STRINGSTRING
wag31 wag31 ftsZ ftsZ ftsQ ftsQ mmpS3 mmpS3 smtB smtB ideR ideR ftsK ftsK ftsY ftsY hupB hupB prfB prfB ftsE ftsE mtrA mtrA infA infA lsr2 lsr2 espR espR espF espF esxB esxB espL espL espB espB mviN mviN cwlM cwlM dnaA dnaA dnaN dnaN pknB pknB pknA pknA fhaA fhaA ssb ssb mmpL3 mmpL3 grpE grpE secE1 secE1 tuf tuf phoP phoP greA greA rho rho mihF mihF infC infC fbpB fbpB tatA tatA
Nodes:
Network nodes represent proteins
splice isoforms or post-translational modifications are collapsed, i.e. each node represents all the proteins produced by a single, protein-coding gene locus.
Node Color
colored nodes:
query proteins and first shell of interactors
white nodes:
second shell of interactors
Node Content
empty nodes:
proteins of unknown 3D structure
filled nodes:
a 3D structure is known or predicted
Edges:
Edges represent protein-protein associations
associations are meant to be specific and meaningful, i.e. proteins jointly contribute to a shared function; this does not necessarily mean they are physically binding to each other.
Known Interactions
from curated databases
experimentally determined
Predicted Interactions
gene neighborhood
gene fusions
gene co-occurrence
Others
textmining
co-expression
protein homology
Your Input:
wag31Diviva family protein Wag31; Important for maintaining cell shape and cell wall integrity by localizing peptidoglycan synthesis to the cell poles. Protects PbpB (PBP3, FtsI) from oxidative stress-induced cleavage. (260 aa)
ftsZCell division protein FtsZ; Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity. (379 aa)
ftsQPossible cell division protein FtsQ; Essential cell division protein. (314 aa)
mmpS3Rv2198c, (MTCY190.09c), len: 299 aa. Probable mmpS3,conserved membrane protein (see citation below), equivalent to ML0877|mmpS3 putative membrane protein from Mycobacterium leprae (293 aa), FASTA scores: opt: 1089,E(): 1.2e-43, (69.80% identity in 308 aa overlap). Also similar to other proteins e.g. Rv3209 from Mycobacterium tuberculosis. Contains PS00499 C2 domain signature, a hydrophobic region, and a repetitive proline and threonine rich region. Belongs to the MmpS family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (299 aa)
smtBProbable transcriptional regulatory protein SmtB (probably ArsR-family); Transcriptional regulator involved in zinc homeostasis. Represses the expression of the smtB-zur operon in the absence of zinc. Could act as the metal sensor that controls the expression of zur in response to zinc availability. (135 aa)
ideRIron-dependent repressor and activator IdeR; Metal-dependent DNA-binding protein that controls transcription of many genes involved in iron metabolism. Acts as a repressor of siderophore biosynthesis and as a positive modulator of iron storage. Also regulates expression of transporters, proteins involved in siderophore synthesis, iron storage and transcriptional regulators. (230 aa)
ftsKPossible cell division transmembrane protein FtsK; Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Required for activation of the Xer recombinase, allowing activation of chromosome unlinking by recombination (By similarity). (883 aa)
ftsYProbable cell division protein FtsY (SRP receptor) (signal recognition particle receptor); Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). (422 aa)
hupBDNA-binding protein HU homolog HupB (histone-like protein) (HLP) (21-kDa laminin-2-binding protein); Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. Binds DNA non-specifically. Induces lymphoproliferation, particularly in health tuberculin reactors, and is immunogenic. Maybe involved in pathogenesis of inflammatory bowel disease (IBD) in patients with ulcerative colitis and Crohn disease (CD). Bound by anti-neutrophil cytoplasmic antibodies (pANCA), which are a hallmark of IB [...] (214 aa)
prfBProbable peptide chain release factor 2 PrfB (RF-2); Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. (378 aa)
ftsECell division ATP-binding protein FtsE; Part of the ABC transporter FtsEX involved in cellular division. Has ATPase activity. (229 aa)
mtrATwo component sensory transduction transcriptional regulatory protein MtrA; Member of the two-component regulatory system MtrA/MtrB. Binds direct repeat motifs of sequence 5'-GTCACAGCG-3', phosphorylation confers higher affinity. Overexpression decreases bacteria viability upon infection of human THP-1 macrophage cell line, due at least in part to impaired blockage of phagosome-lysosome fusion (upon infection bacteria usually remain in phagosomes). Infecting C57BL/6 mice with an overexpressing strain leads to an attentuated infection in both spleen and lungs. The level of dnaA mRNA inc [...] (228 aa)
infAProbable translation initiation factor if-1 InfA; One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. (73 aa)
lsr2Iron-regulated H-NS-like protein Lsr2; DNA-bridging protein that has both architectural and regulatory roles. Influences the organization of chromatin and gene expression by binding non-specifically to DNA, with a preference for AT-rich sequences, and bridging distant DNA segments. Binds in the minor groove of AT-rich DNA. Represses expression of multiple genes involved in a broad range of cellular processes, including major virulence factors or antibiotic-induced genes, such as iniBAC or efpA , and genes important for adaptation of changing O(2) levels. May also activate expression of [...] (112 aa)
espRESX-1 transcriptional regulatory protein EspR; Virulence regulator that has both architectural and regulatory roles. Impacts cell wall functions and pathogenesis through regulation of multiple genes, including the espACD operon, which is a key ESX-1 component. Influences target gene expression positively or negatively, depending on its binding position relative to the genes it controls. Acts by binding directly to the DNA. May play a central role in regulating virulence gene expression. (132 aa)
espFRv3865, (MTCY01A6.03c), len: 103 aa. EspF, ESX-1 secretion-associated protein, showing some similarity to O06268|Rv3615c|MTCY07H7B.07 hypothetical 10.8 KDA protein from Mycobacterium tuberculosis (103 aa), FASTA scores: opt: 198, E(): 7.5e-07, (36.25% identity in 102 aa overlap); Q49723|ML0406|B1620_C2_214|MLCL383.02 hypothetical 11.1 KDA protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 154, E(): 0.00071, (31.05% identity in 103 aa overlap); Belongs to the EspC family. (103 aa)
esxB10 kDa culture filtrate antigen EsxB (LHP) (CFP10); A secreted protein. Acts as a strong host (human) T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA; native protein binds poorly to artificial liposomes in the absence or presence of EsxA. EsxA and EsxA-EsxB are cytotoxic to pneumocytes. EsxB (and EsxA-EsxB but not EsxA alone) activates human neutrophils; EsxB transiently induces host (human) intracellular Ca(2+) mobility in a dose-depend [...] (100 aa)
espLESX-1 secretion-associated protein EspL; Probably plays a role in host phagosome maturation arrest. (115 aa)
espBSecreted ESX-1 substrate protein B, EspB. Conserved alanine and glycine rich protein; Required for host-cell death and may support an EsxA- independent virulence function. Secreted processed form of EspB binds to phosphatidic acid and phosphatidylserine. Inhibits IFN-gamma-induced autophagy in murine macrophages. (460 aa)
mviNProbable conserved transmembrane protein; Essential for cell growth and peptidoglycan synthesis. In the N-terminal section; belongs to the MurJ/MviN family. (1184 aa)
cwlMProbable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide. (406 aa)
dnaAChromosomal replication initiator protein DnaA; Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'- TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity). Binds its own promoter. (507 aa)
dnaNDNA polymerase III (beta chain) DnaN (DNA nucleotidyltransferase); Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP- independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta ch [...] (402 aa)
pknBTransmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B); Protein kinase that regulates many aspects of mycobacterial physiology, and is critical for growth in vitro and survival of the pathogen in the host. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins such as GarA, GlmU, PapA5, PbpA, FhaB (Rv0019c), FhaA (Rv0020c), MviN, PstP, EmbR, Rv1422, Rv1747 and RseA. Also catalyzes the phosphorylation of the core proteasome alpha-subunit (PrcA), and thereby regulates th [...] (626 aa)
pknATransmembrane serine/threonine-protein kinase A PknA (protein kinase A) (STPK A); Protein kinase that regulates many aspects of mycobacterial physiology, and is critical for growth in vitro and survival of the pathogen in the host. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins such as FtsZ, Wag31, GlmU, FhaB, PstP, EmbR and Rv1422. Also catalyzes the phosphorylation of the proteasome alpha-subunit (PrcA) and unprocessed proteasome beta-subunit (pre-PrcB), which results in the inhibitio [...] (431 aa)
fhaAConserved protein with FHA domain, FhaA; Regulates cell growth and peptidoglycan synthesis by binding to MviN. May inhibit the late stages of peptidoglycan synthesis. (527 aa)
ssbRv0054, (MTCY21D4.17), len: 164 aa. ssb,single-strand binding protein (see Mizrahi & Andersen 1998), highly similar to others. Belongs to the SSB family. (164 aa)
mmpL3Possible conserved transmembrane transport protein MmpL3; Transports trehalose monomycolate (TMM) across the inner membrane. Could also be part of a heme-iron acquisition system. Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily. (944 aa)
grpEProbable GrpE protein (HSP-70 cofactor); Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Severa [...] (235 aa)
secE1Probable preprotein translocase SecE1; Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation. (161 aa)
tufProbable iron-regulated elongation factor TU Tuf (EF-TU); This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (396 aa)
phoPRv0757, (MTCY369.02), len: 247 aa. Possible phoP,two component system response phosphate regulon transcriptional regulator (see citations below), highly similar to various transcriptional regulators e.g. CAC32360.1|AL583945 putative two component system response regulator from Streptomyces coelicolor (271 aa); T45446 probable two-component response regulator from Mycobacterium leprae (253 aa); and similar to phoP proteins e.g. P13792|PHOP_BACSU alkaline phosphatase synthesis transcription regulatory protein from Bacillus subtilis (240 aa), FASTA scores: opt: 594, E(): 2.3e-33, (41.0% i [...] (247 aa)
greAProbable transcription elongation factor GreA (transcript cleavage factor GreA); Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. (164 aa)
rhoProbable transcription termination factor Rho homolog; Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA- dependent ATPase activity, and release of the mRNA from the DNA template. Shows poor RNA-dependent ATP hydrolysis and inefficient DNA- RNA unwinding activities, but exhibits robust and fast transcription termination, which suggests that the transcription termination function of M.tuberculosis Rho is not correlated with its helicase/translocase activities and that these functions may not be important for its RN [...] (602 aa)
mihFRv1388, (MTCY21B4.05), len: 190 aa. Putative mihF,integration host factor. Almost identical to, but longer than, P96802|U75344 Mycobacterium smegmatis integration host factor (mIHF) for mycobacteriophage L5 (105 aa), FASTA scores: E(): 0, (96.1% identity in 102 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). (190 aa)
infCProbable initiation factor if-3 InfC; IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. (201 aa)
fbpBDiacylglycerol acyltransferase/mycolyltransferase Ag85B; The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha- trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-treha [...] (325 aa)
tatASec-independent protein translocase membrane-bound protein TatA; Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin- arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system. Belongs to the TatA/E family. (83 aa)
Your Current Organism:
Mycobacterium tuberculosis H37Rv
NCBI taxonomy Id: 83332
Other names: M. tuberculosis H37Rv, Mycobacterium sp. H37Rv, Mycobacterium tuberculosis str. H37Rv, Mycobacterium tuberculosis strain H37Rv
Server load: low (12%) [HD]
STRING is a Core Data Resource as designated by Global Biodata Coalition and ELIXIR.