key ESX-1 component. Influences target gene expression positively or negatively, depending on its binding position relative to the genes it controls. Acts by binding directly to the DNA. May play a central role in regulating virulence gene expression. (132 aa)
espERv3864, (MTCY01A6.04c), len: 402 aa. EspE, ESX-1 secretion-associated protein, similar to Q49722|ML0405|B1620_C2_213|MLCL383.01 hypothetical 40.8 KDA protein from Mycobacterium leprae (394 aa) FASTA scores: opt: 397, E(): 1.2e-12, (31.0% identity in 410 aa overlap). Also similar to various proteins from several organisms e.g. Q9VYF9|CG12723 hypothetical protein from Drosophila melanogaster (Fruit fly) (450 aa), FASTA scores: opt: 291,E(): 2.3e-07, (34.6% identity in 130 aa overlap); Q98UE3 procollagen ALPHA1(III) (fragment) from Xenopus laevis (African clawed frog) (117 aa) FASTA score [...] (402 aa)
esxB10 kDa culture filtrate antigen EsxB (LHP) (CFP10); A secreted protein. Acts as a strong host (human) T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA; native protein binds poorly to artificial liposomes in the absence or presence of EsxA. EsxA and EsxA-EsxB are cytotoxic to pneumocytes. EsxB (and EsxA-EsxB but not EsxA alone) activates human neutrophils; EsxB transiently induces host (human) intracellular Ca(2+) mobility in a dose-depend [...] (100 aa)
espJESX-1 secretion-associated protein EspJ. Conserved alanine rich protein; Could be involved in regulation of growth and intracellular survival. (280 aa)
espBSecreted ESX-1 substrate protein B, EspB. Conserved alanine and glycine rich protein; Required for host-cell death and may support an EsxA- independent virulence function. Secreted processed form of EspB binds to phosphatidic acid and phosphatidylserine. Inhibits IFN-gamma-induced autophagy in murine macrophages. (460 aa)
Rv3897cRv3897c, (MTCY15F10.15), len: 210 aa. Conserved hypothetical protein, highly similar in part to Q10691|YK83_MYCTU|Rv2083|MT2145|MTCY49.22 hypothetical 30.8 KDA protein from Mycobacterium tuberculosis (314 aa) FASTA scores: opt: 815, E(): 4.7e-26, (73.05% identity in 167 aa overlap). Similarity to MTCY49.22 suggests that this is a continuation of MTCY15F10.14. There is a frameshift mutation near 3'-end with respect to this sequence as well,similarity to MTCY49.22 continues in an overlapping ORF. Sequence appears to be correct. (210 aa)
Rv3898cRv3898c, (MTCY15F10.14), len: 110 aa. Conserved hypothetical protein. Highly similar, but in part, to Q10691|YK83_MYCTU|Rv2083|MT2145|MTCY49.22 hypothetical 30.8 KDA protein from Mycobacterium tuberculosis (314 aa) FASTA scores: opt: 204, E(): 0.00042, (50.6% identity in 81 aa overlap). Similarity suggests it should be in frame with next ORF and that the stop codon could be read through, the sequence appears to be correct. Homology lost upstream at 15138 gatc sequence may suggest discontinuity due to chimerism in cY15F10 or cY49. (110 aa)
Rv3899cRv3899c, (MTCY15F10.13), len: 410 aa. Conserved hypothetical protein, similar in part to proteins from Mycobacterium tuberculosis strains H37Rv and CDC1551. Region between aa 29-80 is strictly identical to P96909 hypothetical 15.1 KDA protein (fragment) (143 aa) FASTA scores: opt: 562, E(): 4e-16, (69.0% identity in 142 aa overlap); and the N-terminal end is highly similar, but longer 65 aa, to O07266 hypothetical 13.7 KDA protein (fragment) (143 aa), FASTA scores: opt: 562, E(): 4e-16,(69.0% identity in 142 aa overlap). Highly similar to C-terminal end of Q10690|YK82_MYCTU|Rv2082|MTCY [...] (410 aa)
Your Current Organism:
Mycobacterium tuberculosis H37Rv
NCBI taxonomy Id: 83332
Other names: M. tuberculosis H37Rv, Mycobacterium sp. H37Rv, Mycobacterium tuberculosis str. H37Rv, Mycobacterium tuberculosis strain H37Rv
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