node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
folEA | mnmA | BSU22780 | BSU27500 | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | 0.467 |
folEA | queA | BSU22780 | BSU27720 | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | S-adenosylmethionine tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). | 0.727 |
folEA | queC | BSU22780 | BSU13720 | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | Pre-queuosine 0 synthase; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Uses ammonia as nitrogen donor. | 0.831 |
folEA | queD | BSU22780 | BSU13730 | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | 6-carboxy-5,6,7,8-tetrahydropterin synthase; Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde. Belongs to the PTPS family. QueD subfamily. | 0.998 |
folEA | queE | BSU22780 | BSU13740 | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | 7-carboxy-7-deazaguanine synthase; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | 0.940 |
folEA | queF | BSU22780 | BSU13750 | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | NADPH-dependent 7-cyano-7-deazaguanine reductase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1), a late step in the queuosine pathway; Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily. | 0.881 |
folEA | queG | BSU22780 | BSU08910 | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | Epoxyqueuosine reductase; Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr). | 0.775 |
folEA | tgt | BSU22780 | BSU27710 | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | tRNA-guanine transglycosylase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the [...] | 0.745 |
folEA | tilS | BSU22780 | BSU00670 | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | tRNAile lysidine synthetase; Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. Belongs to the tRNA(Ile)-lysidine synthase family. | 0.414 |
mnmA | folEA | BSU27500 | BSU22780 | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | 0.467 |
mnmA | queA | BSU27500 | BSU27720 | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | S-adenosylmethionine tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). | 0.573 |
mnmA | queC | BSU27500 | BSU13720 | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | Pre-queuosine 0 synthase; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Uses ammonia as nitrogen donor. | 0.621 |
mnmA | queE | BSU27500 | BSU13740 | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | 7-carboxy-7-deazaguanine synthase; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | 0.413 |
mnmA | queF | BSU27500 | BSU13750 | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | NADPH-dependent 7-cyano-7-deazaguanine reductase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1), a late step in the queuosine pathway; Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily. | 0.499 |
mnmA | queG | BSU27500 | BSU08910 | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | Epoxyqueuosine reductase; Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr). | 0.741 |
mnmA | tgt | BSU27500 | BSU27710 | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | tRNA-guanine transglycosylase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the [...] | 0.413 |
mnmA | thiI | BSU27500 | BSU29580 | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | Putative persulfide ATP pyrophosphatase involved in thiamine biosynthesis and tRNA modification; Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. | 0.955 |
mnmA | tilS | BSU27500 | BSU00670 | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | tRNAile lysidine synthetase; Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. Belongs to the tRNA(Ile)-lysidine synthase family. | 0.872 |
queA | folEA | BSU27720 | BSU22780 | S-adenosylmethionine tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). | GTP cyclohydrolase I; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the GTP cyclohydrolase I family. | 0.727 |
queA | mnmA | BSU27720 | BSU27500 | S-adenosylmethionine tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). | tRNA 2-thiouridylase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. | 0.573 |