Methionine synthase, homocysteine-binding module; Methionine synthase or 5-methyltetrahydrofolate-homocysteine S-methyltransferase catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate; L-homocysteine is bound via the zinc atom; Uses the cobalamin (vitamin B12) and zinc as cofactors; This sequence corresponds to the homocysteine-binding domain (Hcy-binding domain) only; Belongs to the vitamin-B12 dependent methionine synthase family; Localize [...]

Methionine synthase, cobalamin-binding module; Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.

The cysteine synthase is the enzyme responsible for the formation of cysteine from O-acetyl-serine and hydrogen sulfide with the concomitant release of acetic acid; Uses the pyridoxal phosphate as cofactor (vitamin B6) attached to a conserved lysine residue; Belongs to the cysteine synthase/cystathionine beta-synthase family; Localized in the cytoplasm; High confidence in function and specificity.

The cysteine synthase is the enzyme responsible for the formation of cysteine from O-acetyl-serine and hydrogen sulfide with the concomitant release of acetic acid; Uses the pyridoxal phosphate as cofactor (vitamin B6) attached to a conserved lysine residue; Belongs to the cysteine synthase/cystathionine beta-synthase family; Localized in the cytoplasm; High confidence in function and specificity.

Cystathionine beta-lyase (CBL) is found in the Selenoamino acids metabolism pathway and in methionine and cysteine biosynthesis pathways. It converts (seleno)cystathionine to (seleno)homocysteine but is also able to catalyze an alpha, gamma elimination; Uses a pyridoxal phosphate group as cofactor, attached to a lysine residue located in the central section of the enzyme; Localized in the cytoplasm; High confidence in function and specificity.

O-succinylhomoserine sulfhydrylase; Catalyzes the formation of L-homocysteine from O-succinyl-L- homoserine (OSHS) and hydrogen sulfide.

Cys/Met metabolism pyridoxal-phosphate-dependent enzymes; Transforms O-acetylhomoserine into L-methionine. Also reacts with other thiols and H2S, producing homocysteine or thioethers; Uses a pyridoxal phosphate group as cofactor, attached to a lysine residue located in the central section of the enzyme; Localized in the cytoplasm; High confidence in function and specificity.

DNA (cytosine-5-)-methyltransferase specifically methylates the C-5 carbon of cytosines in DNA to produce C5-methylcytosine. It uses S-adenosyl-L-methionine as methyl donor. This protein is highly; High confidence in function and specificity.

Homoserine O-acetyltransferase / Aspartokinase / Homoserine dehydrogenase; This enzyme is a modular protein with a homoserine O-acetyltransferase module (N-terminal) that catalyses the homoserine acetylation and belongs to the alpha/beta hydrolase fold family, an aspartate kinase module (central) that catalyzes the phosphorylation of aspartate and belongs to the amino acid kinase family and, in C- terminal part, a homoserine dehydrogenase module that catalyzes the NAD-dependent reduction of aspartate beta- semialdehyde into homoserine. This module is composed of a substrate-binding dom [...]