| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| clpX | smi_1557 | smi_1559 | smi_1557 | ATP-dependent Clp protease ATP-binding subunit clpX; ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP. | Conserved hypothetical protein. | 0.863 |
| clpX | smi_1560 | smi_1559 | smi_1560 | ATP-dependent Clp protease ATP-binding subunit clpX; ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP. | Conserved hypothetical protein. | 0.802 |
| clpX | smi_1561 | smi_1559 | smi_1561 | ATP-dependent Clp protease ATP-binding subunit clpX; ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP. | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | 0.957 |
| clpX | thyA | smi_1559 | smi_1477 | ATP-dependent Clp protease ATP-binding subunit clpX; ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.462 |
| fhs | glyA | smi_1192 | smi_1123 | Formate-tetrahydrofolate ligase; Belongs to the formate--tetrahydrofolate ligase family. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.979 |
| fhs | purH | smi_1192 | smi_0072 | Formate-tetrahydrofolate ligase; Belongs to the formate--tetrahydrofolate ligase family. | Phosphoribosylaminoimidazolecarboxamide formyltransferase. | 0.975 |
| fhs | purN | smi_1192 | smi_0070 | Formate-tetrahydrofolate ligase; Belongs to the formate--tetrahydrofolate ligase family. | 5'-phosphoribosylglycinamide transformylase 1; Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate. | 0.968 |
| fhs | smi_1561 | smi_1192 | smi_1561 | Formate-tetrahydrofolate ligase; Belongs to the formate--tetrahydrofolate ligase family. | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | 0.919 |
| fhs | smi_1917 | smi_1192 | smi_1917 | Formate-tetrahydrofolate ligase; Belongs to the formate--tetrahydrofolate ligase family. | folC family protein; COG0285, FolC, folylpolyglutamate synthase, bifunctional protein; Belongs to the folylpolyglutamate synthase family. | 0.572 |
| glyA | fhs | smi_1123 | smi_1192 | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Formate-tetrahydrofolate ligase; Belongs to the formate--tetrahydrofolate ligase family. | 0.979 |
| glyA | purH | smi_1123 | smi_0072 | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Phosphoribosylaminoimidazolecarboxamide formyltransferase. | 0.986 |
| glyA | purN | smi_1123 | smi_0070 | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 5'-phosphoribosylglycinamide transformylase 1; Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate. | 0.973 |
| glyA | smi_1561 | smi_1123 | smi_1561 | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | 0.937 |
| glyA | smi_1917 | smi_1123 | smi_1917 | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | folC family protein; COG0285, FolC, folylpolyglutamate synthase, bifunctional protein; Belongs to the folylpolyglutamate synthase family. | 0.589 |
| glyA | thyA | smi_1123 | smi_1477 | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.956 |
| purH | fhs | smi_0072 | smi_1192 | Phosphoribosylaminoimidazolecarboxamide formyltransferase. | Formate-tetrahydrofolate ligase; Belongs to the formate--tetrahydrofolate ligase family. | 0.975 |
| purH | glyA | smi_0072 | smi_1123 | Phosphoribosylaminoimidazolecarboxamide formyltransferase. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.986 |
| purH | purN | smi_0072 | smi_0070 | Phosphoribosylaminoimidazolecarboxamide formyltransferase. | 5'-phosphoribosylglycinamide transformylase 1; Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate. | 0.999 |
| purH | smi_1561 | smi_0072 | smi_1561 | Phosphoribosylaminoimidazolecarboxamide formyltransferase. | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | 0.910 |
| purN | fhs | smi_0070 | smi_1192 | 5'-phosphoribosylglycinamide transformylase 1; Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate. | Formate-tetrahydrofolate ligase; Belongs to the formate--tetrahydrofolate ligase family. | 0.968 |
