Nucleoside-triphosphate diphosphatase; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. Belongs to the HAM1 NTPase family.

Glutaminase catalyzes the reaction: L-glutamine + H2O = L-glutamate + NH3. Localized in the cytoplasm; High confidence in function and specificity.

Glutamate dehydrogenase catalyzes the NAD- and/or NADP-dependent reversible deamination of L-glutamate into alpha-ketoglutarate. It is involved with either ammonia assimilation or glutamate catabolism. Forms a homohexamer and binds one NAD(P) cofactor by subunit. Localized in the cytoplasm; High confidence in function and specificity; Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Nitrilases, involved in the reduction of organic nitrogen compounds and ammonia production, are enzymes that convert nitriles into their corresponding acids and ammonia by cleavage of the carbon-nitrogen bonds. They acts on a wide range of aromatic nitriles including (indol- 3-yl)-acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amides; Belongs to the CN hydrolase family; Localized in the cytoplasm; Specificity unclear.

Cda is a calcium-dependent ATPase. Vanadate strongly inhibits its activity; Belongs to the alkaline phosphatase clan, type I phosphodiesterase family; Signal peptide cleaved between the residues 24 and 25; Localized in the periplasmic space; High confidence in function and specificity.

FtsK is a DNA motor protein, which is both required to move DNA out of the region of the septum during cell division and for the septum formation. Tracks DNA in an ATP-dependent manner by generating positive supercoils in front of it and negative supercoils behind it. It forms a hexamer. Features four transmembrane helices. Localized in the cytoplasmic membrane; High confidence in function and specificity.

UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase; Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. Belongs to the MurCDEF family. MurE subfamily.

Alanine racemase; Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids; Belongs to the alanine racemase family.

Glutamate synthase [NADPH] large chain; Glutamate synthase is a key enzyme in the early stages of the assimilation of ammonia. It is a complex iron-sulfur flavoprotein catalyzing the reductive transfer of the amido nitrogen from L-glutamine to 2-oxoglutarate to form two molecules of L-glutamate via intramolecular channelling of ammonia from the amidotransferase domain to the FMN-binding domain. Glutamate synthase forms an aggregate of 4 catalytic active heterodimers, consisting of a large and a small subunit (GltB). GltA binds as cofactors a 3Fe-4S cluster, a FAD and a FMN. Localized i [...]