Rv1636, (MTCY01B2.28), len: 146 aa. TB15.3,iron-regulated universal stress protein family protein (see citations below), similar to other hypothetical proteins from diverse organisms e.g. Q57951|MJ0531|Y531_METJA from Methanococcus jannaschii (170 aa), FASTA scores: opt: 188,E(): 6e-06, (32.2% identity in 149 aa overlap); also P42297|YXIE_BACSU hypothetical 15.9 kDa protein in bglh-wapa intergenic region precursor from Bacillus subtilis (148 aa), FASTA scores: opt: 162, E(): 0.00025,(30.8% identity in 156 aa overlap). Part of family of Mycobacterium tuberculosis hypothetical proteins ( [...]

Conserved protein TB16.3; Rv2185c, (MTV021.18c), len: 144 aa. TB16.3,conserved protein, similar to other hypothetical actinomycete proteins and equivalent to Mycobacterium leprae ML0889 (144 aa). Some similarity to Mycobacterium tuberculosis Rv0854, Rv0856, Rv0857, Rv0164 and other Mycobacterium leprae proteins. FASTA scores : ML0889 opt: 811; 85.417% identity in 144 aa overlap (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).

Rv1636, (MTCY01B2.28), len: 146 aa. TB15.3,iron-regulated universal stress protein family protein (see citations below), similar to other hypothetical proteins from diverse organisms e.g. Q57951|MJ0531|Y531_METJA from Methanococcus jannaschii (170 aa), FASTA scores: opt: 188,E(): 6e-06, (32.2% identity in 149 aa overlap); also P42297|YXIE_BACSU hypothetical 15.9 kDa protein in bglh-wapa intergenic region precursor from Bacillus subtilis (148 aa), FASTA scores: opt: 162, E(): 0.00025,(30.8% identity in 156 aa overlap). Part of family of Mycobacterium tuberculosis hypothetical proteins ( [...]

Heat shock protein HspX (alpha-crystallin homolog) (14 kDa antigen) (HSP16.3); Acts as a chaperone, as it has a significant ability to suppress the thermal denaturation of alcohol dehydrogenase. Cells overexpressing this gene grow more slowly than wild-type cells, and are less susceptible to autolysis following saturation of the culture in vitro, suggesting this protein may slow down the growth rate of M.tuberculosis in culture and by extension during macrophage infection. Belongs to the small heat shock protein (HSP20) family.

Rv1636, (MTCY01B2.28), len: 146 aa. TB15.3,iron-regulated universal stress protein family protein (see citations below), similar to other hypothetical proteins from diverse organisms e.g. Q57951|MJ0531|Y531_METJA from Methanococcus jannaschii (170 aa), FASTA scores: opt: 188,E(): 6e-06, (32.2% identity in 149 aa overlap); also P42297|YXIE_BACSU hypothetical 15.9 kDa protein in bglh-wapa intergenic region precursor from Bacillus subtilis (148 aa), FASTA scores: opt: 162, E(): 0.00025,(30.8% identity in 156 aa overlap). Part of family of Mycobacterium tuberculosis hypothetical proteins ( [...]

Probable iron-regulated peptidyl-prolyl cis-trans isomerase A PpiA (PPIase A) (rotamase A); PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides; Belongs to the cyclophilin-type PPIase family.

Rv1636, (MTCY01B2.28), len: 146 aa. TB15.3,iron-regulated universal stress protein family protein (see citations below), similar to other hypothetical proteins from diverse organisms e.g. Q57951|MJ0531|Y531_METJA from Methanococcus jannaschii (170 aa), FASTA scores: opt: 188,E(): 6e-06, (32.2% identity in 149 aa overlap); also P42297|YXIE_BACSU hypothetical 15.9 kDa protein in bglh-wapa intergenic region precursor from Bacillus subtilis (148 aa), FASTA scores: opt: 162, E(): 0.00025,(30.8% identity in 156 aa overlap). Part of family of Mycobacterium tuberculosis hypothetical proteins ( [...]

50S ribosomal protein L7/L12 RplL (SA1); Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation; Belongs to the bacterial ribosomal protein bL12 family.

Rv1636, (MTCY01B2.28), len: 146 aa. TB15.3,iron-regulated universal stress protein family protein (see citations below), similar to other hypothetical proteins from diverse organisms e.g. Q57951|MJ0531|Y531_METJA from Methanococcus jannaschii (170 aa), FASTA scores: opt: 188,E(): 6e-06, (32.2% identity in 149 aa overlap); also P42297|YXIE_BACSU hypothetical 15.9 kDa protein in bglh-wapa intergenic region precursor from Bacillus subtilis (148 aa), FASTA scores: opt: 162, E(): 0.00025,(30.8% identity in 156 aa overlap). Part of family of Mycobacterium tuberculosis hypothetical proteins ( [...]

Probable succinyl-CoA synthetase (beta chain) SucC (SCS-beta); Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.

Conserved protein TB16.3; Rv2185c, (MTV021.18c), len: 144 aa. TB16.3,conserved protein, similar to other hypothetical actinomycete proteins and equivalent to Mycobacterium leprae ML0889 (144 aa). Some similarity to Mycobacterium tuberculosis Rv0854, Rv0856, Rv0857, Rv0164 and other Mycobacterium leprae proteins. FASTA scores : ML0889 opt: 811; 85.417% identity in 144 aa overlap (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).

Rv1636, (MTCY01B2.28), len: 146 aa. TB15.3,iron-regulated universal stress protein family protein (see citations below), similar to other hypothetical proteins from diverse organisms e.g. Q57951|MJ0531|Y531_METJA from Methanococcus jannaschii (170 aa), FASTA scores: opt: 188,E(): 6e-06, (32.2% identity in 149 aa overlap); also P42297|YXIE_BACSU hypothetical 15.9 kDa protein in bglh-wapa intergenic region precursor from Bacillus subtilis (148 aa), FASTA scores: opt: 162, E(): 0.00025,(30.8% identity in 156 aa overlap). Part of family of Mycobacterium tuberculosis hypothetical proteins ( [...]

Conserved protein TB16.3; Rv2185c, (MTV021.18c), len: 144 aa. TB16.3,conserved protein, similar to other hypothetical actinomycete proteins and equivalent to Mycobacterium leprae ML0889 (144 aa). Some similarity to Mycobacterium tuberculosis Rv0854, Rv0856, Rv0857, Rv0164 and other Mycobacterium leprae proteins. FASTA scores : ML0889 opt: 811; 85.417% identity in 144 aa overlap (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).

6 kDa early secretory antigenic target EsxA (ESAT-6); A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL- 12 p40 (IL12B) and TNF-alpha expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protecti [...]

Conserved protein TB16.3; Rv2185c, (MTV021.18c), len: 144 aa. TB16.3,conserved protein, similar to other hypothetical actinomycete proteins and equivalent to Mycobacterium leprae ML0889 (144 aa). Some similarity to Mycobacterium tuberculosis Rv0854, Rv0856, Rv0857, Rv0164 and other Mycobacterium leprae proteins. FASTA scores : ML0889 opt: 811; 85.417% identity in 144 aa overlap (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).

10 kDa culture filtrate antigen EsxB (LHP) (CFP10); A secreted protein. Acts as a strong host (human) T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA; native protein binds poorly to artificial liposomes in the absence or presence of EsxA. EsxA and EsxA-EsxB are cytotoxic to pneumocytes. EsxB (and EsxA-EsxB but not EsxA alone) activates human neutrophils; EsxB transiently induces host (human) intracellular Ca(2+) mobility in a dose-depend [...]

Alanine and proline-rich secreted protein Apa; A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals. Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells. Functions as an adhesin, binds to mouse macrophages via mannose residues. Might interact via host CD209. Belongs to the Apa family.

Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70); Acts as a chaperone; Belongs to the heat shock protein 70 family.

Alanine and proline-rich secreted protein Apa; A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals. Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells. Functions as an adhesin, binds to mouse macrophages via mannose residues. Might interact via host CD209. Belongs to the Apa family.

6 kDa early secretory antigenic target EsxA (ESAT-6); A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL- 12 p40 (IL12B) and TNF-alpha expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protecti [...]

Alanine and proline-rich secreted protein Apa; A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals. Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells. Functions as an adhesin, binds to mouse macrophages via mannose residues. Might interact via host CD209. Belongs to the Apa family.

10 kDa culture filtrate antigen EsxB (LHP) (CFP10); A secreted protein. Acts as a strong host (human) T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA; native protein binds poorly to artificial liposomes in the absence or presence of EsxA. EsxA and EsxA-EsxB are cytotoxic to pneumocytes. EsxB (and EsxA-EsxB but not EsxA alone) activates human neutrophils; EsxB transiently induces host (human) intracellular Ca(2+) mobility in a dose-depend [...]

Alanine and proline-rich secreted protein Apa; A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals. Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells. Functions as an adhesin, binds to mouse macrophages via mannose residues. Might interact via host CD209. Belongs to the Apa family.

60 kDa chaperonin 2; Prevents aggregation of substrate proteins and promotes their refolding.

Alanine and proline-rich secreted protein Apa; A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals. Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells. Functions as an adhesin, binds to mouse macrophages via mannose residues. Might interact via host CD209. Belongs to the Apa family.

Heat shock protein HspX (alpha-crystallin homolog) (14 kDa antigen) (HSP16.3); Acts as a chaperone, as it has a significant ability to suppress the thermal denaturation of alcohol dehydrogenase. Cells overexpressing this gene grow more slowly than wild-type cells, and are less susceptible to autolysis following saturation of the culture in vitro, suggesting this protein may slow down the growth rate of M.tuberculosis in culture and by extension during macrophage infection. Belongs to the small heat shock protein (HSP20) family.

Alanine and proline-rich secreted protein Apa; A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals. Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells. Functions as an adhesin, binds to mouse macrophages via mannose residues. Might interact via host CD209. Belongs to the Apa family.

Immunogenic protein Mpt63 (antigen Mpt63/MPB63) (16 kDa immunoprotective extracellular protein); Rv1926c, (MT1977, MTCY09F9.38), len: 159 aa. Mpt63 (alternate gene name: mpb63), immunogenic protein (see citations below), identical to MPT63|MPB63 from Mycobacterium bovis (159 aa). Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al., 2008).

Alanine and proline-rich secreted protein Apa; A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals. Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells. Functions as an adhesin, binds to mouse macrophages via mannose residues. Might interact via host CD209. Belongs to the Apa family.

Immunogenic protein Mpt64 (antigen Mpt64/MPB64); Rv1980c, (MT2032, MTCY39.39), len: 228 aa. Mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below),identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al.,2008).

Alanine and proline-rich secreted protein Apa; A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals. Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells. Functions as an adhesin, binds to mouse macrophages via mannose residues. Might interact via host CD209. Belongs to the Apa family.

Cell surface lipoprotein Mpt83 (lipoprotein P23); Recombinant, non-modified protein stimulates secretion of cytokines (TNF-alpha, IL-6 and IL-12p40) by mouse macrophage cell lines in a TLR2-dependent fashion, which leads to increased host innate immunity responses against the bacterium. Serves as a strong human and mouse antigen T cell antigen during M.tuberculosis infection, inducing strong IFN-gamma expression.

Probable bacterioferritin BfrA; Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.

Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70); Acts as a chaperone; Belongs to the heat shock protein 70 family.

Probable bacterioferritin BfrA; Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.

Conserved lipoprotein LprG; Probably helps membrane protein Rv1410c (P55) transport triacylglycerides (TAG) across the inner cell membrane into the periplasm; TAG probably regulates lipid metabolism and growth regulation. Binds TAG and transfers it between lipid bilayers, probably to the outer membrane in vivo. Binds di- and triacylated phosphatidyl-myo-inositol mannosides (PIMs), and glycolipid lipoglycan modulins lipoarabinomannan (LAM) and lipomannan (LM), facilitating their recognition by TLR2. Binds LM > PIM6 > ManLAM > PI-LAM > PIM2 (mannose-capped LAM and phospho-myo-inositol-ca [...]

Probable bacterioferritin BfrA; Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.

Immunogenic protein Mpt64 (antigen Mpt64/MPB64); Rv1980c, (MT2032, MTCY39.39), len: 228 aa. Mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below),identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al.,2008).

Probable bacterioferritin BfrA; Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.

Probable iron-regulated elongation factor TU Tuf (EF-TU); This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis.