| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| ECA3661 | dusA | ECA3661 | ECA3660 | Putative membrane protein; Similar to Yersinia pestis putative exported protein ypo0318 or y0575 SWALL:AAM84163 (EMBL:AJ414142) (72 aa) fasta scores: E(): 3.3e-15, 66.66% id in 69 aa, and to Salmonella typhimurium, and Salmonella typhi putative inner membrane protein yjbo or stm4244 or sty4440 SWALL:Q8XFK0 (EMBL:AE008898) (80 aa) fasta scores: E(): 9.7e-13, 53.84% id in 78 aa. | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | 0.756 |
| dusA | ECA3661 | ECA3660 | ECA3661 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | Putative membrane protein; Similar to Yersinia pestis putative exported protein ypo0318 or y0575 SWALL:AAM84163 (EMBL:AJ414142) (72 aa) fasta scores: E(): 3.3e-15, 66.66% id in 69 aa, and to Salmonella typhimurium, and Salmonella typhi putative inner membrane protein yjbo or stm4244 or sty4440 SWALL:Q8XFK0 (EMBL:AE008898) (80 aa) fasta scores: E(): 9.7e-13, 53.84% id in 78 aa. | 0.756 |
| dusA | fis | ECA3660 | ECA0255 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | DNA-binding protein; Activates ribosomal RNA transcription. Plays a direct role in upstream activation of rRNA promoters; Belongs to the transcriptional regulatory Fis family. | 0.475 |
| dusA | gyrB | ECA3660 | ECA4438 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | DNA gyrase subunit B; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. | 0.544 |
| dusA | hrcC | ECA3660 | ECA2100 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | Type III secretion protein; Similar to Pectobacterium carotovorum subsp. carotovorum HrcC SWALL:Q93FZ1 (EMBL:AF302656) (689 aa) fasta scores: E(): 0, 95.78% id in 688 aa, and to Erwinia amylovora HrcC precursor SWALL:Q46625 (EMBL:U56662) (676 aa) fasta scores: E(): 1.3e-169, 68.65% id in 654 aa. | 0.542 |
| dusA | ispH | ECA3660 | ECA3873 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | IspH protein (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase); Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis. | 0.694 |
| dusA | trmB | ECA3660 | ECA0977 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | Putative methyltransferase; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. | 0.522 |
| dusA | trmJ | ECA3660 | ECA3239 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | Putative RNA methyltransferase; Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA. | 0.474 |
| dusA | truA | ECA3660 | ECA3058 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | tRNA pseudouridine synthase A; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. | 0.513 |
| dusA | truB | ECA3660 | ECA0714 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | tRNA pseudouridine synthase B; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. | 0.608 |
| dusA | ttcA | ECA3660 | ECA1995 | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | Conserved hypothetical protein; Catalyzes the ATP-dependent 2-thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32). The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system. | 0.502 |
| fis | dusA | ECA0255 | ECA3660 | DNA-binding protein; Activates ribosomal RNA transcription. Plays a direct role in upstream activation of rRNA promoters; Belongs to the transcriptional regulatory Fis family. | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | 0.475 |
| gyrB | dusA | ECA4438 | ECA3660 | DNA gyrase subunit B; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | 0.544 |
| gyrB | truB | ECA4438 | ECA0714 | DNA gyrase subunit B; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. | tRNA pseudouridine synthase B; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. | 0.586 |
| hrcC | dusA | ECA2100 | ECA3660 | Type III secretion protein; Similar to Pectobacterium carotovorum subsp. carotovorum HrcC SWALL:Q93FZ1 (EMBL:AF302656) (689 aa) fasta scores: E(): 0, 95.78% id in 688 aa, and to Erwinia amylovora HrcC precursor SWALL:Q46625 (EMBL:U56662) (676 aa) fasta scores: E(): 1.3e-169, 68.65% id in 654 aa. | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | 0.542 |
| ispH | dusA | ECA3873 | ECA3660 | IspH protein (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase); Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis. | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | 0.694 |
| trmB | dusA | ECA0977 | ECA3660 | Putative methyltransferase; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. | Putative dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. | 0.522 |
| trmB | trmJ | ECA0977 | ECA3239 | Putative methyltransferase; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. | Putative RNA methyltransferase; Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA. | 0.449 |
| trmB | truA | ECA0977 | ECA3058 | Putative methyltransferase; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. | tRNA pseudouridine synthase A; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. | 0.637 |
| trmB | truB | ECA0977 | ECA0714 | Putative methyltransferase; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. | tRNA pseudouridine synthase B; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. | 0.812 |
