DNA polymerase III, beta chain; Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP- independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of r [...]

Conserved hypothetical periplasmic protein; Signal peptide cleaved between the resedues 31 and 32; Localized in the periplasmic space; Conserved hypothetical protein.

DNA polymerase IV; Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.

DNA polymerase IV; Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.

DNA ligase (polydeoxyribonucleotide synthase) joins two DNA fragments by catalysing the formation of an internucleotide phosphodiester bond between 5-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using ATP as a coenzyme and as the energy source. It is essential for DNA replication, DNA recombination and DNA repair; Belongs to the ATP-dependent DNA ligase family; Localized in the cytoplasm; High confidence in function and specificity.

The delta prime subunit of DNA polymerase III is part of the 'clamp' loading complex that assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork; The DNA polymerase III or replicase is responsible for most of the replicative synthesis in bacteria. It also exhibits a 3' to 5' exonuclease proofreading activity; Localized in the cytoplasm; High confidence in function and specificity.

Lipoprotein involved in the gliding motility; Signal peptide cleaved between the residues 17 and 18; localized in the cytoplasmic membrane; Function unclear.

The exonuclease III catalyses the cleavage in the 3'- to 5'-direction on double-stranded DNA to yield nucleoside 5'-phosphates. It also has an endonucleolytic activity near apurinic sites on DNA; Contains an Endonuclease/Exonuclease/phosphatase domains; Belongs to the DNA repair enzymes AP family 1; Localized in the cytoplasm; High confidence in function and specificity.

The exonuclease III catalyses the cleavage in the 3'- to 5'-direction on double-stranded DNA to yield nucleoside 5'-phosphates. It also has an endonucleolytic activity near apurinic sites on DNA; Contains an Endonuclease/ Exonuclease/phosphatase domains; Belongs to the DNA repair enzymes AP family 1; Localized in the cytoplasm; High confidence in function and specificity.