Rv1049, (MTV017.02), len: 148 aa. Probable transcriptional repressor protein, similar to many e.g. P74870 negative regulator of EMR locus EMR from Salmonella typhimurium (149 aa), FASTA scores: opt: 146, E(): 0.0011,(31.6% identity in 95 aa overlap). Contains probable helix-turn-helix motif at aa 58-79 (Score 1495, +4.28 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).

Iron-dependent repressor and activator IdeR; Metal-dependent DNA-binding protein that controls transcription of many genes involved in iron metabolism. Acts as a repressor of siderophore biosynthesis and as a positive modulator of iron storage. Also regulates expression of transporters, proteins involved in siderophore synthesis, iron storage and transcriptional regulators.

Rv3916c, (MTV028.07c), len: 244 aa. Conserved hypothetical protein, equivalent to Q50200|ML2705|L222-ORF1 hypothetical protein from Mycobacterium leprae (259 aa),FASTA scores: opt: 1266, E(): 2e-74, (76.4% identity in 250 aa overlap). Also highly similar (but with gaps) to Q9R3S2|STH24.10 hypothetical 22.6 KDA protein from Streptomyces coelicolor (205 aa), FASTA scores: opt: 387,E(): 7.5e-18, (40.25% identity in 231 aa overlap). Predicted to be an outer membrane protein (See Song et al.,2008).

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Rv3916c, (MTV028.07c), len: 244 aa. Conserved hypothetical protein, equivalent to Q50200|ML2705|L222-ORF1 hypothetical protein from Mycobacterium leprae (259 aa),FASTA scores: opt: 1266, E(): 2e-74, (76.4% identity in 250 aa overlap). Also highly similar (but with gaps) to Q9R3S2|STH24.10 hypothetical 22.6 KDA protein from Streptomyces coelicolor (205 aa), FASTA scores: opt: 387,E(): 7.5e-18, (40.25% identity in 231 aa overlap). Predicted to be an outer membrane protein (See Song et al.,2008).

Rv3913, (MT4032, MTV028.04), len: 335 aa. Probable trxB2, thioredoxin reductase (see citation below),equivalent to O30973|TRXB_MYCSM thioredoxin reductase from Mycobacterium smegmatis (311 aa), FASTA scores: opt: 1575,E(): 1.8e-87, (78.35% identity in 305 aa overlap); and highly similar, but shorter at C-terminus, to P46843|TRXB_MYCLE|TRXB/a|TRX|ML2703 bifunctional thioredoxin reductase/thioredoxin from Mycobacterium leprae (458 aa), FASTA scores: opt: 1766, E(): 8.7e-99, (83.25% identity in 328 aa overlap). Also highly similar to many e.g. P52215|TRXB_STRCO|SCH24.12 from Streptomyces [...]

Rv3916c, (MTV028.07c), len: 244 aa. Conserved hypothetical protein, equivalent to Q50200|ML2705|L222-ORF1 hypothetical protein from Mycobacterium leprae (259 aa),FASTA scores: opt: 1266, E(): 2e-74, (76.4% identity in 250 aa overlap). Also highly similar (but with gaps) to Q9R3S2|STH24.10 hypothetical 22.6 KDA protein from Streptomyces coelicolor (205 aa), FASTA scores: opt: 387,E(): 7.5e-18, (40.25% identity in 231 aa overlap). Predicted to be an outer membrane protein (See Song et al.,2008).

Thioredoxin TrxC (TRX) (MPT46); Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Rv3916c, (MTV028.07c), len: 244 aa. Conserved hypothetical protein, equivalent to Q50200|ML2705|L222-ORF1 hypothetical protein from Mycobacterium leprae (259 aa),FASTA scores: opt: 1266, E(): 2e-74, (76.4% identity in 250 aa overlap). Also highly similar (but with gaps) to Q9R3S2|STH24.10 hypothetical 22.6 KDA protein from Streptomyces coelicolor (205 aa), FASTA scores: opt: 387,E(): 7.5e-18, (40.25% identity in 231 aa overlap). Predicted to be an outer membrane protein (See Song et al.,2008).

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Conserved protein with FHA domain, FhaA; Regulates cell growth and peptidoglycan synthesis by binding to MviN. May inhibit the late stages of peptidoglycan synthesis.

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Probable UDP-N-acetylglucosamine 1-carboxyvinyltransferase MurA; Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine; Belongs to the EPSP synthase family. MurA subfamily.

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Possible resuscitation-promoting factor RpfA; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Stimulates growth of stationary phase M.bovis (a slow-growing Mycobacterium), reduces the lag phase of diluted fast-growers M.smegmatis and Micrococcus [...]

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Probable resuscitation-promoting factor RpfE; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Belongs to the transglycosylase family. Rpf subfamily.

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Rv3913, (MT4032, MTV028.04), len: 335 aa. Probable trxB2, thioredoxin reductase (see citation below),equivalent to O30973|TRXB_MYCSM thioredoxin reductase from Mycobacterium smegmatis (311 aa), FASTA scores: opt: 1575,E(): 1.8e-87, (78.35% identity in 305 aa overlap); and highly similar, but shorter at C-terminus, to P46843|TRXB_MYCLE|TRXB/a|TRX|ML2703 bifunctional thioredoxin reductase/thioredoxin from Mycobacterium leprae (458 aa), FASTA scores: opt: 1766, E(): 8.7e-99, (83.25% identity in 328 aa overlap). Also highly similar to many e.g. P52215|TRXB_STRCO|SCH24.12 from Streptomyces [...]

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Thioredoxin TrxC (TRX) (MPT46); Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.

Conserved protein with FHA domain, FhaA; Regulates cell growth and peptidoglycan synthesis by binding to MviN. May inhibit the late stages of peptidoglycan synthesis.

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Iron-dependent repressor and activator IdeR; Metal-dependent DNA-binding protein that controls transcription of many genes involved in iron metabolism. Acts as a repressor of siderophore biosynthesis and as a positive modulator of iron storage. Also regulates expression of transporters, proteins involved in siderophore synthesis, iron storage and transcriptional regulators.

Rv1049, (MTV017.02), len: 148 aa. Probable transcriptional repressor protein, similar to many e.g. P74870 negative regulator of EMR locus EMR from Salmonella typhimurium (149 aa), FASTA scores: opt: 146, E(): 0.0011,(31.6% identity in 95 aa overlap). Contains probable helix-turn-helix motif at aa 58-79 (Score 1495, +4.28 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).

Probable UDP-N-acetylglucosamine 1-carboxyvinyltransferase MurA; Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine; Belongs to the EPSP synthase family. MurA subfamily.

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Possible resuscitation-promoting factor RpfA; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Stimulates growth of stationary phase M.bovis (a slow-growing Mycobacterium), reduces the lag phase of diluted fast-growers M.smegmatis and Micrococcus [...]

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Possible resuscitation-promoting factor RpfA; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Stimulates growth of stationary phase M.bovis (a slow-growing Mycobacterium), reduces the lag phase of diluted fast-growers M.smegmatis and Micrococcus [...]

Probable resuscitation-promoting factor RpfE; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Belongs to the transglycosylase family. Rpf subfamily.

Probable resuscitation-promoting factor RpfE; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Belongs to the transglycosylase family. Rpf subfamily.

Probable peptidoglycan hydrolase; Cell-wall hydrolase that hydrolyzes the amide bond between N- acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to lyse whole mycobacteria, release peptidoglycan from the cell wall of M.luteus and M.smegmatis, and cleave N-acetylmuramoyl-L-alanyl-D- isoglutamine, releasing free N-acetylmuramic acid and dipeptide.

Probable resuscitation-promoting factor RpfE; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Belongs to the transglycosylase family. Rpf subfamily.

Possible resuscitation-promoting factor RpfA; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. Stimulates growth of stationary phase M.bovis (a slow-growing Mycobacterium), reduces the lag phase of diluted fast-growers M.smegmatis and Micrococcus [...]

Alternative RNA polymerase sigma-E factor (sigma-24) SigH (RPOE); Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by a cognate anti-sigma factor (RshA) until released. This sigma factor is involved in heat shock and oxidative stress responses; it positively regulates the expression of itself, sigE, sigB and a number of transcriptional regulators as well as other effectors of heat and oxidative stress, leading to direct and ind [...]

Rv3913, (MT4032, MTV028.04), len: 335 aa. Probable trxB2, thioredoxin reductase (see citation below),equivalent to O30973|TRXB_MYCSM thioredoxin reductase from Mycobacterium smegmatis (311 aa), FASTA scores: opt: 1575,E(): 1.8e-87, (78.35% identity in 305 aa overlap); and highly similar, but shorter at C-terminus, to P46843|TRXB_MYCLE|TRXB/a|TRX|ML2703 bifunctional thioredoxin reductase/thioredoxin from Mycobacterium leprae (458 aa), FASTA scores: opt: 1766, E(): 8.7e-99, (83.25% identity in 328 aa overlap). Also highly similar to many e.g. P52215|TRXB_STRCO|SCH24.12 from Streptomyces [...]

Alternative RNA polymerase sigma-E factor (sigma-24) SigH (RPOE); Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by a cognate anti-sigma factor (RshA) until released. This sigma factor is involved in heat shock and oxidative stress responses; it positively regulates the expression of itself, sigE, sigB and a number of transcriptional regulators as well as other effectors of heat and oxidative stress, leading to direct and ind [...]

Thioredoxin TrxC (TRX) (MPT46); Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.