| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| CV_0785 | metXS | CV_0785 | CV_0786 | Conserved hypothetical protein; Identified by sequence similarity; putative; ORF located using Blastx/COG0500. | Probable homoserine O-acetyltransferase; Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine. | 0.998 |
| cysB2 | glyA | CV_3395 | CV_1286 | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.951 |
| cysB2 | ilvA | CV_3395 | CV_3093 | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.919 |
| cysB2 | metL | CV_3395 | CV_0996 | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | Homoserine dehydrogenase; Identified by sequence similarity; putative; ORF located using GeneMark/Blastx/COG0460. | 0.830 |
| cysB2 | metXS | CV_3395 | CV_0786 | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | Probable homoserine O-acetyltransferase; Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine. | 0.876 |
| cysB2 | metY | CV_3395 | CV_1934 | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | O-acetylhomoserine (thiol)-lyase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG2873. | 0.946 |
| cysB2 | metZ | CV_3395 | CV_2725 | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | O-succinylhomoserine sulfhydrylase; Catalyzes the formation of L-homocysteine from O-succinyl-L- homoserine (OSHS) and hydrogen sulfide. | 0.991 |
| cysB2 | tdcB | CV_3395 | CV_3250 | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | Threonine dehydratase; Identified by sequence similarity; putative; ORF located using GeneMark/Blastx/COG1171. | 0.919 |
| cysB2 | trpB | CV_3395 | CV_2762 | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | Tryptophan synthase, beta subunit; The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine. | 0.918 |
| glyA | cysB2 | CV_1286 | CV_3395 | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | 0.951 |
| glyA | ilvA | CV_1286 | CV_3093 | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.921 |
| glyA | metXS | CV_1286 | CV_0786 | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Probable homoserine O-acetyltransferase; Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine. | 0.849 |
| glyA | tdcB | CV_1286 | CV_3250 | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Threonine dehydratase; Identified by sequence similarity; putative; ORF located using GeneMark/Blastx/COG1171. | 0.921 |
| glyA | trpB | CV_1286 | CV_2762 | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Tryptophan synthase, beta subunit; The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine. | 0.928 |
| ilvA | cysB2 | CV_3093 | CV_3395 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Cystathionine beta-synthase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG0517. | 0.919 |
| ilvA | glyA | CV_3093 | CV_1286 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.921 |
| ilvA | lysC | CV_3093 | CV_1018 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Aspartate kinase; Identified by sequence similarity; putative; ORF located using GeneMark/Blastx/COG0527; Belongs to the aspartokinase family. | 0.498 |
| ilvA | metL | CV_3093 | CV_0996 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Homoserine dehydrogenase; Identified by sequence similarity; putative; ORF located using GeneMark/Blastx/COG0460. | 0.508 |
| ilvA | metXS | CV_3093 | CV_0786 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Probable homoserine O-acetyltransferase; Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine. | 0.852 |
| ilvA | metY | CV_3093 | CV_1934 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | O-acetylhomoserine (thiol)-lyase; Identified by sequence similarity; putative; ORF located using Glimmer/GeneMark/Blastx/COG2873. | 0.808 |
