Rv1683, (MTCI125.05), len: 999 aa. Possible bifunctional long-chain acyl-CoA synthase and lipase. Equivalent to Z95117|MLCB1351_21 possible long-chain acyl-CoA synthase from Mycobacterium leprae (1002 aa) (85.6% identity in 1002 aa overlap). Weakly similar to FATP_MOUSE|Q60714 long-chain fatty acid transport protein (646 aa), fasta scores: opt: 331, E(): 5e-08, (24.8% identity in 630 aa overlap). Also similar to O35488|AF033031 Mouse very-long-chain acyl-CoA synthetase (620 aa), fasta scores: opt: 435, E(): 2.2e-12, (24.8% identity in 545 aa overlap). Weakly similar to Mycobacterium tu [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Rv1683, (MTCI125.05), len: 999 aa. Possible bifunctional long-chain acyl-CoA synthase and lipase. Equivalent to Z95117|MLCB1351_21 possible long-chain acyl-CoA synthase from Mycobacterium leprae (1002 aa) (85.6% identity in 1002 aa overlap). Weakly similar to FATP_MOUSE|Q60714 long-chain fatty acid transport protein (646 aa), fasta scores: opt: 331, E(): 5e-08, (24.8% identity in 630 aa overlap). Also similar to O35488|AF033031 Mouse very-long-chain acyl-CoA synthetase (620 aa), fasta scores: opt: 435, E(): 2.2e-12, (24.8% identity in 545 aa overlap). Weakly similar to Mycobacterium tu [...]

Phenyloxazoline synthase MbtB (phenyloxazoline synthetase); Involved in the initial steps of the mycobactin biosynthetic pathway. Putatively couples activated salicylic acid with serine or threonine and cyclizes this precursor to the hydroxyphenyloxazoline ring system present in this class of siderophores. Essential for growth in macrophages; Belongs to the ATP-dependent AMP-binding enzyme family. MbtB subfamily.

Rv1683, (MTCI125.05), len: 999 aa. Possible bifunctional long-chain acyl-CoA synthase and lipase. Equivalent to Z95117|MLCB1351_21 possible long-chain acyl-CoA synthase from Mycobacterium leprae (1002 aa) (85.6% identity in 1002 aa overlap). Weakly similar to FATP_MOUSE|Q60714 long-chain fatty acid transport protein (646 aa), fasta scores: opt: 331, E(): 5e-08, (24.8% identity in 630 aa overlap). Also similar to O35488|AF033031 Mouse very-long-chain acyl-CoA synthetase (620 aa), fasta scores: opt: 435, E(): 2.2e-12, (24.8% identity in 545 aa overlap). Weakly similar to Mycobacterium tu [...]

Rv2380c, (MTCY22H8.05), len: 1682 aa. MbtE, peptide synthetase (see citations below), similar in part to several synthases e.g. O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 2635, E(): 1.9e-146, (36.8% identity in 1657 aa overlap); O05647|SNBDE virginiamycin S synthetase (fragment) from Streptomyces virginiae (1997 aa) FASTA scores: opt: 2580, E(): 1.6e-143, (40.65% identity in 1163 aa overlap); Q9R9I2|DHBF protein involved in siderophore production from Bacillus subtilis (2378 aa),FASTA scores: opt: 2388, E(): 3.6e-132, [...]

Rv1683, (MTCI125.05), len: 999 aa. Possible bifunctional long-chain acyl-CoA synthase and lipase. Equivalent to Z95117|MLCB1351_21 possible long-chain acyl-CoA synthase from Mycobacterium leprae (1002 aa) (85.6% identity in 1002 aa overlap). Weakly similar to FATP_MOUSE|Q60714 long-chain fatty acid transport protein (646 aa), fasta scores: opt: 331, E(): 5e-08, (24.8% identity in 630 aa overlap). Also similar to O35488|AF033031 Mouse very-long-chain acyl-CoA synthetase (620 aa), fasta scores: opt: 435, E(): 2.2e-12, (24.8% identity in 545 aa overlap). Weakly similar to Mycobacterium tu [...]

Rv2379c, (MTCY27.01), len: 1461 aa. MbtF, peptide synthetase (see citations below), similar in part to several synthases e.g. O52820|PCZA363.4 protein from Amycolatopsis orientalis (4077 aa), FASTA scores: opt: 1873, E(): 1.1e-99, (35.55% identity in 1522 aa overlap); O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 1817, E(): 2.1e-96, (33.65% identity in 1463 aa overlap); O52821 protein similar to peptide synthetase from Amycolatopsis orientalis (1860 aa) FASTA scores: opt: 1705,E(): 2.9e-90, (34.75% identity in 1344 aa ov [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Rv1683, (MTCI125.05), len: 999 aa. Possible bifunctional long-chain acyl-CoA synthase and lipase. Equivalent to Z95117|MLCB1351_21 possible long-chain acyl-CoA synthase from Mycobacterium leprae (1002 aa) (85.6% identity in 1002 aa overlap). Weakly similar to FATP_MOUSE|Q60714 long-chain fatty acid transport protein (646 aa), fasta scores: opt: 331, E(): 5e-08, (24.8% identity in 630 aa overlap). Also similar to O35488|AF033031 Mouse very-long-chain acyl-CoA synthetase (620 aa), fasta scores: opt: 435, E(): 2.2e-12, (24.8% identity in 545 aa overlap). Weakly similar to Mycobacterium tu [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Possible fatty-acid-CoA ligase FadD1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase); Rv1750c, (MTCY28.13c, MTCY04C12.34), len: 532 aa. Possible fadD1, fatty-acid-CoA synthetase, similar in part to others e.g. O35488|VLCS_MOUSE very-long-chain acyl-CoA synthetase from Mus musculus (620 aa); NP_113924.1|NM_031736 solute carrier family 27 (fatty acid transporter) member 2 from Rattus norvegicus (620 aa); NP_459076.1|NC_003197 crotonobetaine/carnitine-CoA ligase from Salmonella typhimurium (517 aa); CAIC_ECOLI|P31552 probable crotonobetaine/carnitine-CoA ligase from Escherichia coli [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Possible fatty-acid-CoA ligase FadD10 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase); Rv0099, (MTCY251.18), len: 540 aa. Possible fadD10,fatty-acid-CoA synthetase, similar to many. Contains PS00455 putative AMP-binding domain signature. Contains IPR000873 AMP-dependent synthetase/ligase domain. Belongs to the ATP-dependent AMP-binding enzyme family.

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Rv1427c, (MTCY493.27), len: 535 aa. Possible fadD12,long-chain-fatty-acid-CoA synthetase, similar to many e.g. NP_302632.1|NC_002677 acyl-CoA synthase from Mycobacterium leprae (548 aa); AAD01929.2|AF031419 putative long-chain-fatty-acid--CoA ligase from Pseudomonas putida (565 aa); NP_419782.1|NC_002696 putative long-chain-fatty-acid--CoA ligase from Caulobacter crescentus (530 aa); PC60_YEAST|P38137 yeast peroxisomal-coenzyme A synthetase (543 aa), FASTA scores: opt: 507, E(): 2.9e-25, (30.4% identity in 365 aa overlap). Also similar to many M. tuberculosis proteins e.g. MTCY06A4.14 [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Probable chain-fatty-acid-CoA ligase FadD13 (fatty-acyl-CoA synthetase); Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. Catalyzes the activation of long-chain fatty acids as acyl-coenzyme A (acyl-CoA), which are then transferred to the multifunctional polyketide synthase (PKS) type III for further chain extension. It has preference for the fatty acid with long chain length in the following order: hexacosanoic acid (C26), tetracosanoic acid (C24) and palmitic acid (C16); Belongs to the ATP-dependent AMP-bi [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Fatty-acid-CoA ligase FadD14; Rv1058, (MTV017.11), len: 543 aa. Probable fadD14,medium-chain fatty-acid-CoA synthetase, highly similar to many e.g. CAC32346.1|AL583945 putative fatty acid CoA ligase from Streptomyces coelicolor (558 aa); N-terminus of NP_419738.1|NC_002696 medium-chain-fatty-acid--CoA ligase from Caulobacter crescentus (1006 aa); Q00594|ALKK_PSEOL medium-chain-fatty-acid--CoA ligase from Pseudomonas oleovorans (546 aa), FASTA scores: opt: 1468, E(): 0,(41.1% identity in 538 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dep [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Fatty-acid-CoA synthetase FadD17 (fatty-acid-CoA synthase) (fatty-acid-CoA ligase); Catalyzes the activation of long-chain fatty acids as acyl- coenzyme A (acyl-CoA), which are then transferred to the multifunctional polyketide synthase (PKS) type III for further chain extension.

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase); Catalyzes the activation of long-chain fatty acids as acyl- coenzyme A (acyl-CoA), which are then transferred to the multifunctional polyketide synthase (PKS) type III for further chain extension. Also involved in the degradation of cholesterol via the degradation of the side chains of C-24 branched-chain sterols. Catalyzes the ATP-dependent CoA thioesterification of the sterol 3- oxocholest-4-en-26-oate to yield 3-oxocholest-4-en-26-oyl-CoA. It can also use 3beta-hydroxy-5-cholesten-26-oate.

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Rv0270, (MTCY06A4.14), len: 560 aa. Probable fadD2,fatty-acid-CoA synthetase, similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase from Escherichia coli (561 aa), FASTA scores: opt: 544, E(): 2.9e-26, (27.7% identity in 535 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY493_2,MTCY8D5_9, MTCY6G11_8, etc. Contains PS00455 Putative AMP-binding domain signature.

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Probable fatty-acid-AMP ligase FadD21 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Rv1185c, (MTV005.21c), len: 578 aa. Probable fadD21,fatty-acid-AMP synthetase, highly similar to several from Mycobacteria e.g. NP_301895.1|NC_002677 possible acyl-CoA synthase from Mycobacterium leprae (579 aa); P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2388, E(): 0, (61.8% identity in 579 aa overlap); etc. Seems to belong to the ATP-dependent AMP-binding enzyme family. Nucleotide position 1327402 in the genome sequence has been corrected, T:C result [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

P-hydroxybenzoyl-AMP ligase FadD22; Catalyzes the adenylation of p-hydroxybenzoic acid (pHBA) to form p-hydroxybenzoic acid-AMP (pHBA-AMP), which is converted directly to p-hydroxybenzoyl-S-FadD22 (pHBA-S-FAdD22) thioester intermediate in a CoA-independent manner by attack of the phosphopantetheine thiol of FadD22. Usually, this intermediate primes the biosynthesis of the phenolphthiocerol (PPOL) by presenting the pHBA starter unit for elongation by Pks15/1, but M.tuberculosis lacks Pks15/1 due to a natural frameshift and thus is unable to produce PPOL. Belongs to the ATP-dependent AMP [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Probable fatty-acid-AMP ligase FadD23 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Catalyzes the activation of long-chain fatty acids as acyl- coenzyme A (acyl-CoA), which are then transferred to the multifunctional polyketide synthase (PKS) type III for further chain extension (Probable). Involved in the biosynthesis of sulfolipid 1 (SL- 1); Belongs to the ATP-dependent AMP-binding enzyme family.

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Probable fatty-acid-AMP ligase FadD24 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Rv1529, (MTV045.03), len: 584 aa. Probable fadD24,fatty-acid-AMP synthetase, highly similar to many e.g. MBU75685_1|AAB52538.1|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA score: (65.6% identity in 582 aa overlap); and many other fatty-acid-CoA synthetases from Mycobacteria e.g. fadD25|MTCY19G5_7 from Mycobacterium tuberculosis (583 aa), FASTA score: (68.7% identity in 584 aa overlap); fadD28|MTCY24G1_8 from Mycobacterium tuberculosis (580 aa), FASTA score: (66.0% identity [...]

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25,fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc.

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Fatty-acid-AMP ligase FadD26 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Catalyzes the activation of long-chain fatty acids (C22-24 fatty acids) as acyl-adenylates (acyl-AMP), which are then transferred to the multifunctional polyketide synthase PpsA for further chain extension. Involved in the biosynthesis of phthiocerol dimycocerosate (DIM A) and phthiodiolone dimycocerosate (DIM B).

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation; Belongs to the ATP-dependent AMP-binding enzyme family.

Fatty-acid-AMP ligase FadD28 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Catalyzes the activation of long-chain fatty acids (C22-24 fatty acids) as acyl-adenylates (acyl-AMP), which are then transferred to the multifunctional polyketide synthase Mas for further chain extension. Involved in the biosynthesis of mycoserates. Probably plays a role in host phagosome maturation arrest. Belongs to the ATP-dependent AMP-binding enzyme family.