DNA-directed DNA polymerase; Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP- independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of repl [...]

Nuclease SbcCD subunit; SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity; Belongs to the SbcD family.

Protein RecA; Can catalyze the hydrolysis of ATP in the presence of single- stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage; Belongs to the RecA family.

Nuclease SbcCD subunit; SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity (By similarity); Belongs to the SMC family. SbcC subfamily.

Probable helicase involved in DNA repair and perhaps also replication; Belongs to the helicase family. DinG subfamily; Contains 1 helicase ATP-binding domain; KEGG: ncy:NOCYR_1167 ATP-dependent DNA helicase DinG.

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair (By similarity). Has weak intrinsic nick joining activities and accumulates DNA-adenylate. Acts as a backup for LigD in the Ku- LigD-dependent NHEJ pathway; Belongs to the ATP-dependent DNA ligase family; overlaps another CDS with the same product name.

Exodeoxyribonuclease III; Major apurinic-apyrimidinic endonuclease of E. coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities. Belongs to the DNA repair enzymes AP/ExoA family; KEGG: xfa:XF1933 exodeoxyribonuclease III.

Exodeoxyribonuclease III; Belongs to the DNA repair enzymes AP/ExoA family; KEGG: bbag:E1O_25710 exodeoxyribonuclease III.

DNA ligase (ATP); Ligates dsDNA, repairs incompatible DSB which require 3'-resection, gap filling and ligation. Has several activitie; DNA-directed DNA or RNA polymerase on 5'-overhangs, terminal transferase (extending ssDNA or blunt dsDNA in a non- templated fashion, preferentially with rNTPs), DNA-dependent RNA primase (synthesizes short RNAs on unprimed closed ssDNA) and 3'- phosphoesterase on ssDNA (By similarity); The preference of the polymerase domain for rNTPs over dNTPs may be advantageous in dormant cells, where the dNTP pool may be limiting; In the N-terminal sectio; belongs [...]