Your Input: | |
| | lasT | Putative tRNA/tRNA methyltransferase; Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA. (228 aa) |
| | serS | Serine tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (430 aa) |
| | STM0901 | Fels-1 putative prophage DNA primase. (322 aa) |
| | STM4549 | Putative cytoplasmic protein. (152 aa) |
| | ybjF | Putative tRNA (uracil-5-)-methyltransferase; Catalyzes the formation of 5-methyl-uridine at position 747 (m5U747) in 23S rRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. RlmC subfamily. (376 aa) |
| | ybiN | Putative SAM-dependent methyltransferase; Specifically methylates the adenine in position 1618 of 23S rRNA. (308 aa) |
| | rhlE | Putative ATP-dependent RNA helicase; DEAD-box RNA helicase involved in ribosome assembly. Has RNA- dependent ATPase activity and unwinds double-stranded RNA. (454 aa) |
| | glnS | Similar to E. coli glutamine tRNA synthetase (AAC73774.1); Blastp hit to AAC73774.1 (554 aa), 96% identity in aa 1 - 554. (555 aa) |
| | ybeY | Putative metal-dependent hydrolase; Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. (157 aa) |
| | STM0650 | Similar to E. coli putative hydrolase (AAC76162.1); Blastp hit to AAC76162.1 (523 aa), 35% identity in aa 167 - 521, 32% identity in aa 119 - 223. (390 aa) |
| | leuS | Similar to E. coli leucine tRNA synthetase (AAC73743.1); Blastp hit to AAC73743.1 (860 aa), 95% identity in aa 1 - 860; Belongs to the class-I aminoacyl-tRNA synthetase family. (860 aa) |
| | ycbY | Putative N6-adenine-specific DNA methylase; Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA. Belongs to the methyltransferase superfamily. RlmKL family. (702 aa) |
| | yccW | Putative SAM-dependent methyltransferase; Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA. (403 aa) |
| | STM1109 | Putative periplasmic protein; Similar to E. coli orf, hypothetical protein (AAC75328.1); Blastp hit to AAC75328.1 (311 aa), 27% identity in aa 7 - 147, 27% identity in aa 155 - 277. (312 aa) |
| | rne | RNase E; Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs. Belongs to the RNase E/G family. RNase E subfamily. (1067 aa) |
| | ybeA | Putative cytoplasmic protein; Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA; Belongs to the RNA methyltransferase RlmH family. (155 aa) |
| | rna | Similar to E. coli RNase I, cleaves phosphodiester bond between any two nucleotides (AAC73712.1); Blastp hit to AAC73712.1 (268 aa), 73% identity in aa 1 - 268; Belongs to the RNase T2 family. (268 aa) |
| | cysS | Similar to E. coli cysteine tRNA synthetase (AAC73628.1); Blastp hit to AAC73628.1 (461 aa), 94% identity in aa 1 - 461; Belongs to the class-I aminoacyl-tRNA synthetase family. (461 aa) |
| | ybbB | Putative ATPase; Involved in the post-transcriptional modification of the uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Catalyzes the conversion of 2-thiouridine (S2U-RNA) to 2-selenouridine (Se2U-RNA). Acts in a two-step process involving geranylation of 2-thiouridine (S2U) to S-geranyl-2-thiouridine (geS2U) and subsequent selenation of the latter derivative to 2-selenouridine (Se2U) in the tRNA chain (Probable); Belongs to the SelU family. (364 aa) |
| | ybaK | Putative cytoplasmic protein; Functions in trans to edit the amino acid from incorrectly charged Cys-tRNA(Pro) via a Cys-tRNA(Pro) deacylase activity. (159 aa) |
| | thiI | Sulfur transfer protein (from cys to ThiS and from IscS to U8-tRNA); Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. (482 aa) |
| | tgt | tRNA-guanine transglycosylase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the [...] (375 aa) |
| | queA | S-adenosylmethionine-tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). (354 aa) |
| | rnhA | RNase HI; Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. (155 aa) |
| | yaeB | Putative regulator; Similar to E. coli orf, hypothetical protein (AAC73306.1); Blastp hit to AAC73306.1 (235 aa), 89% identity in aa 1 - 235. (235 aa) |
| | proS | Proline tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves de [...] (572 aa) |
| | yaeJ | putative-tRNA hydrolase domain protein; Similar to E. coli orf, hypothetical protein (AAC73302.1); Blastp hit to AAC73302.1 (140 aa), 85% identity in aa 1 - 136. (140 aa) |
| | rnhB | RNAse HII; Endonuclease that specifically degrades the RNA of RNA-DNA hybrids; Belongs to the RNase HII family. (198 aa) |
| | ligT | 2'-5' RNA ligase; Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'- phosphomonoester; Belongs to the 2H phosphoesterase superfamily. ThpR family. (176 aa) |
| | yadB | Putative glutamyl t-RNA synthetase; Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2- cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon; Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily. (313 aa) |
| | yabC | Putative S-adenosyl methionine adenyltransferase; Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. (313 aa) |
| | rluA | 23S rRNA pseudouridylate 746 synthase; Dual specificity enzyme that catalyzes the synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs. (219 aa) |
| | ksgA | S-adenosylmethionine-6-N',N'-adenosyl (rRNA) dimethyltransferase; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. (273 aa) |
| | ileS | Isoleucine tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (944 aa) |
| | dinG | LexA regulated (SOS) repair enzyme; DNA-dependent ATPase and 5'-3' DNA helicase. (714 aa) |
| | smtA | S-adenosylmethionine-dependent methyltransferase; Catalyzes the methylation of 5-carboxymethoxyuridine (cmo5U) to form 5-methoxycarbonylmethoxyuridine (mcmo5U) at position 34 in tRNAs; Belongs to the class I-like SAM-binding methyltransferase superfamily. CmoM family. (267 aa) |
| | rsmC | 16S ribosomal rRNA; Specifically methylates the guanine in position 1207 of 16S rRNA in the 30S particle; Belongs to the methyltransferase superfamily. RsmC family. (342 aa) |
| | asnS | Similar to E. coli asparagine tRNA synthetase (AAC74016.1); Blastp hit to AAC74016.1 (466 aa), 94% identity in aa 1 - 466. (466 aa) |
| | yjiW | Putative SOS response protein; Involved in the degradation and recycling of damaged RNA. It is itself a target for degradation by the ATP-dependent protease Lon. Belongs to the SymE family. (110 aa) |
| | trpS2 | Putative tryptophanyl-tRNA synthetase; Similar to E. coli tryptophan tRNA synthetase (AAC76409.1); Blastp hit to AAC76409.1 (334 aa), 26% identity in aa 2 - 329; Belongs to the class-I aminoacyl-tRNA synthetase family. (337 aa) |
| | valS | Valine tRNA synthetase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (951 aa) |
| | miaE | Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA; Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen- dependent transformation of MS[2]I[6]A into MS[2]IO[6]A. (270 aa) |
| | STM4446 | Putative selenocysteine synthase; [L-seryl-tRNA(Ser) selenium transferase]. (372 aa) |
| | rluC | 23S rRNA pseudouridylate synthase; Responsible for synthesis of pseudouridine from uracil at positions 955, 2504 and 2580 in 23S ribosomal RNA; Belongs to the pseudouridine synthase RluA family. (319 aa) |
| | ymfC | Putative ribosomal large subunit pseudouridine synthase; Responsible for synthesis of pseudouridine from uracil-2457 in 23S ribosomal RNA; Belongs to the pseudouridine synthase RsuA family. (221 aa) |
| | yeaK | Putative cytoplasmic protein; Similar to E. coli orf, hypothetical protein (AAC74857.1); Blastp hit to AAC74857.1 (167 aa), 87% identity in aa 1 - 165. (172 aa) |
| | ydiZ | Putative cytoplasmic protein; Similar to E. coli orf, hypothetical protein (AAC74794.1); Blastp hit to AAC74794.1 (96 aa), 69% identity in aa 1 - 94. (96 aa) |
| | thrS | Threonine tRNA synthetase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (642 aa) |
| | pheS | Similar to E. coli phenylalanine tRNA synthetase, alpha-subunit (AAC74784.1); Blastp hit to AAC74784.1 (327 aa), 97% identity in aa 1 - 327. (327 aa) |
| | pheT | Phenylalanine tRNA synthetase, beta-subunit; phenylalanyl-tRNA synthetase beta chain. (SW:SYFB_SALTY); Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (795 aa) |
| | rnt | RNase T; Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis. (215 aa) |
| | tyrS | Tyrosine tRNA synthetase; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. (424 aa) |
| | STM1548 | Putative S-adenosylmethionine:tRNA-ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). (346 aa) |
| | hrpA | Similar to E. coli helicase, ATP-dependent (AAC74495.1); Blastp hit to AAC74495.1 (1281 aa), 95% identity in aa 1 - 1280. (1300 aa) |
| | dbpA | ATP-dependent RNA helicase; DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit. Has an RNA-dependent ATPase activity, which is specific for 23S rRNA, and a 3' to 5' RNA helicase activity that uses the energy of ATP hydrolysis to destabilize and unwind short rRNA duplexes. (457 aa) |
| | STM1665 | Putative cytoplasmic protein. (228 aa) |
| | rnb | RNase II; Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction. (644 aa) |
| | yciL | Putative ribosomal large subunit pseudouridine synthase; Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA; Belongs to the pseudouridine synthase RsuA family. (291 aa) |
| | trpH | trpR controlled transcriptional unit in the 5' upstream region of the trp operon; Efficiently catalyzes the hydrolysis of the 3'-phosphate from 3',5'-bis-phosphonucleotides as well as the successive hydrolysis of 5'-phosphomononucleotides from the 5'-end of short pieces of RNA and DNA, with no specificity toward the identity of the nucleotide base. Is more efficient at hydrolyzing RNA oligonucleotides than DNA oligonucleotides. This enzyme can also hydrolyze annealed DNA duplexes, albeit at a catalytic efficiency lower than that of the corresponding single-stranded oligonucleotides. (293 aa) |
| | hemA | Glutamyl tRNA reductase; Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA). (418 aa) |
| | pth | peptidyl-tRNA hydrolase; The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Involved in lambda inhibition of host protein synthesis. PTH activity may, directly or indirectly, be the target for lambda bar RNA leading to rap cell death (By similarity). (202 aa) |
| | rnd | RNase D; Exonuclease involved in the 3' processing of various precursor tRNAs. Initiates hydrolysis at the 3'-terminus of an RNA molecule and releases 5'-mononucleotides; Belongs to the RNase D family. (375 aa) |
| | yebU | Putative rRNA methyltransferase; Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA. (479 aa) |
| | aspS | Aspartate tRNA synthetase; Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp). Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (590 aa) |
| | yecP | Putative enzyme; Catalyzes carboxymethyl transfer from carboxy-S-adenosyl-L- methionine (Cx-SAM) to 5-hydroxyuridine (ho5U) to form 5- carboxymethoxyuridine (cmo5U) at position 34 in tRNAs. Belongs to the class I-like SAM-binding methyltransferase superfamily. CmoB family. (323 aa) |
| | argS | arginyl-tRNA synthetase. (SW:SYR_SALTY). (577 aa) |
| | fliA | Sigma F (sigma 28) factor of RNA polymerase; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor controls the expression of flagella-related genes. May regulate the expression of genes involved in virulence. (239 aa) |
| | sbcB | 3' --> 5' specific; deoxyribophosphodiesterase; similar to E. coli exonuclease I, 3' --> 5' specific; deoxyribophosphodiesterase (AAC75072.1); Blastp hit to AAC75072.1 (475 aa), 93% identity in aa 8 - 475. (476 aa) |
| | metG | Methionine tRNA synthetase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. (677 aa) |
| | yohI | Putative nitrogen regulation protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs. Belongs to the Dus family. DusC subfamily. (312 aa) |
| | rsuA | 16S rRNA pseudouridylate 516 synthase; Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA. (231 aa) |
| | alkB | DNA repair system specific for alkylated DNA; Dioxygenase that repairs alkylated DNA and RNA containing 3- methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha- ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents) (B [...] (216 aa) |
| | elaC | Putative metal-dependent hydrolase; Zinc phosphodiesterase, which has both exoribonuclease and endoribonuclease activities. (305 aa) |
| | truA | Pseudouridylate synthase I; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. (270 aa) |
| | mnmC | Putative peptidase; Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34; In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family. (686 aa) |
| | gltX | Glutamate tRNA synthetase, catalytic subunit; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). (471 aa) |
| | hisS | histidyl-tRNA synthetase. (SW:SYH_SALTY). (424 aa) |
| | yfgB | Putative Fe-S-cluster redox enzyme; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity; Belongs to the radical SAM superfamily. RlmN family. (388 aa) |
| | trmJ | Putative rRNA methylase; Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA. (243 aa) |
| | rnc | RNase III, ds RNA; Digests double-stranded RNA. Involved in the processing of ribosomal RNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Removes small helical intervening sequences (IVSs) from all 7 of the 23S rRNA transcripts. Probably also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. (226 aa) |
| | yfiC | Putative transferase; Specifically methylates the adenine in position 37 of tRNA(1)(Val) (anticodon cmo5UAC). (245 aa) |
| | srmB | ATP-dependent RNA helicase; DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit at low temperature. Exhibits RNA-stimulated ATP hydrolysis and RNA unwinding activity; Belongs to the DEAD box helicase family. SrmB subfamily. (444 aa) |
| | yfiF | Putative tRNA/rRNA methyltransferase; Similar to E. coli orf, hypothetical protein (AAC75634.1); Blastp hit to AAC75634.1 (345 aa), 87% identity in aa 1 - 345; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (345 aa) |
| | yfiP | Putative cytoplasmic protein; Similar to E. coli orf, hypothetical protein (AAC75636.1); Blastp hit to AAC75636.1 (240 aa), 88% identity in aa 9 - 234. (226 aa) |
| | rluD | Pseudouridine synthase; Responsible for synthesis of pseudouridine from uracil at positions 1911, 1915 and 1917 in 23S ribosomal RNA. (326 aa) |
| | trmD | tRNA (guanine-7-)-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (255 aa) |
| | alaS | alanyl-tRNA synthetase; Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain; Belongs to the class-II aminoacyl-tRNA synthetase family. (876 aa) |
| | ygbO | Putative hydrogenase subunit; Responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs; Belongs to the pseudouridine synthase TruD family. (349 aa) |
| | ygcB | Putative helicase; Similar to E. coli orf, hypothetical protein (AAC75803.1); Blastp hit to AAC75803.1 (888 aa), 30% identity in aa 7 - 849. (887 aa) |
| | ygcA | Putative RNA methyltransferase; Catalyzes the formation of 5-methyl-uridine at position 1939 (m5U1939) in 23S rRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. RlmD subfamily. (431 aa) |
| | yqcB | Putative synthase; Responsible for synthesis of pseudouridine from uracil-65 in transfer RNAs; Belongs to the pseudouridine synthase RluA family. (260 aa) |
| | ygdL | Similar to E. coli putative enzyme (AAC75854.1); Blastp hit to AAC75854.1 (268 aa), 93% identity in aa 1 - 267. (268 aa) |
| | ygdP | Putative invasion protein; Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage; Belongs to the Nudix hydrolase family. RppH subfamily. (176 aa) |
| | vapC | Putative nucleic acid-binding protein; Toxic component of a type II toxin-antitoxin (TA) system. A site-specific tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Does not cleave tRNA(Met), tRNA(Arg2), tRNA(His), tRNA(Leu), tRNA(Phe) tRNA(Thr1), tRNA(Tyr) or tRNA(Val). Overexpression in E.coli inhibits translation, leads to loss of cell growth and degradation of tRNA(fMet), these effects are neutralized by expression of cognate antitoxin VapB. Expression also activates translation initiation at c [...] (132 aa) |
| | lysS | Similar to E. coli lysine tRNA synthetase, constitutive; suppressor of ColE1 mutation in primer RNA (AAC75928.1); Blastp hit to AAC75928.1 (505 aa), 95% identity in aa 1 - 505; Belongs to the class-II aminoacyl-tRNA synthetase family. (505 aa) |
| | yggJ | Putative cytoplasmic protein; Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit. (259 aa) |
| | yggH | Putative S-adenosylmethionine-dependent methyltransferase; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. (239 aa) |
| | cca | tRNA nucleotidyl transferase; Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities. These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases. (413 aa) |
| | dnaG | DNA primase; RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. (581 aa) |
| | ygjO | Putative methyltransferase; Specifically methylates the guanine in position 1835 (m2G1835) of 23S rRNA. (378 aa) |
| | yraL | Putative methyltransferase; Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. (287 aa) |
| | deaD | Cysteine sulfinate desulfinase; DEAD-box RNA helicase involved in various cellular processes at low temperature, including ribosome biogenesis, mRNA degradation and translation initiation. (646 aa) |
| | pnp | Polynucleotide phosphorylase; Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction. Is a global regulator of virulence and persistency. (711 aa) |
| | truB | tRNA pseudouridine 5S synthase; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (314 aa) |
| | ftsJ | 23S rRNA methyltransferase; Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit. (208 aa) |
| | cafA | RNase G; Similar to E. coli bundles of cytoplasmic filaments (AAC76279.1); Blastp hit to AAC76279.1 (495 aa), 96% identity in aa 7 - 495. (489 aa) |
| | yhdG | Putative TIM-barrel enzyme; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines; Belongs to the Dus family. DusB subfamily. (321 aa) |
| | fmt | 10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus. (315 aa) |
| | sun | Putative rRNA methylase; Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA. (429 aa) |
| | rpoA | RNA polymerase, alpha subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (329 aa) |
| | trpS | Tryptophan tRNA synthetase; Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (334 aa) |
| | STM3516 | Putative cytoplasmic protein; Similar to E. coli orf, hypothetical protein (AAC73329.1); Blastp hit to AAC73329.1 (92 aa), 78% identity in aa 1 - 91. (91 aa) |
| | rtcA | RNA 3'-terminal phosphate cyclase (with b3419); Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. (339 aa) |
| | rtcB | Putative cytoplasmic protein; Similar to E. coli orf, hypothetical protein (AAC76446.1); Blastp hit to AAC76446.1 (408 aa), 87% identity in aa 1 - 408. (405 aa) |
| | yhhF | Putative methyltransferase; Specifically methylates the guanine in position 966 of 16S rRNA in the assembled 30S particle; Belongs to the methyltransferase superfamily. RsmD family. (198 aa) |
| | yhiQ | Putative SAM-dependent methyltransferase; Specifically methylates the guanosine in position 1516 of 16S rRNA. (252 aa) |
| | yhiR | Putative cytoplasmic protein; Specifically methylates the adenine in position 2030 of 23S rRNA. (280 aa) |
| | glyS | Similar to E. coli glycine tRNA synthetase, beta subunit (AAC76583.1); Blastp hit to AAC76583.1 (689 aa), 92% identity in aa 1 - 689. (689 aa) |
| | glyQ | Similar to E. coli glycine tRNA synthetase, alpha subunit (AAC76584.1); Blastp hit to AAC76584.1 (303 aa), 99% identity in aa 1 - 303. (303 aa) |
| | selA | Selenocysteine synthase; Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis; Belongs to the SelA family. (463 aa) |
| | yibK | Putative tRNA/rRNA methyltransferase; Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. (157 aa) |
| | rph | RNase PH; Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates. (238 aa) |
| | rpoZ | RNA polymerase, omega subunit; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits (By similarity). (91 aa) |
| | spoU | Putative tRNA/rRNA methyltransferase; Catalyzes the 2'-O methylation of guanosine at position 18 in tRNA; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (229 aa) |
| | STM3768 | Putative selenocysteine synthase (L-seryl-tRNA(Ser) selenium transferase). (369 aa) |
| | rnpA | RNase P; RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. (119 aa) |
| | gidB | Associated with glucose-inhibited division; Specifically methylates the N7 position of guanine in position 527 of 16S rRNA. (207 aa) |
| | rhlB | Putative helicase; DEAD-box RNA helicase involved in RNA degradation. Has RNA- dependent ATPase activity and unwinds double-stranded RNA. Belongs to the DEAD box helicase family. RhlB subfamily. (421 aa) |
| | yigW | Putative hydrolase of PHP superfamily; 3'-5' exonuclease that prefers single-stranded DNA and RNA. May play a role in the H(2)O(2)-induced DNA damage repair. Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family. TatD subfamily. (260 aa) |
| | yihZ | D-Tyr-tRNA(Tyr) deacylase; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality. Belongs to the DTD family. (145 aa) |
| | trmA | tRNA (uracil-5-)-methyltransferase; Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNAs. (366 aa) |
| | rpoB | RNA polymerase, beta subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1342 aa) |
| | rpoC | RNA polymerase, beta prime subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1407 aa) |
| | yjaD | Putative NTP pyrophosphohydrolases containing a Zn-finger; Probably nucleic-acid-binding; NADH pyrophosphatase. (SW:NUDC_SALTY). (257 aa) |
| | nfi | Endonuclease V; DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. (223 aa) |
| | yjbC | Putative pseudouridine synthase; Dual specificity enzyme that catalyzes the synthesis of pseudouridine from uracil-2604 in 23S ribosomal RNA and from uracil-35 in the anticodon of tRNA(Tyr); Belongs to the pseudouridine synthase RsuA family. (289 aa) |
| | yjbN | Putative TIM-barrel enzyme; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. (332 aa) |
| | yjeA | Putative pyruvate oxidase; With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF- P) on 'Lys-34'. Catalyzes the ATP-dependent activation of (R)-beta- lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of EF- P 'Lys-34' (Probable). Can also use L-alpha-lysine as a substrate, but probably with lower efficiency. Cannot aminoacylate tRNA(Lys) with lysine. (325 aa) |
| | orn | Oligoribonuclease; 3'-to-5' exoribonuclease specific for small oligoribonucleotides; Belongs to the oligoribonuclease family. (181 aa) |
| | miaA | Delta(2)-isopentenylpyrophosphate tRNA-adenosine transferase; Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A); Belongs to the IPP transferase family. (316 aa) |
| | vacB | Putative exoribonuclease; 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs. Belongs to the RNR ribonuclease family. RNase R subfamily. (812 aa) |
| | yjfH | Putative tRNA/rRNA methyltransferase; Specifically methylates the ribose of guanosine 2251 in 23S rRNA; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. RlmB subfamily. (243 aa) |
