Zinc finger protein lin-13; Involved in repression of vulval fate, possibly by a tumor suppressor protein Rb-mediated mechanism.

Heterochromatin protein 1 homolog.

Chromo domain-containing protein cec-4; Chromatin anchor protein which binds to methylated lysine residues on histone H3, thereby recruiting heterochromatin to the nuclear periphery. May be required for the correct positioning of chromatin and nucleoli in embryos.

Transcription factor dpl-1; Synthetic multivulva class B (synMuvB) protein. SynMuvB proteins are required to repress the induction of vulval development by Ras signaling and probably act by forming the multiprotein DRM complex that represses transcription. May also negatively regulate vulval development in association with other SynMuv class B proteins such as lin-15A. Can stimulate E2F-dependent transcription. Plays a role in negatively regulating the progression through the G1 phase of the cell cycle during postembryonic development, most likely by acting as a transcriptional repress [...]

Retinoblastoma-like protein homolog lin-35; Key regulator of cell division which acts as a transcriptional repressor and negatively regulates cell cycle progression in its active unphosphorylated form, but allows cell cycle progression when phosphorylated. When unphosphorylated and in its active form, interacts with E2F transcription factors such as efl-1 to repress their transcriptional activity and negatively regulate the progression through the G1 phase of the cell cycle during postembryonic development. May furthermore act with cell cycle regulator cki-1 to negatively regulate cell [...]

Histone-lysine N-methyltransferase set-25; Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using mono- and dimethylated H3 'Lys-9' as substrate. Acts redundantly with the methyltransferase met-2 to position chromosome arms at the nuclear lamina. Required for small-RNA-induced H3K9 methylation. Together with met-2, protects and stabilizes repeat-rich genomic regions by suppressing transcription- induced replication stress through methylation of H3K9.

Chromo domain-containing protein cec-3; Specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me), with highest preference for trimethylated 'Lys-9' (H3K9me3) followed by dimethylated 'Lys-9' (H3K9me2) followed by monomethylated 'Lys-9' (H3K9me1). Plays a role in maintaining correct unc-4 expression in the VC motor neurons where unc- 4 is expressed in the vulval but not in the non-vulval VC neurons.

Protein let-418; Part of a NuRD (Nucleosome Remodeling and Deacetylase) complex which is implicated in the synMuv B pathway that negatively regulates specification of vulval cell fate. This negative regulation is thought to be mediated via interaction with the promoter of lin-39, a key regulator in vulva development, and is dependent on the presence lin-1. Contributes to negative regulation of lag-2 which is expressed in the gut during larval development. Has a broad role in development. In association with akir-1, plays a role in regulating the transcription of antimicrobial peptide g [...]

E2F_TDP domain-containing protein; Belongs to the E2F/DP family.