Protein CHROMATIN REMODELING 35; Subunit of the chromatin-remodeling complex (DDR complex) that mediates RNA polymerases IV and V (Pol IV and Pol V) recruitment to chromatin. Cooperates with Pol IV and Pol V to regulates RNA- and RNAi- (RNA interference) directed non- CpG de novo DNA methylation on cytosine of genes targeted for silencing and enhancers, also known as siRNA-directed DNA methylation (RdDM), thus leading to epigenetic modification of the genome and promoting/maintaining heterochromatin. In collaboration with Pol V, mediates/maintains, in cis, methylation- associated self- [...]

Protein argonaute 4; Together with RDM3, required for transcriptional gene silencing (TGS) by DNA methylation and repressive histone modifications (H3K9me2) of several chromatin loci. Component of the RISC complex that associate with the small interfering RNA (siRNA) pathway involved in direct cytosine methylation at endogenous DNA repeats. Forms a AGO4/NRPE1/siRNA complex in cajal body, facilitating its function in RNA-directed gene silencing of target loci. Required for CpNpG and asymmetric DNA methylation as well as histone H3 'Lys-9' methylation (H3K9me) at SUP and SN1 loci. May be [...]

DNA-directed RNA polymerase V subunit 1; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase V involved in RNA- directed DNA methylation-dependent (RdDM) silencing of endogenous repeated sequences, including transposable elements. Also required for full erasure of methylation when the RNA trigger is withdrawn. Seems also involved in the synthesis of short-interfering RNAs (siRNA). Essential component of a self-reinforcing loop coupling de novo DNA methylatio [...]

DNA (cytosine-5)-methyltransferase DRM2; Involved in de novo DNA methylation. Controls asymmetric and CpNpG methylation. Required for FWA gene silencing but not for the maintenance of SUP gene silencing. Functionally redundant to CMT3 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation (RdDM). Acts as major DNA methyltransferase in the RdDM pathway, and is essential for RNA-directed de novo DNA methylation of cytosines in all sequence contexts. Associates with long non- coding RNA (lncRNA) produced by RNA polymerase V (Pol V). This association is dependent on AGO4 [...]

DNA-directed RNA polymerases IV and V subunit 4; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation- dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements. Required for the de novo DNA methylation directed by the RdDM pathway.

RNA-directed DNA methylation 4; Probable regulatory factor for several RNA polymerases. Effector involved in facilitation of Pol V transcription as RNA scaffold and recruitment of silencing complex to target genomic sites.

DNA-directed RNA polymerases IV and V subunit 2; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. A [...]

Protein RNA-directed DNA methylation 3; Effector of RNA-directed DNA methylation (RdDM) triggered by small interfering RNAs (siRNAs, 24-nt RNAs). Functions as an adapter protein that binds scaffold transcripts generated by polymerase V and recruits AGO4 and AGO4-bound siRNAs to form an RdDM effector complex. Promotes the expression of 24-nt RNAs. Required for the initial establishment of DNA methylation. Together with AGO4, required for transcriptional gene silencing (TGS) by DNA methylation and repressive histone modifications (H3K9me2) of several chromatin loci.

Histone-lysine N-methyltransferase family member SUVH9; Histone methyltransferase family member that plays a role in gene silencing. Together with MORC6 and SUVH2, regulates the silencing of some transposable elements (TEs). According to the protein does not bind S-adenosyl-L-methionine and lacks methyltransferase activity. Instead, it may function downstream of DRM2 in RNA-directed DNA methylation, binding to methylated DNA and recruiting DNA-directed RNA polymerase V to chromatin ; Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase [...]