Glutamate synthase [NADPH] small chain; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism . It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia . Feedback-inhibited GlnA interacts with and regulates the activity of the transcriptional regulator TnrA During nitrogen limitation, TnrA is in its DNA- binding active state and turns on the transcription of genes required for nitrogen assimilation Under conditions of nitrogen exce [...]

Ammonium transporter, amt family; Functions as an ammonium and methylammonium transporter in the absence of glutamine . Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source . Required for binding of NrgB to the membrane . Interaction between GlnK-AmtB complex and TnrA protects TnrA from proteolytic degradation

Phosphoribosyl-amp cyclohydrolase / phosphoribosyl-atp pyrophosphohydrolase; In the C-terminal section; belongs to the PRA-PH family

3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate

Prenyltransferase that catalyzes in vivo the transfer of the heptaprenyl moiety of heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P), producing heptaprenylglyceryl phosphate (HepGP). This reaction is an ether-bond- formation step in the biosynthesis of archaea-type G1P-based membrane lipids found in Bacillales. To a much lesser extent, is also able to use geranyl diphosphate (GPP; C10) and geranylgeranyl diphosphate (GGPP; C20) as the prenyl donors, but not farnesyl pyrophosphate (FPP; C15). Cannot use glycerol-3-phosphate (G3P) or 3 [...]

Cryptic catabolic nad-specific glutamate dehydrogenase gudb; GudB seems to be intrinsically inactive, however spontaneous mutations removing a 9-bp direct repeat within the wild-type gudB sequence activated the GudB protein and allowed more-efficient utilization of amino acids of the glutamate family. This insertion presumably causes severe destabilization of the fold of the protein, leading to an inactive enzyme that is very quickly degraded. The cryptic GudB serves as a buffer that may compensate for mutations in the rocG gene and that can also be decryptified for the utilization of [...]

Branched-chain amino acid dehydrogenase; Catalyzes the reversible deamination of L-leucine to 4- methyl-2-oxopentanoate

Catabolic nad-specific glutamate dehydrogenase rocg; Devoted to catabolic function of glutamate (and other amino acids of the glutamate family) utilization as sole nitrogen source. It is not involved in anabolic function of glutamate biosynthesis since B.subtilis possesses only one route of glutamate biosynthesis from ammonia, catalyzed by glutamate synthase. RocG is unable to utilize glutamate or glutamine as sole carbon source and to synthesize glutamate, but it is involved in the utilization of arginine, and proline as carbon or nitrogen source. The catabolic RocG is essential for c [...]