| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| MPLA_1230005 | ilvC | MPLA_1230005 | MPLA_240007 | Uncharacterized AIPM/Hcit synthase family transferase aq_356; Belongs to the alpha-IPM synthase/homocitrate synthase family. | Ketol-acid reductoisomerase (NADP(+)); Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.864 |
| MPLA_1230005 | ilvH | MPLA_1230005 | MPLA_250014 | Uncharacterized AIPM/Hcit synthase family transferase aq_356; Belongs to the alpha-IPM synthase/homocitrate synthase family. | Acetolactate synthase small subunit (AHAS) (Acetohydroxy-acid synthase small subunit) (ALS). | 0.981 |
| MPLA_1230005 | ilvI | MPLA_1230005 | MPLA_250015 | Uncharacterized AIPM/Hcit synthase family transferase aq_356; Belongs to the alpha-IPM synthase/homocitrate synthase family. | Acetolactate synthase. | 0.944 |
| MPLA_1230005 | leuB | MPLA_1230005 | MPLA_440002 | Uncharacterized AIPM/Hcit synthase family transferase aq_356; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.853 |
| MPLA_1230005 | maeB | MPLA_1230005 | MPLA_50008 | Uncharacterized AIPM/Hcit synthase family transferase aq_356; Belongs to the alpha-IPM synthase/homocitrate synthase family. | Putative fused malic enzyme oxidoreductase phosphotransacetylase. | 0.901 |
| MPLA_1230005 | maeB-2 | MPLA_1230005 | MPLA_630023 | Uncharacterized AIPM/Hcit synthase family transferase aq_356; Belongs to the alpha-IPM synthase/homocitrate synthase family. | Putative fused malic enzyme oxidoreductase phosphotransacetylase. | 0.901 |
| MPLA_1230005 | yeaU-3 | MPLA_1230005 | MPLA_1670038 | Uncharacterized AIPM/Hcit synthase family transferase aq_356; Belongs to the alpha-IPM synthase/homocitrate synthase family. | Putative tartrate dehydrogenase. | 0.756 |
| SRY | ilvA | MPLA_1070002 | MPLA_30038 | Threo-3-hydroxyaspartate ammonia-lyase. | L-threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.784 |
| SRY | ilvC | MPLA_1070002 | MPLA_240007 | Threo-3-hydroxyaspartate ammonia-lyase. | Ketol-acid reductoisomerase (NADP(+)); Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.628 |
| SRY | ilvH | MPLA_1070002 | MPLA_250014 | Threo-3-hydroxyaspartate ammonia-lyase. | Acetolactate synthase small subunit (AHAS) (Acetohydroxy-acid synthase small subunit) (ALS). | 0.965 |
| SRY | ilvI | MPLA_1070002 | MPLA_250015 | Threo-3-hydroxyaspartate ammonia-lyase. | Acetolactate synthase. | 0.940 |
| SRY | leuB | MPLA_1070002 | MPLA_440002 | Threo-3-hydroxyaspartate ammonia-lyase. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.915 |
| ilvA | SRY | MPLA_30038 | MPLA_1070002 | L-threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Threo-3-hydroxyaspartate ammonia-lyase. | 0.784 |
| ilvA | ilvC | MPLA_30038 | MPLA_240007 | L-threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Ketol-acid reductoisomerase (NADP(+)); Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.670 |
| ilvA | ilvH | MPLA_30038 | MPLA_250014 | L-threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acetolactate synthase small subunit (AHAS) (Acetohydroxy-acid synthase small subunit) (ALS). | 0.979 |
| ilvA | ilvI | MPLA_30038 | MPLA_250015 | L-threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acetolactate synthase. | 0.969 |
| ilvA | leuB | MPLA_30038 | MPLA_440002 | L-threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.917 |
| ilvA | yeaU-3 | MPLA_30038 | MPLA_1670038 | L-threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Putative tartrate dehydrogenase. | 0.527 |
| ilvC | MPLA_1230005 | MPLA_240007 | MPLA_1230005 | Ketol-acid reductoisomerase (NADP(+)); Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | Uncharacterized AIPM/Hcit synthase family transferase aq_356; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 0.864 |
| ilvC | SRY | MPLA_240007 | MPLA_1070002 | Ketol-acid reductoisomerase (NADP(+)); Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | Threo-3-hydroxyaspartate ammonia-lyase. | 0.628 |
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