| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| cbiM | larA | lp_0102 | lp_0104 | Cobalt ABC transporter, substrate-binding protein; Probably part of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. Belongs to the CbiM family. NikM subfamily. | Lactate racemization operon protein LarA; Catalyzes the interconversion between the D- and L-isomers of lactate. May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient. D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance. | 0.883 |
| cbiM | larB | lp_0102 | lp_0105 | Cobalt ABC transporter, substrate-binding protein; Probably part of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. Belongs to the CbiM family. NikM subfamily. | Lactate racemization operon protein LarB; Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Carboxylates the pyridinium ring of nicotinic acid adenine dinucleotide (NaAD) and cleaves the phosphoanhydride bond to release AMP and generate pyridinium-3,5- biscarboxylic acid mononucleotide (P2CMN). LarB can hydrolyze NaAD directly or it first forms an adduct with NaAD that releases AMP and reacts with bicarbonate/CO2 to generate P2CMN. Is required for the activation of the lactate racemase LarA. May also be involved in the activation of other nickel-pin [...] | 0.899 |
| cbiM | larC1 | lp_0102 | lp_0106 | Cobalt ABC transporter, substrate-binding protein; Probably part of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. Belongs to the CbiM family. NikM subfamily. | Lactate racemization operon protein LarC,N-terminal domain; Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN), to form the mature cofactor. Is required for the activation of the lactate racemase LarA. May also be involved in the activation of other nickel-pincer cofactor-dependent enzymes. | 0.880 |
| cbiM | larC2 | lp_0102 | lp_0107 | Cobalt ABC transporter, substrate-binding protein; Probably part of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. Belongs to the CbiM family. NikM subfamily. | Lactate racemization operon protein LarC,C-terminal domain. | 0.836 |
| cbiM | larE | lp_0102 | lp_0109 | Cobalt ABC transporter, substrate-binding protein; Probably part of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. Belongs to the CbiM family. NikM subfamily. | Lactate racemization operon protein LarE; Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Catalyzes the ATP-dependent incorporation of two sulfur atoms in pyridinium-3,5-biscarboxylic acid mononucleotide (P2CMN) to yield pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN). The source of sulfur is the enzyme itself: Cys-176 of LarE is the sulfur donor, thereby being converted into dehydroalanine, and is not regenerated in vivo. Thus, two molecules of LarE undergo sacrificial sulfur transfer to create one P2TMN. Binds nickel. Is required fo [...] | 0.896 |
| cbiM | larQ | lp_0102 | lp_0101 | Cobalt ABC transporter, substrate-binding protein; Probably part of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. Belongs to the CbiM family. NikM subfamily. | Cobalt ABC transporter, permease protein; Probable transmembrane component of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. | 0.999 |
| cbiM | larR | lp_0102 | lp_0103 | Cobalt ABC transporter, substrate-binding protein; Probably part of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. Belongs to the CbiM family. NikM subfamily. | Transcription regulator, Crp/FNR family; Positive transcriptional regulator that is absolutely required for the expression of lactate racemase (Lar) activity. Controls Lar expression by sensing the L-/D-lactate ration. Binds to a 16-bp palindromic sequence (Lar box motif) that is present in the larR- larA intergenic region, allowing transcription of the larABCDE operon. | 0.987 |
| glpF1 | larA | lp_0108 | lp_0104 | Glycerol uptake facilitator protein; Transporter that facilitates the transmembrane diffusion of D/L-lactic acid. Is involved in the cellular racemization of lactate and lactate metabolism. The transported molecule is indeed lactic acid and not the lactate anion, in agreement with the assumption that, with very few exceptions, MIPs (major intrinsic proteins) only facilitate the transport of uncharged solutes. Also facilitates urea and H(2)O(2) diffusion across membranes, but is not permeable to water, glycerol and dihydroxyacetone; Belongs to the MIP/aquaporin (TC 1.A.8) family. | Lactate racemization operon protein LarA; Catalyzes the interconversion between the D- and L-isomers of lactate. May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient. D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance. | 0.883 |
| glpF1 | larB | lp_0108 | lp_0105 | Glycerol uptake facilitator protein; Transporter that facilitates the transmembrane diffusion of D/L-lactic acid. Is involved in the cellular racemization of lactate and lactate metabolism. The transported molecule is indeed lactic acid and not the lactate anion, in agreement with the assumption that, with very few exceptions, MIPs (major intrinsic proteins) only facilitate the transport of uncharged solutes. Also facilitates urea and H(2)O(2) diffusion across membranes, but is not permeable to water, glycerol and dihydroxyacetone; Belongs to the MIP/aquaporin (TC 1.A.8) family. | Lactate racemization operon protein LarB; Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Carboxylates the pyridinium ring of nicotinic acid adenine dinucleotide (NaAD) and cleaves the phosphoanhydride bond to release AMP and generate pyridinium-3,5- biscarboxylic acid mononucleotide (P2CMN). LarB can hydrolyze NaAD directly or it first forms an adduct with NaAD that releases AMP and reacts with bicarbonate/CO2 to generate P2CMN. Is required for the activation of the lactate racemase LarA. May also be involved in the activation of other nickel-pin [...] | 0.920 |
| glpF1 | larC1 | lp_0108 | lp_0106 | Glycerol uptake facilitator protein; Transporter that facilitates the transmembrane diffusion of D/L-lactic acid. Is involved in the cellular racemization of lactate and lactate metabolism. The transported molecule is indeed lactic acid and not the lactate anion, in agreement with the assumption that, with very few exceptions, MIPs (major intrinsic proteins) only facilitate the transport of uncharged solutes. Also facilitates urea and H(2)O(2) diffusion across membranes, but is not permeable to water, glycerol and dihydroxyacetone; Belongs to the MIP/aquaporin (TC 1.A.8) family. | Lactate racemization operon protein LarC,N-terminal domain; Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN), to form the mature cofactor. Is required for the activation of the lactate racemase LarA. May also be involved in the activation of other nickel-pincer cofactor-dependent enzymes. | 0.928 |
| glpF1 | larC2 | lp_0108 | lp_0107 | Glycerol uptake facilitator protein; Transporter that facilitates the transmembrane diffusion of D/L-lactic acid. Is involved in the cellular racemization of lactate and lactate metabolism. The transported molecule is indeed lactic acid and not the lactate anion, in agreement with the assumption that, with very few exceptions, MIPs (major intrinsic proteins) only facilitate the transport of uncharged solutes. Also facilitates urea and H(2)O(2) diffusion across membranes, but is not permeable to water, glycerol and dihydroxyacetone; Belongs to the MIP/aquaporin (TC 1.A.8) family. | Lactate racemization operon protein LarC,C-terminal domain. | 0.814 |
| glpF1 | larE | lp_0108 | lp_0109 | Glycerol uptake facilitator protein; Transporter that facilitates the transmembrane diffusion of D/L-lactic acid. Is involved in the cellular racemization of lactate and lactate metabolism. The transported molecule is indeed lactic acid and not the lactate anion, in agreement with the assumption that, with very few exceptions, MIPs (major intrinsic proteins) only facilitate the transport of uncharged solutes. Also facilitates urea and H(2)O(2) diffusion across membranes, but is not permeable to water, glycerol and dihydroxyacetone; Belongs to the MIP/aquaporin (TC 1.A.8) family. | Lactate racemization operon protein LarE; Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Catalyzes the ATP-dependent incorporation of two sulfur atoms in pyridinium-3,5-biscarboxylic acid mononucleotide (P2CMN) to yield pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN). The source of sulfur is the enzyme itself: Cys-176 of LarE is the sulfur donor, thereby being converted into dehydroalanine, and is not regenerated in vivo. Thus, two molecules of LarE undergo sacrificial sulfur transfer to create one P2TMN. Binds nickel. Is required fo [...] | 0.933 |
| glpF1 | larQ | lp_0108 | lp_0101 | Glycerol uptake facilitator protein; Transporter that facilitates the transmembrane diffusion of D/L-lactic acid. Is involved in the cellular racemization of lactate and lactate metabolism. The transported molecule is indeed lactic acid and not the lactate anion, in agreement with the assumption that, with very few exceptions, MIPs (major intrinsic proteins) only facilitate the transport of uncharged solutes. Also facilitates urea and H(2)O(2) diffusion across membranes, but is not permeable to water, glycerol and dihydroxyacetone; Belongs to the MIP/aquaporin (TC 1.A.8) family. | Cobalt ABC transporter, permease protein; Probable transmembrane component of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. | 0.798 |
| glpF1 | larR | lp_0108 | lp_0103 | Glycerol uptake facilitator protein; Transporter that facilitates the transmembrane diffusion of D/L-lactic acid. Is involved in the cellular racemization of lactate and lactate metabolism. The transported molecule is indeed lactic acid and not the lactate anion, in agreement with the assumption that, with very few exceptions, MIPs (major intrinsic proteins) only facilitate the transport of uncharged solutes. Also facilitates urea and H(2)O(2) diffusion across membranes, but is not permeable to water, glycerol and dihydroxyacetone; Belongs to the MIP/aquaporin (TC 1.A.8) family. | Transcription regulator, Crp/FNR family; Positive transcriptional regulator that is absolutely required for the expression of lactate racemase (Lar) activity. Controls Lar expression by sensing the L-/D-lactate ration. Binds to a 16-bp palindromic sequence (Lar box motif) that is present in the larR- larA intergenic region, allowing transcription of the larABCDE operon. | 0.954 |
| larA | cbiM | lp_0104 | lp_0102 | Lactate racemization operon protein LarA; Catalyzes the interconversion between the D- and L-isomers of lactate. May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient. D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance. | Cobalt ABC transporter, substrate-binding protein; Probably part of the energy-coupling factor (ECF) transporter complex LarMNQO involved in nickel import. Belongs to the CbiM family. NikM subfamily. | 0.883 |
| larA | glpF1 | lp_0104 | lp_0108 | Lactate racemization operon protein LarA; Catalyzes the interconversion between the D- and L-isomers of lactate. May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient. D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance. | Glycerol uptake facilitator protein; Transporter that facilitates the transmembrane diffusion of D/L-lactic acid. Is involved in the cellular racemization of lactate and lactate metabolism. The transported molecule is indeed lactic acid and not the lactate anion, in agreement with the assumption that, with very few exceptions, MIPs (major intrinsic proteins) only facilitate the transport of uncharged solutes. Also facilitates urea and H(2)O(2) diffusion across membranes, but is not permeable to water, glycerol and dihydroxyacetone; Belongs to the MIP/aquaporin (TC 1.A.8) family. | 0.883 |
| larA | larB | lp_0104 | lp_0105 | Lactate racemization operon protein LarA; Catalyzes the interconversion between the D- and L-isomers of lactate. May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient. D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance. | Lactate racemization operon protein LarB; Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Carboxylates the pyridinium ring of nicotinic acid adenine dinucleotide (NaAD) and cleaves the phosphoanhydride bond to release AMP and generate pyridinium-3,5- biscarboxylic acid mononucleotide (P2CMN). LarB can hydrolyze NaAD directly or it first forms an adduct with NaAD that releases AMP and reacts with bicarbonate/CO2 to generate P2CMN. Is required for the activation of the lactate racemase LarA. May also be involved in the activation of other nickel-pin [...] | 0.996 |
| larA | larC1 | lp_0104 | lp_0106 | Lactate racemization operon protein LarA; Catalyzes the interconversion between the D- and L-isomers of lactate. May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient. D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance. | Lactate racemization operon protein LarC,N-terminal domain; Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN), to form the mature cofactor. Is required for the activation of the lactate racemase LarA. May also be involved in the activation of other nickel-pincer cofactor-dependent enzymes. | 0.994 |
| larA | larC2 | lp_0104 | lp_0107 | Lactate racemization operon protein LarA; Catalyzes the interconversion between the D- and L-isomers of lactate. May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient. D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance. | Lactate racemization operon protein LarC,C-terminal domain. | 0.939 |
| larA | larE | lp_0104 | lp_0109 | Lactate racemization operon protein LarA; Catalyzes the interconversion between the D- and L-isomers of lactate. May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient. D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance. | Lactate racemization operon protein LarE; Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Catalyzes the ATP-dependent incorporation of two sulfur atoms in pyridinium-3,5-biscarboxylic acid mononucleotide (P2CMN) to yield pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN). The source of sulfur is the enzyme itself: Cys-176 of LarE is the sulfur donor, thereby being converted into dehydroalanine, and is not regenerated in vivo. Thus, two molecules of LarE undergo sacrificial sulfur transfer to create one P2TMN. Binds nickel. Is required fo [...] | 0.995 |
