| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| AMB84115.1 | queF | AWM79_01860 | AWM79_22240 | Cd(II)/Pb(II)-responsive transcriptional regulator; Derived by automated computational analysis using gene prediction method: Protein Homology. | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.753 |
| AMB84325.1 | queF | AWM79_03020 | AWM79_22240 | Helix-turn-helix-type transcriptional regulator; Derived by automated computational analysis using gene prediction method: Protein Homology. | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.716 |
| AMB84401.1 | queF | AWM79_03410 | AWM79_22240 | Hypothetical protein; Involved in the import of queuosine (Q) precursors, required for Q precursor salvage; Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. Q precursor transporter subfamily. | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.875 |
| AMB86112.1 | queF | AWM79_12720 | AWM79_22240 | MerR family transcriptional regulator; Derived by automated computational analysis using gene prediction method: Protein Homology. | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.752 |
| AMB87289.1 | queF | AWM79_19120 | AWM79_22240 | Cu(I)-responsive transcriptional regulator; Derived by automated computational analysis using gene prediction method: Protein Homology. | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.752 |
| AMB87848.1 | queF | AWM79_22245 | AWM79_22240 | Copper-binding protein; Derived by automated computational analysis using gene prediction method: Protein Homology. | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.803 |
| AWM79_14170 | queC | AWM79_14170 | AWM79_12070 | Type IV secretion protein Rhs; Incomplete; too short partial abutting assembly gap; missing start and stop; Derived by automated computational analysis using gene prediction method: GeneMarkS+. | 7-cyano-7-deazaguanine synthase; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | 0.957 |
| AWM79_14170 | queE | AWM79_14170 | AWM79_12065 | Type IV secretion protein Rhs; Incomplete; too short partial abutting assembly gap; missing start and stop; Derived by automated computational analysis using gene prediction method: GeneMarkS+. | 7-carboxy-7-deazaguanine synthase; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | 0.990 |
| AWM79_14170 | queF | AWM79_14170 | AWM79_22240 | Type IV secretion protein Rhs; Incomplete; too short partial abutting assembly gap; missing start and stop; Derived by automated computational analysis using gene prediction method: GeneMarkS+. | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.902 |
| AWM79_14170 | tgt | AWM79_14170 | AWM79_17105 | Type IV secretion protein Rhs; Incomplete; too short partial abutting assembly gap; missing start and stop; Derived by automated computational analysis using gene prediction method: GeneMarkS+. | tRNA guanosine(34) transglycosylase Tgt; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, - Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose t [...] | 0.411 |
| queC | AWM79_14170 | AWM79_12070 | AWM79_14170 | 7-cyano-7-deazaguanine synthase; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | Type IV secretion protein Rhs; Incomplete; too short partial abutting assembly gap; missing start and stop; Derived by automated computational analysis using gene prediction method: GeneMarkS+. | 0.957 |
| queC | queE | AWM79_12070 | AWM79_12065 | 7-cyano-7-deazaguanine synthase; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | 7-carboxy-7-deazaguanine synthase; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | 0.998 |
| queC | queF | AWM79_12070 | AWM79_22240 | 7-cyano-7-deazaguanine synthase; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.980 |
| queC | tgt | AWM79_12070 | AWM79_17105 | 7-cyano-7-deazaguanine synthase; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | tRNA guanosine(34) transglycosylase Tgt; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, - Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose t [...] | 0.507 |
| queE | AWM79_14170 | AWM79_12065 | AWM79_14170 | 7-carboxy-7-deazaguanine synthase; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | Type IV secretion protein Rhs; Incomplete; too short partial abutting assembly gap; missing start and stop; Derived by automated computational analysis using gene prediction method: GeneMarkS+. | 0.990 |
| queE | queC | AWM79_12065 | AWM79_12070 | 7-carboxy-7-deazaguanine synthase; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | 7-cyano-7-deazaguanine synthase; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | 0.998 |
| queE | queF | AWM79_12065 | AWM79_22240 | 7-carboxy-7-deazaguanine synthase; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.932 |
| queE | tgt | AWM79_12065 | AWM79_17105 | 7-carboxy-7-deazaguanine synthase; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | tRNA guanosine(34) transglycosylase Tgt; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, - Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose t [...] | 0.437 |
| queF | AMB84115.1 | AWM79_22240 | AWM79_01860 | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | Cd(II)/Pb(II)-responsive transcriptional regulator; Derived by automated computational analysis using gene prediction method: Protein Homology. | 0.753 |
| queF | AMB84325.1 | AWM79_22240 | AWM79_03020 | preQ(1) synthase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | Helix-turn-helix-type transcriptional regulator; Derived by automated computational analysis using gene prediction method: Protein Homology. | 0.716 |