Conserved hypothetical protein; Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Pseudouridylate synthase I (truA); Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs; Belongs to the tRNA pseudouridine synthase TruA family.

Centromere/microtubule-binding protein (cbf5); Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.

SSU ribosomal protein S12P (rpsL); With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits. Belongs to the universal ribosomal protein uS12 family.

O-sialoglycoprotein endopeptidase (gcp); Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction (By similarity). The Bud32 domain probably displays kinase activity that regulates Kae1 function. In vitro, exhibits low ATPase activity, but does not bind DNA and does [...]

leucyl-tRNA synthetase (leuS); Similar to SP:P26637 PID:1370340 PID:1403549 PID:3508 percent identity: 35.17; identified by sequence similarity; putative; Belongs to the class-I aminoacyl-tRNA synthetase family.

Thermosome (ths); Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity; Belongs to the TCP-1 chaperonin family.

Conserved hypothetical protein; Similar to GB:S61233 PID:385923 GB:AE000666 percent identity: 36.10; identified by sequence similarity; putative.

Extragenic suppressor (suhB); Phosphatase with broad specificity; it can dephosphorylate fructose 1,6-bisphosphate, both D and L isomers of inositol-1-phosphate (I-1-P), 2'-AMP, pNPP, beta-glycerol phosphate, and alpha-D-glucose-1- phosphate. Cannot hydrolyze glucose-6-phosphate, fructose-6-phosphate, NAD(+) or 5'-AMP. May be involved in the biosynthesis of a unique osmolyte, di-myo-inositol 1,1-phosphate.