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| | icd2 | Isocitrate dehydrogenase [NADP]; Rv0066c, (MTV030.09c), len: 745 aa. Probable icd2,isocitrate dehydrogenase NADP-dependent. Belongs to the monomeric-type family of IDH. Note that in H37Rv, Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2. (745 aa) |
| | sdaA | Rv0069c, (MTV030.12c), len: 461 aa. Probable sdaA,L-serine dehydratase. Belongs to the iron-sulfur dependent L-serine dehydratase family. Cofactor: iron-sulfur (4FE-4S) (probable). (461 aa) |
| | glyA2 | Serine hydroxymethyltransferase GlyA2 (serine methylase 2) (SHMT 2); Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of L- allo-threonine; Belongs to the SHMT family. (425 aa) |
| | Rv0075 | Rv0075, (MTV030.19), len: 390 aa. Probable aminotransferase, similar to many class-II pyridoxal-phosphate-dependent aminotransferases (MALY/PATB subfamily). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv2294, Rv0858c, etc. (390 aa) |
| | mtn | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase; Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'- methylthioribose and S-ribosylhomocysteine, respectively. (255 aa) |
| | ilvD | Rv0189c, (MTCI28.28c), len: 575 aa. Probable ilvD,dihydroxy-acid dehydratase, similar to many e.g. ILVD_LACLA|Q02139 dihydroxy-acid dehydratase (dad) from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (570 aa), FASTA scores: opt: 1605, E(): 0, (46.0% identity in 561 aa overlap). Also similar to ML2608|MLCL622.06c|O06069|ILVD_MYCLE dihydroxy-acid dehydratase from Mycobacterium leprae (564 aa). Contains PS00886 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1. Belongs to the ILVD / EDD family. Cofactor: binds 1 4FE-4S cluster (potential). (575 aa) |
| | aspC | Rv0337c, (MTCY279.04c), len: 429 aa. Probable aspC,aspartate aminotransferase (transaminase A), equivalent to CAC32019.1|AL583925 probable aspartate aminotransferase from Mycobacterium leprae (437 aa). Also highly similar to many e.g. Q48143|U32823 aspartate aminotransferase (404 aa), FASTA scores: opt: 1646, E(): 0, (57.2% identity in 404 aa overlap). Also some similarity to Rv3565|MTCY06G11.12 from Mycobacterium tuberculosis FASTA score: (27.2% identity in 383 aa overlap). Belongs to class-I of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. (429 aa) |
| | fba | Probable fructose-bisphosphate aldolase Fba; Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. (344 aa) |
| | Rv0428c | GCN5-related N-acetyltransferase; Rv0428c, (MTCY22G10.25c), len: 302 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. (302 aa) |
| | gpm1 | Probable phosphoglycerate mutase 1 Gpm1 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM); Catalyzes the interconversion of 2-phosphoglycerate and 3- phosphoglycerate; Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. (249 aa) |
| | proC | Probable pyrroline-5-carboxylate reductase ProC (P5CR) (P5C reductase); Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. (295 aa) |
| | serA2 | Rv0728c, (MTV041.02c), len: 326 aa. Possible serA2,D-3-phosphoglycerate dehydrogenase, similar to others e.g. AF0278|AF027868_5|YoaD D-3-phosphoglycerate dehydrogenase from Bacillus subtilis (344 aa), FASTA scores: opt: 594,E(): 3.1e-31, (35.9% identity in 309 aa overlap); etc. Also similar to Rv2996c|MTV012.10|SERA1 D-3-phosphoglycerate dehydrogenase from Mycobacterium tuberculosis (528 aa); Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. (326 aa) |
| | dapC | Probable N-succinyldiaminopimelate aminotransferase DapC (DAP-at); Involved in the lysine biosynthetic pathways. It catalyzes the transfer of an amino group from L-glutamate to N-succinyl-2-l- amino-6-oxoheptanedioate (N-succinyl-2-l-amino-6-ketopimelate) in a PLP-dependent reaction, yielding as products N-succinyl-l-2,6- diaminoheptanedioate (N-succinyl-diaminopimelate) and 2-oxoglutarate (Probable); Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. (397 aa) |
| | serC | Possible phosphoserine aminotransferase SerC (PSAT); Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. (376 aa) |
| | citA | Probable citrate synthase II CitA; Rv0889c, (MTCY31.17c), len: 373 aa. Probable citA (alternate gene name: gltA), citrate synthase 2, highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30,(33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. (373 aa) |
| | gltA2 | Rv0896, (MTCY31.24), len: 431 aa. Probable gltA2,citrate synthase 1, highly similar to O33066|NP_302405.1|NC_002677 citrate synthase 1 from Mycobacterium leprae (431 aa), FASTA scores: E(): 0, (91.0 identity in 431 aa overlap); and AAF04133.1|AF191033_1|AF191033 citrate synthase from Mycobacterium smegmatis (441 aa). Also highly similar to others e.g. AAF14286.1|AF181118_1|AF181118 citrate synthase from Streptomyces coelicolor (429 aa); P42457|CISY_CORGL citrate synthase from Corynebacterium glutamicum (437 aa),FASTA scores: opt: 1847, E(): 0, (63.0% identity in 433 aa overlap); etc. A [...] (431 aa) |
| | Rv0948c | Probable mycolyl transferase, pseudogene; Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis. (105 aa) |
| | prsA | Ribose-phosphate pyrophosphokinase; Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) and of the decaprenylphosphoryl-arabinose (DPA), an essential precursor for the mycobacterial cell wall biosynthesis. Catalyzes the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P) to yield phosphoribosyl diphosphate (PRPP) and AMP. It can also use GTP, CTP and UTP as diphosphoryl donors. (326 aa) |
| | eno | Probable enolase Eno; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis; Belongs to the enolase family. (429 aa) |
| | cbs | Probable cystathionine beta-synthase Cbs (serine sulfhydrase) (beta-thionase) (hemoprotein H-450); Rv1077, (MTV017.30), len: 464 aa. Probable cbs (previously cysM2), cystathionine beta-synthase, similar throughout its length to many eukaryotic cystathionine beta-synthases e.g. P32232|CBS_RAT cystathionine beta-synthase (560 aa), FASTA scores: opt: 951, E(): 0,(40.2% identity in 450 aa overlap); also similar in N-terminal domain (aa 1 - 330) to Rv2334|MTCY98.03 CysK Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 855, E(): 0, (46.8% identity in 314 overlap); and other cysteine s [...] (464 aa) |
| | metB | Cystathionine gamma-synthase MetB (CGS) (O-succinylhomoserine [thiol]-lyase); Catalyzes the formation of L-cystathionine from O-succinyl-L- homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction (By similarity). In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia. (388 aa) |
| | glyA1 | Serine hydroxymethyltransferase 1 GlyA1; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of L- allo-threonine; Belongs to the SHMT family. (426 aa) |
| | prpC | Probable methylcitrate synthase PrpC; Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the Claisen condensation of propionyl-CoA and oxaloacetate (OAA) to yield 2-methylcitrate (2-MC) and CoA. Also catalyzes the condensation of oxaloacetate with acetyl-CoA. (393 aa) |
| | metE | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. (759 aa) |
| | dapD | Tetrahydrodipicolinate N-succinyltransferase DapD; Catalyzes the conversion of the cyclic tetrahydrodipicolinate (THDP) into the acyclic N-succinyl-L-2-amino-6-oxopimelate using succinyl-CoA. (317 aa) |
| | dapE | Probable succinyl-diaminopimelate desuccinylase DapE; Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid. (354 aa) |
| | lysA | Diaminopimelate decarboxylase LysA (DAP decarboxylase); Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine (Probable). Is essential for the viability of M.tuberculosis in the host. (447 aa) |
| | thrA | Rv1294, (MTCY373.14), len: 441 aa. Probable thrA (hom), homoserine dehydrogenase, highly similar to DHOM_MYCLE|P46806 from Mycobacterium leprae (441 aa), FASTA scores: opt: 2437, E():0, (89.5% identity in 438 aa overlap). Contains PS00017 ATP/GTP-binding site motif A; PS01042 Homoserine dehydrogenase signature. Belongs to the homoserine dehydrogenase family. (441 aa) |
| | thrC | Threonine synthase ThrC (ts); Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine. (360 aa) |
| | thrB | Probable homoserine kinase ThrB; Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate. (316 aa) |
| | metK | S-adenosylmethionine synthase; Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. (403 aa) |
| | rpe | Probable ribulose-phosphate 3-epimerase Rpe (PPE) (R5P3E) (pentose-5-phosphate 3-epimerase); Catalyzes the reversible epimerization of D-ribulose 5- phosphate to D-xylulose 5-phosphate; Belongs to the ribulose-phosphate 3-epimerase family. (232 aa) |
| | gap | Probable glyceraldehyde 3-phosphate dehydrogenase Gap (GAPDH); Catalyzes the oxidative phosphorylation of glyceraldehyde 3- phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG; Belongs to the glyceraldehyde-3-ph [...] (339 aa) |
| | pgk | Rv1437, (MTCY493.17c), len: 412 aa. Probable pgk,Phosphoglycerate kinase, highly similar to many e.g. PGK_MYCLE|P46712 Mycobacterium leprae (416 aa), FASTA scores: opt: 2153, E(): 0, (80.4% identity in 414 aa overlap). Contains PS00111 Phosphoglycerate kinase signature. Belongs to the phosphoglycerate kinase family. (412 aa) |
| | tpi | Probable triosephosphate isomerase Tpi (TIM); Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D- glyceraldehyde-3-phosphate (G3P); Belongs to the triosephosphate isomerase family. (261 aa) |
| | tal | Probable transaldolase Tal; Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway; Belongs to the transaldolase family. Type 2 subfamily. (373 aa) |
| | tkt | Transketolase Tkt (TK); Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Belongs to the transketolase family. (700 aa) |
| | acn | Probable iron-regulated aconitate hydratase Acn (citrate hydro-lyase) (aconitase); Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. The apo form of AcnA functions as a RNA-binding regulatory protein which binds to selected IRE-like sequences present within the UTRs (untranslated regions) of 3' trxC and 5' IdeR mRNA. Could catalyze the hydration of 2-methyl-ci [...] (943 aa) |
| | ilvA | Probable threonine dehydratase IlvA; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA (By similarity). (429 aa) |
| | hisD | Probable histidinol dehydrogenase HisD (HDH); Catalyzes the sequential NAD-dependent oxidations of L- histidinol to L-histidinaldehyde and then to L-histidine. (438 aa) |
| | hisC1 | Rv1600, (MTCY336.04c), len: 380 aa. Probable hisC1,histidinol-phosphate aminotransferase O06591. Similar to many e.g. HIS8_STRCO|P16246 from Streptomyces coelicolor (369 aa), FASTA results: opt: 1353, E(): 0, (59.0% identity in 356 aa overlap). Some similarity to other Mycobacterium tuberculosis aminotransferases e.g. Rv3772|MTCY13D12.06,FASTA results: E(): 7.4e-25, (33.7% identity in 365 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. Note that previously known as hisC. (380 aa) |
| | hisB | Rv1601, (MTCY336.03c), len: 210 aa. Probable hisB,imidazole glycerol-phosphate dehydratase. Similar to many e.g. HIS7_STRCO|P16247 from Streptomyces coelicolor (197 aa),FASTA results: opt: 763, E(): 0, (57.4% identity in 202 aa overlap). Belongs to the imidazoleglycerol-phosphate dehydratase family. (210 aa) |
| | hisH | Probable amidotransferase HisH; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF (By similarity). (206 aa) |
| | hisA | Probable phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase HisA; Involved in both the histidine and tryptophan biosynthetic pathways; Belongs to the HisA/HisF family. (245 aa) |
| | hisF | Probable cyclase HisF; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit (By similarity). (267 aa) |
| | hisI | Probable phosphoribosyl-AMP 1,6 cyclohydrolase HisI; Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP. (115 aa) |
| | trpE | Anthranilate synthase component I TrpE (glutamine amidotransferase); Part of a heterotetrameric complex that catalyzes the two- step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly [...] (516 aa) |
| | trpC | Rv1611, (MTCY01B2.03), len: 272 aa. Probable trpC,indole-3-glycerol phosphate synthase. Similar to Q55508|SLR0546 hypothetical 33.0 kDa protein from synechocystis SP (295 aa), FASTA score: opt: 26, E(): 7.6e-32, (44.2% identity in 265 aa overlap); also similar to TRPC_AZOBR|P26938 ndole-3-glycerol-phosphate synthaseindole-3-glycerol-phosphate synthase from Azospirillum brasilense (262 aa), FASTA score: opt: 596,E(): 4.8e-30, (43.8% identity in 258 aa overlap). Equivalent to AL0499 13|MLCB1610_24 from Mycobacterium leprae (272 aa) (90.8% identity in 272 aa overlap). Contains indole-3-gl [...] (272 aa) |
| | trpB | Tryptophan synthase, beta subunit TrpB; The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine. (410 aa) |
| | trpA | Probable tryptophan synthase, alpha subunit TrpA; The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. (270 aa) |
| | pykA | Rv1617, (MTCY01B2.09), len: 472 aa. Probable pykA,pyruvate kinase. FASTA best: Q46078 pyruvate kinase from corynebacterium glutamicum (475 aa), opt: 2221, E(): 0,(72.2% identity in 468 aa overlap). Belongs to the pyruvate kinase family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). (472 aa) |
| | argC | Probable N-acetyl-gamma-glutamyl-phoshate reductase ArgC; Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. (352 aa) |
| | argJ | Probable glutamate N-acetyltransferase ArgJ; Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate. (404 aa) |
| | argB | Probable acetylglutamate kinase ArgB; Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate. (294 aa) |
| | argD | Rv1655, (MTCY06H11.20), len: 400 aa. Probable argD,Acetylornithine aminotransferase, similar to ARGD_ECOLI|P18335 (406 aa), FASTA scores: opt: 958, E(): 0,(38.6% identity in 404 aa overlap), contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. (400 aa) |
| | argF | Probable ornithine carbamoyltransferase, anabolic ArgF; Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis; Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family. (307 aa) |
| | argG | Rv1658, (MTCY06H11.23), len: 398 aa. Probable argG,Argininosuccinate synthase, similar to ASSY_STRCL|P50986 argininosuccinate synthase from Streptomyces clavuligerus (397 aa), FASTA scores: opt: 1873, E(): 0, (67.8% identity in 397 aa overlap); contains PS00564 Argininosuccinate synthase signature 1, PS00565 Argininosuccinate synthase signature 2. Belongs to the argininosuccinate synthase family. (398 aa) |
| | argH | Rv1659, (MTCY06H11.24), len: 470 aa. Probable argH,Argininosuccinate lyase, similar to ARLY_ECOLI|P11447 argininosuccinate lyase from Escherichia coli (457 aa),FASTA scores: opt: 1091, E(): 0, (42.5% identity in 461 aa overlap); contains PS00017 ATP/GTP-binding site motif A,PS00163 Fumarate lyases signature. Belongs to the lyase 1 family. Argininosuccinate lyase subfamily. (470 aa) |
| | ilvG | Probable acetolactate synthase IlvG (acetohydroxy-acid synthase)(ALS); Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. (547 aa) |
| | glnA3 | Rv1878, (MTCY180.40c), len: 450 aa. Probable glnA3,glutamine synthetase class I, similar to many e.g. GLNA_BACCE|P19064 from Bacillus cereus (443 aa), FASTA results: opt: 497, E(): 5.2e-23, (29.0% identity in 331 aa overlap); etc. Also similar to C-terminus of FLUG_EMENI|P38094 flug protein from emericella nidulans (865 aa), FASTA scores: opt: 227, E (): 6.4e-13, (29.9% identity in 394 aa overlap). Note that the downstream ORF MTCY180.39c is similar to the N-terminus. Also similar to three other potential glutamine synthases in M. tuberculosis: Q10378|GLN2_MYCTU|GLNA2|Rv2222c|MT2280|MT [...] (450 aa) |
| | Rv1885c | Chorismate mutase; Catalyzes the Claisen rearrangement of chorismate to prephenate. May play some role in the pathogenicity. (199 aa) |
| | pfkB | 6-phosphofructokinase PfkB (phosphohexokinase) (phosphofructokinase); Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. (339 aa) |
| | hisG | ATP phosphoribosyltransferase HisG; Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity (By similarity). (284 aa) |
| | hisE | Phosphoribosyl-AMP pyrophosphatase HisE; Rv2122c, (MTCY261.18), len: 93 aa. HisE (alternate gene name: irg1), phosphoribosyl-AMP cyclohydrolase (see citation below), similar to many. Note that previously misnamed hisI. (93 aa) |
| | metH | Methionine synthase; Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate (By similarity); Belongs to the vitamin-B12 dependent methionine synthase family. (1192 aa) |
| | aroG | Phospho-2-dehydro-3-deoxyheptonate aldolase AroG; Catalyzes an aldol-like condensation reaction between phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate (E4P) to generate 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P) and inorganic phosphate. (462 aa) |
| | trpD | Probable anthranilate phosphoribosyltransferase TrpD; Catalyzes the transfer of the phosphoribosyl group of 5- phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'- phosphoribosyl)-anthranilate (PRA). (370 aa) |
| | asnB | Rv2201, (MTCY190.12), len: 652 aa. Probable asnB,asparagine synthetase, similar to e.g. SW:ASNH_BACSU P42113 putative asparagine synthetase (26.0% identity in 438 aa overlap). (652 aa) |
| | ilvE | Branched-chain amino acid transaminase IlvE; Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to their efficiency as amino donor: Leu > Ile > Val. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. (368 aa) |
| | glnA1 | Glutamine synthetase GlnA1 (glutamine synthase) (GS-I); Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Also able to use GTP. D-glutamate is a poor substrate, and DL-glutamate shows about 50% of the standard specific activity. Also plays a key role in controlling the ammonia levels within infected host cells and so contributes to the pathogens capacity to inhibit phagosome acidification and phagosome-lysosome fusion. Involved in cell wall biosynthesis via the production of the major component p [...] (478 aa) |
| | glnA2 | Probable glutamine synthetase GlnA2 (glutamine synthase) (GS-II); Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism. It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Feedback- inhibited GlnA also interacts with and regulates the activity of the transcriptional regulator TnrA. During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of gene [...] (446 aa) |
| | cobC | Rv2231c, (MTCY427.12c), len: 364 aa. Possible cobC,aminotransferase. Note that initiation codon uncertain. Similar to CobC aminotransferases e.g. sp|P21633|COBC_PSEDE COBC protein (333 aa) opt: 277, E(): 1.7e-11; 28.8% identity in 313 aa overlap and also to e.g. SW:HIS8_ECOLI P06986 histidinol-phosphate aminotransferase (27.0% identity in 289 aa overlap), contains PS00105 aminotransferases class-I pyridoxal-phosphate attachment site. Real Mycobacterium tuberculosis histidinol-phosphate aminotransferase, hisC, is Rv1600 (MTCY336.04c); Belongs to the class-I pyridoxal-phosphate-dependent [...] (364 aa) |
| | Rv2294 | Rv2294, (MTCY339.16c), len: 407 aa. Probable aminotransferase, similar to others in M. tuberculosis e.g. MTV030_19, also similar to PATB_BACSU|Q08432 putative aminotransferase b from Bacillus subtilis (387 aa), FASTA scores: opt: 563, E(): 2.8e-29, (31.4% identity in 408 aa overlap); and to MALY_ECOLI|P23256 maly protein from Escherichia coli (390 aa), FASTA scores: opt: 530, E(): 3.6e-27, (31.3% identity in 384 aa overlap). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. (407 aa) |
| | cysK1 | O-acetylserine sulfhydrylase; Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide. Belongs to the cysteine synthase/cystathionine beta- synthase family. (310 aa) |
| | cysE | Probable serine acetyltransferase CysE (sat); Catalyzes the acetylation of serine by acetyl-CoA to produce O-acetylserine (OAS); Belongs to the transferase hexapeptide repeat family. (229 aa) |
| | proA | Gamma-glutamyl phosphate reductase; Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. Belongs to the gamma-glutamyl phosphate reductase family. (415 aa) |
| | proB | Probable glutamate 5-kinase protein ProB (gamma-glutamyl kinase) (GK); Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate. (376 aa) |
| | rpiB | Ribose-5-phosphate isomerase; Catalyzes the interconversion of ribulose-5-P and ribose-5-P. It has not isomerase activity towards D-allose 6-phosphate. (162 aa) |
| | aroD | 3-dehydroquinate dehydratase AroD (AROQ) (3-dehydroquinase) (type II dhqase); Catalyzes a trans-dehydration via an enolate intermediate. (147 aa) |
| | aroB | 3-dehydroquinate synthase AroB; Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ). (362 aa) |
| | aroK | Shikimate kinase AroK (SK); Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. Belongs to the shikimate kinase family. (176 aa) |
| | aroF | Probable chorismate synthase AroF (5-enolpyruvylshikimate-3-phosphate phospholyase); Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system. (401 aa) |
| | aroE | Rv2552c, (MTCY159.04), len: 269 aa. Probable aroE,shikimate 5-dehydrogenase, equivalent to Q9CCS7|AROE|ML0515 putative shikimate 5-dehydrogenase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1452, E(): 1.8e-77,(81.5% identity in 270 aa overlap). Also highly similar,but longer 101 aa, to Q9KH59|AROE putative shikimate dehydrogenase (fragment) from Mycobacterium marinum (148 aa), FASTA scores: opt: 729, E(): 1.3e-35, (76.35% identity in 148 overlap); Q9F7W3|AROE from Mycobacterium ulcerans (148 aa), FASTA scores: opt: 718, E(): 5.9e-35, (75.7% identity in 148 aa overlap). And al [...] (269 aa) |
| | dapF | Probable diaminopimelate epimerase DapF (DAP epimerase); Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L- lysine and an essential component of the bacterial peptidoglycan. (289 aa) |
| | argA | Probable L-glutamate alpha-N-acetyltranferase ArgA (alpha-N-acetylglutamate synthase); Catalyzes the conversion of L-glutamate to alpha-N-acetyl-L- glutamate. L-glutamine is a significantly better substrate compared to L-glutamate; Belongs to the acetyltransferase family. (174 aa) |
| | dapA | Probable dihydrodipicolinate synthase DapA (DHDPS) (dihydrodipicolinate synthetase); Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA). Belongs to the DapA family. (300 aa) |
| | dapB | Dihydrodipicolinate reductase DapB (DHPR); Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate (Probable). Can use both NADH and NADPH as a reductant, with NADH being 6-fold as effective as NADPH. (245 aa) |
| | glnA4 | Rv2860c, (MTV003.06c), len: 457 aa. Probable glnA4,glutamine synthetase class II, similar to many glutamine synthases e.g. O88070|SCI35.35c from Streptomyces coelicolor (462 aa), FASTA scores: opt: 1947, E(): 8.2e-120, (64.15% identity in 452 aa overlap); Q98H15|MLL3074 from Rhizobium loti (Mesorhizobium loti) (465 aa), FASTA scores: opt: 1321, E(): 7.8e-79, (46.7% identity in 452 aa overlap); Q98EM0|MLL4187 from Rhizobium loti (Mesorhizobium loti) (456 aa), FASTA scores: opt: 698,E(): 4.6e-38, (33.5% identity in 454 aa overlap); Q9CDL9|GLNA from Lactococcus lactis (subsp. lactis) (Str [...] (457 aa) |
| | pca | Probable pyruvate carboxylase Pca (pyruvic carboxylase); Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. (1127 aa) |
| | leuD | 3-isopropylmalate dehydratase small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. (198 aa) |
| | leuC | 3-isopropylmalate dehydratase large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. (473 aa) |
| | leuB | Probable 3-isopropylmalate dehydrogenase LeuB (beta-IPM dehydrogenase) (IMDH) (3-IPM-DH); Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate; Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily. (336 aa) |
| | serA1 | Probable D-3-phosphoglycerate dehydrogenase SerA1 (PGDH); Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L- serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate. (528 aa) |
| | ilvC | Ketol-acid reductoisomerase (NADP(+)); Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. It is also able to use 3-hydroxypyruvate (HP). (333 aa) |
| | ilvN | Probable acetolactate synthase (small subunit) IlvN (acetohydroxy-acid synthase) (AHAS) (ALS); Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Belongs to the acetolactate synthase small subunit family. (168 aa) |
| | ilvB1 | Acetolactate synthase (large subunit) IlvB1 (acetohydroxy-acid synthase); Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-aceto-2- hydroxybutyrate. (618 aa) |
| | pfkA | Probable 6-phosphofructokinase PfkA (phosphohexokinase) (phosphofructokinase); Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis; Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily. (343 aa) |
| | serB2 | Probable phosphoserine phosphatase SerB2 (PSP) (O-phosphoserine phosphohydrolase) (pspase); Catalyzes the dephosphorylation of O-phospho-L-serine into L- serine, a step in the L-serine biosynthetic pathway. Exhibits high specificity for L-phosphoserine compared to substrates like L-phosphothreonine (5% relative activity) and L-phosphotyrosine (1.7% relative activity). Belongs to the HAD-like hydrolase superfamily. SerB family. (409 aa) |
| | hisN | Probable monophosphatase; Catalyzes the dephosphorylation of histidinol-phosphate to histidinol, the direct precursor of histidine; Belongs to the inositol monophosphatase superfamily. (260 aa) |
| | aroA | 3-phosphoshikimate 1-carboxyvinyltransferase; Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. (450 aa) |
| | icd1 | Isocitrate dehydrogenase [NADP]; Rv3339c, (MTV016.39c), len: 409 aa. Probable icd1,isocitrate dehydrogenase NADP-dependent, highly similar to many e.g. Q9A5C8|CC2522 from Caulobacter crescentus (403 aa), FASTA scores: opt: 1972, E(): 4.6e-115, (72.45% identity in 403 aa overlap); AAF73472|ICD from Rhizobium meliloti (404 aa), FASTA scores: opt: 1968, E(): 8.1e-115,(73.2% identity in 403 aa overlap); P50215|IDH_SPHYA from Sphingomonas yanoikuyae (406 aa), FASTA scores: opt: 1964,E(): 1.4e-114, (71.45% identity in 403 aa overlap); etc. Contains PS00470 Isocitrate and isopropylmalate dehy [...] (409 aa) |
| | metA | Homoserine O-acetyltransferase; Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine. (379 aa) |
| | ilvB2 | Putative acetolactate synthase large subunit IlvB2; Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Belongs to the TPP enzyme family. (552 aa) |
| | ilvX | Probable acetohydroxyacid synthase IlvX (acetolactate synthase); Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. (515 aa) |
| | Rv3684 | Probable lyase; Rv3684, (MTV025.032), len: 346 aa. Probable lyase, and more specifically a cysteine synthase, highly similar to many lyases e.g. Q9K3N2|SCG20A.08c putative lyase from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1469,E(): 3.7e-85, (63.35% identity in 341 aa overlap) (shorter 31 aa at N-terminus); Q9KT44|VC1061 cysteine synthase/ cystathionine beta-synthase family protein from Vibrio cholerae (355 aa), FASTA scores: opt: 1366, E(): 1.1e-78,(63.25% identity in 321 aa overlap); Q9I4R3|PA1061 hypothetical protein from Pseudomonas aeruginosa (365 aa),FASTA scores: op [...] (346 aa) |
| | asd | Aspartate-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate. Is essential for the growth and pathogenicity of M.tuberculosis, and for the generation of the bacterial cell wall ; Belongs to the aspartate-semialdehyde dehydrogenase family. (345 aa) |
| | ask | Aspartokinase Ask (aspartate kinase) [contains: aspartokinase alpha subunit (Ask-alpha); Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids lysine, threonine, isoleucine and methionine; Belongs to the aspartokinase family. (421 aa) |
| | leuA | 2-isopropylmalate synthase LeuA (alpha-isopropylmalate synthase) (alpha-IPM synthetase) (IPMS); Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate). (644 aa) |
| | tyrA | Prephenate dehydrogenase TyrA (PDH) (hydroxyphenylpyruvate synthase); Catalyzes the NAD(+)-dependent conversion of prephenate to p- hydroxyphenylpyruvate, with the elimination of carbon dioxide. Is a key regulatory enzyme in tyrosine biosynthesis. Displays no chorismate mutase (CM) activity, in contrast to TyrA from E.coli and some other bacteria, that are bifunctional and possess a CM domain. (301 aa) |
| | hisC2 | Putative phenylalanine aminotransferase; May catalyze the transamination reaction in phenylalanine biosynthesis; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. (353 aa) |
| | pheA | Rv3838c, (MTCY01A6.31), len: 321 aa. PheA,prephenate dehydratase (see citation below), equivalent to Q9CDC4|PHEA|ML0078 putative prephenate dehydratase from Mycobacterium leprae (322 aa), FASTA scores: opt: 1690,E(): 1.3e-93, (84.25% identity in 311 aa overlap). Also highly similar to others e.g. P10341|PHEA_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (315 aa), FASTA scores: opt: 843, E(): 4e-43, (45.8% identity in 308 aa overlap); Q9ZBX0|SCD78.29c from Streptomyces coelicolor (310 aa), FASTA scores: opt: 820, E(): 9.2e-42,(46.45% identity in 312 aa overlap); Q44104|P [...] (321 aa) |
| | gltD | Glutamate synthase [NADPH] small chain; Rv3858c, (MTCY01A6.10), len: 488 aa. Probable gltD,small subunit of NADH-dependent glutamate synthase,equivalent to Q9CDD4|GLTD|ML0062 NADH-dependent glutamate synthase small subunit from Mycobacterium leprae (488 aa),FASTA scores: opt: 2997, E(): 1e-166, (87.7% identity in 488 aa overlap). Also highly similar to many e.g. Q9S2Z0|SC3A3.03s from Streptomyces coelicolor (487 aa),FASTA scores: opt: 2152, E(): 1.2e-117, (63.85% identity in 487 aa overlap); Q9KPJ3|VC2374 from Vibrio cholerae (489 aa), FASTA scores: opt: 1699, E(): 2.5e-91, (51.75% ide [...] (488 aa) |
| | gltB | Glutamate synthase [NADPH] large chain; Rv3859c, (MTCY01A6.09), len: 1527 aa. Probable gltB,ferredoxin-dependent glutamate synthase large subunit,equivalent to Q9CDD5|GLTB|ML0061 putative ferredoxin-dependent glutamate synthase from Mycobacterium leprae (1527 aa), FASTA scores: opt: 9277, E(): 0, (90.25% identity in 1527 aa overlap). Also highly similar to many e.g. Q9S2Y9|SC3A3.04c from Streptomyces coelicolor (1514 aa), FASTA scores: opt: 5939, E(): 0, (64.3% identity in 1544 aa overlap); Q9Z465|GLTB from Corynebacterium glutamicum (Brevibacterium flavum) (1510 aa), FASTA scores: opt [...] (1527 aa) |
