| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| MJ_0971 | cofF | MJ_0971 | MJ_1001 | Conserved hypothetical protein; Similar to GP:1707806 percent identity: 29.74; identified by sequence similarity; putative. | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | 0.403 |
| MJ_0971 | hisS | MJ_0971 | MJ_1000 | Conserved hypothetical protein; Similar to GP:1707806 percent identity: 29.74; identified by sequence similarity; putative. | histidyl-tRNA synthetase (hisS); Similar to SP:P30053 percent identity: 35.44; identified by sequence similarity; putative; Belongs to the class-II aminoacyl-tRNA synthetase family. | 0.585 |
| argB | argC | MJ_0069 | MJ_1096 | Acetylglutamate kinase (argB); Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate; Belongs to the acetylglutamate kinase family. ArgB subfamily. | N-acetyl-gamma-glutamyl-phosphate reductase (argC); Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. | 0.999 |
| argB | cofF | MJ_0069 | MJ_1001 | Acetylglutamate kinase (argB); Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate; Belongs to the acetylglutamate kinase family. ArgB subfamily. | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | 0.553 |
| argC | argB | MJ_1096 | MJ_0069 | N-acetyl-gamma-glutamyl-phosphate reductase (argC); Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. | Acetylglutamate kinase (argB); Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate; Belongs to the acetylglutamate kinase family. ArgB subfamily. | 0.999 |
| argC | cofF | MJ_1096 | MJ_1001 | N-acetyl-gamma-glutamyl-phosphate reductase (argC); Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | 0.492 |
| argC | rfcS | MJ_1096 | MJ_1422 | N-acetyl-gamma-glutamyl-phosphate reductase (argC); Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. | Activator 1 (replication factor C), 35 KD subunit; Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA; Belongs to the activator 1 small subunits family. RfcS subfamily. | 0.400 |
| cofE | cofF | MJ_0768 | MJ_1001 | Conserved hypothetical protein; Catalyzes the GTP-dependent successive addition of two L- glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy- 5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2), with a gamma-linkage between the two glutamates. Cannot use F420-2 as substrate to add more glutamates. Exhibits maximum activity with GTP, compared with UTP (66%) and dGTP (25%); with ATP, only F420-1 is observed as the product; CTP and TTP support no activity. Belongs to the CofE family. | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | 0.902 |
| cofF | MJ_0971 | MJ_1001 | MJ_0971 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | Conserved hypothetical protein; Similar to GP:1707806 percent identity: 29.74; identified by sequence similarity; putative. | 0.403 |
| cofF | argB | MJ_1001 | MJ_0069 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | Acetylglutamate kinase (argB); Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate; Belongs to the acetylglutamate kinase family. ArgB subfamily. | 0.553 |
| cofF | argC | MJ_1001 | MJ_1096 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | N-acetyl-gamma-glutamyl-phosphate reductase (argC); Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. | 0.492 |
| cofF | cofE | MJ_1001 | MJ_0768 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | Conserved hypothetical protein; Catalyzes the GTP-dependent successive addition of two L- glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy- 5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2), with a gamma-linkage between the two glutamates. Cannot use F420-2 as substrate to add more glutamates. Exhibits maximum activity with GTP, compared with UTP (66%) and dGTP (25%); with ATP, only F420-1 is observed as the product; CTP and TTP support no activity. Belongs to the CofE family. | 0.902 |
| cofF | dacZ | MJ_1001 | MJ_1002 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | Conserved hypothetical protein; Diadenylate cyclase that catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP is a second messenger for intracellular signal transduction involved in the control of important regulatory processes such as osmoregulation (By similarity). | 0.607 |
| cofF | hacA | MJ_1001 | MJ_1003 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | 3-isopropylmalate dehydratase (leuC); Hydro-lyase with broad substrate specificity for cis- unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4- tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4- tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1- hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)- aconitate, and even the non-physiological cis-homo(4)-aconitate with similar efficiency. These reactions are pa [...] | 0.423 |
| cofF | hisS | MJ_1001 | MJ_1000 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | histidyl-tRNA synthetase (hisS); Similar to SP:P30053 percent identity: 35.44; identified by sequence similarity; putative; Belongs to the class-II aminoacyl-tRNA synthetase family. | 0.686 |
| cofF | rfcS | MJ_1001 | MJ_1422 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | Activator 1 (replication factor C), 35 KD subunit; Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA; Belongs to the activator 1 small subunits family. RfcS subfamily. | 0.506 |
| cofF | rpl23 | MJ_1001 | MJ_0178 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | LSU ribosomal protein L23P (rplW); Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Belongs to the universal ribosomal protein uL23 family. | 0.603 |
| cofF | ths | MJ_1001 | MJ_0999 | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | Thermosome (ths); Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity; Belongs to the TCP-1 chaperonin family. | 0.497 |
| dacZ | cofF | MJ_1002 | MJ_1001 | Conserved hypothetical protein; Diadenylate cyclase that catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP is a second messenger for intracellular signal transduction involved in the control of important regulatory processes such as osmoregulation (By similarity). | Ribosomal protein S6 modification protein 2 (rimK); Catalyzes the ATP-dependent addition of one alpha-linked L- glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta- glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or [...] | 0.607 |
| dacZ | hacA | MJ_1002 | MJ_1003 | Conserved hypothetical protein; Diadenylate cyclase that catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP is a second messenger for intracellular signal transduction involved in the control of important regulatory processes such as osmoregulation (By similarity). | 3-isopropylmalate dehydratase (leuC); Hydro-lyase with broad substrate specificity for cis- unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4- tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4- tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1- hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)- aconitate, and even the non-physiological cis-homo(4)-aconitate with similar efficiency. These reactions are pa [...] | 0.855 |
