Phosphatidylinositol phosphate synthase; Catalyzes the conjugation of the 1'-hydroxyl group of D-myo- inositol-3-phosphate (also named L-myo-inositol-1-phosphate) with a lipid tail of cytidine diphosphate diacylglycerol (CDP-DAG), forming phosphatidylinositol phosphate (PIP) and CMP. PIP is a precursor of phosphatidylinositol (PI) which is an essential lipid for mycobacteria required for formation of their cell wall.

Probable threonyl-tRNA synthetase ThrS (threonine-tRNA synthetase)(ThrRS) (threonine-tRNA ligase); Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).

Alpha-mannosyltransferase PimA; Involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM). Catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). PimA plays an essential role for growth in macrophages and during both the acute and chronic phases of infection.

Rv2609c, (MTCY01A10.24), len: 351 aa. Probable conserved membrane protein, equivalent to O07146|MLCL581.13c|ML0451 hypothetical 37.9 KDA protein from Mycobacterium leprae (349 aa), FASTA scores: opt: 1675, E(): 1.4e-95, (77.85% identity in 334 aa overlap). Also similar to hypothetical proteins: O69888|SC2E1.17|mutt hypothetical 19.4 KDA protein from Streptomyces coelicolor and Streptomyces lividans (172 aa), FASTA scores: opt: 345,E(): 3.5e-14, (44.7% identity in 161 aa overlap); Q9L285|SCL2.14c hypothetical 19.8 KDA protein from Streptomyces coelicolor (180 aa), FASTA scores: opt: 179 [...]

Sulfate adenylyltransferase subunit 1; ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity; In the C-terminal section; belongs to the APS kinase family.

Rv1285, (MTCY373.04), len: 332 aa. Probable cysD,sulfate adenylyltransferase subunit 2 (see Wooff et al.,2002), homology suggests start site at aa 24 or 28, similar to e.g. CYSD_ECOLI|P21156 sulfate adenylate transferase subunit 2 from Escherichia coli (302 aa), FASTA score: opt: 973, E():0, (52.5% identity in 303 aa overlap). Also similar to Mycobacterium tuberculosis Rv2392,3'-phosphoadenylylsulfate reductase. Belongs to the PAPS reductase family. CYSD subfamily. Thought to be differentially expressed within host cells (see Triccas et al., 1999).

Conserved protein; Rv2257c, (MTV022.07c), len: 272 aa. Conserved protein, similar to hypothetical protein SC4A7.08 from Streptomyces coelicolor (273 aa; 58% identity in 243 aa overlap). Also similar to several putative esterases and penicillin-binding proteins in M. tuberculosis e.g. Rv1923,Rv1497, Rv2463, Rv3775, Rv1922, Rv1730c.

Conserved protein; Rv1209, (MTCI364.21), len: 122 aa. Conserved protein, containing a hydrophobic N-terminus. Similar to Q49956|U1756M hypothetical protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 524, E(): 8.9e-29,(78.6% identity in 112 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).

DNA-directed RNA polymerase subunit omega; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.