| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| fhs | gcvT1 | PFREUD_04720 | PFREUD_15930 | Formate--tetrahydrofolate ligase; ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate; Belongs to the formate--tetrahydrofolate ligase family. | Glycine cleavage system T protein, aminomethyltransferase; S-Aminomethyldihydrolipoylprotein + Tetrahydrofolate <=> Dihydrolipoylprotein + 5,10-Methylenetetrahydrofolate + NH3. | 0.941 |
| fhs | glyA | PFREUD_04720 | PFREUD_18740 | Formate--tetrahydrofolate ligase; ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate; Belongs to the formate--tetrahydrofolate ligase family. | Glycine hydroxymethyltransferase precursor; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.977 |
| fhs | metE | PFREUD_04720 | PFREUD_10720 | Formate--tetrahydrofolate ligase; ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate; Belongs to the formate--tetrahydrofolate ligase family. | Methionine synthase, vitamin-B12 independent; 5-Methyltetrahydropteroyltri-L-glutamate + L-Homocysteine <=> Tetrahydropteroyltri-L-glutamate + L-Methionine. | 0.786 |
| fhs | metF | PFREUD_04720 | PFREUD_15670 | Formate--tetrahydrofolate ligase; ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate; Belongs to the formate--tetrahydrofolate ligase family. | 5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H; Belongs to the methylenetetrahydrofolate reductase family. | 0.412 |
| fhs | metH | PFREUD_04720 | PFREUD_11340 | Formate--tetrahydrofolate ligase; ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate; Belongs to the formate--tetrahydrofolate ligase family. | Methionine synthase (5-methyltetrahydrofolate:L-homocysteine S-methyltransferase); Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. | 0.969 |
| gcvT1 | fhs | PFREUD_15930 | PFREUD_04720 | Glycine cleavage system T protein, aminomethyltransferase; S-Aminomethyldihydrolipoylprotein + Tetrahydrofolate <=> Dihydrolipoylprotein + 5,10-Methylenetetrahydrofolate + NH3. | Formate--tetrahydrofolate ligase; ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate; Belongs to the formate--tetrahydrofolate ligase family. | 0.941 |
| gcvT1 | glyA | PFREUD_15930 | PFREUD_18740 | Glycine cleavage system T protein, aminomethyltransferase; S-Aminomethyldihydrolipoylprotein + Tetrahydrofolate <=> Dihydrolipoylprotein + 5,10-Methylenetetrahydrofolate + NH3. | Glycine hydroxymethyltransferase precursor; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.997 |
| gcvT1 | metF | PFREUD_15930 | PFREUD_15670 | Glycine cleavage system T protein, aminomethyltransferase; S-Aminomethyldihydrolipoylprotein + Tetrahydrofolate <=> Dihydrolipoylprotein + 5,10-Methylenetetrahydrofolate + NH3. | 5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H; Belongs to the methylenetetrahydrofolate reductase family. | 0.931 |
| gcvT1 | metH | PFREUD_15930 | PFREUD_11340 | Glycine cleavage system T protein, aminomethyltransferase; S-Aminomethyldihydrolipoylprotein + Tetrahydrofolate <=> Dihydrolipoylprotein + 5,10-Methylenetetrahydrofolate + NH3. | Methionine synthase (5-methyltetrahydrofolate:L-homocysteine S-methyltransferase); Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. | 0.954 |
| glyA | fhs | PFREUD_18740 | PFREUD_04720 | Glycine hydroxymethyltransferase precursor; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Formate--tetrahydrofolate ligase; ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate; Belongs to the formate--tetrahydrofolate ligase family. | 0.977 |
| glyA | gcvT1 | PFREUD_18740 | PFREUD_15930 | Glycine hydroxymethyltransferase precursor; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Glycine cleavage system T protein, aminomethyltransferase; S-Aminomethyldihydrolipoylprotein + Tetrahydrofolate <=> Dihydrolipoylprotein + 5,10-Methylenetetrahydrofolate + NH3. | 0.997 |
| glyA | metE | PFREUD_18740 | PFREUD_10720 | Glycine hydroxymethyltransferase precursor; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Methionine synthase, vitamin-B12 independent; 5-Methyltetrahydropteroyltri-L-glutamate + L-Homocysteine <=> Tetrahydropteroyltri-L-glutamate + L-Methionine. | 0.515 |
| glyA | metF | PFREUD_18740 | PFREUD_15670 | Glycine hydroxymethyltransferase precursor; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H; Belongs to the methylenetetrahydrofolate reductase family. | 0.951 |
| glyA | metH | PFREUD_18740 | PFREUD_11340 | Glycine hydroxymethyltransferase precursor; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | Methionine synthase (5-methyltetrahydrofolate:L-homocysteine S-methyltransferase); Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. | 0.953 |
| glyA | metK | PFREUD_18740 | PFREUD_11410 | Glycine hydroxymethyltransferase precursor; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | S-adenosylmethionine synthetase (Methionine adenosyltransferase) (AdoMet synthetase) (MAT); Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. | 0.742 |
| glyA | thrA/hom | PFREUD_18740 | PFREUD_10320 | Glycine hydroxymethyltransferase precursor; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | L-Homoserine + NAD+ <=> L-Aspartate 4-semialdehyde + NADH + H+ and L-Homoserine + NADP+ <=> L-Aspartate 4-semialdehyde + NADPH + H+. | 0.475 |
| luxS | malY | PFREUD_11050 | PFREUD_22880 | S-ribosylhomocysteine lyase (Autoinducer-2 production protein luxS) (AI-2 synthesis protein); Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD). Belongs to the LuxS family. | Cysteine-S-conjugate beta-lyase; L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate. | 0.913 |
| luxS | metB | PFREUD_11050 | PFREUD_18590 | S-ribosylhomocysteine lyase (Autoinducer-2 production protein luxS) (AI-2 synthesis protein); Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD). Belongs to the LuxS family. | Cystathionine gamma-synthase (O-succinylhomoserine (thiol)-lyase). | 0.951 |
| luxS | metE | PFREUD_11050 | PFREUD_10720 | S-ribosylhomocysteine lyase (Autoinducer-2 production protein luxS) (AI-2 synthesis protein); Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD). Belongs to the LuxS family. | Methionine synthase, vitamin-B12 independent; 5-Methyltetrahydropteroyltri-L-glutamate + L-Homocysteine <=> Tetrahydropteroyltri-L-glutamate + L-Methionine. | 0.645 |
| luxS | metH | PFREUD_11050 | PFREUD_11340 | S-ribosylhomocysteine lyase (Autoinducer-2 production protein luxS) (AI-2 synthesis protein); Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD). Belongs to the LuxS family. | Methionine synthase (5-methyltetrahydrofolate:L-homocysteine S-methyltransferase); Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. | 0.967 |
