| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| AFE_0407 | ilvA | AFE_0407 | AFE_0722 | Aspartate kinase, monofunctional class; Identified by similarity to SP:P41403; match to protein family HMM PF00696; match to protein family HMM PF01842; match to protein family HMM TIGR00656; match to protein family HMM TIGR00657; Belongs to the aspartokinase family. | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.485 |
| AFE_0407 | metM | AFE_0407 | AFE_0473 | Aspartate kinase, monofunctional class; Identified by similarity to SP:P41403; match to protein family HMM PF00696; match to protein family HMM PF01842; match to protein family HMM TIGR00656; match to protein family HMM TIGR00657; Belongs to the aspartokinase family. | Homoserine dehydrogenase; Identified by similarity to SP:P08499; match to protein family HMM PF00742; match to protein family HMM PF01113; match to protein family HMM PF01842; match to protein family HMM PF03447. | 0.956 |
| AFE_0407 | serC | AFE_0407 | AFE_0895 | Aspartate kinase, monofunctional class; Identified by similarity to SP:P41403; match to protein family HMM PF00696; match to protein family HMM PF01842; match to protein family HMM TIGR00656; match to protein family HMM TIGR00657; Belongs to the aspartokinase family. | Phosphoserine aminotransferase; Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. | 0.647 |
| AFE_0407 | thrC | AFE_0407 | AFE_0474 | Aspartate kinase, monofunctional class; Identified by similarity to SP:P41403; match to protein family HMM PF00696; match to protein family HMM PF01842; match to protein family HMM TIGR00656; match to protein family HMM TIGR00657; Belongs to the aspartokinase family. | Threonine synthase; Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine. | 0.884 |
| AFE_0475 | metM | AFE_0475 | AFE_0473 | Conserved hypothetical protein; Identified by similarity to GB:AAQ60147.1. | Homoserine dehydrogenase; Identified by similarity to SP:P08499; match to protein family HMM PF00742; match to protein family HMM PF01113; match to protein family HMM PF01842; match to protein family HMM PF03447. | 0.656 |
| AFE_0475 | recJ | AFE_0475 | AFE_0476 | Conserved hypothetical protein; Identified by similarity to GB:AAQ60147.1. | single-stranded-DNA-specific exonuclease RecJ; Identified by similarity to SP:P21893; match to protein family HMM PF01368. | 0.799 |
| AFE_0475 | thrC | AFE_0475 | AFE_0474 | Conserved hypothetical protein; Identified by similarity to GB:AAQ60147.1. | Threonine synthase; Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine. | 0.805 |
| ilvA | AFE_0407 | AFE_0722 | AFE_0407 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Aspartate kinase, monofunctional class; Identified by similarity to SP:P41403; match to protein family HMM PF00696; match to protein family HMM PF01842; match to protein family HMM TIGR00656; match to protein family HMM TIGR00657; Belongs to the aspartokinase family. | 0.485 |
| ilvA | leuD | AFE_0722 | AFE_0626 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | 0.750 |
| ilvA | metM | AFE_0722 | AFE_0473 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Homoserine dehydrogenase; Identified by similarity to SP:P08499; match to protein family HMM PF00742; match to protein family HMM PF01113; match to protein family HMM PF01842; match to protein family HMM PF03447. | 0.482 |
| ilvA | thrC | AFE_0722 | AFE_0474 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Threonine synthase; Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine. | 0.952 |
| leuD | ilvA | AFE_0626 | AFE_0722 | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.750 |
| leuD | thrC | AFE_0626 | AFE_0474 | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | Threonine synthase; Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine. | 0.830 |
| metM | AFE_0407 | AFE_0473 | AFE_0407 | Homoserine dehydrogenase; Identified by similarity to SP:P08499; match to protein family HMM PF00742; match to protein family HMM PF01113; match to protein family HMM PF01842; match to protein family HMM PF03447. | Aspartate kinase, monofunctional class; Identified by similarity to SP:P41403; match to protein family HMM PF00696; match to protein family HMM PF01842; match to protein family HMM TIGR00656; match to protein family HMM TIGR00657; Belongs to the aspartokinase family. | 0.956 |
| metM | AFE_0475 | AFE_0473 | AFE_0475 | Homoserine dehydrogenase; Identified by similarity to SP:P08499; match to protein family HMM PF00742; match to protein family HMM PF01113; match to protein family HMM PF01842; match to protein family HMM PF03447. | Conserved hypothetical protein; Identified by similarity to GB:AAQ60147.1. | 0.656 |
| metM | ilvA | AFE_0473 | AFE_0722 | Homoserine dehydrogenase; Identified by similarity to SP:P08499; match to protein family HMM PF00742; match to protein family HMM PF01113; match to protein family HMM PF01842; match to protein family HMM PF03447. | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.482 |
| metM | recJ | AFE_0473 | AFE_0476 | Homoserine dehydrogenase; Identified by similarity to SP:P08499; match to protein family HMM PF00742; match to protein family HMM PF01113; match to protein family HMM PF01842; match to protein family HMM PF03447. | single-stranded-DNA-specific exonuclease RecJ; Identified by similarity to SP:P21893; match to protein family HMM PF01368. | 0.656 |
| metM | thrB | AFE_0473 | AFE_3097 | Homoserine dehydrogenase; Identified by similarity to SP:P08499; match to protein family HMM PF00742; match to protein family HMM PF01113; match to protein family HMM PF01842; match to protein family HMM PF03447. | Homoserine kinase; Identified by match to protein family HMM PF01636; match to protein family HMM TIGR00938; Belongs to the pseudomonas-type ThrB family. | 0.928 |
| metM | thrC | AFE_0473 | AFE_0474 | Homoserine dehydrogenase; Identified by similarity to SP:P08499; match to protein family HMM PF00742; match to protein family HMM PF01113; match to protein family HMM PF01842; match to protein family HMM PF03447. | Threonine synthase; Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine. | 0.977 |
| pdxA | serC | AFE_0165 | AFE_0895 | 4-hydroxythreonine-4-phosphate dehydrogenase; Catalyzes the NAD(P)-dependent oxidation of 4-(phosphooxy)-L- threonine (HTP) into 2-amino-3-oxo-4-(phosphooxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP). | Phosphoserine aminotransferase; Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. | 0.917 |
