node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
azo0107 | gch | azo0107 | azo3509 | Conserved hypothetical membrane protein; Involved in the import of queuosine (Q) precursors, required for Q precursor salvage; Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. Q precursor transporter subfamily. | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.857 |
azo3118 | exsB | azo3118 | azo0413 | Lyase, putative 6-pyruvoyl tetrahydrobiopterin synthase protein InterPro: 6-pyruvoyl tetrahydropterin synthase TIGRFAM: 6PTHBS: 6-pyruvoyl tetrahydrobiopterin synthase; Specificity unclear. | Conserved hypothetical ExsB protein; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | 0.952 |
azo3118 | gch | azo3118 | azo3509 | Lyase, putative 6-pyruvoyl tetrahydrobiopterin synthase protein InterPro: 6-pyruvoyl tetrahydropterin synthase TIGRFAM: 6PTHBS: 6-pyruvoyl tetrahydrobiopterin synthase; Specificity unclear. | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.954 |
azo3118 | guaA | azo3118 | azo1581 | Lyase, putative 6-pyruvoyl tetrahydrobiopterin synthase protein InterPro: 6-pyruvoyl tetrahydropterin synthase TIGRFAM: 6PTHBS: 6-pyruvoyl tetrahydrobiopterin synthase; Specificity unclear. | GMP synthase (glutamine-hydrolyzing); Catalyzes the synthesis of GMP from XMP. | 0.496 |
azo3118 | queA | azo3118 | azo0908 | Lyase, putative 6-pyruvoyl tetrahydrobiopterin synthase protein InterPro: 6-pyruvoyl tetrahydropterin synthase TIGRFAM: 6PTHBS: 6-pyruvoyl tetrahydrobiopterin synthase; Specificity unclear. | S-adenosylmethionine:tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). | 0.490 |
azo3118 | queE | azo3118 | azo0414 | Lyase, putative 6-pyruvoyl tetrahydrobiopterin synthase protein InterPro: 6-pyruvoyl tetrahydropterin synthase TIGRFAM: 6PTHBS: 6-pyruvoyl tetrahydrobiopterin synthase; Specificity unclear. | Conserved hypothetical radical activating enzyme; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | 0.996 |
azo3118 | tgt | azo3118 | azo0907 | Lyase, putative 6-pyruvoyl tetrahydrobiopterin synthase protein InterPro: 6-pyruvoyl tetrahydropterin synthase TIGRFAM: 6PTHBS: 6-pyruvoyl tetrahydrobiopterin synthase; Specificity unclear. | Queuine tRNA-ribosyltransferase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form th [...] | 0.449 |
azo3510 | gch | azo3510 | azo3509 | InterPro: Sulfatase Membrane protein; Specificity unclear. | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.675 |
exsB | azo3118 | azo0413 | azo3118 | Conserved hypothetical ExsB protein; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | Lyase, putative 6-pyruvoyl tetrahydrobiopterin synthase protein InterPro: 6-pyruvoyl tetrahydropterin synthase TIGRFAM: 6PTHBS: 6-pyruvoyl tetrahydrobiopterin synthase; Specificity unclear. | 0.952 |
exsB | gch | azo0413 | azo3509 | Conserved hypothetical ExsB protein; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | 0.992 |
exsB | queA | azo0413 | azo0908 | Conserved hypothetical ExsB protein; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | S-adenosylmethionine:tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). | 0.527 |
exsB | queE | azo0413 | azo0414 | Conserved hypothetical ExsB protein; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | Conserved hypothetical radical activating enzyme; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. | 0.999 |
exsB | ribAB | azo0413 | azo0319 | Conserved hypothetical ExsB protein; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | GTP cyclohydrolase II; Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate; Belongs to the DHBP synthase family. | 0.441 |
exsB | tgt | azo0413 | azo0907 | Conserved hypothetical ExsB protein; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | Queuine tRNA-ribosyltransferase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form th [...] | 0.442 |
gch | azo0107 | azo3509 | azo0107 | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | Conserved hypothetical membrane protein; Involved in the import of queuosine (Q) precursors, required for Q precursor salvage; Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. Q precursor transporter subfamily. | 0.857 |
gch | azo3118 | azo3509 | azo3118 | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | Lyase, putative 6-pyruvoyl tetrahydrobiopterin synthase protein InterPro: 6-pyruvoyl tetrahydropterin synthase TIGRFAM: 6PTHBS: 6-pyruvoyl tetrahydrobiopterin synthase; Specificity unclear. | 0.954 |
gch | azo3510 | azo3509 | azo3510 | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | InterPro: Sulfatase Membrane protein; Specificity unclear. | 0.675 |
gch | exsB | azo3509 | azo0413 | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | Conserved hypothetical ExsB protein; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. | 0.992 |
gch | guaA | azo3509 | azo1581 | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | GMP synthase (glutamine-hydrolyzing); Catalyzes the synthesis of GMP from XMP. | 0.639 |
gch | queA | azo3509 | azo0908 | Probable GTP cyclohydrolase I; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). | S-adenosylmethionine:tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). | 0.630 |