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| | mtp | Secreted protein antigen; Structural subunit of M.tuberculosis pili (MTP), which are thin (2- to 3-nm wide), flexible, coiled-coil, aggregative fibers. Has a strong affinity for laminin but lacks significant binding affinity for fibronectin or type IV collagen. Mediates adhesion to the extracellular matrix, an event that would facilitate direct interaction with the host epithelium during infection in the lung or other tissues. (103 aa) |
| | groEL1 | 60 kDa chaperonin 1 GroEL1 (protein CPN60-1) (GroEL protein 1); Prevents aggregation of substrate proteins and promotes their refolding. Functions in the absence of co-chaperone CH10 and ATP. (539 aa) |
| | rplM | 50S ribosomal protein L13 RplM; This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. (147 aa) |
| | mrsA | Probable phospho-sugar mutase / MrsA protein homolog; Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate. (448 aa) |
| | mycP1 | Mycosin-1; May play a dual role in regulation of ESX-1 secretion and virulence. Acts as a protease that cleaves EspB. Essential for ESX-1 function, required for early replication in macrophages and full virulence in mice; Belongs to the peptidase S8 family. (446 aa) |
| | esxA | 6 kDa early secretory antigenic target EsxA (ESAT-6); A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL- 12 p40 (IL12B) and TNF-alpha expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protecti [...] (95 aa) |
| | esxB | 10 kDa culture filtrate antigen EsxB (LHP) (CFP10); A secreted protein. Acts as a strong host (human) T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA; native protein binds poorly to artificial liposomes in the absence or presence of EsxA. EsxA and EsxA-EsxB are cytotoxic to pneumocytes. EsxB (and EsxA-EsxB but not EsxA alone) activates human neutrophils; EsxB transiently induces host (human) intracellular Ca(2+) mobility in a dose-depend [...] (100 aa) |
| | crp | Transcriptional regulatory protein Crp (Crp/Fnr-family); Global transcriptional regulator that complexes with cAMP and binds to specific DNA promoter sites, causing DNA-bending, to regulate transcription. cAMP improves binding to specific DNA sequences, probably by altering protein conformation. The CRP regulon is predicted to contain about 115 genes. Some genes are activated by CRP (rpfA, whiB1) while others are repressed (fadD10). There are 2 CRP-binding sites in the promoter of whiB1, at low concentrations of CRP with or without cAMP transcription of whiB1 is enhanced via site CRP1, [...] (224 aa) |
| | ftsH | Membrane-bound protease FtsH (cell division protein); Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins. (760 aa) |
| | lsr2 | Iron-regulated H-NS-like protein Lsr2; DNA-bridging protein that has both architectural and regulatory roles. Influences the organization of chromatin and gene expression by binding non-specifically to DNA, with a preference for AT-rich sequences, and bridging distant DNA segments. Binds in the minor groove of AT-rich DNA. Represses expression of multiple genes involved in a broad range of cellular processes, including major virulence factors or antibiotic-induced genes, such as iniBAC or efpA , and genes important for adaptation of changing O(2) levels. May also activate expression of [...] (112 aa) |
| | ltp3 | Rv3523, (MTCY03C7.33c), len: 394 aa. Probable ltp3,lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30037|AF0202 3-ketoacyl-CoA thiolase (ACAB-6) from Archaeoglobus fulgidus (380 aa) FASTA scores: opt: 782, E(): 1.7e-40, (38.35% identity in 386 aa overlap); Q9Y9A1|APE2384 long hypothetical non specific lipid-transfer protein (acethyl CoA synthetase) from Aeropyrum pernix (394 aa), FASTA scores: opt: 626, E(): 5.9e-31, (35.75% identity in 386 aa overlap); BAB59210|TVG0067506 lipid transfer protein from Thermoplasma volcanium (390 aa), FASTA scores: opt: 591,E(): [...] (394 aa) |
| | pknB | Transmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B); Protein kinase that regulates many aspects of mycobacterial physiology, and is critical for growth in vitro and survival of the pathogen in the host. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins such as GarA, GlmU, PapA5, PbpA, FhaB (Rv0019c), FhaA (Rv0020c), MviN, PstP, EmbR, Rv1422, Rv1747 and RseA. Also catalyzes the phosphorylation of the core proteasome alpha-subunit (PrcA), and thereby regulates th [...] (626 aa) |
| | fhaA | Conserved protein with FHA domain, FhaA; Regulates cell growth and peptidoglycan synthesis by binding to MviN. May inhibit the late stages of peptidoglycan synthesis. (527 aa) |
| | Rv0021c | Rv0021c, (MTCY10H4.21c), len: 322 aa. Conserved hypothetical protein, similar to various proteins. (322 aa) |
| | Rv0038 | Conserved protein; Rv0038, (MTCY10H4.38), len: 202 aa. Conserved protein, similar to many; Belongs to the UPF0301 (AlgH) family. (202 aa) |
| | Rv0081 | Rv0081, (MTV030.25), len: 114 aa. Probable transcriptional regulator, highly similar to others. (114 aa) |
| | Rv0097 | Rv0097, (MTCY251.16), len: 289 aa. Possible oxidoreductase, equivalent to NP_302343.1|NC_002677 putative oxidoreductase from Mycobacterium leprae (289 aa). Also highly similar to BAB69377.1|AB070955 putative oxidoreductase from Streptomyces avermitilis (296 aa). Contains PS00077 Cytochrome c oxidase subunit I, copper B binding region signature. (289 aa) |
| | Rv0195 | Rv0195, (MTV033.03), len: 211 aa. Possible two-component response regulator, luxR family, similar to many e.g. U00008|ECOHU49_15 regulatory protein narP from Escherichia coli strain K12 (225 aa), FASTA scores: opt: 232, E(): 7.3e-09, (29.2% identity in 219 aa overlap). Start chosen by similarity. Contains probable helix-turn-helix motif at aa 166-187 (Score 1164, +3.15 SD). (211 aa) |
| | Rv0199 | Rv0199, (MTV033.07), len: 219 aa. Probable conserved membrane protein, equivalent to Z95398|MLCL622.13 from Mycobacterium leprae (224 aa), FASTA scores: opt: 920, E(): 0, (67.7% identity in 220 aa overlap). Also some similarity to Mce-associated membrane proteins from Mycobacterium tuberculosis e.g. Rv0178, Rv0175, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (219 aa) |
| | mmpL11 | Probable conserved transmembrane transport protein MmpL11; Part of a heme-iron acquisition system. Receives heme from the heme-binding protein Rv0203 and transports it into the mycobacterial cell. Contributes to virulence. Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily. (966 aa) |
| | fabG4 | Rv0242c, (MTV034.08c), len: 454 aa. Probable fabG4,3-oxoacyl-[acyl-carrier protein] reductase, equivalent to 3063883|CAA18568.1|AL022486|MLCB1883_13|T44878 3-oxoacyl-[acyl-carrier protein] reductase homolog from Mycobacterium leprae (454 aa), FASTA scores: opt: 2486,E(): 0, (84.8% identity in 454 aa overlap). C-terminal part highly similar to many FabG proteins e.g. U39441|VHU3944 1_2 from Vibrio harveyi (244 aa), FASTA scores: opt: 562,E(): 3.4e-28, (40.2% identity in 241 aa overlap); U91631|PAU91631_3 from Pseudomonas aeruginosa (247 aa),FASTA scores: opt: 584, E(): 1.5e-29, (44.4% i [...] (454 aa) |
| | nirB | Rv0252, (MTV034.18), len: 853 aa. Probable nirB (alternate gene name: nasB), nitrite reductase [NAD(P)H] large subunit, flavoprotein containing siroheme and a 2FE-2S iron-sulfur centre. Highly similar to many others bacterial enzymes e.g. P08201|NIRB_ECOLI nitrite reductase (NAD(P)H) large subunit from Escherichia coli strain K12 (847 aa), FASTA scores: opt: 2775, E(): 0, (49.8% identity in 840 aa overlap); Q06458|NIRB_KLEPN nitrite reductase (NAD(P)H) large subunit (957 aa), FASTA scores: opt: 2902,E(): 0, (54.2% identity in 827 aa overlap). Contains PS00365 Nitrite and sulfite reduct [...] (853 aa) |
| | pknG | Serine/threonine-protein kinase PknG (protein kinase G) (STPK G); Phosphorylates GarA. May play a role in metabolic regulation via control of the phosphorylation status of GarA. Plays a crucial role in the survival of mycobacteria within host macrophages, by blocking the intracellular degradation of mycobacteria in lysosomes. Required for intrinsic antibiotic resistance. (750 aa) |
| | mutT3 | Putative 8-oxo-dGTP diphosphatase 3; May be involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8-oxo- dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT specifically degrades 8-oxo- dGTP to the monophosphate (By similarity). (217 aa) |
| | icl1 | Isocitrate lyase Icl (isocitrase) (isocitratase); Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It could also catalyze the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route) (By similarity). (428 aa) |
| | hbhA | Iron-regulated heparin binding hemagglutinin HbhA (adhesin); Required for extrapulmonary dissemination. Mediates adherence to epithelial cells by binding to sulfated glycoconjugates present at the surface of these cells; binds heparin, dextran sulfate, fucoidan and chondroitin sulfate. Promotes hemagglutination of erythrocytes of certain host species. Induces mycobacterial aggregation. To M.leprae HbhA. (199 aa) |
| | regX3 | Sensory transduction protein RegX3; Probably forms part of a two-component regulatory system RegX3/SenX3. (227 aa) |
| | ccdA | Rv0527, (MTCY25D10.06), len: 259 aa. Possible ccdA,cytochrome C-type biogenesis protein, integral membrane protein, equivalent to Q49810|B2168_C1_192|S72890 hypothetical protein from Mycobacterium leprae (262 aa),FASTA scores: opt: 1341, E(): 0, (79.0% identity in 262 aa overlap). Also highly similar to others e.g. CAC08380.1 (253 aa); CCDA_BACSU|P45706 cytochrome C-type biogenesis protein from Bacillus subtilis (235 aa), FASTA scores: opt: 307, E(): 7.4e-13, (30.4% identity in 237 aa overlap); etc. Seems to belong to the DSBD subfamily. Note that previously known as ccsA. (259 aa) |
| | Rv0566c | Conserved protein; Rv0566c, (MTV039.04c), len: 163 aa. Conserved protein, similar to others e.g. P77482|YAJQ_ECOLI hypothetical 19.0 KDa protein from Escherichia coli (169 aa), FASTA scores: opt: 422, E(): 5.4e-20, (44.1 identity in 161 aa overlap); etc; Belongs to the UPF0234 family. (163 aa) |
| | Rv0574c | Conserved hypothetical protein; Could be involved in the biosynthesis, transport or localization of poly-alpha-L-glutamine (PLG), a cell wall component. Contributes to stress tolerance and virulence. (380 aa) |
| | narL | Possible nitrate/nitrite response transcriptional regulatory protein NarL; Member of the two-component regulatory system NarS/NarL that regulates genes involved in aerobic nitrate metabolism. Upon phosphorylation by NarS, functions as a transcription regulator by direct binding to promoter regions of target genes together with DevR to regulate their expression during aerobic nitrate metabolism. (216 aa) |
| | mprA | Response regulator MprA; Member of the two-component regulatory system MprB/MprA which contributes to maintaining a balance among several systems involved in stress resistance and is required for establishment and maintenance of persistent infection in the host. Functions as a transcriptional regulator that recognizes a 19-bp nucleotide motif comprizing two loosely conserved 8-bp direct DNA-binding motif repeats separated by a 3-bp spacer region. MprB/MprA is involved in regulation of numerous stress-responsive genes, including up-regulation of two sigma factors, sigE and sigB as well [...] (228 aa) |
| | mprB | Two component sensor kinase MprB; Member of the two-component regulatory system MprB/MprA which contributes to maintaining a balance among several systems involved in stress resistance and is required for establishment and maintenance of persistent infection in the host. In response to environmental signals MprB acts as both a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to MprA, and a protein phosphatase that dephosphorylates phospho-MprA. MprB/MprA is involved in regulation of numerous stress-responsive genes, includin [...] (504 aa) |
| | galU | Rv0993, (MTCI237.07), len: 306 aa. GalU,UTP--glucose-1-phosphate uridylyltransferase, equivalent to AL035500|MLCL373_22 putative UTP-glucose-1-phosphate uridylyltransferase from Mycobacterium leprae (306 aa),FASTA score: (89.7% identity in 302 aa overlap). Also highly similar to others e.g. AB59678.1|AL132674 UTP-glucose-1-phosphate uridylyltransferase from Streptomyces coelicolor (303 aa); NP_244519.1|NC_002570 UTP-glucose-1-phosphate uridylyltransferase from Bacillus halodurans (297 aa); P25520|GALU_ECOLI|B1236|Z2012|ECS17 UTP--glucose-1-phosphate uridylyltransferase from Escherichia [...] (306 aa) |
| | pks16 | Rv1013, (MTCI237.30-MTCY10G2.36c), len: 544 aa. Putative pks16, polyketide synthase, similar to many e.g. N-terminus of Q50857|U24657 saframycin MX1 synthetase B (1770 aa), FASTA scores: opt: 526, E(): 1.4e-25, (29.3% identity in 542 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. (544 aa) |
| | glmU | Probable UDP-N-acetylglucosamine pyrophosphorylase GlmU; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain. (495 aa) |
| | Rv1049 | Rv1049, (MTV017.02), len: 148 aa. Probable transcriptional repressor protein, similar to many e.g. P74870 negative regulator of EMR locus EMR from Salmonella typhimurium (149 aa), FASTA scores: opt: 146, E(): 0.0011,(31.6% identity in 95 aa overlap). Contains probable helix-turn-helix motif at aa 58-79 (Score 1495, +4.28 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). (148 aa) |
| | mazF3 | Toxin MazF3; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an mRNA and 23S rRNA interferase, cleaving predominantly after the first 2 Us in the sequence 5'-UUCCU-3'; in 23S rRNA only cleaves once in the ribosomal A site in dissociated but not intact ribosomes. Cleavage of 23S rRNA inhibits protein translation; the 23S rRNA region cleaved is involved in tRNA-binding in the A site, 30S and 50S subunit interaction and ribosome recycling factor association. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. It dramatically increases per [...] (103 aa) |
| | cobB-2 | Transcriptional regulatory protein; NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form (By similarity). Reactivates acetylated acetyl-CoA synthetase (ACS) through an NAD-dependent deacetylation. Is able to ADP-ribosylate itself. (237 aa) |
| | typA | GTP-binding protein TypA/BipA; Rv1165, (MTV005.01-MTCI65.32), len: 628 aa. Possible typA (alternate gene name: bipA), GTP-binding translation elongation factor, similar to several e.g. P32132|TYPA_ECOLI|BIPA|B387 Escherichia coli (591 aa); YIHK_SYNY3|P72749 gtp-binding protein TYPA/BIPA homolog from synechocystis sp. (597 aa), FASTA scores: E(): 0,(46.9% identity in 610 aa overlap); and to elongation factor EF-G from many organims e.g. EFG_MICLU|P09952 micrococcus luteus (701 aa), FASTA scores: E(): 3e-24,(29.8% identity in 500 aa overlap). Belongs to the GTP-binding elongation factor [...] (628 aa) |
| | Rv1176c | Rv1176c, (MTV005.12c), len: 189 aa. Conserved hypothetical protein, some similarity to P94443|D78508 hypothetical protein from Bacillus subtilis (182 aa), FASTA scores: opt: 219, E(): 1.7e-15, (25.1% identity in 183 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein Rv0047c. (189 aa) |
| | lpqY | Probable sugar-binding lipoprotein LpqY; Part of the ABC transporter complex LpqY-SugA-SugB-SugC, which is highly specific for uptake of trehalose. Involved in the recycling of extracellular trehalose released from trehalose-containing molecules synthesized by M.tuberculosis. Trehalose uptake is essential for virulence; Belongs to the bacterial solute-binding protein 1 family. (468 aa) |
| | sugA | Probable sugar-transport integral membrane protein ABC transporter SugA; Part of the ABC transporter complex LpqY-SugA-SugB-SugC, which is highly specific for uptake of trehalose. Involved in the recycling of extracellular trehalose released from trehalose-containing molecules synthesized by M.tuberculosis. Trehalose uptake is essential for virulence. Probably responsible for the translocation of the substrate across the membrane. (307 aa) |
| | sugB | Probable sugar-transport integral membrane protein ABC transporter SugB; Part of the ABC transporter complex LpqY-SugA-SugB-SugC, which is highly specific for uptake of trehalose. Involved in the recycling of extracellular trehalose released from trehalose-containing molecules synthesized by M.tuberculosis. Trehalose uptake is essential for virulence. Probably responsible for the translocation of the substrate across the membrane; Belongs to the binding-protein-dependent transport system permease family. (274 aa) |
| | sugC | Probable sugar-transport ATP-binding protein ABC transporter SugC; Part of the ABC transporter complex LpqY-SugA-SugB-SugC, which is highly specific for uptake of trehalose. Involved in the recycling of extracellular trehalose released from trehalose-containing molecules synthesized by M.tuberculosis. Trehalose uptake is essential for virulence. Probably responsible for energy coupling to the transport system. (393 aa) |
| | Rv1354c | Conserved hypothetical protein; Rv1354c, (MTCY02B10.18c), len: 623 aa. Conserved hypothetical protein, similar to many hypothetical proteins e.g. the C-terminus of G1001455 Synechocystis sp. (1244 aa), FASTA scores: opt: 933, E(): 0, (36.8% identity in 462 aa overlap); also similar to Rv1357c|MTCY02B10.21c (34.0% identity in 253 aa overlap). (623 aa) |
| | Rv1357c | Conserved hypothetical protein; Rv1357c, (MTCY02B10.21c), len: 307 aa. Conserved hypothetical protein, similar to members of the YEGE/YHJK/YJCC family e.g. Y4LL_RHISN|P55552 hypothetical protein Y4ll from Rhizobium sp. (827 aa), FASTA scores: E(): 0, (37.7% identity in 257 aa overlap), also similar to Rv1354c|MTCY02B10.18c (34.0% identity in 253 aa overlap). Belongs to the YEGE/YHDA/YHJK/YJCC family. (307 aa) |
| | Rv1358 | Rv1358, (MTCY02B10.22), len: 1159 aa. Probable transcriptional regulatory protein, some similarity to AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 210, E(): 5.5e-06,(27.5% identity in 739 aa overlap). Similar also to Rv0890C|MTCY31.18c (65.5% identity in 884 aa overlap) and to Rv1359|MTCY02B10.23 (43.7% identity in 197 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00622 Bacterial regulatory proteins, luxR family signature. Helix turn helix motif present at aa 1116-1137, (Score 1291,+3.59 SD). (1159 aa) |
| | pknF | Anchored-membrane serine/threonine-protein kinase PknF (protein kinase F) (STPK F); Phosphorylates the FHA domains of the ABC transporter Rv1747, the heat-shock protein GroEL 1, and Rv0020c. May play a role in the regulation of glucose transport, cell growth and septum formation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. (476 aa) |
| | secA2 | Possible preprotein translocase ATPase SecA2; Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane. (808 aa) |
| | mazF6 | Toxin MazF6; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis partially inhibits cell growth and colony formation; its toxic effect is neutralized by coexpression with cognate antitoxin MazE6. Acts as an mRNA interferase on ssRNA, cleaving between the second and third bases in the sequences CUCCU and UUCCU. Further experiments demonstrate that it digests between the first and second bases of UCCUU, yielding a 5'- hydroxyl end; digests M.tuberculosis mRNA (in coding as well as the 5'- and 3'-UTR regions) and 23S rRNA, digests E.coli [...] (114 aa) |
| | Rv1996 | Rv1996, (MTCY39.23c), len: 317 aa. Universal stress protein family protein. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv2005c|Q10851|YK05_MYCTU (295 aa), FASTA scores: opt: 775,E(): 0, (50.3% identity in 316 aa overlap); Rv2026c (294 aa) (47.9% identity in 311 aa overlap); and Rv2623, etc. Also similar to SCJ1.30c|AL109962 hypothetical protein from Streptomyces coelicolor (328 aa). Predicted possible vaccine candidate (See Zvi et al., 2008). (317 aa) |
| | helY | Rv2092c, (MTCY49.32c), len: 906 aa. HelY,ATP-dependent DNA helicase, similar to many; contains PS00017 ATP/GTP-binding site motif A, PS00402 Binding-protein-dependent transport systems inner membrane component signature. Belongs to the SKI2 subfamily of helicases. (906 aa) |
| | uppP | Possible conserved transmembrane protein; Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin. (276 aa) |
| | Rv2216 | Conserved protein; Rv2216, (MTCY190.27), len: 301 aa. Conserved protein, equivalent to Mycobacterium leprae ML0860 (307 aa), Z98741|MLCB22_10 Mycobacterium leprae cosmid B22; H (307 aa). FASTA score: opt: 1656, E(): 0; 84.2% identity in 297 aa overlap. Also gp|AE000319|ECAE000319_8 Escherichia coli strain K12 MG1655 (297 aa) opt: 640, E(): 0; 39.5% identity in 294 aa overlap; Belongs to the NAD(P)-dependent epimerase/dehydratase family. SDR39U1 subfamily. (301 aa) |
| | kasA | 3-oxoacyl-[acyl-carrier protein] synthase 1 KasA (beta-ketoacyl-ACP synthase) (KAS I); Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family. (416 aa) |
| | kasB | 3-oxoacyl-[acyl-carrier protein] synthase 2 KasB (beta-ketoacyl-ACP synthase) (KAS I); Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. (438 aa) |
| | dhmA1 | Probable haloalkane dehalogenase; Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons; Belongs to the haloalkane dehalogenase family. Type 1 subfamily. (300 aa) |
| | moeW | Rv2338c, (MTCY98.07c), len: 318 aa. Possible moeW,molybdoptenum biosynthesis protein, showing some similarity to several molybdopterin biosynthesis proteins e.g. O27613|MTH1571 molybdopterin biosynthesis protein MOEB homolog from Methanobacterium thermoautotrophicum (251 aa),FASTA scores: opt: 309, E(): 4.7e-14; (30.7% identity in 254 aa overlap); Q9KPQ5|VC2311 HESA/MOEB/THIF family protein from Vibrio cholerae (273 aa), FASTA scores: opt: 255, E(): 4e-09, (36.25% identity in 149 aa overlap); Q9PD34|XF1545 molybdopterin biosynthesis protein from Xylella fastidiosa (276 aa), FASTA score [...] (318 aa) |
| | aroK | Shikimate kinase AroK (SK); Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. Belongs to the shikimate kinase family. (176 aa) |
| | ppiB | Probable peptidyl-prolyl cis-trans isomerase B; PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity). (308 aa) |
| | relA | Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase; In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the formation of pppGpp, which is then hydrolyzed to form ppGpp, as well as the hydrolysis of ppGpp. RelA is probably a key factor in the pathogenesis of M.tuberculosis as it regulates the intracellular concentrations of (p)ppGpp. (790 aa) |
| | sigB | RNA polymerase sigma factor SigB; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. A non-essential principal sigma factor that responds to cell envelope stress and hypoxia. Controls a regulon of about 40 genes, with another 100 genes expression being altered during SDS stress and about 50 gene being altered during diamide (oxidative) stress. (323 aa) |
| | pknI | Serine/threonine-protein kinase PknI; Plays an important role in slowing down the growth of mycobacteria within the infected host; Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. (585 aa) |
| | pks1 | Probable polyketide synthase Pks1; May play a role in phthiocerol biosynthesis. (1616 aa) |
| | Rv2959c | Possible methyltransferase (methylase); Catalyzes the O-methylation of the hydroxyl group located on C-2 of the first rhamnosyl residue linked to the phenolic group of glycosylated phenolphthiocerol dimycocerosates (PGL) and p- hydroxybenzoic acid derivatives (p-HBAD). (245 aa) |
| | nrdH | Probable glutaredoxin electron transport component of NRDEF (glutaredoxin-like protein) NrdH; Rv3053c, (MTCY22D7.29), len: 79 aa. Probable nrdH,glutaredoxin-like protein, equivalent to Q9CBP8|NRDH|ML1736 from Mycobacterium leprae (80 aa), FASTA scores: opt: 478,E(): 2.7e-27, (91.15% identity in 79 aa overlap), and similar to many glutaredoxin-like proteins e.g. Q9XD65|NRDH from Corynebacterium glutamicum (Brevibacterium flavum) (77 aa), FASTA scores: opt: 382, E(): 1.5e-20, (72.35% identity in 76 aa overlap); and Q56108|NRDH_SALTY from Salmonella typhimurium (81 aa), FASTA scores: opt: [...] (79 aa) |
| | devR | Two component transcriptional regulatory protein DevR (probably LuxR/UhpA-family); Member of the two-component regulatory system DevR/DevS (also called DosR/DosS) involved in onset of the dormancy response. Regulates an approximately 48-member regulon. When phosphorylated binds and activates the promoter of DevR regulon genes in response to hypoxia. The presence of target DNA increases stability of phospho-DevR in vitro. Activates its own transcription under hypoxic but not aerobic conditions, probably binds as a dimer to tandem binding sites within the devR and hspX promoters. Accepts [...] (217 aa) |
| | Rv3295 | Probable transcriptional regulatory protein (probably TetR-family); Rv3295, (MTCY71.35), len: 221 aa. Probable transcriptional regulator TetR-family, equivalent to Q9CCL4|ML0717 putative TetR-family transcriptional regulator from Mycobacterium leprae (223 aa), FASTA scores: opt: 1260, E(): 7.2e-75, (85.45% identity in 220 aa overlap). Also highly similar to other streptomyces regulators e.g. Q9RD77|SCF43.11 from Streptomyces coelicolor (205 aa), FASTA scores: opt: 442, E(): 9.8e-22,(38.6% identity in 202 aa overlap); Q9RKY8|SC6D7.09 from Streptomyces coelicolor (220 aa), FASTA scores: [...] (221 aa) |
