| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| CUS_7361 | CUS_7787 | CUS_7361 | CUS_7787 | Prephenate dehydratase; Identified by match to protein family HMM PF00800; match to protein family HMM PF01817. | Putative methionine synthase; Identified by match to protein family HMM PF00809; match to protein family HMM PF02310; match to protein family HMM PF02574; match to protein family HMM PF02607. | 0.552 |
| CUS_7361 | glyA | CUS_7361 | CUS_6839 | Prephenate dehydratase; Identified by match to protein family HMM PF00800; match to protein family HMM PF01817. | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.520 |
| CUS_7361 | ilvA | CUS_7361 | CUS_6532 | Prephenate dehydratase; Identified by match to protein family HMM PF00800; match to protein family HMM PF01817. | Threonine ammonia-lyase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.683 |
| CUS_7361 | metE | CUS_7361 | CUS_4364 | Prephenate dehydratase; Identified by match to protein family HMM PF00800; match to protein family HMM PF01817. | Putative 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase; Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. | 0.541 |
| CUS_7361 | metF | CUS_7361 | CUS_7369 | Prephenate dehydratase; Identified by match to protein family HMM PF00800; match to protein family HMM PF01817. | Methylenetetrahydrofolate reductase (NAD(P)H); Identified by match to protein family HMM PF02219; match to protein family HMM TIGR00676; Belongs to the methylenetetrahydrofolate reductase family. | 0.849 |
| CUS_7361 | metG | CUS_7361 | CUS_6565 | Prephenate dehydratase; Identified by match to protein family HMM PF00800; match to protein family HMM PF01817. | methionine--tRNA ligase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. | 0.411 |
| CUS_7361 | metK | CUS_7361 | CUS_8045 | Prephenate dehydratase; Identified by match to protein family HMM PF00800; match to protein family HMM PF01817. | Methionine adenosyltransferase; Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. | 0.533 |
| CUS_7786 | CUS_7787 | CUS_7786 | CUS_7787 | Vitamin B12 dependent methionine synthase, activation domain protein; Identified by match to protein family HMM PF02965. | Putative methionine synthase; Identified by match to protein family HMM PF00809; match to protein family HMM PF02310; match to protein family HMM PF02574; match to protein family HMM PF02607. | 0.981 |
| CUS_7786 | glyA | CUS_7786 | CUS_6839 | Vitamin B12 dependent methionine synthase, activation domain protein; Identified by match to protein family HMM PF02965. | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.447 |
| CUS_7786 | ilvA | CUS_7786 | CUS_6532 | Vitamin B12 dependent methionine synthase, activation domain protein; Identified by match to protein family HMM PF02965. | Threonine ammonia-lyase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.862 |
| CUS_7786 | metE | CUS_7786 | CUS_4364 | Vitamin B12 dependent methionine synthase, activation domain protein; Identified by match to protein family HMM PF02965. | Putative 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase; Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. | 0.460 |
| CUS_7786 | metF | CUS_7786 | CUS_7369 | Vitamin B12 dependent methionine synthase, activation domain protein; Identified by match to protein family HMM PF02965. | Methylenetetrahydrofolate reductase (NAD(P)H); Identified by match to protein family HMM PF02219; match to protein family HMM TIGR00676; Belongs to the methylenetetrahydrofolate reductase family. | 0.939 |
| CUS_7786 | metG | CUS_7786 | CUS_6565 | Vitamin B12 dependent methionine synthase, activation domain protein; Identified by match to protein family HMM PF02965. | methionine--tRNA ligase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. | 0.782 |
| CUS_7786 | metK | CUS_7786 | CUS_8045 | Vitamin B12 dependent methionine synthase, activation domain protein; Identified by match to protein family HMM PF02965. | Methionine adenosyltransferase; Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. | 0.723 |
| CUS_7787 | CUS_7361 | CUS_7787 | CUS_7361 | Putative methionine synthase; Identified by match to protein family HMM PF00809; match to protein family HMM PF02310; match to protein family HMM PF02574; match to protein family HMM PF02607. | Prephenate dehydratase; Identified by match to protein family HMM PF00800; match to protein family HMM PF01817. | 0.552 |
| CUS_7787 | CUS_7786 | CUS_7787 | CUS_7786 | Putative methionine synthase; Identified by match to protein family HMM PF00809; match to protein family HMM PF02310; match to protein family HMM PF02574; match to protein family HMM PF02607. | Vitamin B12 dependent methionine synthase, activation domain protein; Identified by match to protein family HMM PF02965. | 0.981 |
| CUS_7787 | folD | CUS_7787 | CUS_5980 | Putative methionine synthase; Identified by match to protein family HMM PF00809; match to protein family HMM PF02310; match to protein family HMM PF02574; match to protein family HMM PF02607. | Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain protein; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 0.865 |
| CUS_7787 | glyA | CUS_7787 | CUS_6839 | Putative methionine synthase; Identified by match to protein family HMM PF00809; match to protein family HMM PF02310; match to protein family HMM PF02574; match to protein family HMM PF02607. | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.927 |
| CUS_7787 | ilvA | CUS_7787 | CUS_6532 | Putative methionine synthase; Identified by match to protein family HMM PF00809; match to protein family HMM PF02310; match to protein family HMM PF02574; match to protein family HMM PF02607. | Threonine ammonia-lyase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.947 |
| CUS_7787 | metE | CUS_7787 | CUS_4364 | Putative methionine synthase; Identified by match to protein family HMM PF00809; match to protein family HMM PF02310; match to protein family HMM PF02574; match to protein family HMM PF02607. | Putative 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase; Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. | 0.988 |
