node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
DJ90_2615 | DJ90_4261 | DJ90_2615 | DJ90_4261 | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 0.863 |
DJ90_2615 | ilvA | DJ90_2615 | DJ90_310 | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.537 |
DJ90_2615 | ilvB-2 | DJ90_2615 | DJ90_3365 | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | Acolac_lg: acetolactate synthase, large subunit, biosynthetic type. | 0.927 |
DJ90_2615 | ilvN | DJ90_2615 | DJ90_3364 | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | Acolac_sm: acetolactate synthase, small subunit. | 0.929 |
DJ90_2615 | leuA-2 | DJ90_2615 | DJ90_6421 | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily. | 0.863 |
DJ90_2615 | leuB | DJ90_2615 | DJ90_3362 | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.466 |
DJ90_2615 | leuC | DJ90_2615 | DJ90_2134 | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | 3-isopropylmalate dehydratase, large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. | 0.861 |
DJ90_2615 | leuD | DJ90_2615 | DJ90_2133 | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | 0.861 |
DJ90_4261 | DJ90_2615 | DJ90_4261 | DJ90_2615 | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | 0.863 |
DJ90_4261 | ilvB-2 | DJ90_4261 | DJ90_3365 | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | Acolac_lg: acetolactate synthase, large subunit, biosynthetic type. | 0.915 |
DJ90_4261 | ilvC | DJ90_4261 | DJ90_3363 | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.723 |
DJ90_4261 | ilvD | DJ90_4261 | DJ90_2433 | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | ilvD: dihydroxy-acid dehydratase; Belongs to the IlvD/Edd family. | 0.966 |
DJ90_4261 | ilvN | DJ90_4261 | DJ90_3364 | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | Acolac_sm: acetolactate synthase, small subunit. | 0.970 |
DJ90_4261 | leuA-2 | DJ90_4261 | DJ90_6421 | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily. | 0.882 |
DJ90_4261 | leuB | DJ90_4261 | DJ90_3362 | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. | 0.858 |
DJ90_4261 | leuC | DJ90_4261 | DJ90_2134 | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 3-isopropylmalate dehydratase, large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. | 0.964 |
DJ90_4261 | leuD | DJ90_4261 | DJ90_2133 | HMGL-like family protein; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | 0.958 |
ilvA | DJ90_2615 | DJ90_310 | DJ90_2615 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein; porB_KorB: 2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family protein. | 0.537 |
ilvA | ilvB-2 | DJ90_310 | DJ90_3365 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acolac_lg: acetolactate synthase, large subunit, biosynthetic type. | 0.976 |
ilvA | ilvC | DJ90_310 | DJ90_3363 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.777 |