Phosphomethylpyrimidine synthase; Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.

Putative Transcriptional activator; Catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, a reaction that is part of a thiamine salvage pathway; Belongs to the TenA family.

Thiazole synthase; Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S.

Glutamate-1-semialdehyde 2,1-aminomutase.

Putative MutT/nudix family protein/thiamine-phosphate pyrophosphorylase; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Belongs to the Nudix hydrolase family.

Sulfur carrier protein ThiS.

tRNA sulfurtransferase; Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

FAD-dependent glycine/D-amino acid oxidase; Catalyzes the oxidation of glycine, leading to glyoxyl imine and hydrogen peroxide as primary products; glyoxyl imine is used for the biosynthesis of the thiazole ring of thiamine. Otherwise, glyoxyl imine is spontaneously hydrolyzed in water to produce glyoxylate and ammonia. Can also use sarcosine (N-methylglycine) as substrate, and, to a lesser extent, N-ethylglycine and D-proline. Has no activity towards other amino-acids D-Asp, D-Glu, D-Gln, D-His, D-Leu, D-Lys, D- ornithine, D-Trp, D-Val, L-Ala, L-Asp, L-Glu, L-His, L-Leu, L-Lys, L- Met [...]