L-threonine ammonia-lyase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.

COGs: COG1012 NAD-dependent aldehyde dehydrogenase; InterPro IPR016160:IPR015590:IPR005931; KEGG: sli:Slin_5523 delta-1-pyrroline-5-carboxylate dehydrogenase; PFAM: Aldehyde Dehydrogenase; SPTR: Delta-1-pyrroline-5-carboxylate dehydrogenase; TIGRFAM: delta-1-pyrroline-5-carboxylate dehydrogenase; PFAM: Aldehyde dehydrogenase family; TIGRFAM: delta-1-pyrroline-5-carboxylate dehydrogenase, group 1; Belongs to the aldehyde dehydrogenase family.

Xylose isomerase domain-containing protein TIM barrel; COGs: COG3622 Hydroxypyruvate isomerase; InterPro IPR012307:IPR017909:IPR006311; KEGG: dfe:Dfer_3054 xylose isomerase domain protein TIM barrel; PFAM: Xylose isomerase domain-containing protein TIM barrel; SPTR: Hydroxypyruvate isomerase; PFAM: Xylose isomerase-like TIM barrel; TIGRFAM: Tat (twin-arginine translocation) pathway signal sequence.

Translation elongation factor 2 (EF-2/EF-G); Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. [...]

Triosephosphate isomerase; Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D- glyceraldehyde-3-phosphate (G3P); Belongs to the triosephosphate isomerase family.

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.