TonB-dependent receptor SusC; Mediates transport of starch oligosaccharides from the surface of the outer membrane to the periplasm for subsequent degradation. pass membrane protein Sequence=AAA95938.1; Type=Frameshift; Positions=374, 380, 714, 724, 745; Evidence=; sp|Q8A1G1|SUSC_BACTN;evalue=5e-058; PctID=36.07; score=227.

Polygalacturonase; Seems to regulate the surface properties of the bacterium in the presence of plant cells or plant cell extracts. galactosiduronic linkages in pectate and other galacturonans. extract; sp|P27644|PGLR_RHIRD;evalue=2e-027; PctID=36.40; score=124; Belongs to the glycosyl hydrolase 28 family.

Sensor histidine kinase TmoS; Member of the two-component regulatory system TmoS/TmoT involved in the regulation of toluene degradation. Probably phosphorylates TmoT via a four-step phosphorelay in response to toluene. Can also be induced by benzene and ethylbenzene. phospho-L-histidine. antagonists. Binding of agonists such as toluene or benzene to TmoS stimulates autophosphorylation. Toluene causes the most pronounced increase, followed by benzene, chlorobenzene and ethylbenzene. Activity is inhibited by antagonists such as o-xylene, o-chlorotoluene and trimethylbenzene isomers, whic [...]

Pectinesterase 31; Acts in the modification of cell walls via demethylesterification of cell wall pectin (By similarity). Acts in a blockwise manner, resulting in a cell wall rigidification. inhibited by kiwi pectin methylesterase inhibitor (PMEI). pH dependence: Optimum pH is 8.0-8.5; Temperature dependence: Fully active at 0 or 30 degrees Celsius, inactive at 55 degrees Celsius.; D-gluconate from pectin: step 1/5. siliques. family that do not contain a transmembrane or a signal peptide; sp|Q9LVQ0|PME31_ARATH;evalue=6e-039; PctID=45.00; score=163.

Rhamnogalacturonan acetylesterase RhgT; May play a role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also active toward acetylated xylan. diethylpyrocarbonate at 5 mM and completely inhibited by phenylmethylsulfonyl fluoride (PMSF) at 50 mM. Dimethyl phosphite achieves only a 53% inhibition. Also inhibited by metal ions (magnesium, manganese and calcium) and chelating agent (EDTA) at the same level [...]

Unsaturated rhamnogalacturonyl hydrolase YteR; Catalyzes the hydrolysis of unsaturated rhamnogalacturonan disaccharide to yield unsaturated D-galacturonic acid and L-rhamnose. It cannot act on unsaturated glucuronyl hydrolase (UGL) substrates containing unsaturated D-glucuronic acid at the non-reducing terminus, although the active pockets of YesR and UGL are very similar. enopyranuronosyl)-alpha-L-rhamnopyranose + H(2)O = 5-dehydro-4-deoxy-D-glucuronate + L-rhamnopyranose. Kinetic parameters: KM=100 uM for unsaturated rhamnogalacturonan disaccharide (at pH 4 and 30 degrees Celsius); p [...]

Putative pectate lyase C; Pectinolytic enzyme consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal glycosidic bonds of highly methylated pectins. Favors pectate, the anion, over pectin, the methyl ester (By similarity). galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends. Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence=; Note=Binds 1 Ca(2+) ion per [...]