Possible transcriptional regulatory protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines; Belongs to the Dus family.

Possible linoleoyl-CoA desaturase (delta(6)-desaturase); Is likely involved in the aerobic desaturation system responsible for the synthesis of oleic acid from stearoyl-CoA; oleic acid is a precursor of mycobacterial membrane phospholipids and triglycerides. Catalyzes the conversion of stearoyl-CoA to oleoyl-CoA by introduction of a cis double bond between carbons 9 and 10 of the acyl chain. Requires the electron transfer partner Rv3230c to pass two electrons from NADPH to its active site diiron center. Is also able to catalyze the 9-desaturation of palmitoyl-CoA to palmitoleoyl-CoA.

Rv2524c, (MTCY159.32, MTV009.09c), len: 3069 aa. Probable fas, Fatty Acid Synthase, equivalent to Q9X7E2|fas|ML1191 putative type I fatty acid synthase from Mycobacterium leprae (3076 aa), FASTA scores: opt: 17484,E(): 0, (85.8% identity in 3081 aa overlap). Also similar to others e.g. Q04846|fas|Q59497 from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (3104 aa), FASTA scores: opt: 3981, E(): 5.5e-203, (49.8% identity in 3099 aa overlap); Q48926|fas from Mycobacterium bovis (2796 aa),FASTA scores: opt: 2098, E(): 3.9e-103, (59.7% identity in 2862 aa overlap) (see Fernande [...]

Hypothetical oxidoreductase; Is likely involved in the aerobic desaturation system responsible for the synthesis of oleic acid from stearoyl-CoA; oleic acid is a precursor of mycobacterial membrane phospholipids and triglycerides. Is the electron transfer partner for the stearoyl-CoA 9- desaturase DesA3. Catalyzes electron transfer reaction between NADPH and the diiron center of DesA3. Cannot use NADH.

Probable ferredoxin FdxC; Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.

Probable NrdI protein; Probably involved in ribonucleotide reductase function.

Ribonucleoside-diphosphate reductase subunit alpha; Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. When coexpressed in E.coli with nrdF2 the 2 proteins complement a temperature-sensitive E.coli mutant, however coexpression with nrdF1 does not complement. Belongs to the ribonucleoside diphosphate reductase large chain family.

Probable ribonucleoside-diphosphate reductase (large subunit) NrdZ (ribonucleotide reductase); Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).

Conserved protein; Rv0825c, (MTV043.17c), len: 213 aa. Conserved protein, highly similar, but in part (between aa ~43-96) to fadD27|Rv0275c|MTV035.03 putative fatty-acid-CoA ligase from Mycobacterium tuberculosis (241 aa), FASTA scores: E(): 7.3e-09, (32.6% identity in 190 aa overlap). Also shows similarity with other proteins from Mycobacterium tuberculosis e.g. Rv0078|AL0214|MTV030_22 (201 aa), FASTA scores: opt:118, E(): 0.32, (34.5% identity in 113 aa overlap); etc.