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| | vapC29 | Possible toxin VapC29. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. Its cognate antitoxin is VapB29 (By similarity). Has ribonuclease activity. (133 aa) |
| | vapB27 | Possible antitoxin VapB27; Antitoxin component of a type II toxin-antitoxin (TA) system. Cognate toxin is VapC27. Upon expression in E.coli partially counteracts the ribonuclease activity of non-cognate toxins MazF6 and MazF9; Belongs to the VapB family. (78 aa) |
| | vapC27 | Possible toxin VapC27. Contains PIN domain; Probably the toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Its cognate antitoxin is VapB27. Belongs to the PINc/VapC protein family. (137 aa) |
| | mazE1 | Possible antitoxin MazE1; Antitoxin component of a type II toxin-antitoxin (TA) system. (57 aa) |
| | mazF1 | Possible toxin MazF1; Toxic component of a type II toxin-antitoxin (TA) system, its cognate antitoxin is MazE1 (Probable). Probably an endoribonuclease (By similarity); Belongs to the PemK/MazF family. (93 aa) |
| | dnaK | Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70); Acts as a chaperone; Belongs to the heat shock protein 70 family. (625 aa) |
| | vapC1 | Possible toxin VapC1; Toxic component of a type II toxin-antitoxin (TA) system. The cognate antitoxin is VapB1 (By similarity). Has ribonuclease activity. (133 aa) |
| | Rv0059 | Hypothetical protein; Rv0059, (MTV030.02), len: 230 aa. Hypothetical unknown protein. (230 aa) |
| | Rv3662c | Rv3662c, (MTV025.010c), len: 256 aa. Conserved hypothetical protein, equivalent to Q9CB99|ML2289 hypothetical protein from Mycobacterium leprae (256 aa) FASTA scores: opt: 1255, E(): 3.3e-69, (78.05% identity in 255 aa overlap). Also similar to Q9X924|SCH5.22c putative oxidoreductase from Streptomyces coelicolor (274 aa), FASTA scores: opt: 289, E(): 1.8e-10, (39.25% identity in 270 aa overlap). (256 aa) |
| | topA | DNA topoisomerase 1; Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA super [...] (934 aa) |
| | clpC1 | Probable ATP-dependent protease ATP-binding subunit ClpC1; ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP (By similarity). Degrades anti-sigma-E factor RseA in the presence of ClpP2. (848 aa) |
| | echA20 | Probable enoyl-CoA hydratase EchA20 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv3550, (MTCY03C7.06c), len: 247 aa. Probable echA20, enoyl-CoA hydratase, similar to others e.g. Q9A7B0|CC1814 from Caulobacter crescentus (275 aa), FASTA scores: opt: 488, E(): 3.5e-24, (36.4% identity in 239 aa overlap); O84978|PHAA from Pseudomonas putida (293 aa),FASTA scores: opt: 383, E(): 2e-17, (33.85% identity in 254 aa overlap); BAB48479|Q98LI4|MLL1009 from Rhizobium loti (Mesorhizobium loti) (258 aa), FASTA scores: opt: 378, E(): 3.8e-17, (21.45% identity in 231 aa overlap); et [...] (247 aa) |
| | fadE28 | Probable acyl-CoA dehydrogenase FadE28; Involved in the third cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. May play an important role for the initial macrophage invasion, possibly in response to the acidification of phagosome. It contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the dehydrogenation of 2'-propanoyl-CoA ester side chains of 3-oxo-4- pregnene-20-carboxyl-CoA (3-OPC-CoA) to yield 3-oxo-4,17-pregnadiene- 20-carboxyl-CoA (3-OPDC-CoA). Also able to dehydrogenate steroyl-CoA such as 3-oxo-chol- [...] (339 aa) |
| | fadE29 | Probable acyl-CoA dehydrogenase FadE29; Involved in the third cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. Contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the dehydrogenation of 2'-propanoyl-CoA ester side chains of 3-oxo-4- pregnene-20-carboxyl-CoA (3-OPC-CoA) to yield 3-oxo-4,17-pregnadiene- 20-carboxyl-CoA (3-OPDC-CoA). Also able to dehydrogenate steroyl-CoA such as 3-oxo-chol-4-en-24-oyl-CoA (3-OCO-CoA), 1beta-(2'-propanoyl- CoA)-3a-alpha-H- 7a-beta-methylhexahydro-4-indanone (indanone-CoA ester), [...] (387 aa) |
| | relK | Toxin RelK; Toxic component of a type II toxin-antitoxin (TA) system. Has RNase activity and preferentially cleaves at the 3'-end of purine ribonucleotides (By similarity). Overexpression in M.tuberculosis or M.smegmatis inhibits colony formation in a bacteriostatic rather than bacteriocidal fashion. Its toxic effect is neutralized by coexpression with antitoxin RelJ (shown only for M.smegmatis). Overexpression also increases the number of rifampcin-tolerant persister cells. Belongs to the YoeB family. (85 aa) |
| | relJ | Antitoxin RelJ; Antitoxin component of a type II toxin-antitoxin (TA) system. A probable antitoxin for the putative mRNA interferase RelK. Upon expression in E.coli but not in M.smegmatis this protein neutralizes E.coli YoeB. (91 aa) |
| | vapC44 | Possible toxin VapC44. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB44 (By similarity). (142 aa) |
| | rubB | Probable rubredoxin RubB; Rv3250c, (MTCY20B11.25c), len: 60 aa. Probable rubB,rubredoxin, highly similar to other rubredoxins e.g. Q9AE66|RUBA4 from Rhodococcus erythropolis (60 aa), FASTA scores: opt: 391, E(): 2.2e-21, (83.05% identity in 59 aa overlap); Q9AE63|RUBA2 from Rhodococcus erythropolis (63 aa), FASTA scores: opt: 380, E(): 1.4e-20, (83.9% identity in 56 aa overlap); P42453|RUBR_ACICA|RUBA from Acinetobacter calcoaceticus (54 aa), FASTA scores: opt: 315, E(): 4.9e-16, (69.8% identity in 53 aa overlap); Q9HTK7|PA5351 from Pseudomonas aeruginosa (55 aa), FASTA scores: opt: 29 [...] (60 aa) |
| | secA1 | Probable preprotein translocase SecA1 1 subunit; Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of precursor proteins, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane (By similarity). (949 aa) |
| | whiB7 | Probable transcriptional regulatory protein WhiB-like WhiB7; The apo- but not holo-form probably binds DNA (By similarity). Acts as a transcriptional regulator. Probably redox- responsive. Upon overproduction at least 10 other genes are up- regulated, among them are Rv1258c, Rv1988, Rv2301, Rv2416c, Rv2725c and whiB7 itself. Probably redox-responsive. The apo-form has been shown to act as a protein disulfide reductase. (92 aa) |
| | Rv3189 | Conserved hypothetical protein; Probable toxic component of a type II toxin-antitoxin (TA) system. Degrades NAD(+) by phosphorolysis. Neutralized by its cognate antitoxin Rv3188. (206 aa) |
| | higA3 | Possible transcriptional regulatory protein; Putative antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin would be HigB3. (109 aa) |
| | higB3 | Conserved hypothetical protein; Putative toxic component of a type II toxin-antitoxin (TA) system. Its cognate antitoxin would be HigA3. Not toxic upon expression in M.smegmatis. (114 aa) |
| | Rv3173c | Rv3173c, (MTV014.17c), len: 200 aa. Probable transcriptional regulatory protein TetR family, similar to several bacterial putative regulatory proteins e.g. Q9EWI2|SC7H9.14 from Streptomyces coelicolor (195 aa),FASTA scores: opt: 319, E(): 1.7e-13, (34.55% identity in 195 aa overlap); O85695|3SCF60.04 from Streptomyces lividans and Streptomyces coelicolor (192 aa), FASTA scores: opt: 297, E(): 4.3e-12, (37.45% identity in 187 aa overlap); BAB50853|MLR4117 from Rhizobium loti (Mesorhizobium loti) (205 aa), FASTA scores: opt: 280, E(): 5.5e-11, (31.45% identity in 194 aa overlap); BAB5376 [...] (200 aa) |
| | relG | Toxin RelG; Toxic component of a type II toxin-antitoxin (TA) system. Has RNase activity and preferentially cleaves at the 3'-end of purine ribonucleotides (By similarity). Overexpression in M.tuberculosis or M.smegmatis inhibits colony formation in a bacteriostatic rather than bacteriocidal fashion. Its toxic effect is neutralized by coexpression with cognate antitoxin RelB2 (shown only for M.smegmatis). Overexpression also increases the number of gentamicin-tolerant and levofloxacin-tolerant persister cells. (87 aa) |
| | relF | Antitoxin RelF; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in M.smegmatis neutralizes the effect of toxin RelE2. (93 aa) |
| | vapC22 | Possible toxin VapC22; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits translation and colony formation. Its toxic effect on colony formation is neutralized by coexpression with cognate antitoxin VapB22; the effect on translation has not been tested but is probably neutralized also. (130 aa) |
| | Rv2827c | Hypothetical protein; Rv2827c, (MTCY16B7.15), len: 295 aa. Hypothetical unknown protein, equivalent to AAK47219 from Mycobacterium tuberculosis strain CDC1551 (315 aa) but shorter 20 aa. (295 aa) |
| | Rv2826c | Hypothetical protein; Rv2826c, (MTCY16B7.16), len: 294 aa. Hypothetical unknown protein. (294 aa) |
| | mazF9 | Toxin MazF9; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE9. Acts as an mRNA interferase, specifically cleaving between U and C in UAC sequences. May cleave its cognate antitoxin's gene. In E.coli expression with non- cognate antitoxins VapB27 and VapB40 partially neutralizes the toxin. Belongs to the PemK/MazF family. (118 aa) |
| | recA | RecA protein (recombinase A) [contains: endonuclease PI-MTUI (MTU RecA intein)]; Can catalyze the hydrolysis of ATP in the presence of single- stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage. (790 aa) |
| | lexA | Repressor LexA; Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Has been shown to bind to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (236 aa) |
| | ideR | Iron-dependent repressor and activator IdeR; Metal-dependent DNA-binding protein that controls transcription of many genes involved in iron metabolism. Acts as a repressor of siderophore biosynthesis and as a positive modulator of iron storage. Also regulates expression of transporters, proteins involved in siderophore synthesis, iron storage and transcriptional regulators. (230 aa) |
| | clpC2 | Rv2667, (MTCY441.36), len: 252 aa. Possible clpC2,ATP-dependent protease atp-binding subunit, highly similar to Q9X8L2|SCE9.40 hypothetical 27.3 KDA protein from Streptomyces coelicolor (258 aa), FASTA scores: opt: 877,E(): 2.2e-46, (57.25% identity in 255 aa overlap). The second half of the protein is highly similar to N-terminal of several CLP-family proteins e.g. P24428|CLPC_MYCLE|ML0235 probable ATP-dependent CLP protease ATP-binding subunit from Mycobacterium leprae (848 aa), FASTA scores: opt: 307, E(): 3.2e-11, (38.6% identity in 158 aa overlap); O06286|CLPC_MYCTU|Rv3596c|MT3703 [...] (252 aa) |
| | Rv2654c | Possible PhiRv2 prophage protein; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in M.smegmatis neutralizes the effect of cognate toxin Rv2653c. (81 aa) |
| | vapB40 | Possible antitoxin VapB40; Antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin is VapC40. Upon expression in E.coli partially counteracts the ribonuclease activity of non-cognate toxins MazF6 and MazF9; Belongs to the VapB family. (81 aa) |
| | relA | Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase; In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the formation of pppGpp, which is then hydrolyzed to form ppGpp, as well as the hydrolysis of ppGpp. RelA is probably a key factor in the pathogenesis of M.tuberculosis as it regulates the intracellular concentrations of (p)ppGpp. (790 aa) |
| | vapC20 | Possible toxin VapC20; Toxic component of a type II toxin-antitoxin (TA) system. An endoribonuclease that cleaves both E.coli and M.smegmatis 23S rRNA between G2661 and A2662 in the sarcin-ricin loop (SRL, E.coli 23S rRNA numbering). The SRL sequence is highly conserved and is implicated in GTP hydrolysis by EF-Tu and EF-G. Acts on purified ribosomes but not on isolated RNA in E.coli, nor on a shortened artificial substrate. Upon expression in E.coli inhibits cell growth, colony formation and translation. Its toxic effect is neutralized by coexpression, or subsequent expression (tested [...] (131 aa) |
| | vapC19 | Possible toxin VapC19; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB19; Belongs to the PINc/VapC protein family. (125 aa) |
| | vapC39 | Possible toxin VapC39. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB39. (139 aa) |
| | vapC17 | Possible toxin VapC17; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB17 (By similarity). Belongs to the PINc/VapC protein family. (133 aa) |
| | vapC38 | Possible toxin VapC38. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB38 (By similarity). (141 aa) |
| | tig | Probable trigger factor (TF) protein Tig; Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase (By similarity). Belongs to the FKBP-type PPIase family. Tig subfamily. (466 aa) |
| | clpP1 | Probable ATP-dependent CLP protease proteolytic subunit 1 ClpP1 (endopeptidase CLP); Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). Degrades anti-sigma-D factor (rsdA) when present in a complex with ClpP2 and ClpX. Does not seem to act on anti-sigma-L factor (rslA). Belongs to the peptidase S14 family. (200 aa) |
| | zur | Probable zinc uptake regulation protein Zur; Global transcriptional regulator involved in zinc homeostasis. Represses the transcription of at least 32 genes, including genes involved in zinc homeostasis, by binding to promoter sequences that contain a conserved 26 bp palindrome, in the presence of zinc; Belongs to the Fur family. (130 aa) |
| | parE2 | Possible toxin ParE2; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin ParD2. (105 aa) |
| | parD2 | Possible antitoxin ParD2; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli neutralizes the effect of cognate toxin ParE2. (71 aa) |
| | vapC37 | Possible toxin VapC37. Contains PIN domain; Probable toxic component of a type II toxin-antitoxin (TA) system. An RNase. Upon expression in M.smegmatis inhibits colony formation. The putative cognate antitoxin is VapB37. (144 aa) |
| | Rv2035 | Rv2035, (MTV018.22), len: 162 aa. Conserved hypothetical protein, similar to many. Contains IPR013538 Activator of Hsp90 ATPase homologue 1-like. (162 aa) |
| | Rv2034 | ArsR repressor protein; Involved in the regulation of lipid metabolism and hypoxic response. Positively regulates transcription of various genes, such as phoP, groEL2 and dosR. Negatively regulates its own transcription. Acts by binding to a specific palindromic sequence motif in promoter regions. (107 aa) |
| | higB2 | Conserved protein; Putative toxic component of a type II toxin-antitoxin (TA) system. Its cognate antitoxin would be HigA2. Belongs to the mycobacterial HigB family. (201 aa) |
| | higA2 | Transcriptional regulatory protein; Putative antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin would be HigB2. (101 aa) |
| | mazF6 | Toxin MazF6; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis partially inhibits cell growth and colony formation; its toxic effect is neutralized by coexpression with cognate antitoxin MazE6. Acts as an mRNA interferase on ssRNA, cleaving between the second and third bases in the sequences CUCCU and UUCCU. Further experiments demonstrate that it digests between the first and second bases of UCCUU, yielding a 5'- hydroxyl end; digests M.tuberculosis mRNA (in coding as well as the 5'- and 3'-UTR regions) and 23S rRNA, digests E.coli [...] (114 aa) |
| | mazE6 | Antitoxin MazE6; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis counteracts the ribonuclease activity of cognate toxin MazF6. (82 aa) |
| | mbcT | Hypothetical protein; Toxic component of a type II toxin-antitoxin (TA) system. Neutralized by cognate antitoxin MbcA. Degrades NAD(+) by phosphorolysis. Expression in the absence of its cognate antitoxin MbcA causes dramatic reduction of intracellular NAD(+) levels and is deleterious to cell growth, causing cell death. In a SCID mouse infection model, mice infected with bacteria overexpressing this protein survive longer. Overexpression of this protein in a mouse infection model at 21 days leads to bacterial death, and shows a synergistic 100-fold increase in mouse survival when combi [...] (186 aa) |
| | parD1 | Possible antitoxin ParD1; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli neutralizes the effect of cognate toxin ParE1. (83 aa) |
| | secBL | Hypothetical protein; Chaperone component of an atypical, type II toxin-antitoxin chaperone (TAC) system. Prevents antitoxin HigA1 aggregation in vitro at a 1:3 chaperone:antitoxin ratio, probably also protects antitoxin HigA1 from protease. Required for neutralization of toxin HigB1 upon ectopic expression in Mycobacterium marinum or E.coli. When expressed in E.coli complements a secB deletion, restores export of OmpA and MBP and inhibits aggregation of proOmpC although it is less efficient than endogenous SecB. Complements the general chaperone function of E.coli SecB less well; Belo [...] (181 aa) |
| | higA | Possible antitoxin HigA; Antitoxin component of an atypical, type II toxin-antitoxin chaperone (TAC) system. Upon expression in M.smegmatis neutralizes the effect of cognate toxin HigB1. Neutralization of HigB1 toxin in E.coli or M.marinum also requires SecB-like chaperone Rv1957, making this the first toxin-antitoxin chaperone (TAC) system. Antitoxin aggregation and degradation are prevented by the chaperone. (149 aa) |
| | higB | Possible toxin HigB; Toxic component of an atypical, type II toxin-antitoxin chaperone (TAC) system. Upon expression in M.smegmatis inhibits colony formation and cell growth. Ectopic expression in wild-type M.tuberculosis has no effect on cell growth; ectopic expression in a triple higB1-higA1-Rv1957 (delta TAC) disruption mutant causes growth arrest, killing a considerable proportion of the cells. Increased ectopic expression leads to decreased levels of IdeR- and Zur-regulated genes as well as cleavage within the mRNA region of tmRNA (transfer-mRNA), strongly suggesting it is an endo [...] (125 aa) |
| | Rv1954A | Rv1954A, len: 100 aa. Hypothetical unknown protein. (100 aa) |
| | mazF5 | Possible toxin MazF5; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE5. Probably an endoribonuclease (By similarity). (109 aa) |
| | vapC13 | Possible toxin VapC13; Toxic component of a type II toxin-antitoxin (TA) system. An RNase. The cognate antitoxin is VapB13 (By similarity). (131 aa) |
| | vapC11 | Possible toxin VapC11; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an RNase. Upon expression in E.coli and M.smegmatis inhibits translation, cell growth and colony formation. Its toxic effects on cell growth and colony formation are neutralized by coexpression with cognate antitoxin VapB11; the effect on translation has not been tested but is probably also neutralized. (134 aa) |
| | Rv1546 | Conserved protein; Rv1546, (MTCY48.19c), len: 143 aa. Conserved protein, similar to O05902|Rv0910|MTCY21C12.04 Hypothetical protein from Mycobacterium tuberculosis (144 aa), FASTA scores: E(): 5e-30, (37.3% identity in 142 aa overlap). (143 aa) |
| | Rv1545 | Hypothetical protein; Rv1545, (MTCY48.20), len: 75 aa. Hypothetical unknown protein. (75 aa) |
| | mazF4 | Possible toxin MazF4; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an endoribonuclease (mRNA interferase) on single-strand mRNA, cleaving between the first and second bases in the sequence UCGCU. Overexpression in M.smegmatis but not E.coli inhibits growth, this effect is neutralized by coexpression with cognate toxin MazE4. Belongs to the PemK/MazF family. (105 aa) |
| | relB | Antitoxin RelB; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in M.smegmatis neutralizes the effect of toxin RelE. (89 aa) |
| | relE | Toxin RelE; Toxic component of a type II toxin-antitoxin (TA) system. Has RNase activity (By similarity). Overexpression in M.tuberculosis or M.smegmatis inhibits colony formation in a bacteriostatic rather than bacteriocidal fashion. Its toxic effect is neutralized by coexpression with cognate antitoxin RelB (shown only for M.smegmatis). (97 aa) |
| | mazF3 | Toxin MazF3; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an mRNA and 23S rRNA interferase, cleaving predominantly after the first 2 Us in the sequence 5'-UUCCU-3'; in 23S rRNA only cleaves once in the ribosomal A site in dissociated but not intact ribosomes. Cleavage of 23S rRNA inhibits protein translation; the 23S rRNA region cleaved is involved in tRNA-binding in the A site, 30S and 50S subunit interaction and ribosome recycling factor association. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. It dramatically increases per [...] (103 aa) |
| | Rv1045 | Hypothetical protein; Rv1045, (MTCY10G2.04c), len: 293 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007). (293 aa) |
| | Rv1044 | Rv1044, (MTCY10G2.05c), len: 207 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical protein MTCY06G11.02C|P96837 (289 aa), fasta scores: E(): 8.9e-06, (30.7% identity in 150 aa overlap). Some similarity to U36837|LLU36837_1 Lactococcus lactis plasmid pNP40 (287 aa), FASTA scores: opt: 147, E (): 0.0087, (29.7% identity in 91 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (207 aa) |
| | Rv0998 | Conserved hypothetical protein; Catalyzes specifically the acetylation of the epsilon-amino group of a highly conserved lysine residue in acetyl-CoA synthetase (ACS). This acetylation results in the inactivation of ACS activity and could be important for mycobacteria to adjust to environmental changes. (333 aa) |
| | Rv0918 | Conserved protein; Rv0918, (MTCY21C12.12), len: 158 aa. Conserved protein, similar in part to Q50116 hypothetical protein from Mycobacterium leprae (44 aa), FASTA scores: opt: 132,E(): 0.0055, (65.6% identity in 32 aa overlap). Also some similarity in C-terminus with other hypothetical proteins e.g. NP_289961.1|NC_002655 hypothetical protein from Escherichia coli strain O157:H7 (94 aa); etc. (158 aa) |
| | Rv0910 | Conserved hypothetical protein; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin Rv0909. Does not exert its toxic effect via translation. (144 aa) |
| | Rv0909 | Conserved hypothetical protein; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in M.smegmatis neutralizes the effect of cognate toxin Rv0910. (59 aa) |
| | fadB | Rv0860, (MTV043.53), len: 720 aa. Probable fadB,fatty oxidation protein, equivalent to NP_302422.1|NC_002677 putative fatty oxidation complex alpha subunit from Mycobacterium leprae (714 aa). Also highly similar to others and various proteins involved in fatty acid metabolism, e.g. T35429 probable fatty oxidation protein from Streptomyces coelicolor (733 aa); NP_250428.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (714 aa); NP_418895.1|NC_002696 fatty oxidation complex alpha subunit from Caulobacter crescentus (709 aa); P40939|ECHA_HUMAN trifunctional [...] (720 aa) |
| | Rv0837c | Hypothetical protein; Rv0837c, (MTV043.30c), len: 342 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007). (342 aa) |
| | Rv0836c | Hypothetical protein; Rv0836c, (MTV043.29c), len: 217 aa (start uncertain). Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (217 aa) |
| | vapC31 | Possible toxin VapC31. Contains PIN domain; Toxic component of a type II toxin-antitoxin (TA) system. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB31 (By similarity). (142 aa) |
| | mazF2 | Toxin MazF2; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli partially inhibits cell growth after one cell generation. Its cognate antitoxin is MazE2. Probably an endoribonuclease (By similarity); Belongs to the PemK/MazF family. (102 aa) |
