| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| alsS | azo1970 | azo2474 | azo1970 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | Conserved hypothetical protein. Homology to an orf of Rhizobium sp. NGR234 of 50% (tremblnew|AAQ87234(SRS)). No domain predicted. No signal peptide or TMH reported present. | 0.751 |
| alsS | ilvA | azo2474 | azo0500 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | Probable threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.825 |
| alsS | ilvC | azo2474 | azo3156 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | IlvC protein; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.905 |
| alsS | ilvH | azo2474 | azo3155 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | Acetolactate synthase small subunit (AHAS) (Acetohydroxy-acid synthase small subunit) (ALS). InterPro: Acetolactate synthase small subunit TIGRFAM: acolac_sm: acetolactate synthase small subunit; High confidence in function and specificity. | 0.956 |
| alsS | ilvN | azo2474 | azo1035 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | Acetolactate synthase isozyme I small subunit (EC 2.2.1.6) (AHAS-I) (Acetohydroxy-acid synthase I small subunit) (ALS-I). InterPro: Acetolactate synthase small subunit acolac_sm: acetolactate synthase small subunit; High confidence in function and specificity. | 0.947 |
| alsS | leuD | azo2474 | azo1040 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | LeuD protein; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | 0.708 |
| alsS | maeB1 | azo2474 | azo0821 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | Probable malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) (NADP-ME). Homology to dme of S. meliloti of 60% (sprot|MAO1_RHIME). Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria. Tigrfam: pta: phosphotransacetylase Pfam: Malic enzyme; Phosphate acetyl/butaryltransferase no signal peptide no TMHs; High confidence in function and specificity. | 0.751 |
| alsS | maeB2 | azo2474 | azo3211 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | Probable malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+). Homology to dme of S. meliloti of 61% (sprot|MAO1_RHIME). Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria. InterPro: Phosphate acetyl/butaryl transferase (IPR002505); Malic enzyme (IPR001891) Pfam: Malic enzyme; Phosphate acetyl/butaryl transferase Tigrfam: pta: phosphotransacetylase no signal peptide no TMHs; High confidence in function and specificity. | 0.751 |
| alsS | pepM | azo2474 | azo2698 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | Phosphoenolpyruvate phosphomutase precursor(Phosphoenolpyruvate mutase) (PEP mutase) (PEP phosphomutase).FORMATION OF A CARBON-PHOSPHORUS BOND BY CONVERTING PHOSPHOENOLPYRUVATE (PEP) TO PHOSPHONOPYRUVATE (P-PYR). 34% Isocit_lyase_ph; High confidence in function and specificity. | 0.840 |
| alsS | yeaU | azo2474 | azo3860 | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | Tartrate dehydrogenase catalyzes the reduction of tartrate to oxaloglycolate. Similar to sprot|TTUC_ECOLI (75%) and to sprot|TTUC_PSEPU (76%). Pfam (PF00180): Isocitrate and isopropylmalate dehydrogenases; High confidence in function and specificity. | 0.703 |
| azo1970 | alsS | azo1970 | azo2474 | Conserved hypothetical protein. Homology to an orf of Rhizobium sp. NGR234 of 50% (tremblnew|AAQ87234(SRS)). No domain predicted. No signal peptide or TMH reported present. | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | 0.751 |
| ilvA | alsS | azo0500 | azo2474 | Probable threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | 0.825 |
| ilvA | ilvC | azo0500 | azo3156 | Probable threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | IlvC protein; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.791 |
| ilvA | ilvH | azo0500 | azo3155 | Probable threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acetolactate synthase small subunit (AHAS) (Acetohydroxy-acid synthase small subunit) (ALS). InterPro: Acetolactate synthase small subunit TIGRFAM: acolac_sm: acetolactate synthase small subunit; High confidence in function and specificity. | 0.974 |
| ilvA | ilvN | azo0500 | azo1035 | Probable threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acetolactate synthase isozyme I small subunit (EC 2.2.1.6) (AHAS-I) (Acetohydroxy-acid synthase I small subunit) (ALS-I). InterPro: Acetolactate synthase small subunit acolac_sm: acetolactate synthase small subunit; High confidence in function and specificity. | 0.929 |
| ilvA | leuD | azo0500 | azo1040 | Probable threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | LeuD protein; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. | 0.646 |
| ilvA | maeB1 | azo0500 | azo0821 | Probable threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Probable malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) (NADP-ME). Homology to dme of S. meliloti of 60% (sprot|MAO1_RHIME). Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria. Tigrfam: pta: phosphotransacetylase Pfam: Malic enzyme; Phosphate acetyl/butaryltransferase no signal peptide no TMHs; High confidence in function and specificity. | 0.437 |
| ilvA | maeB2 | azo0500 | azo3211 | Probable threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Probable malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+). Homology to dme of S. meliloti of 61% (sprot|MAO1_RHIME). Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria. InterPro: Phosphate acetyl/butaryl transferase (IPR002505); Malic enzyme (IPR001891) Pfam: Malic enzyme; Phosphate acetyl/butaryl transferase Tigrfam: pta: phosphotransacetylase no signal peptide no TMHs; High confidence in function and specificity. | 0.437 |
| ilvA | yeaU | azo0500 | azo3860 | Probable threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Tartrate dehydrogenase catalyzes the reduction of tartrate to oxaloglycolate. Similar to sprot|TTUC_ECOLI (75%) and to sprot|TTUC_PSEPU (76%). Pfam (PF00180): Isocitrate and isopropylmalate dehydrogenases; High confidence in function and specificity. | 0.661 |
| ilvC | alsS | azo3156 | azo2474 | IlvC protein; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid synthase large subunit) (ALS). InterPro: Thiamine pyrophosphate dependent enzyme TIGRFAM: acolac_lg: acetolactate synthase large subunit; Family membership; Belongs to the TPP enzyme family. | 0.905 |
