Nucleoside diphosphate kinase; Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate; Belongs to the NDK family.

Inosine-5'-monophosphate dehydrogenase; Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Belongs to the IMPDH/GMPR family.

Glutamate racemase; Provides the (R)-glutamate required for cell wall biosynthesis.

MazG is a nucleoside triphosphate pyrophosphohydrolase which can hydrolyze all eight of the canonical ribo- and deoxynucleoside triphosphates to their respective monophosphates and PP(i), with a preference for deoxynucleotides. MazG also interacts with Era, an essential GTPase; Localized in the cytoplasm; High confidence in function and specificity.

Stationary-phase survival protein surE; Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates; Belongs to the SurE nucleotidase family.

5'-Nucleotidase, phosphatase subunit; The 5'-Nucleotidase is involved in the degradation of extracellular 5'-nucleotides into membrane permeable nucleosides. Usually It is composed of a N-terminal phosphatase domain that provides the ligands to the dimetal cluster and a conserved histidine, which together form the catalytic site and of a C-terminal domain involved in the substrate binding; This lipoprotein corresponds only to the phosphatase domain that hydrolyses the phosphate esterified at carbon 5' of the ribose and deoxyribose portions of nucleotide molecules; Binds a chloride ion [...]

Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Glycerol kinase; Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn- glycerol 3-phosphate; Belongs to the FGGY kinase family.

Undecaprenyl pyrophosphate synthetase; Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.