| node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
| CBL55977.1 | CBL56066.1 | PFREUD_04430 | PFREUD_05350 | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | Pyruvate flavodoxin/ferredoxin oxidoreductase. | 0.933 |
| CBL55977.1 | ilvB | PFREUD_04430 | PFREUD_13410 | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | Acetolactate synthase, large subunit, biosynthetic type; 2-Acetolactate + CO2 <=> 2 Pyruvate - Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. | 0.867 |
| CBL55977.1 | ilvC | PFREUD_04430 | PFREUD_13390 | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | Ketol-acid reductoisomerase (NADP(+)); Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.810 |
| CBL55977.1 | ilvN | PFREUD_04430 | PFREUD_13400 | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | Acetolactate synthase subunit small; 2-Acetolactate + CO2 <=> 2 Pyruvate - Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. | 0.992 |
| CBL55977.1 | leuA1 | PFREUD_04430 | PFREUD_08920 | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. | 0.878 |
| CBL55977.1 | leuA2 | PFREUD_04430 | PFREUD_13340 | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | (2S)-2-Isopropylmalate + CoA <=> Acetyl-CoA + 3-Methyl-2-oxobutanoic acid + H2O; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 0.878 |
| CBL55977.1 | leuB | PFREUD_04430 | PFREUD_13360 | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | (2R,3S)-3-Isopropylmalate + NAD+ <=> (2S)-2-Isopropyl-3-oxosuccinate + NADH + H+. | 0.468 |
| CBL55977.1 | nifJ1 | PFREUD_04430 | PFREUD_01840 | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin, which reduces nitrogenase, Pyruvate + CoA + oxidized flavodoxin = acetyl-CoA + CO2 + reduced flavodoxin. | 0.965 |
| CBL55977.1 | nifJ2 | PFREUD_04430 | PFREUD_07070 | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | Pyruvate:ferredoxin (Flavodoxin) oxidoreductase. | 0.963 |
| CBL56066.1 | CBL55977.1 | PFREUD_05350 | PFREUD_04430 | Pyruvate flavodoxin/ferredoxin oxidoreductase. | Thiamine pyrophosphate enzyme; Belongs to the TPP enzyme family. | 0.933 |
| CBL56066.1 | ilvB | PFREUD_05350 | PFREUD_13410 | Pyruvate flavodoxin/ferredoxin oxidoreductase. | Acetolactate synthase, large subunit, biosynthetic type; 2-Acetolactate + CO2 <=> 2 Pyruvate - Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. | 0.933 |
| CBL56066.1 | ilvN | PFREUD_05350 | PFREUD_13400 | Pyruvate flavodoxin/ferredoxin oxidoreductase. | Acetolactate synthase subunit small; 2-Acetolactate + CO2 <=> 2 Pyruvate - Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. | 0.915 |
| CBL56066.1 | leuA1 | PFREUD_05350 | PFREUD_08920 | Pyruvate flavodoxin/ferredoxin oxidoreductase. | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. | 0.929 |
| CBL56066.1 | leuA2 | PFREUD_05350 | PFREUD_13340 | Pyruvate flavodoxin/ferredoxin oxidoreductase. | (2S)-2-Isopropylmalate + CoA <=> Acetyl-CoA + 3-Methyl-2-oxobutanoic acid + H2O; Belongs to the alpha-IPM synthase/homocitrate synthase family. | 0.929 |
| CBL56066.1 | nifJ1 | PFREUD_05350 | PFREUD_01840 | Pyruvate flavodoxin/ferredoxin oxidoreductase. | Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin, which reduces nitrogenase, Pyruvate + CoA + oxidized flavodoxin = acetyl-CoA + CO2 + reduced flavodoxin. | 0.999 |
| CBL56066.1 | nifJ2 | PFREUD_05350 | PFREUD_07070 | Pyruvate flavodoxin/ferredoxin oxidoreductase. | Pyruvate:ferredoxin (Flavodoxin) oxidoreductase. | 0.999 |
| ilvA | ilvB | PFREUD_03750 | PFREUD_13410 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acetolactate synthase, large subunit, biosynthetic type; 2-Acetolactate + CO2 <=> 2 Pyruvate - Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. | 0.978 |
| ilvA | ilvC | PFREUD_03750 | PFREUD_13390 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Ketol-acid reductoisomerase (NADP(+)); Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.844 |
| ilvA | ilvN | PFREUD_03750 | PFREUD_13400 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Acetolactate synthase subunit small; 2-Acetolactate + CO2 <=> 2 Pyruvate - Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. | 0.984 |
| ilvA | leuA1 | PFREUD_03750 | PFREUD_08920 | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. | 0.493 |
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