DNA polymerase sliding clamp {ECO:0000255|HAMAP-Rule:MF_00317}; Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication. Belongs to the PCNA family.

DNA replication licensing factor MCM7; Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differential [...]

Flap endonuclease 1; Structure-specific nuclease with 5'-flap endonuclease and 5'- 3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. A [...]

DNA primase large subunit PriL {ECO:0000255|HAMAP-Rule:MF_00701}; Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.

Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA [...]

Replication factor A; Inhibits DNA polymerase activity of PolB, which can be overcome by RFC and PNCA. Stimulates 3'-to 5'-exonuclease activity of PolB at 30 degrees Celsius, but has no effect at 50 or 70 degrees Celsius. Bind ssDNA and replication forks; replication forks structures bind both Hel308 and this protein. Has no effect on helicase activity of Hel308; may help target the helicase to DNA substrates that require DNA re-modeling. Hel308, in presence and absence of DNA. Sequence=AAB85861.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Evidence=; Sequence=A [...]

DNA ligase; DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

DNA polymerase II small subunit; Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase; Belongs to the DNA polymerase delta/II small subunit family.

DNA polymerase PolB subunit 2; In addition to polymerase activity, this DNA polymerase exhibits 3'-5' exonuclease activity. diphosphate + DNA(n+1). protein A (RPA), while 3'-5' exonuclease activity is not. Polymerase inhibition can be overcome by replication factor C (RFC) and PCNA. Temperature dependence: Optimum temperature for DNA polymerase is 60 degrees Celsius, functional between 60 and 80 degrees Celsius. Exonuclease activity is higher at 70 than 50 degrees Celsius; exonuclease activity is associated with subunit 1. Probably binds replication protein A (RPA). split over 2 genes; [...]