Rv0218, (MTCY08D5.13), len: 442 aa. Probable conserved transmembrane protein, some similarity with sulfite oxidases e.g. SUOX_HUMAN|P51687 sulfite oxidase precursor (488 aa), FASTA scores: opt: 153, E(): 0.0087,(28.6% identity in 161 aa overlap); and with some nitrate reductases e.g. NIA_FUSOX|P39863 nitrate reductase (NADPH) (905 aa), FASTA scores: opt: 143, E(): 0.06, (29.3% identity in 92 aa overlap). Also similar to BSUB0017_86 from Mycobacterium tuberculosis.

Alpha-mannosidase; Rv0648, (MTCY20H10.29), len: 1215 aa. Alpha-mannosidase (see citation below), showing some similarity to hypothetical proteins and various sugar hydrolases e.g. SYCSLRA_6|Q55528 hypothetical 1 20.4 kDa protein from Synechocystis (1042 aa), FASTA scores: opt: 260, E(): 3.6e-08, (23.4% identity in 602 aa overlap); etc. Contains PS00659 Glycosyl hydrolases family 5 signature.

Rv2265, (MTCY339.45c), len: 409 aa. Possible conserved integral membrane protein, with some similarity to others e.g. M. thermoauto. sp|O26855|O26855 conserved protein (383 aa), FASTA score: opt: 898 z-score: 1023.5 E(): 0; 38.0% identity in 384 aa overlap; Q58713 hypothetical 44.1 kDa protein 1 317 (398 aa), FASTA scores,opt: 305 E(): 1.2e-11; 22.8% identity in 382 aa overlap; also KGTP_ECOLI P17448 alpha-ketoglutarate permease (432 aa), FASTA scores, opt: 156, E(): 0.006, (24.8% identity in 416 aa overlap).

Rv0522, (MTCY20G10.12), len: 434 aa. Probable gabP,GABA permease (gamma-aminobutyrate permease), integral membrane protein, highly similar to others e.g. GABP_ECOLI|P25527 gaba permease from Escherichia coli (466 aa), FASTA scores: opt: 1218, E(): 0, (44.3% identity in 424 aa overlap); etc. Also similar to other M. tuberculosis permeases e.g. MTCY13E10.06c FASTA score: (34.4% identity in 407 aa overlap). Contains PS00218 Amino acid permeases signature. Overlaps and extends Rv0523c|MTCY25D10.01 from overlapping cosmid. Belongs to the amino acid permease family (APC family).

Conserved protein; Rv1998c, (MTCY39.20), len: 258 aa. Conserved protein, showing some similarity with other hypothetical proteins e.g. U82823|SEU82823.03 Saccharopolyspora erythraea (266 aa), FASTA results: opt: 654, E(): 0, (43.8% identity in 249 aa overlap); and AL034446|SC1A9.07 Streptomyces coelicolor (251 aa), FASTA scores: opt: 592,E(): 1.5e-31, (43.4% identity in 251 aa overlap).

Trehalose synthase TreS; Catalyzes the reversible interconversion of maltose and trehalose by transglucosylation. Also displays amylase activity, catalyzing the endohydrolysis of (1->4)-alpha-D- glucosidic linkages in glycogen and maltooligosaccharides such as maltoheptaose, to produce maltose which then can be converted to trehalose. TreS plays a key role in the utilization of trehalose for the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P). Might also function as a sensor and/or regulator of trehalose levels [...]

Probable glucanase GlgE; Essential maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)- glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP5 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Exhibits an alpha-retaining catalytic mechanism. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen in the presence [...]

Alpha-glucosidase AglA; Rv2471, (MTV008.27), len: 546 aa. Probable aglA,maltase (alpha-glucosidase), highly similar or similar to several e.g. Q60027|AGLA from Thermomonospora curvata (544 aa), FASTA scores: opt: 2071, E(): 4e-116, (57.7% identity in 525 aa overlap); Q9KZE3|AGLAE from Streptomyces coelicolor (534 aa), FASTA scores: opt: 1475, E(): 1.5e-80,(50.1% identity in 537 aa overlap); O86874|AGLA from Streptomyces lividans (534 aa), FASTA scores: opt: 1473,E(): 2e-80, (50.1% identity in 537 aa overlap); etc. Seems to belong to family 13 of glycosyl hydrolases, also known as the a [...]