STRINGSTRING
mmaA1 mmaA1 mmpL3 mmpL3 Rv0221 Rv0221 pcaA pcaA cmaA2 cmaA2 echA3 echA3 mmaA4 mmaA4 mmaA3 mmaA3 mmaA2 mmaA2 echA6 echA6 pstB pstB esxK esxK Rv1217c Rv1217c Rv1218c Rv1218c tap tap atpB atpB atpH atpH atpG atpG ribG ribG Rv1458c Rv1458c bioB bioB bacA bacA katG katG Rv2285 Rv2285 folC folC ribD ribD hsdS.1 hsdS.1 hsdS hsdS dfrA dfrA thyA thyA Rv2765 Rv2765 drrA drrA drrB drrB Rv3087 Rv3087 tgs1 tgs1 Rv3253c Rv3253c Rv3254 Rv3254 glpD2 glpD2 lpdA lpdA Rv3304 Rv3304 amiA1 amiA1 amiB1 amiB1 cmaA1 cmaA1 hsaF hsaF folP1 folP1 Rv3720 Rv3720
Nodes:
Network nodes represent proteins
splice isoforms or post-translational modifications are collapsed, i.e. each node represents all the proteins produced by a single, protein-coding gene locus.
Node Color
colored nodes:
query proteins and first shell of interactors
white nodes:
second shell of interactors
Node Content
empty nodes:
proteins of unknown 3D structure
filled nodes:
a 3D structure is known or predicted
Edges:
Edges represent protein-protein associations
associations are meant to be specific and meaningful, i.e. proteins jointly contribute to a shared function; this does not necessarily mean they are physically binding to each other.
Known Interactions
from curated databases
experimentally determined
Predicted Interactions
gene neighborhood
gene fusions
gene co-occurrence
Others
textmining
co-expression
protein homology
Your Input:
mmaA1Mycolic acid methyltransferase MmaA1; Involved in the conversion of a cis-olefin into a trans- olefin with concomitant introduction of an allylic methyl branch at the proximal position of the precursor to both the methoxy and ketomycolic acids. It directly affects the cis- to trans ratio and indirectly affects the keto to methoxy ratio; Belongs to the CFA/CMAS family. (286 aa)
mmpL3Possible conserved transmembrane transport protein MmpL3; Transports trehalose monomycolate (TMM) across the inner membrane. Could also be part of a heme-iron acquisition system. Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily. (944 aa)
Rv0221Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (469 aa)
pcaAMycolic acid synthase PcaA (cyclopropane synthase); Involved in the phagosome maturation block (PMB). Catalyzes the conversion of a double bond to a cyclopropane ring at the proximal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. It can use cis, cis 11,14-eicosadienoic acid and linoelaidic acid as substrate. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence. (287 aa)
cmaA2Cyclopropane mycolic acid synthase 2; Catalyzes the formation of trans cyclopropanated ketomycolate or methoxymycolate through the conversion of a double bond to a cyclopropane ring at the proximal position of an oxygenated mycolic acid via the transfer of a methylene group from S-adenosyl-L- methionine. In the absence of MmaA2, CmaA2 has a non-specific cis- cyclopropanating activity and is able to catalyze the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid. Cyclopropanated mycolic acids are key factors participating in cell envel [...] (302 aa)
echA3Probable enoyl-CoA hydratase EchA3 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv0632c, (MTCY20H10.13c), len: 231 aa. Probable echA3, enoyl-CoA hydratase, almost identical to the MTU88877_1 enoyl-CoA hydratase of Mycobacterium tuberculosis field isolate NTI64719, FASTA score: (92.4% identity in 184 aa overlap). Also similar to others e.g. P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores: opt: 206, E(): 6.3e-07, (31.5% identity in 232 aa overlap); etc. (231 aa)
mmaA4Hydroxymycolate synthase MmaA4; Involved in the biosynthesis of hydroxymycolate, a common precursor of oxygenated mycolic acids (methoxy-mycolate and keto- mycolate). Probably transfers a methyl group from the S- adenosylmethionine (SAM) cofactor and, subsequently or simultaneously, a water molecule onto the double bound of ethylene substrates, leading to the formation of the hydroxylated product at the distal position. Involved in the activation of the antitubercular drug thiacetazone (TAC). (301 aa)
mmaA3Methoxy mycolic acid synthase MmaA3; Involved in the biosynthesis of methoxymycolic acid. It catalyzes the O-methylation of the hydroxy group of the hydroxymycolate to form a methyl ether; Belongs to the CFA/CMAS family. (293 aa)
mmaA2Cyclopropane mycolic acid synthase MmaA2; Catalyzes the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. MmaA2 also catalyzes the biosynthesis of the cis-cyclopropanated methoxymycolates. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence. (287 aa)
echA6Possible enoyl-CoA hydratase EchA6 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Could possibly oxidize fatty acids using specific components. (243 aa)
pstBPhosphate-transport ATP-binding protein ABC transporter PstB; Part of the ABC transporter complex PstSACB involved in phosphate import (Probable). Responsible for energy coupling to the transport system. (276 aa)
esxKRv1197, (MT1235, MTCI364.09), len: 98 aa. EsxK,ESAT-6 like protein (see citation below). Member of M. tuberculosis hypothetical QILSS protein family with Rv1038c, etc. Almost identical to MTCY98.023c (98 aa) (99.0% identity in 98 aa overlap) and MTCY10G2.11 (98 aa),FASTA scores: opt: 643, E(): 0, (99.0% identity in 98 aa overlap); highly similar to Q49945|U1756C from Mycobacterium leprae (100 aa), FASTA scores: opt: 377, E(): 8e-21, (58.3% identity in 96 aa overlap). Belongs to the ESAT6 family. (98 aa)
Rv1217cProbable tetronasin-transport integral membrane protein ABC transporter; Probably part of the ABC transporter complex Rv1217c-Rv1218c involved in the resistance to a wide range of structurally unrelated drugs. Probably responsible for the translocation of the substrate across the membrane (Probable). (548 aa)
Rv1218cProbable tetronasin-transport ATP-binding protein ABC transporter; Probably part of the ABC transporter complex Rv1217c-Rv1218c involved in the resistance to a wide range of structurally unrelated drugs. Could be involved in the efflux of substrates belonging to the diverse chemical classes of novobiocins, biarylpiperazines, pyridines, bisanilinopyrimidines, pyrroles and, to a smaller extent, pyrazolones. Probably responsible for energy coupling to the transport system (Probable). (311 aa)
tapProbable conserved integral membrane transport protein; Efflux pump that contributes to intrinsic antibiotic resistance. The pump uses the electrochemical gradient as a source of energy (By similarity). Confers resistance to rifampicin. Confers low-level resistance to tetracycline and to several aminoglycosides, including streptomycin, gentamicin, 2'-N- ethylnetilmicin and 6'-N-ethylnetilmicin. (419 aa)
atpBProbable ATP synthase a chain AtpB (protein 6); Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane. Belongs to the ATPase A chain family. (250 aa)
atpHProbable ATP synthase delta chain AtpH; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (By similarity). In the C-terminal section; belongs to the ATPase delta chain family. (446 aa)
atpGProbable ATP synthase gamma chain AtpG; Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. (305 aa)
ribGDiaminohydroxyphosphoribosylaminopyrimidine deaminase; Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'- phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'- phosphate; In the C-terminal section; belongs to the HTP reductase family. (339 aa)
Rv1458cRv1458c, (MTV007.05c), len: 313 aa. Possible unidentified antibiotic-transport ATP-binding protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.31 from Mycobacterium leprae (315 aa), FASTA scores: opt: 1812, E(): 0, (88.0% identity in 308 aa overlap). Similar to AF027770|AF027770_7 ABC-type transporter in FxbA region in Mycobacterium smegmatis (284 aa), FASTA scores: opt: 1412, E(): 0, (85.1% identity in 248 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protei [...] (313 aa)
bioBProbable biotin synthetase BioB; Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism. (349 aa)
bacAProbable drug-transport transmembrane ATP-binding protein ABC transporter BacA; ABC transporter involved in uptake of vitamin B12 and related corrinoids. Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation. Contributes to maintenance of chronic infections. (639 aa)
katGCatalase-peroxidase-peroxynitritase T KatG; Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity, oxidizing various electron donors including NADP(H). Protects M.tuberculosis against toxic reactive oxygen species (ROS) including hydrogen peroxide as well as organic peroxides and thus contributes to its survival within host macrophages by countering the phagocyte oxidative burst. Also displays efficient peroxynitritase activity, which may help the bacterium to persist in macrophages. Catalyzes the oxidative activation of the antitubercular pro- drug isoniazid (I [...] (740 aa)
Rv2285Possible triacylglycerol synthase (diacylglycerol acyltransferase); Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). (445 aa)
folCProbable folylpolyglutamate synthase protein FolC (folylpoly-gamma-glutamate synthetase) (FPGS); Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate, leading to folylpolyglutamate derivatives (By similarity). (487 aa)
ribDRv2671, (MTCY441.40), len: 258 aa. Possible ribD (alternate gene name: ribG), bifunctional riboflavin biosynthesis protein incuding diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino) uracil reductase, highly similar to O05684|MLC1351.23|ML1340 possible reductase from Mycobacterium leprae (268 aa), FASTA scores: opt: 1211,E(): 3e-68, (72.9% identity in 251 aa overlap). Also weakly similar to others e.g. Q9HWX2|RIBD|PA4056 riboflavin-specific deaminase/reductase from Pseudomonas aeruginosa (373 aa), FASTA scores: opt: 211, E(): 6.3e-06,(30.1% iden [...] (258 aa)
hsdS.1Rv2755c, (MTV002.20c), len: 91 aa. Possible hsdS.1,fragment of type I restriction/modification system specificity determinant (S protein), similar to the N-terminus of other hsdS proteins e.g. O34140|HSDS from Klebsiella pneumoniae (439 aa), FASTA scores: opt: 303,E(): 2.1e-13, (46.65% identity in 90 aa overlap); P72419|sty|SBLI from Salmonella typhimurium (434 aa), FASTA scores: opt: 278, E(): 1.1e-11, (47.65% identity in 86 aa overlap); and Q9P9X9|XF2741 from Xylella fastidiosa (412 aa), FASTA scores: opt: 144, E(): 0.015, (31.7% identity in 82 aa overlap). Also some similarity with [...] (91 aa)
hsdSRv2761c, (MTV002.26c), len: 364 aa. Possible hsdS,type I restriction/modification system specificity determinant (S protein), similar in part to other hsdS protein (S proteins) e.g. Q9P9X9|XF2741 from Xylella fastidiosa (412 aa), FASTA scores: opt: 252, E(): 7.4e-09,(24.95% identity in 401 aa overlap); N-terminus of Q9RC12 type I S-subunit from Lactobacillus delbrueckii (subsp. lactis) (389 aa), FASTA scores: opt: 232, E(): 1.4e-07,(28.1% identity in 185 aa overlap); N-terminus of P72419|sty|SBLI from Salmonella typhimurium (434 aa), FASTA scores: opt: 221, E(): 8e-07, (28.45% identity [...] (364 aa)
dfrADihydrofolate reductase DfrA (DHFR) (tetrahydrofolate dehydrogenase); Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis; Belongs to the dihydrofolate reductase family. (159 aa)
thyAProbable thymidylate synthase ThyA (ts) (TSASE); Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. (263 aa)
Rv2765Rv2765, (MTV002.30), len: 245 aa. Probable ala-rich hydrolase, similar to various hydrolases or hypothetical proteins e.g. Q9KYM6|SC9H11.13c putative hydrolase from Streptomyces coelicolor (251 aa), FASTA scores: opt: 630,E(): 1.4e-33, (43.1% identity in 246 aa overlap); Q9A5T9|CC2358 dienelactone hydrolase family protein from Caulobacter crescentus (286 aa), FASTA scores: opt: 592,E(): 4.5e-31, (38.45% identity in 242 aa overlap); Q9FCF1|2SCD46.33 putative hydrolase (dienelactone hydrolase family) from Streptomyces coelicolor (254 aa), FASTA scores: opt: 500, E(): 3.9e-25, (37.7% iden [...] (245 aa)
drrADaunorubicin-dim-transport ATP-binding protein ABC transporter DrrA; Part of the ABC transporter complex DrrABC involved in doxorubicin resistance. Responsible for energy coupling to the transport system. Binds ATP; Belongs to the ABC transporter superfamily. Drug exporter-1 (DrugE1) (TC 3.A.1.105) family. (331 aa)
drrBDaunorubicin-dim-transport integral membrane protein ABC transporter DrrB; Part of the ABC transporter complex DrrABC involved in doxorubicin resistance. Probably responsible for the translocation of the substrate across the membrane; Belongs to the ABC-2 integral membrane protein family. (289 aa)
Rv3087Possible triacylglycerol synthase (diacylglycerol acyltransferase); Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity. (472 aa)
tgs1Triacylglycerol synthase (diacylglycerol acyltransferase) Tgs1; Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (Probable). (463 aa)
Rv3253cRv3253c, (MTCY20B11.28c), len: 495 aa. Possible cationic amino acid transporter, integral membrane protein,similar to many e.g. O69844|SC1C3.02 putative cationic amino acid transporter from Streptomyces coelicolor (503 aa), FASTA scores: opt: 1649, E(): 5.8e-92, (52.6% identity in 485 aa overlap); Q9AE69 putative transporter (fragment) from Rhodococcus erythropolis (385 aa), FASTA scores: opt: 1594, E(): 9.7e-89, (62.0% identity in 387 aa overlap); Q9PBD7|XF2207 cationic amino acid transporter from Xylella fastidiosa (483 aa), FASTA scores: opt: 1079, E(): 1.2e-57,(40.55% identity in 4 [...] (495 aa)
Rv3254Rv3254, (MTCY20B11.29), len: 462 aa. Conserved hypothetical protein, similar to CAC37877|SC1G7.02 putative secreted protein from Streptomyces coelicolor (440 aa),FASTA scores: opt: 606, E(): 6.2e-31, (31.7% identity in 445 aa overlap); O86550|SC1F2.13c hypothetical 50.7 KDA protein from Streptomyces coelicolor (476 aa), FASTA scores: opt: 577, E(): 4.5e-29, (32.5% identity in 400 aa overlap); Q9L0A8|SCC24.09 putative secreted protein from Streptomyces coelicolor (468 aa), FASTA scores: opt: 380,E(): 1.3e-16, (30.7% identity in 391 aa overlap); BAB48792|MLL1411 probable FAD-dependent mo [...] (462 aa)
glpD2Rv3302c, (MTCI418A.04c, MTV016.01c), len: 585 aa. Probable glpd2, glycerol-3-phosphate dehydrogenase,equivalent to P53435|GLPD_MYCLE|ML0713|L308_C1_179 glycerol-3-phosphate dehydrogenase from Mycobacterium leprae (585 aa), FASTA scores: opt: 3489, E(): 2.2e-198,(90.75% identity in 584 aa overlap). Also highly similar to many e.g. Q9L0I3|SCD63.06 from Streptomyces coelicolor (568 aa), FASTA scores: opt: 2203, E(): 1.6e-122, (59.95% identity in 564 aa overlap); Q9RVK8|DR1019 from Deinococcus radiodurans (522 aa), FASTA scores: opt: 949, E(): 1.4e-48,(37.0% identity in 538 aa overlap); BA [...] (585 aa)
lpdANAD(P)H quinone reductase LpdA; May contribute to virulence by increasing resistance to reactive oxygen intermediates. It can reduce 2,6-dimethyl-1,4- benzoquinone (DMBQ), 5-hydroxy-1,4-naphthaquinone (5-HNQ) and menadione. NADPH is the physiological reductant rather than NADH. (493 aa)
Rv3304Conserved protein; Rv3304, (MTV016.03), len: 159 aa. Conserved protein,very similar to Q9CCL6|ML0711 hypothetical protein from Mycobacterium leprae (159 aa), FASTA scores: opt: 1041,E(): 6.1e-62, (91.8% identity in 159 aa overlap); and Q49927|L308_F3_97 from M. leprae (174 aa), FASTA scores: opt: 974, E(): 1.8e-57, (91.2% identity in 149 aa overlap). Also highly similar to Q9AD81|SCK13.10c conserved hypothetical protein from Streptomyces coelicolor (145 aa),FASTA scores: opt: 615, E(): 7.8e-34, (60.55% identity in 147 aa overlap); and shows some similarity to other various hypothetical [...] (159 aa)
amiA1Rv3305c, (MTV016.04c), len: 389 aa. Possible amiA1,N-acyl-L-amino acid amidohydrolase (or peptidase), similar to many proteins e.g. Q9AK43|2SCK8.09 putative peptidase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 1015, E(): 3.9e-54, (50.8% identity in 374 aa overlap); Q9UZ30|PAB0873 amino acid amidohydrolase from Pyrococcus abyssi (383 aa), FASTA scores: opt: 823, E(): 1.6e-42,(38.2% identity in 369 aa overlap); O58453|PH0722 long hypothetical amino acid amidohydrolase from Pyrococcus horikoshii (388 aa), FASTA scores: opt: 815, E(): 4.8e-42,(38.75% identity in 369 aa overl [...] (389 aa)
amiB1Rv3306c, (MTV016.05c), len: 394 aa. Probable amiB1,aminohydrolase, similar to several belonging to peptidase family M40 (and to hypothetical proteins) e.g. P54983|AMHX_BACSU amidohydrolase AMHX from Bacillus subtilis (389 aa), FASTA scores: opt: 286, E(): 9.9e-10,(26.6% identity in 351 aa overlap); P76052|ABGB_ECOLI Aminobenzoyl-glutamate utilizatio from Escherichia coli (481 aa), FASTA scores: opt: 383, E(): 2.1e-15, (30.5% identity in 328 aa overlap); P44765|YDAJ_HAEIN hypothetical protein HI0584 from Haemophilus influenzae (423 aa), FASTA scores: opt: 297, E(): 2.4e-10, (29.6% ident [...] (394 aa)
cmaA1Cyclopropane mycolic acid synthase 1; Catalyzes the conversion of a double bond to a cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence. (287 aa)
hsaFProbable 4-hydroxy-2-oxovalerate aldolase (HOA); Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta- cleavage pathway for the degradation of aromatic compounds. Belongs to the 4-hydroxy-2-oxovalerate aldolase family. (346 aa)
folP1Dihydropteroate synthase; Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8- dihydropteroate (H2Pte), the immediate precursor of folate derivatives. (280 aa)
Rv3720Possible fatty acid synthase; May be a S-adenosylmethionine-dependent methyltransferase involved in fatty acid metabolism. (420 aa)
Your Current Organism:
Mycobacterium tuberculosis H37Rv
NCBI taxonomy Id: 83332
Other names: M. tuberculosis H37Rv, Mycobacterium sp. H37Rv, Mycobacterium tuberculosis str. H37Rv, Mycobacterium tuberculosis strain H37Rv
Server load: medium (56%) [HD]
STRING is a Core Data Resource as designated by Global Biodata Coalition and ELIXIR.