node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
Daro_1619 | Daro_1620 | Daro_1619 | Daro_1620 | Acetolactate synthase, small subunit. | Acetolactate synthase, large subunit. | 0.999 |
Daro_1619 | Daro_3074 | Daro_1619 | Daro_3074 | Acetolactate synthase, small subunit. | Acetolactate synthase, small subunit. | 0.910 |
Daro_1619 | Daro_3075 | Daro_1619 | Daro_3075 | Acetolactate synthase, small subunit. | Acetolactate synthase, large subunit. | 0.996 |
Daro_1619 | ilvA | Daro_1619 | Daro_4123 | Acetolactate synthase, small subunit. | L-threonine ammonia-lyase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.965 |
Daro_1619 | ilvC | Daro_1619 | Daro_3073 | Acetolactate synthase, small subunit. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.986 |
Daro_1619 | ilvD | Daro_1619 | Daro_0302 | Acetolactate synthase, small subunit. | Dihydroxyacid dehydratase; Belongs to the IlvD/Edd family. | 0.672 |
Daro_1619 | leuB1 | Daro_1619 | Daro_0864 | Acetolactate synthase, small subunit. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. | 0.965 |
Daro_1619 | leuB2 | Daro_1619 | Daro_3775 | Acetolactate synthase, small subunit. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. | 0.965 |
Daro_1619 | leuC | Daro_1619 | Daro_0861 | Acetolactate synthase, small subunit. | 3-isopropylmalate dehydratase, large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. | 0.824 |
Daro_1619 | leuD | Daro_1619 | Daro_0863 | Acetolactate synthase, small subunit. | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | 0.822 |
Daro_1620 | Daro_1619 | Daro_1620 | Daro_1619 | Acetolactate synthase, large subunit. | Acetolactate synthase, small subunit. | 0.999 |
Daro_1620 | Daro_3074 | Daro_1620 | Daro_3074 | Acetolactate synthase, large subunit. | Acetolactate synthase, small subunit. | 0.999 |
Daro_1620 | Daro_3075 | Daro_1620 | Daro_3075 | Acetolactate synthase, large subunit. | Acetolactate synthase, large subunit. | 0.909 |
Daro_1620 | ilvA | Daro_1620 | Daro_4123 | Acetolactate synthase, large subunit. | L-threonine ammonia-lyase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.954 |
Daro_1620 | ilvC | Daro_1620 | Daro_3073 | Acetolactate synthase, large subunit. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.995 |
Daro_1620 | ilvD | Daro_1620 | Daro_0302 | Acetolactate synthase, large subunit. | Dihydroxyacid dehydratase; Belongs to the IlvD/Edd family. | 0.934 |
Daro_1620 | leuB1 | Daro_1620 | Daro_0864 | Acetolactate synthase, large subunit. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. | 0.978 |
Daro_1620 | leuB2 | Daro_1620 | Daro_3775 | Acetolactate synthase, large subunit. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. | 0.979 |
Daro_1620 | leuC | Daro_1620 | Daro_0861 | Acetolactate synthase, large subunit. | 3-isopropylmalate dehydratase, large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. | 0.782 |
Daro_1620 | leuD | Daro_1620 | Daro_0863 | Acetolactate synthase, large subunit. | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | 0.757 |