| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| asd | ilvC | AFE_2074 | AFE_0747 | Aspartate-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.408 |
| asd | ilvN | AFE_2074 | AFE_0746 | Aspartate-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family. | Acetolactate synthase, small subunit; Identified by similarity to SP:P00894; match to protein family HMM PF01842; match to protein family HMM TIGR00119. | 0.457 |
| asd | leuB | AFE_2074 | AFE_2075 | Aspartate-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.899 |
| citZ | icd | AFE_3065 | AFE_0424 | Citrate synthase II; Identified by similarity to SP:P39120; match to protein family HMM PF00285; match to protein family HMM TIGR01800; Belongs to the citrate synthase family. | Isocitrate dehydrogenase, NADP-dependent; Identified by match to protein family HMM PF00180; match to protein family HMM TIGR00183. | 0.979 |
| citZ | leuB | AFE_3065 | AFE_2075 | Citrate synthase II; Identified by similarity to SP:P39120; match to protein family HMM PF00285; match to protein family HMM TIGR01800; Belongs to the citrate synthase family. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.950 |
| icd | citZ | AFE_0424 | AFE_3065 | Isocitrate dehydrogenase, NADP-dependent; Identified by match to protein family HMM PF00180; match to protein family HMM TIGR00183. | Citrate synthase II; Identified by similarity to SP:P39120; match to protein family HMM PF00285; match to protein family HMM TIGR01800; Belongs to the citrate synthase family. | 0.979 |
| icd | leuB | AFE_0424 | AFE_2075 | Isocitrate dehydrogenase, NADP-dependent; Identified by match to protein family HMM PF00180; match to protein family HMM TIGR00183. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.897 |
| ilvA | ilvB | AFE_0722 | AFE_0745 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acetolactate synthase, large subunit, biosynthetic type; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; match to protein family HMM TIGR00118. | 0.982 |
| ilvA | ilvC | AFE_0722 | AFE_0747 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.900 |
| ilvA | ilvN | AFE_0722 | AFE_0746 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acetolactate synthase, small subunit; Identified by similarity to SP:P00894; match to protein family HMM PF01842; match to protein family HMM TIGR00119. | 0.979 |
| ilvA | leuB | AFE_0722 | AFE_2075 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.959 |
| ilvA | leuC | AFE_0722 | AFE_0624 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 3-isopropylmalate dehydratase, large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. | 0.723 |
| ilvA | leuD | AFE_0722 | AFE_0626 | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | 0.750 |
| ilvB | ilvA | AFE_0745 | AFE_0722 | Acetolactate synthase, large subunit, biosynthetic type; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; match to protein family HMM TIGR00118. | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.982 |
| ilvB | ilvC | AFE_0745 | AFE_0747 | Acetolactate synthase, large subunit, biosynthetic type; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; match to protein family HMM TIGR00118. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.999 |
| ilvB | ilvN | AFE_0745 | AFE_0746 | Acetolactate synthase, large subunit, biosynthetic type; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; match to protein family HMM TIGR00118. | Acetolactate synthase, small subunit; Identified by similarity to SP:P00894; match to protein family HMM PF01842; match to protein family HMM TIGR00119. | 0.999 |
| ilvB | leuB | AFE_0745 | AFE_2075 | Acetolactate synthase, large subunit, biosynthetic type; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; match to protein family HMM TIGR00118. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.966 |
| ilvB | leuC | AFE_0745 | AFE_0624 | Acetolactate synthase, large subunit, biosynthetic type; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; match to protein family HMM TIGR00118. | 3-isopropylmalate dehydratase, large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. | 0.698 |
| ilvB | leuD | AFE_0745 | AFE_0626 | Acetolactate synthase, large subunit, biosynthetic type; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; match to protein family HMM TIGR00118. | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | 0.792 |
| ilvB | nifV | AFE_0745 | AFE_1506 | Acetolactate synthase, large subunit, biosynthetic type; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; match to protein family HMM TIGR00118. | Homocitrate synthase; Identified by similarity to SP:Q44290; match to protein family HMM PF00682; match to protein family HMM TIGR02660; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 0.596 |
